75 results about "Coating antigen" patented technology

The invention provides an ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting an anti-MDA5 antibody and a preparation method of the ELISA kit. According to the kit, full-length human MDA5 protein (1025 amino acids) expressed by a cell-free protein expression system under the low-temperature condition of 22 DEG C is adopted as a coating antigen, and a disulfide bond enhancer E6820 is added into the system to promote correct folding of the protein. The kit comprises a microwell plate coated with a full-length MDA5 antigen, an enzyme-labeled anti-human IgG secondary antibody, a standard substance with the concentration range of 1-243 ng / mL, a chromogenic substrate and an optimized confining liquid system. The invention solves the problem of insufficient sensitivity caused by the use of fragment antigens in the prior art, and can be used for early diagnosis and illness monitoring of dermatomyositis-related interstitial lung diseases.

The invention is applicable to the technical field of biology, and provides an L7 / L12-PADRE sequence-multi-linked B cell epitope recombinant protein and a kit for detecting an anti-Brucella antibody. The recombinant protein is a multi-epitope tandem recombinant protein composed of Brucella ribosome L7 / L12 protein, a PADRE polypeptide sequence and multiple B cell epitopes, the amino acid sequence of the recombinant protein is as shown in SEQ ID No.1, and the recombinant protein is good in antigenicity. The anti-brucella antibody indirect ELISA (iELISA) detection method and kit established by taking the recombinant protein as the coating antigen have the characteristics of high sensitivity, strong specificity and good repeatability, are suitable for detecting the condition of generating the anti-brucella antibody by an organism, can clarify the immune background of brucella infection or brucellosis vaccine, and can be used for detecting the brucella infection or brucellosis vaccine. Basic data is provided for prevention and control of the Brucella disease of humans and animals, and meanwhile, the kit is conveniently applied to large-scale sample detection and epidemiological monitoring.

The invention discloses a recombinant CapA protein of cat astrovirus. The recombinant CapA protein has an amino acid sequence as shown in SEQ ID NO.1 in a sequence table or is coded by a base sequence as shown in SEQ ID NO.2 in the sequence table. Therefore, a cat astrovirus indirect ELISA antibody detection kit is developed and comprises a coated ELISA plate, and the coated ELISA plate takes the recombinant CapA protein as a coating antigen. Meanwhile, a corresponding indirect ELISA antibody detection method is established. Research results show that the critical value of the indirect ELISA antibody detection method and kit is 0.523; the sensitivity is 1: 32000; the in-batch variation coefficient of detected samples is 2.679%-7.910%, the inter-batch variation coefficient is 3.627%-8.982%, both the in-batch variation coefficient and the inter-batch variation coefficient are smaller than 10%, and the clinical application effect is good. Therefore, the kit has the characteristics of high sensitivity, strong specificity, good repeatability and the like, can quickly and effectively detect the FAstV antibody, and provides technical support for accurate diagnosis, timely prevention and control and vaccine effect evaluation of FAstV.

The application relates to a sensor for synchronously detecting enniatin and roquefortine based on a double-potential electrochemiluminescence immunoassay technology as well as a preparation method and a detection method thereof. The sensor comprises a base electrode, a sensing interface composed of a nanocomposite is modified on the surface of the base electrode, mixed coated antigens including ENNs-BSA and AOH-BSA fixed on the sensing interface, mixed probes including a cathode probe and an anode probe, the cathode probe is Zr-Por-MOF-ENNs Ab which is obtained by marking ENNs antibodies with a porphyrin zirconium metal organic framework, the anode probe is Ce-In-MOF@ABEI-AOH Ab which is obtained by marking AOH antibodies with ABEI loaded on a cerium-doped indium metal organic framework, and a detection solution containing a single co-reactant potassium persulfate. The application can realize one-time sample adding, double-potential scanning and synchronous quantification for two kinds of mycotoxins of enniatin and roquefortine.

