174 results about "Hematinic" patented technology

The present application provides monoclonal antibodies that specifically bind to the hemagglutinin of avian influenza virus subtype H5, as well as monoclonal antibodies capable of blocking at least 50% of the hemagglutinin binding activity of these monoclonal antibodies. Such antibodies are useful, for example, in the detection, diagnosis, prevention, and treatment of avian influenza virus. Also provided herein are hybridoma cell lines, isolated nucleic acid molecules, and short peptides related to the monoclonal antibodies provided herein, and pharmaceutical compositions and kits containing the monoclonal antibodies provided herein.

A method of immunoassay of H5 subtype influenza A virus by which the virus can be accurately assayed even in cases where a certain level of mutation has occurred in the H5 subtype influenza A virus, and a kit therefor, and a novel anti-H5 subtype influenza A virus monoclonal antibody which can be used for the immunoassay are disclosed. The antibody or an antigen-binding fragment thereof of the present invention undergoes antigen-antibody reaction with hemagglutinin of H5 subtype influenza A virus, and the corresponding epitope of the antibody or an antigen-binding fragment thereof is located in a region other than the receptor subdomain (excluding C-terminal region thereof consisting of 11 amino acids), which antibody or an antigen-binding fragment thereof does not have neutralizing activity against the influenza A virus.

The present invention relates to multivalent recombinant raccoon poxviruses, containing more than one exogenous gene inserted into either the thymidine kinase gene, the hemagglutinin gene, or a combination thereof. Disclosed is the use of the multivalent recombinant raccoon poxviruses as vaccines to immunize felines against subsequent challenge by feline pathogens. Also disclosed is a method of making a multivalent recombinant raccoon poxvirus by a recombination process involving the construction of an insertion vector into which the exogenous genes are inserted, and flanking the inserted genes are sequences which can recombine into the raccoon poxvirus thymidine kinase gene, or the hemagglutinin gene, or a combination thereof; introducing both the insertion vector containing the exogenous genes, and raccoon poxvirus into susceptible host cells; and selecting the recombinant raccoon poxvirus from the resultant plaques.

The present invention encompasses methods of producing influenza B viruses in cell culture. The influenza B viruses may have desirable characteristics, such as enhanced replication in eggs and may be used, for example, in vaccines and in methods of treatment to protect against influenza B virus infection.

The present disclosure relates to binding molecules, such as human monoclonal antibodies, that bind to an epitope in the stem region of hemagglutinin of influenza A viruses of phylogenetic group 1 and group 2, as well as influenza B viruses, and have a broad neutralizing activity against such influenza viruses. The disclosure provides nucleic acid molecules encoding the binding molecules, their sequences and compositions comprising the binding molecules. The binding molecules can be used in the diagnosis, prophylaxis and/or treatment of influenza A viruses of phylogenetic groups 1 and 2, as well as influenza B viruses.

The present invention provides one kind of fusion protein in C-terminal of recombinant NMDA receptor NR2B subtype and produced through gene engineering process, polynucleotides encoding the fusion protein, and process of preparing and purifying the fusion protein. The fusion protein consists of the PSD95 linking region segment of NMDA receptor NR2B subtype and the transduction region segment of HIV coded Tat protein, and has one hemagglutinin marker segment for influenza virus inserted between the two above said segments. The fusion protein may be expressed effectively in colibacillus, has simple purifying process and is suitable for production in large scale. The Tat-HA-NR2B9C polypeptide of the present invention can block the interaction between NMDA receptor and PSD95, inhibit NMDA receptor mediated excitable neurotoxicity effect and protect nerves. It may be applied in treating cerebral apoplexy and relevant nerve diseases.

The invention relates to a method using pancreatin-hydrolyzed swine blood corpuscle protein activated by swine fresh pancreas to produce swine blood protein peptide and haemoglobin, belonging to the field of feed additive. The invention takes fresh healthy swine blood corpuscle liquid as a raw material, pancreatin activated by swine fresh pancreas is hydrolyzed and filtered, the filtered supernatant is decolorized, then, the obtained protein liquid is dried to obtain the golden swine blood protein peptide, and the filter cake is dried to obtain the haemoglobin. The technical method has the following innovation points: 1, swine pancreas is taken as the raw material which is fresh and available, and the quality is controllable; 2, pancreatin activation method is simple, enzyme systems are rich, the activated enzyme has high activity, and the hydrolysis effect is good; 3, the swine fresh pancreatin has low cost, which is easy to carry out expanded production; 4, the swine blood corpuscle protein is hydrolyzed by pancreatin which can efficiently separate the haemoglobin thereof and can further prepare haemoglobin by drying the filter cake as the hydrolysate product, thus greatly improving the rate of multipurpose utilization of the swine blood corpuscle protein and avoiding resource waste.

