47 results about "ERCC1" patented technology

The invention provides a kit for detecting the expression level of ERCC1 (excision repair cross complementation 1). By adopting a fluorescent quantitative PCR (polymerase chain reaction) platform, and taking RNA (ribonucleic acid) reserved by culture cells as external calibration RNA and GapDH (reduced glyceraldehydes-phosphate dehydrogenase) as data of internal contrast gene normalization processing experiments, relative quantitative detection is carried out on the level of mRNA (messenger ribose nucleic acid) of ERCC1 of a sample tissue through a TaqMan probe method. The kit can be used for detecting the expression quantity of the ERCC1 at a level of mRNA and can reflect the gene expression level more truly; the detection data of the fluorescent quantitative PCR method is output by a machine, thus avoiding subjective identification of people and being more objective; the cultured cells are taken as the external RNA calibrator, and the whole process and the fluorescent quantitative PCR experiment are synchronous, so that the errors caused by the experiment process is reduced, the determination of the clinical cut-off value is realized, furthermore, the fluorescent quantitative PCR method for detecting the level of mRNA of ERCC1 is realized to be used in clinic.