80 results about "Hematinic" patented technology

The invention belongs to the technical field of gene engineering, and particularly relates to fusion vitamin D hydroxylase and application thereof in preparation of 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3. The fusion vitamin D hydroxylase comprises a Vdh-K1 heme structural domain of which the amino acid sequence is shown as SEQ ID NO.1, a CYP116B65 redox structural domain of which the amino acid sequence is shown as SEQ ID NO.3, and a linker for connecting the Vdh-K1 heme structural domain and the CYP116B65 redox structural domain. The fusion vitamin D hydroxylase is a self-sufficiency enzyme, and can realize stable catalytic conversion of vitamin D2 and vitamin D3 on the premise of not depending on an Fdx and Fdr assisted electron transfer system.

The invention relates to a hydrogel dressing based on near-infrared light controllable cascade reaction and a preparation method thereof, and belongs to the technical field of hydrogel dressings. The preparation method of the hydrogel dressing based on the near-infrared light controllable cascade reaction comprises the following steps: respectively grafting carboxymethyl-beta-cyclodextrin and L-arginine to carboxymethyl chitosan by adopting an EDC / NHS crosslinking method to prepare cyclodextrin grafted chitosan and L-Arg grafted chitosan; adding a photosensitizer into the cyclodextrin grafted chitosan solution I to obtain a C-C-I inclusion compound solution; adding hemin into the cyclodextrin grafted chitosan solution II to obtain a C-C-H inclusion compound solution; and mixing the oxidized hyaluronic acid solution, the L-Arg grafted chitosan solution, the C-C-I clathrate compound solution and the C-C-H clathrate compound solution. The CLIHO disclosed by the invention can be used for synergistically resisting bacteria through PTT / NO and synchronously adjusting a wound microenvironment, so that the healing speed and the healing quality of infectious chronic wounds are improved.

The invention discloses a food therapy height increasing method. The food therapy height increasing method comprises the following raw materials: enzymolysis bone calcium, oyster peptide, vitamin C, beta-carotene and hemin chloride. According to the present invention, the components such as the enzymolysis bone calcium, the oyster peptide, the vitamin C, the beta-carotene, the heme chloride and the like, which are beneficial to bone repair and bone growth and development, are extracted so as to meet the bone growth requirements of teenagers and children; the raw materials are processed and prepared into powder, all the components are prepared into the solid beverage in a mixing mode, and the solid beverage can be taken by adding sugar or honey or other flavors into water when being eaten. In conclusion, the solid beverage is suitable for teenagers and children to eat, different from a traditional food therapy mode, various growth promoting requirements are met, cooking is not needed, and the solid beverage can be taken after being mixed with water and is convenient to eat.

The invention relates to the technical field of heme purification, in particular to a heme extraction and purification method. Comprising the following steps: taking fresh animal blood, centrifugally collecting lower red blood cell precipitate, adding normal saline, uniformly mixing and washing; adding an ethanol solution with the concentration of 1-10%, uniformly mixing, and ultrasonically crushing for 15-60 seconds under the power of 300-800W to obtain erythrocyte crushing liquid; adding an acidic acetone solution into the erythrocyte crushing liquid, uniformly mixing, extracting at normal temperature, centrifuging, and collecting supernate to obtain an acidic acetone extracting solution; slowly adding a NaOH solution to adjust the pH value; adding sodium acetate, stirring, standing, taking precipitate, washing and drying to obtain a crude product; filling a silica gel column; loading the crude product, eluting with an eluent, and collecting an elution component containing a target product; the eluent comprises dichloromethane, methanol and formic acid; and removing the mobile phase in the elution component. The method has the advantages of simple process, low cost, environmental friendliness and capability of obtaining a high-purity product.

The present application belongs to the field of biological medicine and molecular biology technology, and particularly relates to a marker for predicting the occurrence of repeated implantation failure and application thereof. The present application finds that abnormal lipid peroxidation in uterine epithelium can cause implantation defects, and the lipid peroxidation level of the uterus in patients with repeated implantation failure or repeated spontaneous abortion is enhanced, wherein the content of active aldehyde substance MDA and non-heme iron in serum can be used as a new non-invasive clinical index for predicting RIF patients; further, eliminating uterine lipid ROS to improve endometrial receptivity can be used as a potential new treatment strategy to improve embryo implantation and clinical pregnancy rate, and therefore has good practical application value.

