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Specific egg-yolk antibody for preventing and treating feline distemper and preparation method of specific egg-yolk antibody

A yolk antibody, specific technology, applied in chemical instruments and methods, specific peptides, antiviral immunoglobulin, etc., can solve the problem of insignificant curative effect

Active Publication Date: 2019-01-01
GUANGXI VETERINARY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since there is no specific drug against viral diseases, for the diseased animals, clinically, symptomatic treatment or antibiotics are mainly used to prevent complications, and the curative effect is not significant

Method used

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  • Specific egg-yolk antibody for preventing and treating feline distemper and preparation method of specific egg-yolk antibody
  • Specific egg-yolk antibody for preventing and treating feline distemper and preparation method of specific egg-yolk antibody

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Embodiment Construction

[0023] One, the preparation of FPV inactivated vaccine

[0024] The FPV-GX01 strain was synchronously inoculated into FK81 cat kidney cells at a volume of 1 / 10, at 37°C CO 2 After standing in the incubator for 24 hours, discard the growth medium and replace it with a maintenance medium to continue culturing. Observe the cell changes every day. When 75% of the cells become diseased, collect the cells, freeze and thaw 3 times, and then centrifuge at 12,000 rpm for 15 minutes at 4°C to remove Cell debris, collect supernatant, determine its TCID 50 / 0.1mL value ≥ 5.0, hemagglutination titer ≥ 1:2 10 Finally, add thymosin to a final concentration of 0.3mg / mL, add formaldehyde to a final concentration of 0.1%, inactivate at 37°C for 24 hours, shake once every 4 hours, then add 4% Tween-80 as the water phase; Span80 and No. 10 white oil was mixed as the oil phase at a volume ratio (V / V) of 3:47, and the water phase and the oil phase were mixed at a volume ratio of 1:2 for water-in-...

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PUM

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Abstract

The invention discloses a specific egg-yolk antibody for preventing and treating feline distemper. The specific egg-yolk antibody is prepared by taking FPV inactivated vaccine and FPV subunit vaccineas immunogens and using immune laying hens. Accordingly, a corresponding preparation method is established and comprises the following steps: creatively inoculating laying hens with two different FPVimmunogens, taking the FPV inactivated vaccine as a basic immunogen and the FPV subunit vaccine as a strengthened immunogen; optimizing an immunization method and inoculation dose to produce high antibody titer; immunizing the laying hens, then harvesting eggs from hype-immunized chickens, separating egg yolk, and extracting and purifying a coarse product of the yolk antibody, thus obtaining the specific egg-yolk antibody for preventing and treating the feline distemper. The test shows that the yolk antibody disclosed by the invention has the advantages of good safety, high specificity, high antibody titer, low cost and high yield; the specific egg-yolk antibody can be used for treatment and emergency prevention of the feline distemper and enhancing the resistance of sick cats and has a good popularization and application prospect.

Description

technical field [0001] The invention belongs to the technical field of feline distemper treatment, and in particular relates to a specific yolk antibody for preventing and treating feline distemper and a preparation method thereof. Background technique [0002] Feline distemper, also known as feline panleukopenia, feline infectious enteritis, etc., is an acute, highly contagious and fatal infectious disease of cats caused by feline parvovirus (FPV). The main features are diarrhea, severe leukopenia, and it is the most common infectious disease in domestic cats. FPV has only one serotype, and it can infect various animals such as cats, raccoons and mustelids under natural conditions, among which small cats and minks are most susceptible. FPV can be infected through direct or indirect contact. The infected animals excrete toxins through feces, urine, vomit, saliva and other excretions and secretions, polluting the environment, utensils, feed, etc., causing direct and indirect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C07K16/02
CPCC07K16/081C07K2317/11
Inventor 吴健敏刘金凤覃绍敏张振江白安斌唐海波陈凤莲马玲林俊秦树英杨磊
Owner GUANGXI VETERINARY RES INST
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