Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for evaluating quality level through detection of contents of active ingredients with calcium-antagonistic function in Chinese angelica medicine material

A technology of quality grade and active ingredients, applied in the field of grade, can solve the problems of lack of effective combination, unable to fully reflect the quality attributes of Angelica sinensis, lack of detection methods, etc., and achieve the effect of rapid evaluation

Active Publication Date: 2019-07-26
NANKAI UNIV
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these indicators have a certain degree of control on the quality of Angelica sinensis, they cannot fully reflect the specific quality attributes of Angelica sinensis.
At present, there is no effective combination of the evaluation of the quality of Angelica sinensis and the analysis of its medicinal efficacy, and there is a lack of fast, simple and easy detection methods.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for evaluating quality level through detection of contents of active ingredients with calcium-antagonistic function in Chinese angelica medicine material
  • Method for evaluating quality level through detection of contents of active ingredients with calcium-antagonistic function in Chinese angelica medicine material
  • Method for evaluating quality level through detection of contents of active ingredients with calcium-antagonistic function in Chinese angelica medicine material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] UPLC-Q / Tof is used for the identification of the active ingredients of Angelica sinensis, and when the method of the present invention is used for actual operation, the content of the active ingredients of Angelica sinensis can be determined only by using UPLC.

[0030] 1) Preparation of angelica medicinal material methanol extract:

[0031] Take a dried sample of Angelica sinensis, crush it and filter it through a 100-mesh No. 6 sieve to obtain Angelica sinensis powder, take 0.5g of the powder, add 50mL of 70% methanol aqueous solution, and ultrasonically extract at 600W for 30 minutes at room temperature, 4000 rpm, centrifuge for 10 minutes, remove the residue, take the supernatant, filter through a 0.22 μm microporous membrane, and store the filtrate at 4°C;

[0032] 2) Ultra-high performance liquid chromatography conditions:

[0033] ColumnACQUITY BEHC18 chromatographic column (2.1×100mm, 1.7μm); flow rate 0.4mL / min; PDA detector, detection wavelength 190-400nm; ...

Embodiment 2

[0045] Example 2. Based on Ca 2+ UPLC-Q / Tof spectral efficiency screening of active ingredients of Angelica sinensis with antagonistic activity

[0046] (1) Cell culture: Take 293T human kidney epithelial cells (purchased from Type Culture Collection, RockvilleMD, USA), add 4mL DMEM high-glucose complete medium, and place at 25cm 2 Cell culture flasks at 37 °C, 5% CO 2 cultured in an incubator. Cell transfection was carried out when the confluence of cell growth reached 70%.

[0047] DMEM high-glucose complete medium: add fetal bovine serum by 10% in DMEM high-glucose medium (Gibco, USA), add ampicillin (Gibco, USA) by 1%, add streptomycin (Gibco, USA) by 1% .

[0048] (2) Construction of high-expression plasmid strains: Thaw competent cells Escherichia coli DH5α (Ecoli DH5α) in an ice-water bath, and add target DNA (Ca 2+ Luciferase reporter PGL4.30 plasmid and internal reference luciferase reporter gene plasmid Renilla) Dip the bottom of the tube with your fingers, mix ...

Embodiment 3

[0056] Example 3. Ca 2+ Antagonism verification

[0057] With reference to the method of Example 1, sieve the pulverized Angelica medicinal material, take 0.5g of the powder, add 50mL volume concentration of 70% methanol aqueous solution, and ultrasonically extract for 30 minutes at a power of 600W at room temperature, and centrifuge at 4000 rpm for 10 minutes. Minutes, remove the residue, collect the supernatant, concentrate under reduced pressure and freeze-dry to obtain dry powder of the extract.

[0058] Accurately weigh the above-mentioned freeze-dried powder (containing Z-ligustilide: 13.832mg / g and angelica lactone A: 1.476mg / g), and use dimethyl sulfoxide (DMSO) as solvent to prepare a concentration of 10 -1 、10 -2 、10 -3 kg / L solution, stored at 4°C for future use. At the same time, we accurately weighed an appropriate amount of Z-ligustilide (commercial product) and angelica lactone A (commercial product), and dissolved it in a small amount of DMSO to prepare a c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for evaluating the quality level through detection of the contents of active ingredients with a calcium-antagonistic function in a Chinese angelica medicine material.The method includes steps: (1) detecting contents of Z-ligustilide and levistilide A in the Chinese angelica medicine material through an ultra-performance liquid chromatography, wherein the mg numberof Z-ligustilide in 1g of the Chinese angelica medicine material is expressed by X1, and the mg number of levistilide A in 1g of the Chinese angelica medicine material is expressed by X2; and (2) enabling the relation between the intracellular calcium-antagonistic function and Z-ligustilide and levistilide A to satisfy the following function: Y=(31.2579X1+381.352X2-248.979X1X2+18.4822)*100%, wherein Y is an evaluation value of the intracellular calcium-antagonistic function in the Chinese angelica medicine material for short, the range interval of Y is 0-100%, and the Chinese angelica medicine material is divided into seven quality levels from level 1 to level 7. According to the method, the quality level of Chinese angelica can be simply and rapidly evaluated.

Description

technical field [0001] The invention belongs to the technical field of quality evaluation of Chinese medicinal materials, and relates to a technical method for quickly evaluating the quality grade of angelica. Background technique [0002] Angelica sinensis is the dried root of Angelica sinensis (Oliv.) Diels, a plant of the Umbelliferae family. It has the effects of nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, and moisturizing the intestines. Angelica is native to the damp areas with high altitude in western China, and wild angelica is distributed in Minxian County, Gansu and Tibet. The angelica currently circulating in the market are all cultivated varieties, mainly from Gansu, Yunnan, Hubei, Sichuan and other provinces. Due to the influence of temperature, soil and planting methods, the traits and quality of angelica are different in different regions. Therefore, more convenient and reliable methods are needed for the qual...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/02
CPCG01N30/02G01N30/88G01N2030/027G01N2030/8809
Inventor 白钢姜民侯媛媛闫孟琳丁国钰丛龙飞高洁牛瑞雪
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com