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. The preparation method includes the following steps: S1, dissolving β-alanyl-L-histidine in ultrapure water and stirring until homogeneous to obtain solution A; S2, dissolving heme chloride in dimethyl sulfoxide and stirring until homogeneous to obtain solution B; S3, mixing solution A and solution B until homogeneous, adjusting the pH, heating in a water bath, incubating at room temperature, centrifuging, discarding the precipitate, and retaining the supernatant; S4, lyophilizing and grinding the supernatant obtained in S3 to obtain the self-assembled supramolecular peptide nanozyme material. This invention discloses a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. This supramolecular peptide nanozyme, when applied to food quality and safety testing, can achieve visual and sensitive colorimetric detection of biogenic amines in spoiled food.

The invention relates to the technical field of heme extraction, and particularly discloses a method for extracting heme from livestock and poultry blood cells. The method comprises the following steps: pretreating fresh livestock and poultry blood, drying to obtain livestock and poultry blood cell powder, mixing tetrabutylammonium bromide and polypropylene glycol according to a molar ratio of 1: 2-1: 8 to obtain a heme extracting agent, adding the heme extracting agent into the livestock and poultry blood cell powder, mixing the livestock and poultry blood cell powder and the heme extracting agent according to a ratio of 1: 10-1: 100 g / mL, carrying out ultrasonic-assisted extraction, and carrying out freeze-drying to obtain the heme extract. And centrifugally separating and taking supernate to obtain a heme extracting solution, and purifying the heme extracting solution through a chromatographic column to obtain the heme with higher purity. The extraction method is simple, the steps are easy to operate, and compared with a traditional organic solvent for extracting the heme, the extraction agent dosage is small, the extraction time is shortened, the heme extraction rate is high, and the heme purity is good.

The application relates to the technical field of meat processing, in particular to a vacuum circulation pickling method for meat. The method comprises the following steps: submerging meat pieces to be pickled in pickling liquid added with 0.1% hematin iron; first, applying a transient high-voltage electromagnetic pulse to precisely perforate the cell membrane of the meat; then, applying an alternating vortex magnetic field with a specific frequency; utilizing the high-frequency micro-vibration of the hematin iron in the magnetic field to generate a magnetohydrodynamic effect, expand the micro-pore channel and pump the pickling material molecules into the cell interior; finally, combining vacuum circulation alternating treatment to further press the pickling material into the tissue gap. The added hematin iron significantly enhances the promotion effect of the magnetic field on the pickling material delivery, and the pickling time is shortened to 30 minutes or less. It is proved through measurement that the pickling liquid absorption rate of the process is high, the penetration reaches the geometric center of the meat pieces, and the integrity and water holding capacity of the meat structure are perfectly preserved.

The invention belongs to the technical field of nano-enzyme materials, and discloses a cucurbituril nano-enzyme for dispelling effects of alcohol and protecting liver and a preparation method thereof. The antialcoholism and liver-protecting cucurbituril nano-enzyme comprises cucurbituril, heme iron and hydrated iron nano-particles. The cucurbituril nano-enzyme capable of dispelling effects of alcohol and protecting liver keeps constant catalytic efficiency in a pH range of 4.0-8.8, is resistant to high and low temperatures, does not inactivate, can complete ethanol decomposition in the stomach, and has the effect of protecting liver.

The application belongs to the technical field of drug screening, and particularly relates to a method for applying oxalic acid to high-throughput screening of drugs for preventing and treating anemia related to abnormal differentiation of erythroid cells. The application provides application of oxalic acid in screening of drugs for preventing and treating anemia related to abnormal differentiation of erythroid cells and a high-throughput drug screening method. The application uses oxalic acid to detect hematin, has the characteristics of high sensitivity and high accuracy, can be used to construct a high-throughput drug screening output system, significantly reduces the cost of drug screening, and improves the screening efficiency and accuracy.

The invention discloses a nano-enzyme antibiotic adjuvant as well as a preparation method and application thereof, and belongs to the technical field of nano-drug delivery system antibiosis. The method comprises the following steps: dispersing bovine serum albumin and heme in water, and carrying out ultrasonic oscillation; injecting absolute ethyl alcohol, adding glutaraldehyde, continuing the reaction, centrifuging after the reaction is finished, and collecting a reaction product; and washing the reaction product, carrying out first freeze drying, mixing the product obtained by freeze drying with ampicillin antibiotic, carrying out ultrasonic oscillation, centrifuging, and carrying out second freeze drying to obtain the bovine serum albumin-heme-ampicillin nano-composite. According to the invention, the bovine serum albumin coated heme nano-composite is obtained by a solvent removal method and is embedded with the ampicillin antibiotic, so that good biocompatibility is achieved, and the sterilization efficiency of the ampicillin antibiotic on drug-resistant bacteria is improved.

