Method and special quantum dot fluorescent immunoassay kit for detecting quinolone compounds

A technology of quinolones and fluorescent immunity, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems such as human health hazards and ecological environment pollution, and achieve low cost, high sensitivity, and sample The effect of simple pre-processing

Active Publication Date: 2010-12-15
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the serious unreasonable use in animal husbandry, the potential threat of ecological envir

Method used

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  • Method and special quantum dot fluorescent immunoassay kit for detecting quinolone compounds
  • Method and special quantum dot fluorescent immunoassay kit for detecting quinolone compounds
  • Method and special quantum dot fluorescent immunoassay kit for detecting quinolone compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, preparation and detection method of quantum dot fluorescence immunoassay kit

[0039] 1. Quantum dot fluorescence immunoassay kit includes:

[0040] (1) Original coating solution: obtained by dissolving the original coating in the coating buffer, wherein the concentration of the original coating in the original coating solution is 0.08 μg / mL; the original coating is quinolone compound hapten and Bovine serum albumin conjugate;

[0041] (2) Quantum dot-labeled goat anti-mouse anti-antibody working solution:

[0042] It is obtained by diluting quantum dot-labeled goat anti-mouse anti-antibody with diluent, and the dilution ratio is 1:500;

[0043] The diluent is obtained by mixing 50mL bovine serum albumin and 950mL phosphate buffer; the concentration of the phosphate buffer is 0.02M, and the pH value is 7.4.

[0044] Goat anti-mouse anti-antibody was purchased from Beijing Boaosen, the product catalog number is bs-0295G.

[0045] (3) norfloxacin standard ...

Embodiment 2

[0099] Embodiment 2, test kit sensitivity, accuracy and shelf life test

[0100] 1. Kit sensitivity experiment

[0101] The zero standard solution (that is, the diluent is pH7.4, 0.05M phosphate buffer) was tested 20 times, and the average value of the measurement results plus 3 times the standard deviation was used as the minimum detection limit of the kit.

[0102] Table 1 Statistical table of zero standard measurement results μg / L

[0103]

[0104] It can be seen from Table 1 that the minimum detection limit of the kit is 0.5 μg / L.

[0105] 2. Standard product precision test:

[0106] From the three batches of kits described in Example 1 (batch 01, batch 02, and batch 03), 10 kits were extracted from each batch, and the luminous intensity value of the 4.5 μg / L standard solution was measured to calculate the coefficient of variation. The detection method is consistent with that described in Experiment 3 in Example 1.

[0107] The experiment was repeated three times, a...

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Abstract

The invention discloses a method and a special quantum dot fluorescent immunoassay kit for detecting quinolone compounds. The quantum dot fluorescent immunoassay kit for detecting the quinolone compounds provided by the invention comprises specific antibodies, coating antigens and standard solution of the quinolone compounds, wherein the coating antigens are conjugates of hapten and carrier proteins of the quinolone compounds. Experiments prove that the kit of the invention has the characteristics of simple sample pretreatment process, simple and convenient operation, low cost, high specificity, high sensitivity, high accuracy, on-site monitoring, suitability for screening of a large number of samples, and the like.

Description

technical field [0001] The invention relates to a method for detecting quinolone compounds and a special quantum dot fluorescent immunoassay kit thereof. Background technique [0002] At present, the problem of veterinary drug residues is one of the most important factors affecting the safety of animal food. Due to the complex composition of animal samples, the concentration of analytes is low, and most of the samples are small in volume, which poses a challenge to the selectivity and sensitivity of the analytical method. higher requirements. Quantum dots (Quantum dots, QDs) have been increasingly used in fluorescent immunoassays due to their unique optical and electrical properties. Traditional chromophores such as rhodamine 6G or other organic dye molecules have obvious advantages. The excitation spectrum of QD is wide and continuously distributed, while the emission spectrum is symmetrically distributed and narrow in width. The fluorescence emission wavelength can be adj...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/531C07K16/44C12N5/20C12R1/91
Inventor 吴聪明沈建忠张素霞王战辉史为民程林丽曹兴元汤树生
Owner CHINA AGRI UNIV
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