Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for quantitatively testing free triiodothyronine and preparation method thereof

A technology of thyronine and triiodothyronine, which is applied in the quantitative detection kit of free triiodothyronine. The effect of being quantified and reducing the impact

Active Publication Date: 2011-01-19
AUTOBIO DIAGNOSTICS CO LTD
View PDF0 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the binding ability of T3 and protein in plasma is weaker than that of T4, and the affinity constant with TBG is only 1 / 10 of that of T4

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for quantitatively testing free triiodothyronine and preparation method thereof
  • Kit for quantitatively testing free triiodothyronine and preparation method thereof
  • Kit for quantitatively testing free triiodothyronine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] The free triiodothyronine quantitative detection kit of the present invention comprises a suspension of magnetic particles coated with a triiodothyronine structural analog (diiodothyronine, T2)-gelatin, said The particle size of magnetic particles is 0.8-1.5um, and the surface contains carboxyl active groups with a concentration of 20-30ueq / g; free triiodothyronine prepared by adding pure triiodothyronine with hormone serum as the matrix Acid series calibrator; free triiodothyronine antibody labeled with horseradish peroxidase, using carbodiimide labeling method; composed of 0.2M Tris-Hcl, 0.15mM Luminol, 0.59mM hydroxycoumarin, 0.35mM gallic acid The luminescent substrate A solution composed of 0.2M acetic acid-acetate buffer solution, 0.85mM amino acid oxidase, 0.8% tween20, 0.5mMDTPA, 0.12mM vitamin C composed of luminescent substrate B solution and added with stabilizers and surface Concentrates of active agents in PBS.

[0039] The preparation method of the free t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for quantitatively testing free triiodothyronine, which comprises 3,3'-L-Diiodothyronine-gelatin enveloped magnetic bead suspension, a free triiodothyronine series calibrator, a horse radish peroxidase labeled free triiodothyronine antibody, chemiluminescent substrate A solution, chemiluminescent substrate B solution, and PBS buffer solution. The invention also discloses a method for preparing the kit. The kit has the advantages that: the chemiluminescence technology is combined with the immunomagnetic bead technology, the immunomagnetic beads are taken as a reaction carrier, the effective envelop amount of antigen is increased, raw materials are saved and the detection sensitivity and detection speed are obviously improved. A method for enveloping antigen analogs by the labeled antibody is adopted, so the analogs can be specifically combined with the antibody of hormone, but the combination capacity with thyroxine-binding protein is greatly reduced, and the influence of the binding protein on a measurement system is reduced to a large extent.

Description

technical field [0001] The invention relates to the field of biological immunoassay, in particular to a free triiodothyronine quantitative detection kit combining enzymatic chemiluminescence technology and magnetic separation technology, and also relates to a preparation method of the kit. Background technique [0002] The human thyroid gland can secrete thyroxine (T4) and triiodothyronine (T3) with physiological activity and anti-T3 without physiological activity. T4 in the plasma comes from the thyroid gland, and 80-90% of T3 and anti-T3 are produced by T4 in the peripheral tissue by removing an iodine atom. T3 and T4 in the blood exist in two forms, bound and free. The vast majority of thyroid hormones (T499.97%, T399.7%) are reversibly bound to plasma proteins, free thyroid hormones are in a very small amount in the blood, and protein-bound hormones and trace free hormones are in a dynamic balance , and it is these traces of free hormones that can enter the target tiss...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/78G01N33/577G01N33/535
Inventor 高晓丹陈晓玲刘保鑫付光宇渠海马建军项立红吴学炜苗拥军
Owner AUTOBIO DIAGNOSTICS CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com