Embodiments of the invention provide amperometric analyte sensors having optimized elements such as dexamethasone rejection membranes as well as methods for making and using such sensors. The amperometric analyte sensor apparatus comprises: a base layer; a conductive layer disposed on the base layer and comprising a working electrode; an dexamethasone rejection membrane disposed over an electroactive surface of the working electrode, wherein the interference rejection membrane comprises a poly Hema composition and an analyte sensing layer. While embodiments of the innovation can be used in a variety of contexts, typical embodiments of the invention include glucose sensors used in the management of diabetes.

The application discloses a hemoglobin mutant with improved stability and application thereof, and belongs to the technical field of genetic engineering. The application lays a foundation for obtaining an ideal stable human hemoglobin mutant by designing a high-throughput screening method for the structural stability of human hemoglobin and the hematin binding intensification mutant. The application determines hot amino acid sites for the structural stability and thermal stability modification of human hemoglobin through semi-rational design, and further carries out iterative saturation mutation to obtain an ideal mutant rHb 9th The application has the advantages that in terms of structural stability, thermal stability, anti-autoxidation capacity, hematin binding rate, anti-hematin loss rate and human hemoglobin yield, etc. wild‑type The application has the advantages that in terms of structural stability, thermal stability, anti-autoxidation capacity, hematin binding rate, anti-hematin loss rate and human hemoglobin yield, etc.

The application discloses a foliar control agent for reducing cadmium content of rice brown rice and a use method thereof, and relates to the technical field of agriculture. The foliar control agent is composed of the following components: hematin chloride with a concentration of 3-10 micromoles per liter; glutathione with a concentration of 0.1-1 millimoles per liter; NaHS with a concentration of 5-10 micromoles per liter; sodium dodecyl sulfate (SDS) with a concentration of 3-5 millimoles per liter; and water. The compound foliar control agent is sprayed on rice at a tilling stage, a heading stage and a filling stage, respectively, and the spraying amount is 20-40 kg per mu each time. The application utilizes the synergistic effect among hematin chloride, glutathione and NaHS, and adopts a surfactant to expand the solubility of hematin chloride, so that the cadmium reduction effect of rice brown rice is greatly improved, and the cadmium reduction efficiency can reach more than 90%. The foliar control agent has simple components, outstanding synergistic effect of components, simple use and practical use value.

The application provides a low-temperature vacuum-multicavity evaporation linkage heme iron preparation method and device, relates to the technical field of biological medicine and functional food deep processing, and solves the problems of local overheating and fouling caused by traditional wall heat transfer.

A heme iron production process is disclosed. This process includes operations of separating red blood cells from whole blood via centrifuge, collecting the red blood cells, transferring the red blood cells into high speed centrifuges, centrifuging at a speed greater than 6000 rpm, combining effluent red blood cells with reverse osmosis water, increasing temperature and pH of the water, enzymatically digesting the effluent red blood cells into heme iron molecules and waste, and separating the waste from the heme iron molecules.

The invention discloses an antibiotic nano-enzyme capable of reversing drug resistance, and relates to the technical field of biological medicines, and the antibiotic nano-enzyme is formed by assembling an antibiotic and hemin. The antibiotic and hemin are assembled to form the nano-enzyme to reverse the drug resistance of the antibiotic, and the prepared antibiotic nano-enzyme has an excellent antibacterial effect.

The invention discloses a heme preparation method adopting an enzyme method, and relates to the technical field of biological preparation, and the heme preparation method comprises the following steps: S1, taking animal blood and blood powder, carrying out anticoagulation treatment and centrifugal separation to obtain red blood cells, and carrying out cell breaking treatment on the red blood cells to release hemoglobin to obtain a hemoglobin solution; s2, mixing the obtained hemoglobin solution with protease, and carrying out enzymolysis reaction for 1-5 hours under the conditions that the pH value is 3.0-5.0 and the temperature is 20-40 DEG C, so that hemoglobin is subjected to enzymolysis to release heme, and an enzymolysis reaction solution is obtained; s3, precipitating, centrifuging, washing and drying the obtained enzymolysis reaction liquid to obtain a heme product. According to the preparation method of the heme, key links such as raw material pretreatment, enzymolysis reaction and product separation and purification are finely regulated and controlled, so that the heme preparation efficiency and the product quality are remarkably improved, the erythrocyte separation effect is effectively improved, and the release efficiency and the enzymolysis stability of the heme are enhanced.

