66 results about "Fluorescent polarization" patented technology

The invention discloses a method of determining protease activity, in real time, using fluorescence polarization technology. In particular, the invention provides vectors and a method for their use, which expresses uncharacterized proteins conjugated to a fluorescence tag, which binds specifically to a fluorescent ligand. Cleavage of the recombinant protein results in a fragment of the expressed peptide and results in a change in fluorescence polarization of the fluorophore. The rate of change in fluorescence polarization can be measured in real time and is equivalent to the rate of protease cleavage.

The invention relates to a fluorescent polarization based homogeneous phase detection method of a single nucleotide polymorphism of a codon118 of an ERCC1 (excision repair cross-complementing 1) gene. The homogeneous phase detection method solves the problems of high cost, complicated operating steps, proneness to causing contamination and influence on detection result accuracy in the prior art, and is simple in steps, easy to operate, not prone to causing contamination as operation can be completed once in a closed tube, and low in cost as any special reagent and fluorescence quenching or micro-groove binding agent are not needed. Application of a specific probe with single end marked to detection of the single nucleotide polymorphism of the codon118 of the ERCC1 gene results in changes of fluorescent polarization values and enables detection results to be more objective and accurate. Result analysis is simple as only numeric comparison is needed in result judgment, and the homogeneous phase detection method can easily realize standardization and automation, is wide in application range and can be used for detecting clinical blood or tissue specimens.

The invention relates to a homogeneous phase detection method for a methylation state of an epidermal growth factor receptor (EGFR) gene promoter based on fluorescence polarization. The method solves the problems that the cost is high, the operation steps are fussy, pollution is easily produced and the accuracy of the detection result is affected in the prior art; the method is simple and easy to operate; and the method is finished by one step in a closed tube, and does not produce pollution. The method is low in cost, and does not require any special reagent and fluorescence quenching or micro groove bonding agent. Methylation of a target region is detected by using change of a fluorescence polarization value due to a single end-marked methylated specific probe, so that the detection result is objective and accurate. The method is simple in result analysis; and only digital comparison is required during result judgment, so the method easily realizes standardization and automation, has a wide application range and can be used for detecting clinical blood or tissue specimens.