Chimeric Influenza virus-like particles including gag polypeptides are described. Virus-like particles are generated with a gag polypeptide, a neuraminidase polypeptide and optionally a hemagglutinin polypeptide. Preferred methods of generation include expression in insect cells.

The invention discloses a six-flavor hematinic capsule, its quality control method and an application thereof. The six-flavor hematinic capsule is prepared by adding auxiliary materials into raw materials consisting of Chinese angelica, Ligusticum wallichii, Radix Astragali, prepared rehmannia root, lithospermum and white peony root. According to the quality control method of the six-flavor hematinic preparation, whether the six-flavor hematinic capsule contains white peony root, Chinese angelica, Radix Astragali, prepared rehmannia root, lithospermum, Ligusticum wallichii and components is identified by thin layer chromatography; by high-performance liquid chromatography, in vitro dissolution behavior of the six-flavor hematinic capsule is determined, and effective component groups are identified by fingerprint; verbascoside and calycosin glucoside are used as reference substances to simultaneously determine contents in the six-flavor hematinic capsule; and the content of a volatile component ligustilide in the six-flavor hematinic capsule is determined by a gas chromatographic method. The method provided by the invention is simple to operate, is accurate and advanced, has good linear relation, reappearance, precision, stability and recovery rate, can be adopted to effectively control product quality and guarantee curative effect of the product. The invention also provides an application in the preparation of a medicine for treating eye damage and blurred vision.

A glyosylated nitroso hemoglobin pigment for food is prepared from the hemoglobin in the blood of animals or fowls through reacting on sodium nitrite or NO to obtain nitroso hemoglobin, reacting on saccharide to obtain glyosylated nitroso hemoglobin pigment and spray drying. It can be mixed with antioxidizing agent and antiaseptic to obtain a preserving system used for meat. Its advantages are high stability and dispersity, and high effect to decrease the residue of sodium nitrate in preserved meat.

Influenza vaccines with oil-in-water emulsion adjuvants are known. The amount of emulsion adjuvant required for an influenza vaccine can be reduced, thereby allowing more vaccines to be made from a given amount of emulsion, and/or minimizing the amount of emulsion that has to be produced for a given number of vaccine doses. These vaccines can conveniently be made by mixing (i) an oil-in-water emulsion and (ii) an aqueous preparation of an influenza virus antigen. In one aspect, substantially equal volumes of components (i) and (ii) are used; in another aspect, an excess volume of component (ii) is used. When using substantially equal volumes, component (ii) has a hemagglutinin concentration of more than 60 μg influenza virus strain per ml. Components (i) and (ii) can be presented in kit form.

Belonging to the technical field of biomedicine, the invention relates to a sequence and structure of a polypeptide able to specifically bind with influenza virus hemagglutinin protein, and application of the polypeptide in anti-influenza viruses. By expressing purified influenza virus hemagglutinin protein and screening a random peptide library with a phage display technology, a polypeptide specifically bound with influenza virus hemagglutinin and equipped with sequences numbered 1-18 can be obtained. As a hemagglutinin-binding peptide can hinder the combination of hemagglutinin and a host cell receptor, so the influenza virus can be inhibited from infecting the host cell. Thus, the invention also conducts an anti-influenza virus activity study to the hemagglutinin-binding peptide selected from the phase peptide library, and finds that a polypeptide H17, with a sequence of NH2-SHGRITFAYFAN-COOH, can effectively inhibit the influenza virus from infecting the host cell and is of small toxicity. Therefore, the hemagglutinin-binding peptide of the invention and the H17 polypeptide therein with an anti-influenza virus activity are expected to become novel treatment medicaments for treating diseases caused by influenza virus infection and reducing the hazards of diseases caused by influenza viruses.

The invention discloses a comprehensive nutrition powder for reducing fat, lowering blood sugar, lowering blood pressure and lowering blood fat, and a preparation method thereof. The comprehensive nutrition powder comprises nutrients most easily deficient in modern people, auxiliary nutrients for promoting absorption of the nutrients, and effective components for reducing fat, lowering blood sugar, lowering blood pressure and lowering blood fat, and specifically comprises: broccoli powder or carrot powder, black fungus powder, pleurotus eryngii powder, oyster powder, vitamin C, vitamin B2, heme iron, hericium erinaceus cell wall-broken ultrafine powder, composite fermented powder of Chenopodium quinoa and chickpea, olive kernel peptide powder, and composite fermented powder of Sacha Inchikernel and linseed, wherein the pleurotus eryngii powder is dried in the sun. The comprehensive nutrition powder comprehensively considers interaction between various nutrients, eliminates interference of phytic acid on absorption of minerals, and can achieve effects of reducing fat, lowering blood sugar, lowering blood pressure and lowering blood fat from multiple sources and in various aspects.The preparation method can well retain nutrition of dietary ingredients in the comprehensive nutrition powder, and maximize functions of the effective ingredients in reducing fat, lowering blood sugar, lowering blood pressure and lowering blood fat.