To provide alternative protein-rich food products that are not derived from animals, but are free of unhealthy meat-related components such as saturated fatty acids and cholesterol, are free of the deleterious environmental effects of animal husbandry, and provide similar texture and flavor characteristics and functional properties as animal meat.SOLUTION: Structured food compositions, such as meat substitute compositions, comprising microbial-derived protein products, such as microbial-derived protein hydrolysates, are provided. Methods of making such products are also provided. Also provided are flavor potentiator compositions comprising heme-containing polypeptides, such as microbial-derived flavohemoglobin, and food compositions, such as meat substitute compositions, comprising the heme-containing flavor potentiator compositions. In one aspect, the flavor potentiator comprises a heme-containing polypeptide from a hydrogen-oxidizing microorganism, wherein (i) the hydrogen-oxidizing microorganism is a Cupriavidus microorganism or (ii) the hydrogen-oxidizing microorganism grows under microaerobic conditions.SELECTED DRAWING: None

This invention belongs to the field of genetic engineering technology, specifically relating to a fusion vitamin D hydroxylase and its application in the preparation of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3. The fusion vitamin D hydroxylase comprises a Vdh-K1 heme domain (as shown in SEQ ID NO.1) and a CYP116B65 redox domain (as shown in SEQ ID NO.3), as well as a linker connecting the two. This fusion vitamin D hydroxylase is a self-sufficient enzyme that can stably catalyze the conversion of vitamin D2 and vitamin D3 without relying on an Fdx and Fdr-assisted electron transport system.

This invention relates to the technical field of pet food, and more particularly to a functional pet nutritional supplement and pet nutritional food and its preparation method, comprising 10%–15% beef liver chunks, 20%–30% chicken breast strips, 35%–45% a mixture of cooked rice flour / corn / rice, 0.3%–1.0% chicken oil, 0.3%–1.0% fish oil, 2%–5% pumpkin puree, 1%–3% chopped carrots, 3%–7% egg yolk powder, 3%–7% a mixture of traditional Chinese medicine extracts, 0.5%–1.5% amino acid and mineral premix, 0.5%–1.5% vitamin and antioxidant premix, 0.0001%–0.001% probiotic mixture, 0.05%–0.3% potassium sorbate, 0.05%–0.3% sodium benzoate, 0.05%–0.2% citric acid, and BHA / BHT. 0.05%–0.2%, with the remainder being chicken broth. Beef liver is rich in heme iron, which, combined with lecithin in egg yolk powder, promotes iron absorption. Traditional Chinese medicine extracts significantly reduce IL-6, IgG, and IgM levels, regulating hyperimmune states. Meanwhile, ingredients such as astragalus enhance normal immunity, achieving a balance of "strengthening the body's resistance and eliminating pathogens."

The present application relates to a kind of intestinal absorption hemin, belong to biological medicine technical field, the intestinal absorption hemin, including hemin and gamma-cyclodextrin;The molar ratio of hemin and cyclodextrin is 1:2;The preparation method of the intestinal absorption hemin, comprising the following steps: (1) hemin is mixed with ammonia water, to obtain hemin solution;Cyclodextrin is mixed with water, to obtain cyclodextrin aqueous solution;(2) the hemin solution is mixed with the cyclodextrin aqueous solution, to obtain mixed solution;(3) the mixed solution is stirred, and dried for 5h, to obtain intestinal absorption hemin.The intestinal absorption hemin prepared in the present application has the advantages of water solubility and stability, high bioavailability after gastrointestinal digestion, and can be used as a raw material for preparing nutritional fortifier for iron supplementation.

The present invention relates generally to a stable liquid formulation of purified hemopexin comprising: (a) a hemopexin content of at least 50 mg / mL; (b) at least 15 mM phosphate buffer; (c) a pH from 5.8 to 8; and (d) at least 50 mM sodium chloride; and uses thereof.

The invention discloses application of an iron-containing substance. The invention finds that the iron-containing substance can be used for preparing the medicine for treating leucopenia and / or thrombocytopenia; the iron-containing substance is selected from myoglobin and a derivative thereof, hemoglobin and a derivative thereof, ferrous sulfate, ferrous fumarate, ferrous gluconate, a polyferose compound, ferrous succinate, iron amino acid chelate, heme, heme iron polypeptide, ferric hydroxide polymaltose, ferrous lactate, ferric pyrophosphate and iron dextran; and one or more of iron hydroxymaltose. The iron-containing substance can effectively increase the number of leukocytes and / or platelets.

