44 results about "Hematinic" patented technology

The invention belongs to the technical field of gene engineering, and particularly relates to fusion vitamin D hydroxylase and application thereof in preparation of 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3. The fusion vitamin D hydroxylase comprises a Vdh-K1 heme structural domain of which the amino acid sequence is shown as SEQ ID NO.1, a CYP116B65 redox structural domain of which the amino acid sequence is shown as SEQ ID NO.3, and a linker for connecting the Vdh-K1 heme structural domain and the CYP116B65 redox structural domain. The fusion vitamin D hydroxylase is a self-sufficiency enzyme, and can realize stable catalytic conversion of vitamin D2 and vitamin D3 on the premise of not depending on an Fdx and Fdr assisted electron transfer system.

The invention discloses a food therapy height increasing method. The food therapy height increasing method comprises the following raw materials: enzymolysis bone calcium, oyster peptide, vitamin C, beta-carotene and hemin chloride. According to the present invention, the components such as the enzymolysis bone calcium, the oyster peptide, the vitamin C, the beta-carotene, the heme chloride and the like, which are beneficial to bone repair and bone growth and development, are extracted so as to meet the bone growth requirements of teenagers and children; the raw materials are processed and prepared into powder, all the components are prepared into the solid beverage in a mixing mode, and the solid beverage can be taken by adding sugar or honey or other flavors into water when being eaten. In conclusion, the solid beverage is suitable for teenagers and children to eat, different from a traditional food therapy mode, various growth promoting requirements are met, cooking is not needed, and the solid beverage can be taken after being mixed with water and is convenient to eat.

The invention relates to the technical field of heme purification, in particular to a heme extraction and purification method. Comprising the following steps: taking fresh animal blood, centrifugally collecting lower red blood cell precipitate, adding normal saline, uniformly mixing and washing; adding an ethanol solution with the concentration of 1-10%, uniformly mixing, and ultrasonically crushing for 15-60 seconds under the power of 300-800W to obtain erythrocyte crushing liquid; adding an acidic acetone solution into the erythrocyte crushing liquid, uniformly mixing, extracting at normal temperature, centrifuging, and collecting supernate to obtain an acidic acetone extracting solution; slowly adding a NaOH solution to adjust the pH value; adding sodium acetate, stirring, standing, taking precipitate, washing and drying to obtain a crude product; filling a silica gel column; loading the crude product, eluting with an eluent, and collecting an elution component containing a target product; the eluent comprises dichloromethane, methanol and formic acid; and removing the mobile phase in the elution component. The method has the advantages of simple process, low cost, environmental friendliness and capability of obtaining a high-purity product.

The present application belongs to the field of biological medicine and molecular biology technology, and particularly relates to a marker for predicting the occurrence of repeated implantation failure and application thereof. The present application finds that abnormal lipid peroxidation in uterine epithelium can cause implantation defects, and the lipid peroxidation level of the uterus in patients with repeated implantation failure or repeated spontaneous abortion is enhanced, wherein the content of active aldehyde substance MDA and non-heme iron in serum can be used as a new non-invasive clinical index for predicting RIF patients; further, eliminating uterine lipid ROS to improve endometrial receptivity can be used as a potential new treatment strategy to improve embryo implantation and clinical pregnancy rate, and therefore has good practical application value.

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. The preparation method includes the following steps: S1, dissolving β-alanyl-L-histidine in ultrapure water and stirring until homogeneous to obtain solution A; S2, dissolving heme chloride in dimethyl sulfoxide and stirring until homogeneous to obtain solution B; S3, mixing solution A and solution B until homogeneous, adjusting the pH, heating in a water bath, incubating at room temperature, centrifuging, discarding the precipitate, and retaining the supernatant; S4, lyophilizing and grinding the supernatant obtained in S3 to obtain the self-assembled supramolecular peptide nanozyme material. This invention discloses a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. This supramolecular peptide nanozyme, when applied to food quality and safety testing, can achieve visual and sensitive colorimetric detection of biogenic amines in spoiled food.