This invention discloses a quantitative test strip for HBV PreS1 antibody based on the AIE molecule, its preparation method, and its applications, relating to the fields of molecular biology, immunology, and biodetection technology. The test strip includes a sample pad, a labeling pad, a nitrocellulose membrane (NC membrane), an absorbent pad, and a base plate. The NC membrane detection line is coated with HBV PreS1 antigen to capture PreS1 antibodies in the sample. The labeling pad contains an immobilized Anti-Human IgG antibody conjugated to the AIE molecule, which binds to the PreS1 antibodies in the sample to form an immune complex. The test strip of this invention enables rapid detection of PreS1 antibodies and achieves enhanced fluorescence in the detection results. Combined with a fluorescence reader or fluorescence card reader, the detection results can be digitally and quantitatively analyzed. It is simple to operate, rapid in detection, and significantly improves detection sensitivity and enables quantitative analysis.

The application relates to a detection method of anti-preS1 antibody, an anti-preS1 antibody detection kit and application, the application carries out multi-sequence comparison and conservation analysis on preS1 sequences of different genotypes (A, B, C, D and CD mixed type) of HBV, takes type A (AB697487.1), type C (FJ032351.1) and type D (LC519808.1) as reference sequences, based on the sequence consistency standard of 85% of full length and 90% of the key region of functional antibody binding, a highly conservative preS1 full-length antigen sequence is systematically screened out in natural variation, the sequence can cover antigen variation of A-D type HBV, and the sequence is taken as a coating antigen to establish an indirect ELISA detection system, a good linear relationship is presented in the range of 4.88-625 ng / mL, and the Cut-off value is 33.6 ng / mL; clinical verification shows that the method can effectively distinguish chronic hepatitis B patients from healthy control groups, compared with detection methods based on partial fragments or overlapping short fragments, the false negative rate can be significantly reduced, and the method is suitable for HBV infection immune state evaluation, anti-virus curative effect monitoring and prognosis analysis.

This invention discloses an indirect ELISA detection kit and method for bovine pasteurellosis, relating to the field of biotechnology. The indirect ELISA kit includes an enzyme-labeled plate coated with an antigen; the amino acid sequence of the antigen is shown in SEQ ID NO.2. The indirect ELISA detection kit constructed in this invention exhibits high specificity and sensitivity; the intra-assay coefficient of variation is between 1.79% and 7.54%, and the inter-assay coefficient of variation is between 2.23% and 6.91%, both below 10%, demonstrating good inter-assay and intra-assay repeatability. In testing 30 clinical samples, the overall concordance rate with the standard "Diagnostic Techniques for Bovine Hemorrhagic Sepsis (GB / T27530-2025)" was 96.67%. The indirect ELISA detection kit demonstrates good overall performance and can be applied to the detection of bovine pasteurellosis in clinical serum samples.

The invention provides a linear epitope of an African swine fever virus CP312R protein. The amino acid sequence of the linear epitope is SEQ ID NO: 1 or SEQ ID NO: 2. The antigen epitope polypeptide provided by the invention has good antigen specificity and immunoreactivity. The polypeptide can be efficiently combined with an African swine fever virus specific antibody, background interference is low, cross reaction is small, and detection sensitivity and accuracy are remarkably improved. By using the polypeptide as a coating antigen in immunodetection methods such as ELISA or colloidal gold immunochromatography, rapid and accurate detection of ASFV infection can be realized, and the detection result is stable and reliable. Compared with a traditional whole virus antigen or a crude extract antigen, the epitope polypeptide has the advantages of being simple and convenient to prepare, small in batch difference, high in safety and the like, and is suitable for ASFV serological monitoring and diagnosis application.

The invention discloses a method and a kit for simultaneously detecting avian leukosis virus, mycoplasma synoviae and salmonella pullorum antibodies, and belongs to the technical field of animal epidemic disease detection. According to the method, an avian leukosis virus p27 protein, a chicken mycoplasma synoviae p50 protein and a truncated GroEL-delta8-1 protein of a dominant antigen GroEL of salmonella pullorum are taken as coating antigens, synchronous detection of the three antibodies is realized by optimizing ELSA reaction conditions, and a kit capable of simultaneously detecting the three antibodies is developed at the same time. The kit has the characteristics of strong specificity, high sensitivity and simplicity and convenience in operation, is suitable for rapid screening and purification of poultry epidemic diseases, and reduces the detection cost.