The erythromycin fermentation culture medium is optimized by comparing transcriptomics analysis. Specifically, the invention relates to a method for optimizing the fermentation yield of erythromycin by adding six vitamins into a culture medium in a combined manner. More specifically, the six vitamins comprise thiamine pyrophosphate, riboflavin, pyridoxine, folic acid, cobalamin and hemin. The vitamin is added into the fermentation culture medium, so that the growth of erythromycin producing bacteria in the fermentation culture medium is promoted, and the yield of erythromycin is effectively increased.

The present invention discloses a heme iron – glycine chelated iron supplement preparation, belonging to the technical field of nutritional and pharmaceutical preparations. The preparation employs a four-source composite of heme iron, glycine chelated iron, ferrous gluconate, and ferrous lactate, enabling iron supplementation via heme receptor absorption, amino acid chelation pathways, and ionic diffusion. This design overcomes the limitations of single iron sources, including gastrointestinal irritation and low absorption of inorganic iron, dietary inhibition of conventional organic iron, and the high cost or slow onset of novel iron sources. The four iron sources act synergistically to reduce free iron ions and integrate multiple absorption pathways. As a result, gastrointestinal irritation is minimized, and the inhibitory effects of phytic acid, oxalic acid, and tannins are reduced, enabling stable and efficient intestinal absorption. The preparation achieves non-irritating, highly bioavailable, and sustained iron supplementation.

The application discloses a red blood cell drug delivery system of a modified indocyanine green derivative dye, the drug delivery system is loaded with a photosensitizer IR808 on the surface of red blood cells and is wrapped with hematin chloride in the interior of the red blood cells. The application also discloses a use of the near-infrared light response red blood cell drug delivery system of the modified indocyanine green derivative dye in preparation of a drug for treating periodontitis. The drug delivery system can be used for PDT anti-infection treatment in the assistance of NIR laser according to the infection condition of a clinical periodontal disease, and can promote the release of hemin and eliminate potential intracellular bacterial infection. The drug delivery system can be used for intrabag administration in a periodontal pocket according to the clinical requirement of different stages of periodontitis, so that a patient can be free from the trauma of periodontal surgery.

The application discloses a kind of DOC-Chaps synergistic core's clinical and animal tissue rapid transparent reagent and three-dimensional imaging method. Rapid transparent reagent is mainly dissolved in deionized water by sodium deoxycholate (DOC), 3-[(3-cholamide propyl) dimethylamino]-1-propane sulfonic acid (Chaps), urea and 1-methylimidazole; DOC efficiently dissolves high-density lipid, Chaps gently destroys membrane structure, both complement each other, realize lipid rapid, depth clearance and significantly reduce light scattering; urea loosens collagen fiber and protein aggregate, improves the penetration rate of reagent; 1-methylimidazole selectively removes hemoglobin, reduces light absorption. The transparent reagent of the application can make millimeter thick brain slices in 4 (mouse brain) / 6 (human brain) hours The light transmittance of specific wavelength is greater than or equal to 90%, and there is no obvious tissue shrinkage / expansion, the compatibility of fluorescent protein and antibody label is excellent, suitable for three-dimensional imaging of high-lipid, high-hemoglobin samples such as in-vitro human brain, kidney, liver and mouse brain, heart, kidney, liver, intestinal tract, embryo, etc.

Disclosed herein are compounds and methods for preventing and / or treating heme disorders. The compounds are also provided herein for use in the methods. Also disclosed herein are pharmaceutical compositions comprising the compounds for use in methods of preventing or treating heme disorders.

The invention discloses ruddy heme iron red ginseng and donkey-hide gelatin nutritional milk powder and a preparation method thereof, and belongs to the technical field of milk powder, the ruddy heme iron red ginseng and donkey-hide gelatin nutritional milk powder comprises the following components in parts by mass: a wet process part: 420-455 parts of whole milk powder, 306-345 parts of dried skim milk, 185-205 parts of isomaltooligosaccharide (type 50) powder, 35-40 parts of polydextrose, 11.5-14.5 parts of a milk powder nutrition enhancer III, 0.3-0.5 part of compound L-carnitine, and 0.1-0.3 part of heme chloride; according to the ruddy heme iron red ginseng and donkey-hide gelatin nutritional milk powder and the preparation method thereof, the heme chloride is adopted as an iron source, the bioavailability of the red heme iron red ginseng and donkey-hide gelatin nutritional milk powder is remarkably higher than that of ferrous sulfate used in traditional iron-supplementing milk powder, meanwhile, through beta-cyclodextrin embedding treatment, the fishy smell problem of an iron agent is solved, gastrointestinal tract stimulation is avoided, and L-sodium ascorbate is matched, so that the nutrition of the milk powder is improved. And the iron absorption rate can be further improved.