The application relates to the technical field of meat processing, in particular to a vacuum circulation pickling method for meat. The method comprises the following steps: submerging meat pieces to be pickled in pickling liquid added with 0.1% hematin iron; first, applying a transient high-voltage electromagnetic pulse to precisely perforate the cell membrane of the meat; then, applying an alternating vortex magnetic field with a specific frequency; utilizing the high-frequency micro-vibration of the hematin iron in the magnetic field to generate a magnetohydrodynamic effect, expand the micro-pore channel and pump the pickling material molecules into the cell interior; finally, combining vacuum circulation alternating treatment to further press the pickling material into the tissue gap. The added hematin iron significantly enhances the promotion effect of the magnetic field on the pickling material delivery, and the pickling time is shortened to 30 minutes or less. It is proved through measurement that the pickling liquid absorption rate of the process is high, the penetration reaches the geometric center of the meat pieces, and the integrity and water holding capacity of the meat structure are perfectly preserved.

The application belongs to the technical field of drug screening, and particularly relates to a method for applying oxalic acid to high-throughput screening of drugs for preventing and treating anemia related to abnormal differentiation of erythroid cells. The application provides application of oxalic acid in screening of drugs for preventing and treating anemia related to abnormal differentiation of erythroid cells and a high-throughput drug screening method. The application uses oxalic acid to detect hematin, has the characteristics of high sensitivity and high accuracy, can be used to construct a high-throughput drug screening output system, significantly reduces the cost of drug screening, and improves the screening efficiency and accuracy.

The application discloses a hemoglobin mutant with improved stability and application thereof, and belongs to the technical field of genetic engineering. The application lays a foundation for obtaining an ideal stable human hemoglobin mutant by designing a high-throughput screening method for the structural stability of human hemoglobin and the hematin binding intensification mutant. The application determines hot amino acid sites for the structural stability and thermal stability modification of human hemoglobin through semi-rational design, and further carries out iterative saturation mutation to obtain an ideal mutant rHb 9th The application has the advantages that in terms of structural stability, thermal stability, anti-autoxidation capacity, hematin binding rate, anti-hematin loss rate and human hemoglobin yield, etc. wild‑type The application has the advantages that in terms of structural stability, thermal stability, anti-autoxidation capacity, hematin binding rate, anti-hematin loss rate and human hemoglobin yield, etc.

The application provides a low-temperature vacuum-multicavity evaporation linkage heme iron preparation method and device, relates to the technical field of biological medicine and functional food deep processing, and solves the problems of local overheating and fouling caused by traditional wall heat transfer.

The invention discloses a heme preparation method adopting an enzyme method, and relates to the technical field of biological preparation, and the heme preparation method comprises the following steps: S1, taking animal blood and blood powder, carrying out anticoagulation treatment and centrifugal separation to obtain red blood cells, and carrying out cell breaking treatment on the red blood cells to release hemoglobin to obtain a hemoglobin solution; s2, mixing the obtained hemoglobin solution with protease, and carrying out enzymolysis reaction for 1-5 hours under the conditions that the pH value is 3.0-5.0 and the temperature is 20-40 DEG C, so that hemoglobin is subjected to enzymolysis to release heme, and an enzymolysis reaction solution is obtained; s3, precipitating, centrifuging, washing and drying the obtained enzymolysis reaction liquid to obtain a heme product. According to the preparation method of the heme, key links such as raw material pretreatment, enzymolysis reaction and product separation and purification are finely regulated and controlled, so that the heme preparation efficiency and the product quality are remarkably improved, the erythrocyte separation effect is effectively improved, and the release efficiency and the enzymolysis stability of the heme are enhanced.

