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88results about How to "Instant detection" patented technology

Sample pretreatment method for detection of organochlorine pesticide residue in food

The invention discloses a sample pretreatment method for detection of organochlorine pesticide residue in food and is characterized by comprising the following steps: water is added to a food sample, sample solution is swirled, and then inorganic salt and hydrophilic organic solvent are added to the sample solution, the sample solution is swirled and shaken uniformly, the sample solution undergoes phase splitting after centrifugation at room temperature, the upper-phase liquid is purified through dispersion solid-phase extraction method, the extraction temperature is 20-30 DEG C, and finally the upper-phase liquid is detected by gas chromatography; and the mass percent of the inorganic salt accounts for 5%-35% of the total mass of the system, the mass percent of the hydrophilic organic solvent accounts for 10%-50% of the total mass of the system, and water is of the residual amount, and the total mass of the system is the mass sum of water contained in the sample, the added water, the inorganic salt and the hydrophilic organic solvent. According to the sample pretreatment method, a dual-water-phase system consisting of the hydrophilic organic solvent, the organic salt and water is utilized, the partition coefficient is increased by means of changing the types and concentrations of the organic solvent and the inorganic salt, and the organochlorine pesticide residue in the sample can be directly extracted at room temperature.
Owner:YANTAI UNIV

Colloidal gold detection card for rapidly detecting melamine and preparing method thereof

The invention provides a colloidal gold cassette for rapid detection of melamine, the colloidal gold cassette has rapid detection, convenient carry and simple operation, the invention also provides a preparation method of the cassette, the preparation process thereof is simple, and the cost is low. The colloidal gold cassette comprises a test strip, the test strip is packaged in a shell body of the cassette, a sample adding hole and an observation hole are arranged on the shell body of the cassette, the test strip comprises a bottom plate, the colloidal gold cassette is characterized in that water absorbent paper, a nitrocellulose membrane, a gold-labeled pad and a sample pad are sequentially arranged on the bottom plate of the test strip, the water absorbent paper and the gold-labeled pad are respectively arranged at the two ends of the nitrocellulose membrane, small sections of the water absorbent paper and the gold-labeled pad are respectively overlapped on the nitrocellulose membrane at the junctions at the two ends of the nitrocellulose membrane, the sample pad is arranged at the other end of the gold-labeled pad, a small section of the sample pad is overlapped on the gold-labeled pad; a gold-labeled anti-melamine monoclonal antibody is sprayed on the gold-labeled pad; a detection line with the material of a melamine-ovalbumin coupled conjugate and a quality control line with the material of goat anti-mouse IgG are sequentially sprayed on the nitrocellulose membrane.
Owner:江苏省苏微微生物研究有限公司

hCG cycle detection test paper, hCG cycle detection kit, and preparation method and applications of hCG cycle detection test paper

The invention discloses hCG cycle detection test paper, which comprises a first test paper strip and a second test paper strip, wherein the first test paper strip and the second test paper strip respectively comprise a substrate, a sample loading pad, a colloidal gold adsorption pad, an antibody carrying film and a water absorption pad, the sample loading pad, the colloidal gold adsorption pad, the antibody carrying film and the water absorption pad are sequentially adhered to the substrate, the antibody carrying film is provided with a detection line and a quality control line at intervals, the colloidal gold adsorption pad on the first test paper strip adsorbs a colloidal gold-anti-human chorionic gonadotropin beta-hCG monoclonal antibody conjugate, the colloidal gold adsorption pad on the second test paper strip adsorbs a colloidal gold-anti-human chorionic gonadotropin beta-hCG monoclonal antibody conjugate and free beta antibody, the detection line is coated with alpha-hCG monoclonal antibody, and the quality control line is coated with an anti-mouse IgG polyclonal antibody. According to the present invention, the detection test paper does not need to treat the urine sample, has characteristics of simple operation and simple detection, is suitable for on-site, instant and rapid detection, and is suitable for the detection of gestational weeks in the family.
Owner:NANTONG EGENS BIOTECH CO LTD

Paper chip aptamer sensor and preparation method thereof

The invention relates to a paper chip aptamer sensor and a preparation method thereof. The paper chip aptamer sensor comprises four layers of filter paper, microfluidic channels (1), filter membranes(2), reaction zones (3), counter electrodes (4), reference electrodes (5) and working electrode (6); the four layers of filter paper are a first layer of filter paper, a second layer of filter paper,a third layer of filter paper and a fourth a layer of filter paper which are distributed sequentially from top to bottom; the microfluidic channels (1) are disposed on the first layer of filter paperand the third layer of filter paper; the filter membranes (2) are disposed on the first layer of filter paper, the second layer of filter paper, and the third layer of filter paper, and are communicated with the microfluidic channels (1); the reaction zones (3) are arranged on the third layer of filter paper and are communicated with the microfluidic channels (1) on the third layer of filter paper; the counter electrodes (4) and the reference electrodes (5) are disposed on the second layer of filter paper and are located above the reaction zones (3); the working electrodes (6) are disposed onthe fourth layer of filter paper and are located below the reaction zones (3); the surfaces of the working electrodes (6) are modified with a nanocomposite and are fixedly provided with aptamers.
Owner:INST OF ELECTRONICS CHINESE ACAD OF SCI