The invention discloses a polypeptide-based avian reticuloendotheliosis virus indirect ELISA antibody detection method, and belongs to the technical field of biology. According to the kit, potential epitopes of REV gp90 protein are systematically screened to obtain three sections of specific polypeptide antigens for detecting the avian reticuloendotheliosis virus antibody, and a mixed product of the three sections of specific polypeptide antigens is used as a coating antigen to develop the indirect ELISA antibody detection kit for the avian reticuloendotheliosis virus. On the basis of the indirect ELISA antibody detection kit, the avian reticuloendotheliosis virus antibody detection method established by optimizing the coating concentration, the sealing condition, the serum incubation time and the like has the advantages of strong specificity, high sensitivity, good repeatability and convenience in operation; the kit can effectively and rapidly diagnose epidemic diseases caused by the avian reticuloendotheliosis virus, and is beneficial to popularization and application.

This invention discloses a kit for detecting porcine reproductive and respiratory syndrome virus (PRRSV) antibodies and its applications, belonging to the field of animal disease detection technology. The kit uses a mutant nucleocapsid protein (N protein) of the PRRSV-2 class NADC30 strain CHsx1401 as the coating antigen; the N protein mutant has mutations at amino acid positions 23, 75, and / or 90 compared to the wild-type N protein. High purity is achieved through optimized prokaryotic expression systems and purification processes. Under optimal detection conditions, the kit exhibits strong specificity, high sensitivity, and good reproducibility, with significantly superior detection performance compared to existing commercial kits. It can be used for PRRSV-2 antibody detection, epidemiological surveys, and disease control.

This invention constructs pET-32a-CP1 and pET-32a-CP2 expression vectors based on the CP1 and CP2 protein genes of Giardia duodenalis, transforms them into Rosetta (DE3) competent cells, and induces expression using IPTG. After SDS-PAGE analysis of the recombinant protein expression, Ni-NTA affinity chromatography is used to purify the proteins. Two indirect ELISA methods are then established using these as coating antigens. The indirect ELISA methods based on the recombinant proteins CP1 and CP2 of this invention exhibit good specificity, sensitivity, and repeatability, with concordance rates of 98% and 94% with microscopic detection results, respectively.

The invention discloses an OVA-D fluorescence immunochromatography detection test strip in a surimi product and application thereof, the test strip comprises a substrate, a sample adding pad, a combination pad, a reaction film and a water absorption pad, the sample adding pad, the combination pad, the reaction film and the water absorption pad are arranged on the substrate, the reaction film is arranged in the middle of the substrate, one end of the sample adding pad is in contact with the other end of the combination pad, one end of the combination pad is in contact with the other end of the reaction film, and the other end of the reaction film is in contact with the water absorption pad. One end of the reaction film contacts with the other end of the water absorption pad; the combination pad contains a rabbit anti-OVA-D polyclonal antibody marked by fluorescent quantum dots and goat IgG marked by fluorescent quantum dots; the reaction film is provided with a detection area, the detection area is sequentially provided with a detection line and a control line from the other end to one end of the reaction film, the detection line is coated with an OVA-D antigen, and the control line is coated with a rabbit anti-goat IgG antibody. The fluorescence signal is stable, the fluorescence intensity of the T line and the C line is not influenced by a complex matrix in a detected sample, the detection sensitivity is higher, and the reliability of quantitative detection of the duck egg ovalbumin is also ensured.