To provide alternative protein-rich food products that are not derived from animals, but are free of unhealthy meat-related components such as saturated fatty acids and cholesterol, are free of the deleterious environmental effects of animal husbandry, and provide similar texture and flavor characteristics and functional properties as animal meat.SOLUTION: Structured food compositions, such as meat substitute compositions, comprising microbial-derived protein products, such as microbial-derived protein hydrolysates, are provided. Methods of making such products are also provided. Also provided are flavor potentiator compositions comprising heme-containing polypeptides, such as microbial-derived flavohemoglobin, and food compositions, such as meat substitute compositions, comprising the heme-containing flavor potentiator compositions. In one aspect, the flavor potentiator comprises a heme-containing polypeptide from a hydrogen-oxidizing microorganism, wherein (i) the hydrogen-oxidizing microorganism is a Cupriavidus microorganism or (ii) the hydrogen-oxidizing microorganism grows under microaerobic conditions.SELECTED DRAWING: None

This invention belongs to the field of genetic engineering technology, specifically relating to a fusion vitamin D hydroxylase and its application in the preparation of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3. The fusion vitamin D hydroxylase comprises a Vdh-K1 heme domain (as shown in SEQ ID NO.1) and a CYP116B65 redox domain (as shown in SEQ ID NO.3), as well as a linker connecting the two. This fusion vitamin D hydroxylase is a self-sufficient enzyme that can stably catalyze the conversion of vitamin D2 and vitamin D3 without relying on an Fdx and Fdr-assisted electron transport system.

This invention relates to the technical field of pet food, and more particularly to a functional pet nutritional supplement and pet nutritional food and its preparation method, comprising 10%–15% beef liver chunks, 20%–30% chicken breast strips, 35%–45% a mixture of cooked rice flour / corn / rice, 0.3%–1.0% chicken oil, 0.3%–1.0% fish oil, 2%–5% pumpkin puree, 1%–3% chopped carrots, 3%–7% egg yolk powder, 3%–7% a mixture of traditional Chinese medicine extracts, 0.5%–1.5% amino acid and mineral premix, 0.5%–1.5% vitamin and antioxidant premix, 0.0001%–0.001% probiotic mixture, 0.05%–0.3% potassium sorbate, 0.05%–0.3% sodium benzoate, 0.05%–0.2% citric acid, and BHA / BHT. 0.05%–0.2%, with the remainder being chicken broth. Beef liver is rich in heme iron, which, combined with lecithin in egg yolk powder, promotes iron absorption. Traditional Chinese medicine extracts significantly reduce IL-6, IgG, and IgM levels, regulating hyperimmune states. Meanwhile, ingredients such as astragalus enhance normal immunity, achieving a balance of "strengthening the body's resistance and eliminating pathogens."

The present application relates to a kind of intestinal absorption hemin, belong to biological medicine technical field, the intestinal absorption hemin, including hemin and gamma-cyclodextrin;The molar ratio of hemin and cyclodextrin is 1:2;The preparation method of the intestinal absorption hemin, comprising the following steps: (1) hemin is mixed with ammonia water, to obtain hemin solution;Cyclodextrin is mixed with water, to obtain cyclodextrin aqueous solution;(2) the hemin solution is mixed with the cyclodextrin aqueous solution, to obtain mixed solution;(3) the mixed solution is stirred, and dried for 5h, to obtain intestinal absorption hemin.The intestinal absorption hemin prepared in the present application has the advantages of water solubility and stability, high bioavailability after gastrointestinal digestion, and can be used as a raw material for preparing nutritional fortifier for iron supplementation.

The present invention discloses a heme iron – glycine chelated iron supplement preparation, belonging to the technical field of nutritional and pharmaceutical preparations. The preparation employs a four-source composite of heme iron, glycine chelated iron, ferrous gluconate, and ferrous lactate, enabling iron supplementation via heme receptor absorption, amino acid chelation pathways, and ionic diffusion. This design overcomes the limitations of single iron sources, including gastrointestinal irritation and low absorption of inorganic iron, dietary inhibition of conventional organic iron, and the high cost or slow onset of novel iron sources. The four iron sources act synergistically to reduce free iron ions and integrate multiple absorption pathways. As a result, gastrointestinal irritation is minimized, and the inhibitory effects of phytic acid, oxalic acid, and tannins are reduced, enabling stable and efficient intestinal absorption. The preparation achieves non-irritating, highly bioavailable, and sustained iron supplementation.

