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Method capable of simultaneously measuring organochlorine pesticide concentration and synthetic musk concentration in human serum

A technology for synthesizing musk and organochlorine, which is applied to measuring devices, instruments, scientific instruments, etc., to achieve high accuracy and stable repeatability

Inactive Publication Date: 2014-02-19
SHANGHAI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, there is no document reporting the method and data for simultaneously detecting organochlorine pesticides and synthetic musk in human serum. The health impact of

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  • Method capable of simultaneously measuring organochlorine pesticide concentration and synthetic musk concentration in human serum
  • Method capable of simultaneously measuring organochlorine pesticide concentration and synthetic musk concentration in human serum
  • Method capable of simultaneously measuring organochlorine pesticide concentration and synthetic musk concentration in human serum

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Embodiment

[0043] In this embodiment, the process and steps of the determination method are as follows:

[0044] A. Liquid-liquid extraction and constant weight fat

[0045] Measure 8ml of serum sample into a separatory funnel, add 0.005μg of recovery indicators TCMX+PCB209, D3-AHTN and D15-MX, then add 8ml 8% potassium oxalate and 10ml ethanol and shake well, then add 5ml The mixture is oscillated with ether, and the mixed solution is subjected to liquid-liquid extraction: first add 20ml of n-hexane, fully shake, stand for layering, separate the liquid after the liquid level is separated, take the supernatant and pour it into the chicken heart bottle, set aside ; Pour the collected lower layer turbid liquid into the separatory funnel, add 10ml of n-hexane, shake well, let stand for layering, take the supernatant liquid and pour it into the original ready-to-use chicken heart bottle; the lower layer turbid liquid according to the same method as above Repeat the extraction three times step by...

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Abstract

The invention relates to a method capable of simultaneously measuring organochlorine pesticide concentration and synthetic musk concentration in human serum. According to the method, the aim of measuring organochlorine pesticide and synthetic musk in the serum is realized through a chemical pretreatment method of liquid-liquid extraction, gel permeation chromatography and silica gel alumina column, in combination with a detection technology of gas chromatography-electron capture detection (GC-ECD) and gas chromatography-mass spectrometry (GC-MS). The method provided by the invention belongs to the technical field of physico-chemical analysis measurement of organic chemicals. In the method provided by the invention, the serum pretreatment process comprises organic solvent liquid-liquid extraction, gel permeation chromatographic column degreasing, silica gel alumina column purification, separation and concentrating to constant volume, finally, GC-ECD and GC-MS are used for detection, detection spectrograms of organochlorine pesticide and synthetic musk in serum sample are respectively obtained, and concentration values of organochlorine pesticide and synthetic musk in the serum are calculated through a special formula. The method provided by the invention is a steady, relatively high-sensitivity and high-precision effective measurement method.

Description

Technical field [0001] The invention relates to a stable and reproducible method for simultaneously detecting the concentration of organochlorine pesticides and synthetic musk in human serum. The chemical pretreatment method of liquid-liquid extraction-gel permeation chromatography column-silica gel alumina column is combined with gas chromatography Electron capture detection (GC-ECD) and gas chromatography-mass spectrometry (GC-MS) detection techniques are used to achieve the purpose of determining organochlorine pesticides and synthetic musk in serum. The invention belongs to the technical field of physical and chemical analysis and determination of organic chemical substances. Background technique [0002] Organochlorine pesticides (OCPs) belong to the first batch of 12 types of persistent organic pollutants for mandatory control stipulated in the international convention "Stockholm Convention on Persistent Organic Pollutants". Organochlorine pesticides have strong hydroph...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 张晓岚周静马莉徐青敬烨
Owner SHANGHAI UNIV
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