Self-adjusting system and method for PM 2.5 flue gas flow fields

ActiveCN105135897AInstant control opening and closingInstant control steeringAerodynamic testingControl devices for furnacesControl flowAutomatic control
The invention relates to a self-adjusting system and method for PM 2.5 flue gas flow fields. The self-adjusting system is characterized by comprising flue gas detection devices, an online monitoring device and an automatic-control adjusting device; the flue gas detection devices are distributed at all detection points of a flue gas pipeline and a dust removal box and fixed to the inner wall of the flue gas pipeline and the inner wall of the dust removal box through supporting legs, detection heads of the flue gas detection devices are exposed to the flue gas pipeline by different heights for sampling, and the flue gas detection devices are connected with the online monitoring device and transmit detection and analysis results to a center processing unit of the online monitoring device; the center processing unit controls flow guide plates in the flue gas pipeline to turn and an air pressure pump in the dust removal box to be turned on or off through a real-time synchronous control system of the automatic-control adjusting device according to the distribution condition of the flue gas flow fields. The self-adjusting system and method can detect PM2.5 in a distributed mode or on line, and the flow fields in the flue gas pipeline and the dust removal box can be controlled intelligently.
Owner:HUNAN CHANGZHONG MACHINERY

Device and method capable of detecting stripes of optical glass prefabricated member

The invention provides a stripe detection device and a stripe detection method capable of rapidly detecting the stripes of ellipsoidal aspheric pre-compressed prefabricated member. The device for detecting the stripes of the optical glass prefabricated member comprises a laser light source, a beam expanding system and a CCD image processing system in sequence according to a light path, wherein the CCD image processing system comprises a lens and a computer. According to the device, the strips at the inner part of the ellipsoidal aspheric pre-compressed prefabricated member are detected immediately through a stripe interference principle, and the relatively high detection precision can be achieved; the device structure is compact, the occupying space is small, the degree of automation is high, the resolution ratio effect is good, and the purpose of immediately rapidly detecting the stripes of the ellipsoidal aspheric pre-compressed prefabricated member can be achieved. According to the method, the glass does not need to be annealed and subjected to cold processing, a product can be detected immediately, the result can be obtained, site production process and parameters can be regulated timely, and the method can be implemented in an indoor limited space, the operation is convenient, and the detection efficiency is high.
Owner:CDGM GLASS LLC

Vibrio qinghaiensis Q67B and separation, screening and application thereof

The invention relates to a vibrio qinghaiensis Q67B (CCTCC (China Center for Type Culture Collection) NO: M2010104) and separation, screening and an application of vibrio qinghaiensis Q67B, and belongs to the technical field of the separation, the screening and the application of luminous bacteria of a microbiology. Vibrio qinghaiensis Q67B is a freshwater luminous bacterium by separating and purifying luminous bacteria collected on the body surface of Qinghai kaluga produced from Qinghai Lake, Qinghai Province, and screening through temperature adaptability, then screening through pH value adaptability, and finally screening through poison reaction sensitivity. Vibrio qinghaiensis Q67B is constantly luminous in a clean water body, when the bacterium contacts poison, the luminous intensity is obviously reduced, so the toxicity and concentration of the poison can be judged. When vibrio qinghaiensis Q67B is used for poison detection of surface water such as inland lakes and rivers, groundwater and sewage effluent, the poison detection can be carried out at 10-37 DEG C without adjusting an osmotic pressure and a pH value of water sample, and the poison of the water sample can be distinguished in 10-15min, so that vibrio qinghaiensis Q67B has the advantages of speediness, convenience and sensitivity. Ocean luminous bacteria do not have the above advantages.
Owner:LIHE TECH (HUNAN) CO LTD

LAMP primer composition and kit for detecting swine acute diarrhea syndrome coronavirus by using microfluidic chip technology

The invention belongs to the field of nucleic acid detection and diagnosis, and in particular relates to a loop-mediated isothermal amplification (LAMP) primer composition and kit for detecting a swine acute diarrhea syndrome coronavirus (SADS-CoV) by using a microfluidic chip technology. In order to solve the defect of time-consuming, laborious and high-cost detection of a swine acute diarrhea syndrome coronavirus, LAMP primers are designed according to a relatively conservative M gene of the SADS-CoV, after screening is performed, the LAMP primer composition for detecting the swine acute diarrhea syndrome coronavirus is obtained. The invention also provides the kit combining the microfluidic chip technology and an LAMP technology and a detection method of the SADS-CoV by using the kit. The composition and kit are applied to detection of the swine acute diarrhea syndrome coronavirus, and can realize the rapid and immediate detection of the swine acute diarrhea syndrome coronavirus, thereby solving the defect of time-consuming, laborious and high-cost detection of the swine acute diarrhea syndrome coronavirus; and the composition and kit improve the detection sensitivity and specificity, reduce labor and equipment costs, and shorten the detection cycle.
Owner:SOUTH CHINA AGRI UNIV