The application discloses a red blood cell drug delivery system of a modified indocyanine green derivative dye, the drug delivery system is loaded with a photosensitizer IR808 on the surface of red blood cells and is wrapped with hematin chloride in the interior of the red blood cells. The application also discloses a use of the near-infrared light response red blood cell drug delivery system of the modified indocyanine green derivative dye in preparation of a drug for treating periodontitis. The drug delivery system can be used for PDT anti-infection treatment in the assistance of NIR laser according to the infection condition of a clinical periodontal disease, and can promote the release of hemin and eliminate potential intracellular bacterial infection. The drug delivery system can be used for intrabag administration in a periodontal pocket according to the clinical requirement of different stages of periodontitis, so that a patient can be free from the trauma of periodontal surgery.

The application discloses a kind of DOC-Chaps synergistic core's clinical and animal tissue rapid transparent reagent and three-dimensional imaging method. Rapid transparent reagent is mainly dissolved in deionized water by sodium deoxycholate (DOC), 3-[(3-cholamide propyl) dimethylamino]-1-propane sulfonic acid (Chaps), urea and 1-methylimidazole; DOC efficiently dissolves high-density lipid, Chaps gently destroys membrane structure, both complement each other, realize lipid rapid, depth clearance and significantly reduce light scattering; urea loosens collagen fiber and protein aggregate, improves the penetration rate of reagent; 1-methylimidazole selectively removes hemoglobin, reduces light absorption. The transparent reagent of the application can make millimeter thick brain slices in 4 (mouse brain) / 6 (human brain) hours The light transmittance of specific wavelength is greater than or equal to 90%, and there is no obvious tissue shrinkage / expansion, the compatibility of fluorescent protein and antibody label is excellent, suitable for three-dimensional imaging of high-lipid, high-hemoglobin samples such as in-vitro human brain, kidney, liver and mouse brain, heart, kidney, liver, intestinal tract, embryo, etc.

The invention discloses ruddy heme iron red ginseng and donkey-hide gelatin nutritional milk powder and a preparation method thereof, and belongs to the technical field of milk powder, the ruddy heme iron red ginseng and donkey-hide gelatin nutritional milk powder comprises the following components in parts by mass: a wet process part: 420-455 parts of whole milk powder, 306-345 parts of dried skim milk, 185-205 parts of isomaltooligosaccharide (type 50) powder, 35-40 parts of polydextrose, 11.5-14.5 parts of a milk powder nutrition enhancer III, 0.3-0.5 part of compound L-carnitine, and 0.1-0.3 part of heme chloride; according to the ruddy heme iron red ginseng and donkey-hide gelatin nutritional milk powder and the preparation method thereof, the heme chloride is adopted as an iron source, the bioavailability of the red heme iron red ginseng and donkey-hide gelatin nutritional milk powder is remarkably higher than that of ferrous sulfate used in traditional iron-supplementing milk powder, meanwhile, through beta-cyclodextrin embedding treatment, the fishy smell problem of an iron agent is solved, gastrointestinal tract stimulation is avoided, and L-sodium ascorbate is matched, so that the nutrition of the milk powder is improved. And the iron absorption rate can be further improved.

The invention relates to the technical field of intestinal microecology, and particularly discloses an in-vitro fermentation system for rapid targeted screening, regulation and control of intestinal TMA generation, an establishment method and application, and the in-vitro fermentation system comprises the following components in parts by weight: 2-3 parts of yeast extract, 0.8-1.2 parts of L-cysteine, 1.5-2.5 parts of heme, 0.8-1.0 part of sodium chloride, 0.008-0.012 part of calcium chloride hexahydrate and 0.4-0.5 part of dipotassium phosphate. The feed is prepared from the following raw materials in parts by weight: 0.08 to 0.1 part of magnesium sulfate heptahydrate, 0.15 to 0.25 part of vitamin mixture, 7 to 9 parts of starch, 0.01 to 0.8 part of choline and 0.8 to 1.2 parts of resazurin. The method has the characteristics that the large-scale and high-flux active substance screening requirement can be met, and the TMA generation and related substrate metabolism quantitative control can be realized.

