Kit for quantitatively testing free triiodothyronine and preparation method thereof

A technology of thyronine and triiodothyronine, which is applied in the quantitative detection kit of free triiodothyronine. The effect of being quantified and reducing the impact

Active Publication Date: 2011-01-19
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the binding ability of T3 and protein in plasma is weaker than that of T4, and the affinity constant with TBG is only 1 / 10 of that of T4

Method used

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  • Kit for quantitatively testing free triiodothyronine and preparation method thereof
  • Kit for quantitatively testing free triiodothyronine and preparation method thereof
  • Kit for quantitatively testing free triiodothyronine and preparation method thereof

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Embodiment Construction

[0038] The free triiodothyronine quantitative detection kit of the present invention comprises a suspension of magnetic particles coated with a triiodothyronine structural analog (diiodothyronine, T2)-gelatin, said The particle size of magnetic particles is 0.8-1.5um, and the surface contains carboxyl active groups with a concentration of 20-30ueq / g; free triiodothyronine prepared by adding pure triiodothyronine with hormone serum as the matrix Acid series calibrator; free triiodothyronine antibody labeled with horseradish peroxidase, using carbodiimide labeling method; composed of 0.2M Tris-Hcl, 0.15mM Luminol, 0.59mM hydroxycoumarin, 0.35mM gallic acid The luminescent substrate A solution composed of 0.2M acetic acid-acetate buffer solution, 0.85mM amino acid oxidase, 0.8% tween20, 0.5mMDTPA, 0.12mM vitamin C composed of luminescent substrate B solution and added with stabilizers and surface Concentrates of active agents in PBS.

[0039] The preparation method of the free t...

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Abstract

The invention discloses a kit for quantitatively testing free triiodothyronine, which comprises 3,3'-L-Diiodothyronine-gelatin enveloped magnetic bead suspension, a free triiodothyronine series calibrator, a horse radish peroxidase labeled free triiodothyronine antibody, chemiluminescent substrate A solution, chemiluminescent substrate B solution, and PBS buffer solution. The invention also discloses a method for preparing the kit. The kit has the advantages that: the chemiluminescence technology is combined with the immunomagnetic bead technology, the immunomagnetic beads are taken as a reaction carrier, the effective envelop amount of antigen is increased, raw materials are saved and the detection sensitivity and detection speed are obviously improved. A method for enveloping antigen analogs by the labeled antibody is adopted, so the analogs can be specifically combined with the antibody of hormone, but the combination capacity with thyroxine-binding protein is greatly reduced, and the influence of the binding protein on a measurement system is reduced to a large extent.

Description

technical field [0001] The invention relates to the field of biological immunoassay, in particular to a free triiodothyronine quantitative detection kit combining enzymatic chemiluminescence technology and magnetic separation technology, and also relates to a preparation method of the kit. Background technique [0002] The human thyroid gland can secrete thyroxine (T4) and triiodothyronine (T3) with physiological activity and anti-T3 without physiological activity. T4 in the plasma comes from the thyroid gland, and 80-90% of T3 and anti-T3 are produced by T4 in the peripheral tissue by removing an iodine atom. T3 and T4 in the blood exist in two forms, bound and free. The vast majority of thyroid hormones (T499.97%, T399.7%) are reversibly bound to plasma proteins, free thyroid hormones are in a very small amount in the blood, and protein-bound hormones and trace free hormones are in a dynamic balance , and it is these traces of free hormones that can enter the target tiss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/78G01N33/577G01N33/535
Inventor 高晓丹陈晓玲刘保鑫付光宇渠海马建军项立红吴学炜苗拥军
Owner AUTOBIO DIAGNOSTICS CO LTD
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