Method for preparing IgY for SARS
An atypical pneumonia and egg yolk antibody technology, applied in the field of biological drug production, can solve the problems of low IgG yield, high cost, limitations, etc., and achieve the effects of strong resistance to external factors, long duration, and small antigenic amount
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[0015] The preparation method of anti-atypical pneumonia egg yolk antibody, the method comprises: making vaccinogen, antibody preparation, egg yolk homogenate, dilution, low temperature overnight, filtration and purification, and powder making.
[0016] The present invention is based on the attached figure 1 The specific process route given is completed, and the specific process is explained in detail as follows:
[0017] 1. Preparation of immunogen. The SARS virus culture was selected to be inactivated by β-propiolactone, and polyethylene glycol-6000 was concentrated 8-15 times to make the water phase. MAUTAMIDEISA-70 is an oil phase, emulsified into a water-in-oil immunogen at a high speed according to the ratio of 15-20:50-80.
[0018] 2. Preparation of anti-SARS poultry yolk antibody. Select 100-140-day-old SPF poultry, for example, 120-day-old SPF poultry, immunize 1.5-3.5ml for the first time, and inject 2-4 sites in the chest muscle. Repeat the booster immunization ...
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