The application belongs to the technical field of hematin quantitative detection, and specifically discloses application of methylene blue cyclopropyl formylated derivative as a chromogenic substrate for detecting hematin, and uses methylene blue cyclopropyl formylated derivative AMB as a chromogenic substrate for detecting hematin Hemin; compared with a classical substrate TMB, the methylene blue cyclopropyl formylated derivative AMB of the application has higher sensitivity in the detection of hematin Hemin; in addition, the amino protons of the methylene blue cyclopropyl formylated derivative AMB of the application are fully substituted by methyl, which inhibits the participation of active oxygen free radicals in oxidation, but is still sensitive to the oxidation pathway of ferric free radicals, has better selectivity compared with TMB, and the AMB system can effectively detect hematin Hemin in a serum sample. Therefore, it can be said that for the detection of hematin Hemin, AMB is a more excellent colorimetric substrate than TMB.

The application provides an iron supplement tabletting candy and a preparation method thereof, and the iron supplement tabletting candy comprises the following components in parts by weight: sorbitol 48-53 parts, hemoglobin peptide 35-40 parts, needle cherry fruit powder 4.1-4.8 parts, hemin chloride 1.2-1.5 parts, L-ascorbic acid 1.5-3.0 parts, and magnesium stearate 3.4-3.8 parts. The tabletting candy and the preparation method improve the viscosity of the mixture by using hemoglobin peptide in the preparation of the hemin tabletting candy, and the flowability and compressibility of the material can be improved by adding the flow agent powder in batches and premixing the material except part of the magnesium stearate and the hemoglobin peptide.

This invention provides a method for treating anemia in subjects with myelofibrosis. [Solution] A method is provided for treating anemia in a subject with myelofibrosis, comprising administering an effective amount of a hemoduvelin-induced BMP signaling antagonist, a hepcidin antagonist, to the subject.

The invention discloses a special compound premix feed for green-eggshell laying hens in an egg producing period and a preparation method of the special compound premix feed. The compound premix feed provided by the invention comprises green tea, nucleotide residues, calcium hydrophosphate, mountain flour, soybean lecithin oil, sodium chloride, heme protein powder, taurine, choline chloride, turmeric, an enzyme preparation, phytase, oregano oil, DL-methionine, an amino acid compound package, a trace element premix, an organic mineral compound package, a vitamin premix, vitamin C, vitamin E and an antioxidant. The components are screened from a large number of existing additive components and combined according to a specific proportion, and the obtained composition can improve the anti-stress capacity of chicken flocks, improve the stability of the groups and solve the problem that the groups are prone to being fried; meanwhile, aging of the laying hens can be delayed, and the production performance and egg quality of the green-shell laying hens in the egg producing period are improved, so that the eggshell color is improved for a long time, and the product market value is increased.

The invention relates to the technical field of biological medicine, in particular to an ovary protection oral liquid containing heme iron composite peptide and a preparation method and application thereof. The oral liquid comprises the following core components: 0.05-0.2 part of heme iron, 1.0-4.0 parts of peptide components, and preferably 0.5-2 parts of natto powder, it is found and proved for the first time that the heme iron and the specific peptide components have a synergistic effect on improvement of the ovarian function, ovarian oxidative damage can be remarkably reduced, the ovarian hypoxia state can be improved, and the oral liquid can be used for treating ovarian diseases. The ovarian blood perfusion amount and the uterine ovarian artery blood flow speed are increased. Meanwhile, the natto powder and the carob bean extract are added to generate a dual synergistic effect with the core component, the stability and bioavailability of the active components are ensured through an optimized preparation process, and animal experiments prove that the oral liquid can comprehensively and effectively protect the ovarian function and improve the ovarian function. The invention provides a safe and effective solution for solving the problems of single effect and unobvious synergistic effect of oral products in the prior art.

The invention provides a nanoreactor with an oxidation-immunization-blood vessel triple synergistic effect and a preparation method and application thereof.The nanoreactor is prepared by mixing a BSA solution, a heme solution and deionized water to obtain a nano-enzyme solution, then dropping a bFGF solution into the nano-enzyme solution through an injection pump, and conducting self-assembly of an NZs at bFGF dispersoid. And under an anaerobic condition, injecting a MnCl2 solution into the NZs (at) bFGF dispersion, immediately adding a Na2S solution, mixing, and removing redundant ions and by-products by using a dialysis bag to obtain the required nanoreactor (NZs (at) bFGF (at) MnS). Through an in-situ injection mode, the micro-environment of the diabetic wound surface is adjusted, immunoregulation and angiogenesis are promoted, and the purpose of promoting the repair of the diabetic wound surface is achieved.

