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Method for remedying soil contaminated by DDT as pesticide residue through earthworm-based bioaugmentation and biodegradation

A bioaugmentation and pesticide residue technology, applied in the field of environmental biochemistry, can solve problems such as earthworm soil pollution, and achieve simple and effective restoration, accelerated decomposition, accelerated elimination and detoxification

Inactive Publication Date: 2010-09-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the reports on the role of earthworms in soil pollution, especially the remediation of organochlorine pesticide DDT pollution are very limited, and further research is needed

Method used

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  • Method for remedying soil contaminated by DDT as pesticide residue through earthworm-based bioaugmentation and biodegradation
  • Method for remedying soil contaminated by DDT as pesticide residue through earthworm-based bioaugmentation and biodegradation
  • Method for remedying soil contaminated by DDT as pesticide residue through earthworm-based bioaugmentation and biodegradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1. Effect test of different earthworm stocking densities

[0037] The soil for testing was collected from vegetable garden soil. The soil samples were fully air-dried at room temperature, then mixed and pulverized, and passed through a 2mm sieve. The earthworm species are selected from Eisenia chinensis (surface species) and Esperus magnifolia (inner species).

[0038] Experimental design Add 5 (A), 10 (B), 15 (C), 20 (D), 25 (E), 30 (F) and 35 (G) earthworms of two species , 40 (H), 45 (I), 50 (L) totally 20 processing samples. Each treated soil sample weighs 1kg, and 500g of fermented cow dung is added to each treatment. After the cow dung is ground, it is fully mixed with the soil. Every three months, 500g of the fermented cow dung that has been ground and fermented is put in again, and fully mixed with the soil. The water content of the soil was kept at 55-65% during the restoration process, and each treatment was repeated three times. The experimental...

Embodiment 2

[0040] Example 2. The earthworm feeding effect test when the quality of fermented chicken manure and / or cow manure accounts for different percentages of the total mass

[0041]The soil to be tested was paddy soil, and the soil samples were fully air-dried at room temperature, then mixed and pulverized, and passed through a 2mm sieve. The earthworm species are selected from Eisenia chinensis (surface species) and Esperus magnifolia (inner species).

[0042] Experimental Design Two kinds of earthworms respectively add the quality of fermented chicken manure and / or cow manure to account for 5% (A), 10% (B), 15% (C), 20% (D), 25% ( E), 30% (F), 40% (G), 60% (H), 80% (I), 100% (L) a total of 20 treatments. The weight of each treatment sample is 1kg. The worms used in the experiment are 30 worms for the treatment of Eisenia chinensis, and 15 worms for the treatment of P. , and fully mixed with the soil, the soil moisture content was kept at 55-65% during the restoration process, a...

Embodiment 3

[0044] Embodiment 3. Different earthworm species, density are tested to DDT biodegradation effect

[0045] The experimental design weighs 2kg for each soil sample. The soil for testing is collected from the surface soil (0-20cm) near the green banyan circle (23°09.190N, 113°21.244E) of South China Agricultural University. For red soil, the content of pesticides in the soil is negligible. The soil samples were fully air-dried at room temperature, then mixed and pulverized, and passed through a 2mm sieve. As earthworm species, Eisenia chinensis (surface species, serial number: E) and Cyclochaetos grandiosa (inner species, serial number: A) were selected. The concentration of DDT in the soil is 2mg / kg (code: C1) and 4mg / kg (code: C2). Since it is difficult to ensure the uniformity of the sample directly by adding DDT to the soil, this experiment first dissolves DDT in ethanol (2mg / kg kg treatment shared DDT0.08g, ethanol 10mL; 4mg / kg treatment shared DDT0.16g, ethanol 20mL), the...

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Abstract

The invention discloses a method for remedying the soil contaminated by DDT (1,1,1-trichloro-2,2-bis-(pchlorophenyl)ethane) as a pesticide residue through earthworm-based bioaugmentation and biodegradation. The method comprises the following steps: feeding eiseniafoetida or amynthas rubusto in the contaminated soil; further feeding fermented chicken manure and / or cattle manure and fully and evenly mixing the chicken manure and / or cattle manure and the soil; and feeding the fermented chicken manure and / or cattle manure every three months, wherein the feeding density of the eiseniafoetida or amynthas robust is 5 to 30 earthworms per each kilogram of soil; the mass ratio between the chicken manure and / or cattle manure and the soil is 0.2-1:1; and the water content of the soil in the soil remediation is maintained at 55% to 65%. The invention has the advantage that the requirements for the external environment are low, so the invention is widely applicable to most of the soil contaminated by the DDT at current; and the method for remedying the soil contaminated by DDT as an organic chlorine pesticide is simple, effective and low-cost.

Description

technical field [0001] The invention belongs to the technical field of environmental biochemistry, and in particular relates to the repair and treatment of polluted soil, in particular to a method for repairing DDT polluted soil. Background technique [0002] As a synthetic organochlorine pesticide, DDT[1,1,1-trichloro-2,2-bis-(pchlorophenyl)ethane] has made a significant contribution to the world's food production, but it has also caused serious damage to the atmosphere, water and Serious pollution of the soil environment. DDT has the characteristics of high persistence, broad-spectrum toxicity, lipophilicity and semi-volatility, and is a typical persistent organic pollutant (POPs). Although most countries in the world stopped producing and using DDT as early as the 1970s and 1980s, some of the DDT applied to farmland still remained in the soil environment, and its detection rate was still high. Therefore, the pollution of DDT to the soil has not disappeared because of it...

Claims

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Application Information

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IPC IPC(8): B09C1/00
Inventor 李永涛蔺中李芳柏黄德银戴军
Owner SOUTH CHINA AGRI UNIV
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