The invention provides an indirect ELISA (Enzyme-Linked Immunosorbent Assay) antibody detection method and an indirect ELISA antibody detection kit based on pyruvate Kinase (PK), and relates to the technical field of parasite detection. The kit comprises a coating antigen Italiella mannici PK protein, a primary antibody, a labeled secondary antibody and an initiator solution, wherein the primary antibody is a mouse or cat anti-Italiella mannici PK protein antibody to be detected; the labeled secondary antibody can be combined with the primary antibody combined with the PK protein of the andrometrium mansoni; and the labeled second antibody is provided with a detection marker. The method comprises the following steps: cloning a PK protein gene into a prokaryotic expression vector PQE80L (+), constructing a recombinant plasmid PQE80L-PK, inducing PK recombinant protein expression by utilizing IPTG (isopropyl-beta-d-thiogalactoside), purifying the recombinant protein to coat an elisa plate, and establishing an indirect ELISA (enzyme-linked immuno sorbent assay) detection method of the Mangnocorynth PK protein antibody by optimizing reaction conditions. The detection method has the advantages of high sensitivity and strong specificity, and provides support for the development of the detection technology of the sitonia mansoni and the prevention and control of the schizocyst.

The invention discloses a lateral flow biosensor for joint detection of SAA and CRP based on an AIE material and application, the lateral flow biosensor comprises a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the combination pad is coated with fluorescent nano-microsphere labeled antibodies AIE490NP-SAA, AIE490NP-RIgG and AIE610NP-CRP, and the nitrocellulose membrane is coated with fluorescent nano-microsphere labeled antibodies AIE490NP-RIgG and AIE610NP-CRP. A detection line T line and a quality control line C line are sprayed on the nitrocellulose membrane, the T line is formed by an SAA coating antigen and a CRP coating antigen which are coated on the nitrocellulose membrane, and the C line is formed by a goat anti-rabbit IgG antibody coated on the nitrocellulose membrane. The prepared lateral flow biosensor can simultaneously detect CRP and SAA, has high sensitivity and good repeatability, and has wide application prospects in differential diagnosis of early infectious diseases.

The invention discloses a chemiluminiscence detection reagent for detecting an HCV antibody and a use method of the chemiluminiscence detection reagent, and relates to the technical field of immunodetection. The detection reagent is combined with a sandwich method and an indirect method for detection. When in use, a sample is incubated with the first reagent and the sample treating fluid, so that the HCV antibody to be detected in the sample reacts with the HCV coating antigen in the first reagent to form an antigen-antibody compound; adding a second reagent, and reacting the formed antigen-antibody compound with the HCV labeled antigen labeled by acridinium ester and the anti-IgG antibody labeled by acridinium ester in the second reagent to form an antigen-antibody-antigen compound and an antigen-antibody-secondary antibody compound; and finally, adding excitation liquid to enable acridinium ester to emit light, and detecting the HCV antibody in the sample by detecting a light-emitting signal value. The technical scheme of the invention greatly improves the sensitivity and specificity of HCV antibody detection, and can be used for solving the problem of easy missing detection in HCV antibody detection.

The invention relates to the technical field of virus detection, in particular to CCEV-99 recombinant protein, application of the CCEV-99 recombinant protein and an indirect ELISA detection method of a camel contagious pustular dermatitis virus antibody. In a kit used in the method, an ELISA plate is coated with CCEV-99 recombinant protein. The CCEV-99 recombinant protein can be used as a coating antigen of the camel contagious pustular dermatitis virus indirect ELISA detection kit, and rapid detection of a camel contagious pustular dermatitis virus specific antibody is realized; the detection method has the advantages of being good in specificity, sensitivity and repeatability, easy and convenient to operate, low in cost, capable of achieving large-scale detection and the like. The method is efficient, accurate and practical, and provides powerful technical support for follow-up research of camel contagious pustular disease.

