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Fingerprint spectrum detection method for aboveground part of ligusticum wallichii

A detection method and fingerprint technology, which is applied in the field of traditional Chinese medicine fingerprints, can solve problems such as difficult quality control of Rhizoma Chuanxiong aboveground parts, chemical composition properties, and great polarity differences, and achieve good consistency and similarity.

Inactive Publication Date: 2020-06-09
SICHUAN NEO GREEN PHARMA TECH DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The properties and polarities of various chemical components in the aerial parts of Chuanxiong are very different. It is difficult to reflect the whole picture of the existing single component detection, and it is difficult to carry out overall quality control on the aerial parts of Chuanxiong.
However, there is no research report on the fingerprints of the above-ground parts of Ligusticum chuanxiong

Method used

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  • Fingerprint spectrum detection method for aboveground part of ligusticum wallichii
  • Fingerprint spectrum detection method for aboveground part of ligusticum wallichii
  • Fingerprint spectrum detection method for aboveground part of ligusticum wallichii

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Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1 The fingerprint detection of Rhizoma Chuanxiong aerial part of the present invention

[0024] 1) Preparation of the test solution: Take 2.0 g of the sample powder of the aboveground part of Rhizoma Chuanxiong (passed through a No. 6 sieve), accurately weigh it, put it in a 50 mL conical flask with a stopper, add 10 mL of methanol accurately, weigh it, and ultrasonically treat it for 30 minutes , let it cool, make up for the weight loss, shake well, pass through a 0.22 μm microporous membrane, and take the subsequent filtrate to obtain;

[0025] 2) Preparation of reference substance solution

[0026] Take 10mg of ligustilide reference substance, weigh it accurately, add methanol to a 10mL volumetric flask, dissolve to the mark, and shake well to obtain a stock solution of ligustilide reference substance with a concentration of 1.0780mg / mL. Precisely measure 1.0mL of the stock solution, put it in a 5mL volumetric flask, add methanol to dilute to the mark, sh...

experiment example 1

[0034] 1 Instruments and reagents

[0035] 1.1 Instrument

[0036] HPLC-DAD3000 high-performance liquid chromatography (Thermo Fisher Scientific Co., Ltd.); chromatographic column KromasilC 18 (250×4.6mm, 5μm); FA 2004N electronic balance (Shanghai Minqiao Precision Scientific Instrument Co., Ltd.); UPS-11-20L ultrapure water machine (Sichuan Youpu Ultrapure Technology Co., Ltd.), KQ5200E ultrasonic cleaning Device (Kunshan Ultrasonic Instrument Co., Ltd.).

[0037] 1.2 Reagents and medicinal materials

[0038] Butenylphthalide (BP0061, Chengdu Prefa Technology Development Co., Ltd.); Ligustilide (DDYJ0412-08, Chengdu Dingdang Times Pharmaceutical Technology Co., Ltd.); Acetonitrile (chromatographically pure, SIGMA-ALORICH); Phosphoric acid (AR, Chengdu Kelong Chemicals Co., Ltd.); methanol (chromatographically pure, SIGMA-ALORICH); methanol (AR, Chengdu Kelong Chemicals Co., Ltd.); ultrapure water. 11 batches of aerial parts of Rhizoma Chuanxiong from different origins in...

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Abstract

The invention discloses a fingerprint spectrum detection method for an aboveground part of ligusticum wallichii. The fingerprint spectrum detection method for the aboveground part (stems and leaves) of the ligusticum wallichii fills the blank of quality detection of the aboveground parts of the ligusticum wallichii, and a standard quality control system is established for the aboveground part of the ligusticum wallichii. According to the fingerprint spectrum of the aboveground part of the ligusticum wallichii, 20 common peaks are calibrated, and two components, namely ligustilide and butenylphthalide, are identified. Compared with a control spectrum, the fingerprint spectrum similarity of the aboveground part of the ligusticum wallichii in different batches is 0.942-0.990, and the similarity is good such that the chemical components of the aboveground part of the ligusticum wallichii in different producing areas have good consistency, and the method can be used for controlling the quality of the aboveground part of the ligusticum wallichii.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicines, in particular to a method for detecting the fingerprints of the above-ground parts of Rhizoma Chuanxiong. Background technique [0002] Ligusticum chuanxiong is the dried rhizome of Lingusticum chuanxiong Hort., an Umbelliferae plant. It mainly contains volatile oil, alkaloids, phenolic acids, lactones, terpenes, phthalide derivatives, polysaccharides and inorganic elements. At present, the research and application of Rhizoma Chuanxiong are concentrated on the underground rhizomes, and the research and utilization of the aboveground parts of Rhizoma Chuanxiong are very few. The aerial parts of Chuanxiong are rich in resources, accounting for 75% of the fresh weight of the whole plant, but most of them are discarded, resulting in a great waste of resources. The aboveground parts of Chuanxiong can relieve wind, calm the liver, and dispel wind and cold in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N30/8686G01N2030/027
Inventor 彭成周厚成胡昌江谭玉柱闫洪玲
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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