Provided are influenza hemagglutinin stem domain polypeptides comprising (a) an influenza hemagglutinin HA1 domain that comprises an HA1 N-terminal stem segment comprising the amino acids from position 1 to position x, preferably from position p to position x, of the HA1 domain, covalently linked by a linking sequence of 0-50 amino acid residues to an HA1 C-terminal stem segment, comprising the amino acids from position y to and including the C-terminal amino acid of the HA1 domain; and (b) an influenza hemagglutinin HA2 domain, wherein the hemagglutinin stem domain polypeptide is resistant to protease cleavage at the junction between HA1 and HA2, and wherein one or more amino acid of the amino acids at positions 337, 340, 352, 353, 402, 406, 409, 413 and/or 416 have been mutated, as compared to the corresponding positions in wild-type influenza HA.

The invention discloses a preparation method of anti-avian influenza virus, newcastle disease virus, chicken bursa virus specific transfer and antimicrobial peptide, which includes the steps of: (1) using avian influenza viral vaccine, newcastle disease virus vaccine, chicken bursa viral vaccine immune experiment pig; (2) closely monitoring vaccine immune antibody level of experiment pig in vivo,when the antibody reaches a peak value, sterilizedly fetching splenic organ or anticoagulated blood of experiment pig for preparing lymphocyte mono-layer; (3) executing subculture for lymphocyte mono-layer; (4) inducing and culturing the lymph subculture cell after which grows into a mono-layer with phaescolosaxin, then the anti-avian influenza virus, newcastle disease virus chicken bursa virus specific transfer and antimicrobial peptide are obtained. The product prepared by the method does not need to be purified further, and can be treated by ultrasonic and freeze thawing simple process intoa mixer for directly being used for bird in vivo, thereby playing roles for resisting avian influenza virus, newcastle disease virus, chicken bursa virus and bacteria, simultaneously improving immunity of bird.

The invention discloses a medicine combination with function of improving nutritional anemia and a preparation method thereof. The medicine combination is prepared by spirulina platensis phycocyanin, vitamin C, vitamin B2 and hemin according to certain weight percent. The medicine combination of the invention has the function of improving nutritional anemia.

The current invention relates to vaccines that use baculovirus vectors to expose a host organism to an immunogen, thereby eliciting an immune response. A pseudo-typed baculovirus is used to display hemagglutinin on the cell membrane in order to increase host immunogenicity.

The invention belongs to the technical field of feeds, and relates to an antibiotic-free composite premix feed for laying hens at a laying period, as well as a preparation method and the application of the feed. The feed consists of the following components in parts by weight: 5-10 parts of corn gluten meal, 8-25 parts of peanut meal, 5-10 parts of rice bran, 15-25 parts of sesame seed meal, 20-50 parts of dietary fibers, 1-10 parts of soybean oil, 5 parts of a 0.5% compound premix, 8-15 parts of stone powder, 8-15 parts of dicalcium phosphate, 2.5-4.5 parts of table salt, 1-3 parts of lysine, 1-3 parts of threonine, 1-3 parts of methionine, 0.1-0.5 parts of Nutri. P namely tannic acid, 1-3 parts of yeast hydrolyate, 0.3-2 parts of yeast cell wall powder, 0.1-0.5 part of selenium yeast, 0.1-0.5 part of zinc methionine, 0.2-0.5 part of hemoprotein, 0.2-0.5 part of a compound enzyme preparation, 0.8 part of choline chloride and 0.2 part of 10000 u/g phytase. Through the adoption of the antibiotic-free composite premix feed, the laying rate, the egg production and the egg weight can be notably increased.

The invention discloses an adenovirus carrier avian influenza recombined vaccine. The vaccine takes the hemagglutinin antigen gene of highly pathogenic avian influenza H5N1 as a main protective antigen gene which is fused with a braided mycobacterium tuberculosis heat shock protein coding gene. After being transferred into an adenovirus carrier to obtain a recombined adenovirus and immunize an animal, the fusion gene can produce a high titer antibody of the hemagglutinin antigen HA inside the animal and keep a high antibody titer inside the animal for a long period.

A method for separating and purifying the active hematinic species (AHS) present in iron-saccharidic compositions, including AHS such as sodium ferric gluconate complex, ferric hydroxide-sucrose complex and ferric saccharate complex and others of similar form and function. The method separates the AHS from one or more excipients and, preferably, lyophilizes the separated AHS. Separation of the AHS permits its analytical quantification, further concentration, purification and/or lyophilization as well as preparation of new and useful products and pharmaceutical compositions.