The invention discloses a monospecific monoclonal antibody for resisting zearalenone-14 glucoside and application of the monospecific monoclonal antibody. An immunogen and a coating antigen are used; the monoclonal antibody capable of monospecifically recognizing the zearalenone-14 glucoside and the rapid detection kit for detecting the zearalenone-14 glucoside are obtained through the technologies of mouse immunization, serum detection, cell fusion, monoclonal screening, ascites induced antibody and the like. The method is suitable for single-specificity trace residue detection of the zearalenone-14 glucoside in samples such as corn, rice, millet, wheat and oat, has very high recognition sensitivity to the zearalenone-14 glucoside, and realizes rapid, large-batch and low-cost detection of the zearalenone-14 glucoside.

The application discloses a kind of for HBV PreS1 antibody qualitative detection test strip and its preparation method and application, it is related to virology, immunology, biological detection technology and immunochromatography analysis technical field.The test strip is based on the specific recognition principle of antigen and antibody, the specific binding of PreS1 antigen and PreS1 antibody is realized, the rapid detection of PreS1 antibody in serum or plasma sample, it is sequentially assembled by sample pad, gold mark pad, nitrocellulose membrane (NC membrane) and water absorption pad, wherein, gold mark pad is fixed with colloidal gold labeled anti-human IgG antibody as gold mark probe, NC membrane detection line (T line) is coated HBV PreS1 antigen, for capturing PreS1 antibody in sample, NC membrane quality control line (C line) is coated HB2 antibody, for judging whether the test strip detection process is effective.The test strip in the application does not need complex instrument equipment, detection process is simple, fast and fast, and has good sensitivity and specificity, can be used for hepatitis B vaccine immune effect evaluation and large-scale screening.

The invention belongs to the technical field of antigen-antibody detection, and relates to a monoclonal antibody of a non-biphenyl tetrazolsartan drug, a nucleic acid molecule, a detection kit and application thereof. The monoclonal antibody of the non-biphenyl tetrazolsartan medicine comprises a heavy chain variable region (VH) and a light chain variable region (VL), the heavy chain variable region comprises a VH-CDR1, a VH-CDR2 and a VH-CDR3, the light chain variable region comprises a VL-CDR1, a VL-CDR2 and a VL-CDR3, the sequence of the VH-CDR1 is as shown in SEQ ID NO.1, and the sequence of the VH-CDR2 is as shown in SEQ ID NO.2. The monoclonal antibody of the non-biphenyl tetrazolsartan medicine has the advantages that the monoclonal antibody of the non-biphenyl tetrazolsartan medicine can be used for preparing the non-biphenyl tetrazolsartan medicine; the monoclonal antibody capable of simultaneously recognizing azilsartan and telmisartan is successfully prepared by taking candesartan as an immunogen and azilsartan as a coating antigen, the azilsartan and telmisartan are specifically detected, and the problem that the two drugs cannot be covered by the existing immunodetection technology is solved.

The application provides a fusion protein for detecting aquaporin 4 antibody and a preparation method and application thereof, and belongs to the technical field of aquaporin 4 antibody detection. The nucleotide sequence of the fusion protein is shown as SEQ ID NO. 5, the fusion protein is used as a coating antigen, and M1 type and M23 type aquaporin 4 can be simultaneously detected. The sensitivity and accuracy of the aquaporin 4 antibody detection are high, and the repeatability is good. When the kit of the application is used for aquaporin 4 antibody, the detection method is simple and rapid, and is suitable for large-scale screening of aquaporin 4 antibody in clinical and scientific research.

The invention provides a hybridoma cell strain secreting a toxoflavin monoclonal antibody. The hybridoma cell strain is obtained by immunogen immune animals prepared by coupling toxoflavin hapten and carrier protein. Wherein the carrier protein for preparing the immunogen comprises keyhole limpet hemocyanin KLH. The hybridoma cell strain is preserved in the Guangdong Microbial Culture Collection Center on March 10, 2026, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.67912. The invention also provides an application of the hybridoma cell strain, specifically, the toxoflavin monoclonal antibody secreted by the hybridoma cell strain is applied to toxoflavin detection. The kit further comprises a coating antigen, the coating antigen is prepared by coupling the toxoflavin hapten and carrier protein, and the carrier protein for preparing the coating antigen comprises bovine serum albumin (BSA). The monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity to toxoflavin, and can be accurately and reliably used for quantitative detection of toxoflavin in an actual sample.

