Method for preparing IgY for SARS

An atypical pneumonia and egg yolk antibody technology, applied in the field of biological drug production, can solve the problems of low IgG yield, high cost, limitations, etc., and achieve the effects of strong resistance to external factors, long duration, and small antigenic amount

Inactive Publication Date: 2004-11-17
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

However, the IgG provided by these sources is low in yield and high in cost, and is usually limited to laboratory immunological diagnosis and basic research

Method used

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  • Method for preparing IgY for SARS

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Embodiment 1

[0015] The preparation method of anti-atypical pneumonia egg yolk antibody, the method comprises: making vaccinogen, antibody preparation, egg yolk homogenate, dilution, low temperature overnight, filtration and purification, and powder making.

[0016] The present invention is based on the attached figure 1 The specific process route given is completed, and the specific process is explained in detail as follows:

[0017] 1. Preparation of immunogen. The SARS virus culture was selected to be inactivated by β-propiolactone, and polyethylene glycol-6000 was concentrated 8-15 times to make the water phase. MAUTAMIDEISA-70 is an oil phase, emulsified into a water-in-oil immunogen at a high speed according to the ratio of 15-20:50-80.

[0018] 2. Preparation of anti-SARS poultry yolk antibody. Select 100-140-day-old SPF poultry, for example, 120-day-old SPF poultry, immunize 1.5-3.5ml for the first time, and inject 2-4 sites in the chest muscle. Repeat the booster immunization ...

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Abstract

The invention discloses a method for preparing IgY for SARS, which belongs to a process for manufacturing anti-SARS biological pharmaceuticals. The process comprises the steps of making vaccinogen, preparing antibody, homogenating vitelline, diluting, low-temperature stewing, filtering, purifying, and pulverizing. The said antibody preparation process is disclosed in the invention. The process according to the invention can be applied in preparing anti-SARS medicament.

Description

Technical field: [0001] The invention relates to a production method of anti-atypical pneumonia yolk antibody, which is a production method of biological medicine. Background technique: [0002] Since the first case of atypical pneumonia (Severe Acute Respiratory Syndrome, SARS) was discovered in Foshan, Guangdong, China on November 16, 2002, SARS first spread in Guangdong Province and spread rapidly to all parts of the world, according to the World Health Organization (WHO ) according to the latest statistics, as of April 26, 28 countries and regions in the world have become SARS-incidence areas, with a total of 4,836 SARS cases and 293 deaths worldwide. The number of patients in mainland China has reached 2,753, with 122 deaths. my country's Guangdong Province became the initial outbreak and large-scale epidemic area of ​​SARS. From the moment SARS occurred, people have carried out a lot of investigations on the real culprit of the disease - the pathogen, and isolated th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61P11/00A61P31/14C07K16/02
Inventor 孔宪刚王治国付朝阳王笑梅高洪雷周琦
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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