Antibacterial type food freshness indication material capable of detecting carbon dioxide and preparation method thereof

The invention discloses an antibacterial type food freshness indication material capable of detecting carbon dioxide. The antibacterial type food freshness indication material is prepared from a membrane solution A, a membrane solution B and a membrane solution C in parts by weight according to a ratio of 2 to (1 to 1.5) to (0.1 to 0.3). The antibacterial type food freshness indication material isprepared by the steps of blending the membrane solution A, the membrane solution B and the membrane solution C, performing ultrasonic and heating treatment, paving on a carrier, performing vacuum drying operation and peeling a membrane to obtain the antibacterial type food freshness indication material. The membrane solution A is prepared from the following raw materials of starch, titanium dioxide dispersion liquid, calcium hydroxide, glycerin, urea, xanthan gum and water; the membrane solution B is prepared from the following raw material of a polyvinyl alcohol solution; the membrane solution C is prepared from the following raw materials of a methyl red solution and a bromothymol blue solution. The material can inhibit growth of bacteria in food and can detect food freshness. The invention further provides a preparation method of the antibacterial type food freshness indication material capable of detecting carbon dioxide.
Owner:JILIN AGRICULTURAL UNIV

Biosensor for detecting okadaic acid and preparation method

The invention relates to a biosensor for detecting okadaic acid. The biosensor comprises an insulated substrate and an electrode system, wherein the electrode system is formed on the substrate and comprises a working electrode, a reference electrode and a counter electrode; and a biological enzyme layer is fixed on the working electrode and comprises protein phosphatase 2A (PP2A enzyme) and an electron transport complex. The invention further relates to a preparation method of the biosensor, and mainly relates to fixation of the PP2A enzyme. Compared with the prior art, the biosensor taking the PP2A enzyme as a fixed enzyme layer is prepared by utilizing the inhibition effect of okadaic acid to the activity of the PP2A enzyme; the content of okadaic acid in a sample is determined according to the activity inhibiting degree of the PP2A enzyme, namely, the concentration of okadaic acid in the sample is converted into an electric signal to be detected, and therefore, the detection result is visual; simultaneously, by virtue of the specificity and the high efficiency of PP2A enzyme catalytic reaction, the detection process is high in specificity, the accuracy rate of a detection structure is high, the detection period is short, and instant and rapid detection requirements can be met.
Owner:ZHEJIANG OCEAN UNIV

Low abundance hormone detection method and device

The invention discloses a low abundance hormone detection method and device. A device for executing detection is provided with a multi-layer structure. Electrophoresis through hole regions of each layer structure are communicated to form an electrophoresis channel. The method comprises the steps that a trailing electrolyte and to-be-detected liquid are dropped on the surface of a top layer of the device; a leading electrolyte is dropped on the surface of a bottom layer of the device; electrophoresis is started by applying direct current voltage between the top layer and the bottom layer, so that the trailing electrolyte and the to-be-detected liquid are migrated in the direction of the bottom layer along the electrophoresis channel, and the leading electrolyte is migrated in the direction of the top layer along the electrophoresis channel; during electrophoresis, when to-be-detected hormone in the to-be-detected liquid is migrated and gathered on an enrichment layer of the device, and electrophoresis is stopped by removing the direct current voltage applied between the top layer and the bottom layer; the to-be-detected hormone gathered on the enrichment layer is moved to a detection region of a detection layer of the device, and electrochemical detection is performed on the detection region, and the concentration of the to-be-detected hormone is determined.
Owner:PEKING UNIV FIRST HOSPITAL +1

Clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper based on nano antibody, and preparation method thereof

The invention discloses clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper based on a nano antibody, and a preparation method of the clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper. According to the clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper, a gold-labeled pad in the detection test paper adsorbs a colloidal gold nanoparticle labeled anti-clenbuterol hydrochloride small molecule hapten nano antibody Nb; a detection line T on a detection membrane is a conjugate BSA-CL blot of clenbuterol hydrochloride micromolecule CL and bovine serum albumin BSA, and a quality control line C on the detection membrane is an anti-BSA protein rabbit-derived polyclonal antibody pAb blot. The invention further specifically discloses a preparation method of the clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper based on the nano antibody. The clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper based on the nano antibody prepared by adopting the preparation method is simple and convenient in detection operation, high in specificity, high in sensitivity, capable of rapidly giving a visual result and easy to popularize and apply in life practice.
Owner:XINXIANG MEDICAL UNIV
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