The application belongs to the technical field of livestock pathogenic bacteria diagnosis, and particularly relates to a blocking ELISA kit and method for detecting Lawsonia intracellularis antibody. The kit comprises an LI-OmpA recombinant antigen peptide coated ELISA plate, an enzyme-labeled antibody, a positive control, a negative control, a sample diluent, a 20-fold concentrated washing solution, a substrate color developing solution and a termination solution. The method comprises using the LI-OmpA recombinant antigen peptide as a coating antigen and the enzyme-labeled LI-OmpA recombinant antigen peptide monoclonal antibody 5F2 as an enzyme-labeled antibody to establish the blocking ELISA method for the Lawsonia intracellularis antibody. The blocking ELISA kit for the Lawsonia intracellularis antibody provided by the application has the characteristics of high sensitivity and specificity, good repeatability and the like, and is suitable for the detection of high-throughput animal serum samples.

The application discloses a competitive ELISA detection method for Hib polysaccharide in an aluminum-containing adjuvant vaccine, and specifically, the method comprises the following steps: enzyme label plate preparation: coating Hib stock solution or PRP derivative on an enzyme label plate to prepare an enzyme label plate required for ELISA reaction; aluminum adsorption Hib sample treatment: blocking aluminum adjuvant adsorbed Hib reference and aluminum adjuvant containing vaccine samples with BSA, respectively, and then diluting the aluminum adjuvant adsorbed Hib reference and aluminum adjuvant combined vaccine samples after blocking; ELISA detection: adding the diluted aluminum adjuvant adsorbed Hib reference and aluminum adjuvant containing vaccine samples into the enzyme label plate, respectively, and then adding Hib specific antibody, generating ELISA signals through enzyme label antibody catalysis of substrates, establishing a mathematical relationship between absorbance and Hib polysaccharide content, and quantitatively calculating the Hib polysaccharide content in the aluminum adjuvant containing vaccine sample.

The application discloses a detection method and a kit for detecting Listeria monocytogenes, and relates to the technical field of biology. The kit is an indirect ELISA kit, and comprises an enzyme label plate coated with an antigen. The amino acid sequence of the antigen is shown as SEQ ID NO. 2. The indirect ELISA detection kit constructed in the application only reacts with positive serum of Listeria monocytogenes, does not cross-react with other serum, and has high specificity. When the serum dilution degree is 1:800, the detection result is still positive, and the sensitivity is high. The batch variation coefficient is between 2.79% and 6.64%, the batch-to-batch variation coefficient is between 2.61% and 7.75%, and the batch-to-batch repeatability is good. For 24 serum samples, the total coincidence rate is 95.83%. The kit provided in the application can effectively detect sample infection of Listeria monocytogenes, and has the advantages of high specificity, high sensitivity and good repeatability.

The invention discloses a double-genotype goose astrovirus multi-epitope fusion protein and a preparation method and application thereof, and relates to the technical field of chemistry. Screening of GAstV positive serum; designing and synthesizing a B cell epitope of the GAstV cap protein; screening of dominant B cell epitopes of the single genotype GAstV cap protein; expression and purification of the fusion epitope all-CAP; establishing a fusion epitope ELISA antibody detection method; detecting a clinical sample; and making a detection conclusion according to a detection result. According to the double-genotype goose astrovirus multi-epitope fusion protein as well as the preparation method and the application thereof, prepared GAstV single-genotype positive goose serum is used for screening to obtain cap protein B cell epitope polypeptide with good reactivity, and the fusion epitope polypeptide all-CAP is obtained after tandem expression and purification; a universal indirect ELISA antibody detection technology which is strong in specificity, high in sensitivity and good in repeatability and can be used for common detection of GAstV-1 and GAstV-2 is established by taking the GAstV-1 and GAstV-2 as a coating antigen.