This invention provides a method and apparatus for extracting heme iron based on a dual-membrane cascade and oxygen isolation protection, belonging to the field of biochemical separation and purification technology. The method first constructs a pneumatically suspended hemolytic environment, utilizing a metal sintered microporous distributor to generate a micron-sized inert bubble fluidized bed, achieving red blood cell disruption and deep deoxygenation under zero mechanical shear conditions, solving the molecular damage and oxidation problems caused by traditional processes. Then, a gas-liquid co-flow ultrafiltration mechanism is employed, introducing pulsed plug flow drive to physically peel off the protein gel layer on the membrane surface online, achieving dynamic anti-clogging and long-term stability in the macromolecular separation process. Finally, transmembrane partial pressure extraction is performed based on a reverse gas-scan nanofiltration model, distinguishing the migration paths of water molecules and dissolved oxygen, outputting a highly active concentrate and a closed-loop circulating gas flow. This invention effectively improves the extraction efficiency of the product Fe. 2+ Retention rate, purity, and continuity of industrial production.

The present invention relates generally to a stable liquid formulation of purified hemopexin comprising: (a) a hemopexin content of at least 50 mg / mL; (b) at least 15 mM phosphate buffer; (c) a pH from 5.8 to 8; and (d) at least 50 mM sodium chloride; and uses thereof.

A method for improving skin condition and / or relieving anemia of a subject in need thereof includes administering to the subject an effective amount of peach extract, where the peach extract is extracted from red flesh of peaches. The peach extract has at least one of the following effects of: resisting blue light-induced injury, improving oxidation resistance of skin fibrocytes, improving activity of mitochondria, promoting production of skin elastin, improving skin texture, reducing skin wrinkles, minimizing skin trichopores, improving the content of heme, and improving the content of iron in blood.

The application provides a hematin purification method, comprising the following steps: S1, centrifuging anticoagulant pig blood, collecting blood corpuscles after solid-liquid separation; S2, adding water in a proportion of 1g:2-4ml, mixing and adjusting pH to 8.5-8.8, adding 0.04-0.06% ascorbic acid, and heating to 55-65 DEG C, and adding 0.8-2.5% compound enzyme for enzymolysis for 6.5-8.0h; S3, adjusting the pH of the enzymolysis solution to 3.6, standing for 20-60min, centrifuging and collecting the precipitate; S4, using a magnetic resin column for column adsorption; S5, filtering after vacuum extraction and recovery of ethanol, adjusting pH to 5.0, centrifuging and collecting the precipitate; and S6, drying to obtain the product. Compared with the acid acetone method, the hematin purification method provided by the application greatly reduces the use of organic solvents and the product purity reaches 99%, which preliminarily meets the quality requirements of pharmaceutical raw materials.

This invention relates to the field of heme purification technology, and more particularly to a method for extracting and purifying heme chloride. The method includes: taking fresh animal blood, centrifuging to collect the lower red blood cell precipitate, adding physiological saline, mixing, and washing; adding a 1-10% ethanol solution, mixing, and ultrasonically disrupting at 300-800W for 15-60 seconds to obtain a red blood cell lysate; adding an acidic acetone solution to the red blood cell lysate, mixing, and extracting at room temperature, centrifuging to collect the supernatant to obtain an acidic acetone extract; slowly adding NaOH solution to adjust the pH; adding sodium acetate, stirring, allowing to stand, collecting the precipitate, washing, and drying to obtain a crude product; packing a silica gel column; loading the crude product onto an eluent, eluting with an eluent and collecting the eluent fraction containing the target product; the eluent includes dichloromethane, methanol, and formic acid; and removing the mobile phase from the eluent fraction. The advantages are: simple process, low cost, environmentally friendly, and the ability to obtain a high-purity product.