66 results about "Fluorescent polarization" patented technology

This invention relates to a laser especially high power solid red, yellow, blue laser device including a pump laser, two cavity mirrors, a laser crystal, nonlinear optical crystal, and one or more than two tuned prisms, in which the pump laser is mounted in front of a cavity mirror, the laser crystal tuned prism and nonlinear optical crystal are orderly mounted on the optical path between two cavity mirrors in which the tuned prism is fixed on an angle tunable horizontal rotating stand. Other optical elements are mounted on a angle tunable optical platform applying a prism and two cavity mirrors to constitute a single path or double paths frequency multiplication light path overcoming the shortcoming of difficult to coat film or to suppress 1064 nm fluorescent polarization.

The invention provides a fluorescence polarization immunoassay method for detection of erythromycin. The method includes: subjecting an erythromycin standard substance solution with a known concentration to mixed incubation respectively with a fluorescent marker solution and an erythromycin monoclonal antibody solution to carry out competing reaction, thus obtaining a fluorescence polarization value of the system; taking the measured fluorescence polarization value as the ordinate, and adopting the concentration of a series of sulfamethazine standard substances with known concentration as the abscissa to draw a standard curve; using a to-be-measured sample to replace the erythromycin standard substance in step 1), according to the operation in step 1), determining the fluorescence polarization value of the system after incubation, and according to the standard curve, calculating the erythromycin concentration of the to-be-measured sample. The invention provides the homogeneous and rapid erythromycin fluorescence polarization immunodetection method, which only needs sample adding, has no need of separation and washing operation, and can obtain the detection result within 10min.

A method of determining a membrane potential is disclosed. The method comprises (a) determining a difference in fluorescence polarization of a charged fluorescent probe being distributed across the membrane; and (b) determining a potential of the membrane, wherein the potential is proportional to the difference in the fluorescent polarization.

The inventive subject matter relates to a competitive method for estimating the concentration in a sample of a Bacillus anthracis protein or antibody thereof selected from the group consisting of protective antigen (PA), lethal factor (LF) and edema factor (EF). The method may employ the use of Fluorescence Polarization, FLT or FRET. The competitive methods are capable of detecting a target protein within 5 minutes within the range of 0.1 to 10.0 nM. The methods provide for the rapid detection and quantitation of bacteria, bacterial antigen or antibody in culture media or broth of growing cultures of bacteria, including B. anthracis by fluorescent methods.

The invention discloses a method of determining protease activity, in real time, using fluorescence polarization technology. In particular, the invention provides vectors and a method for their use, which expresses uncharacterized proteins conjugated to a fluorescence tag, which binds specifically to a fluorescent ligand. Cleavage of the recombinant protein results in a fragment of the expressed peptide and results in a change in fluorescence polarization of the fluorophore. The rate of change in fluorescence polarization can be measured in real time and is equivalent to the rate of protease cleavage.

Methods, systems, kits for carrying out a wide variety of different assays that comprise providing a first reagent mixture which comprises a first reagent having a fluorescent label. A second reagent is introduced into the first reagent mixture to produce a second reagent mixture, where the second reagent reacts with the first reagent to produce a fluorescently labeled product having a substantially different charge than the first reagent. A polyion is introduced into at least one of the first and second reagent mixtures, and the fluorescent polarization in the second reagent mixture relative to the first reagent mixture is determined, this fluorescent polarization being indicative of the rate or extent of the reaction.

Disclosed relates to a method and an apparatus for measuring fluorescence polarization FP in a lab-on-a-chip and, more concretely, to a method and an apparatus that measure quantitatively interactions between biomolecules and fluorescently labeled biomaterials and enzyme activities using the measurement of fluorescence polarization FP. The method and the apparatus of the invention provide rapid assays with minute amounts of samples using an automated device compared to conventional methods. Accordingly, the method and the apparatus of the invention can be usefully applied to the measurement of interactions between biomolecules and to the protease assays using a protein substrate.

The invention relates to a fluorescent polarization based homogeneous phase detection method of a single nucleotide polymorphism of a codon118 of an ERCC1 (excision repair cross-complementing 1) gene. The homogeneous phase detection method solves the problems of high cost, complicated operating steps, proneness to causing contamination and influence on detection result accuracy in the prior art, and is simple in steps, easy to operate, not prone to causing contamination as operation can be completed once in a closed tube, and low in cost as any special reagent and fluorescence quenching or micro-groove binding agent are not needed. Application of a specific probe with single end marked to detection of the single nucleotide polymorphism of the codon118 of the ERCC1 gene results in changes of fluorescent polarization values and enables detection results to be more objective and accurate. Result analysis is simple as only numeric comparison is needed in result judgment, and the homogeneous phase detection method can easily realize standardization and automation, is wide in application range and can be used for detecting clinical blood or tissue specimens.

The invention relates to a homogeneous phase detection method for a methylation state of an epidermal growth factor receptor (EGFR) gene promoter based on fluorescence polarization. The method solves the problems that the cost is high, the operation steps are fussy, pollution is easily produced and the accuracy of the detection result is affected in the prior art; the method is simple and easy to operate; and the method is finished by one step in a closed tube, and does not produce pollution. The method is low in cost, and does not require any special reagent and fluorescence quenching or micro groove bonding agent. Methylation of a target region is detected by using change of a fluorescence polarization value due to a single end-marked methylated specific probe, so that the detection result is objective and accurate. The method is simple in result analysis; and only digital comparison is required during result judgment, so the method easily realizes standardization and automation, has a wide application range and can be used for detecting clinical blood or tissue specimens.

Methods, systems, kits for carrying out a wide variety of different assays that comprise providing a first reagent mixture which comprises a first reagent having a fluorescent label. A second reagent is introduced into the first reagent mixture to produce a second reagent mixture, where the second reagent reacts with the first reagent to produce a fluorescently labeled product having a substantially different charge than the first reagent. A polyion is introduced into at least one of the first and second reagent mixtures, and the fluorescent polarization in the second reagent mixture relative to the first reagent mixture is determined, this fluorescent polarization being indicative of the rate or extent of the reaction.

Methods, systems, kits for carrying out a wide variety of different assays that comprise providing a first reagent mixture which comprises a first reagent having a fluorescent label. A second reagent is introduced into the first reagent mixture to produce a second reagent mixture, where the second reagent reacts with the first reagent to produce a fluorescently labeled product having a substantially different charge than the first reagent. A polyion is introduced into at least one of the first and second reagent mixtures, and the fluorescent polarization in the second reagent mixture relative to the first reagent mixture is determined, this fluorescent polarization being indicative of the rate or extent of the reaction.