The invention relates to an electrochemical luminescence immunosensor and application thereof in detection of DON, and belongs to the technical field of immunoassay. The electrochemical luminescence immunosensor comprises an electrochemical luminescence probe and an electrochemical luminescence electrode, the electrochemical luminescence probe comprises Eu-MOF, and the Eu-MOF is connected with a deoxynivalenol antibody through a chemical bond; the electrochemical luminescence electrode comprises an electrode body, the electrode body is modified with Au (at) Sn3O4, and the Au (at) Sn3O4 is connected with a deoxynivalenol coating antigen through a chemical bond. According to the present invention, the electrochemical immunosensor using Eu-MOF as the illuminant and potassium persulfate as the only co-reactant is developed, such that the purpose of sensitive detection of deoxynivalenol in cereals is achieved, and the important significance in food safety monitoring is provided. The sensor system is integrated, simple and convenient to control, rapid in trace amount, simple and convenient in pretreatment, high in sensitivity, wide in linear range, high in universality, controllable in cost and outstanding in practical value.

The invention discloses an electrochemical luminescence immunosensor for detecting ochratoxin A (OTA), and belongs to the technical field of biological detection. The sensor comprises an electrochemical luminescence probe and an electrochemical luminescence electrode, wherein the probe is prepared by coupling Tb-MOF subjected to carboxyl activation with an OTA antibody; the electrode is prepared by modifying Au-Ag NPs and then incubating with an OTA coating antigen. According to the invention, qualitative or quantitative analysis of OTA is realized by detecting the intensity of an electrochemical luminescence signal based on a competitive immunoreaction principle. The sensor has the advantages of being high in sensitivity, easy and convenient to operate, low in background signal, wide in linear range (0.0001-1000 ng / mL) and the like, is suitable for rapid detection of OTA in actual samples such as grains and feed, has the recovery rate of 92%-104%, and has good application prospects.

The application provides a colorimetric-fluorescent dual-mode determination method for hydrophobic anticancer drug curcumin, and the application realizes the preparation of oil amine branch joint polysuccinimide loaded curcumin (CUR@PSI OAm ) coated antigen, dual-mode probe labeled anti-PSI OAm antibody, and specific combination of antigen-antibody reaction for dual-mode ultra-trace detection and analysis of CUR@PSI OAm in cells, and indirect quantitative detection of hydrophobic anticancer drug curcumin in cells. Compared with the prior art, the dual-mode analysis method provided by the application can be mutually supported, and does not need to be enriched and preprocessed, is simple to operate, has short detection time, has the characteristics of high throughput, high sensitivity, high specificity, precise targeting and non-destructive detection, and can be used for ultra-trace non-destructive detection and analysis of hydrophobic anticancer drug curcumin (CUR) in cells.

The application provides a human-derived genetically engineered antibody with targeted simulation of Bt Cry1C toxin structure and insecticidal function and a high-activity mutant of the human-derived genetically engineered antibody, nucleotide sequences and amino acid sequences of the human-derived genetically engineered antibody are respectively shown as SEQ IN NO. 1 and SEQ IN NO. 2, nucleotide sequences and amino acid sequences of the mutant are respectively shown as SEQ IN NO. 3 and SEQ IN NO. 4; after the antibody and the mutant are expressed in prokaryotes, the LD50 of corresponding proteins to Plutella xylostella larvae is respectively 38.9% and 46.8% of the original Bt Cry1C toxin, the LD50 of corresponding proteins to cotton bollworm larvae is respectively 32.9% and 37.4% of the original Bt Cry1C toxin; the two can be respectively used as coating antigens in an IC-ELISA detection method to detect Bt Cry1C toxin with high sensitivity; the antibody and the mutant can be used as antigen analogue materials to replace the structure of Bt Cry1C toxin, not only can be used as new protein insect-resistant materials to have potential application value in green control of agricultural pests, but also can be used for detection of Bt Cry1C toxin residues in the environment and food samples, and have wide application value.