51 results about "Human thyroid" patented technology

The invention relates to a quinoline multi-target kinase inhibitor with antitumor activity and a preparation method thereof. A general structural formula of the compound is shown in a formula (I) described in the specification. In vitro cell experiments verify that the compound provided by the invention has strong in vitro inhibitory activity on five common tumor cell lines, namely human thyroid carcinoma SW579, human hepatic carcinoma HepG2, human lung adenocarcinoma A549, human colorectal adenocarcinoma HCT116 and human gastric carcinoma MKN45, antitumor activities of most of target compounds are better than or equivalent to that of a positive control drug Cabozantinib, and the in vitro cell experiments verify that the compound provided by the invention has strong inhibitory activity on two kinases KDR and MET, so that the compound provided by the invention has a broad application prospect in preparation of a new antitumor drug.

The invention discloses a serum-free culture medium for efficiently expressing recombinant human thyroid-stimulating hormone in CHO cells and application thereof. The method comprises the steps that serum-free culture medium is used for conducting suspension cultivation on CHO cell strains in a bioreactor and efficiently expressing recombinant human thyroid-stimulating hormone in the CHO cells in a feed supplementation and batch cultivation mode. The invention further discloses a serum-free basic culture medium, a feed supplementation culture medium and a culture medium addition agent which are used in the recombinant human thyroid-stimulating hormone industrial production method. The serum-free culture medium has the main advantages that by means of the recombinant human thyroid-stimulating hormone industrial production method, the recombinant human thyroid-stimulating hormone can be efficiently produced, the product quality is good, safety is high, the expression quantity is large, the culture process is stable, the culture method is concise, the culture period is short, and the serum-free culture medium is suitable for mass production.

The invention discloses an enzyme-linked immunosorbent assay kit for quantitatively detecting a human-serum thyrotrophin receptor antibody and a detecting method. The kit contains a 96 ferment plate precoated with segment fusion protein at the carboxyl terminal of the extracellular domain of a recombinant human thyroid stimulating hormone receptor and the following bottled reagents: phosphate bleaching liquid, confining liquid, sample diluents, thyrotrophin receptor antibody standard liquid, goat-anti-human IgG second antibody labeled with horse radish peroxidase, chromogenic substrate (tetramethyl benzidine), reaction stopping solution and quality-control serum. The invention has the advantages that the detected human-serum thyrotrophin receptor antibodies give priority to a thyroid stimulation blocking antibody, the positive detection rate to patients with hashimoto thyroiditis is high, the detected result is accurate and objective, the method is simple and convenient, and the promotion and the application are easy. The invention has an important significance in diagnosis, guide therapy and curative effect evaluation of the autoimmune thyroid disorders, and has an important clinic reference value in plan making and method selection for treating Graves disease.

The invention relates to fusion proteins and bacteria encoding them. The fusion proteins include a ligand-binding domain interposed between the splicing domains of an intein. An auxotroph-relieving protein domain is fused to one of the splicing domains so that the auxotroph-relieving function of the domain is activated upon ligand binding. The fusion proteins can be expressed in bacterial cells and used as sensors of binding of compounds with the ligand-binding domain of proteins such as the human estrogen receptors or the human thyroid hormone receptor. The bacterially expressed fusion proteins can detect and report agonist and antagonist activity characteristic of the naturally-occurring hormone with the ability to modulate the function of the protein from which the ligand-binding domain of the fusion protein is derived.

The invention discloses a thyroid-stimulating hormone detection test paper, which comprises a plastic substrate, a sample pad and a water-absorbing pad are arranged on the plastic substrate, a protective film is arranged on the sample-loading pad and the water-absorbing pad, and the bottom between the sample-loading pad and the water-absorbing pad is A nitrocellulose membrane is provided, and the connection end of the sample pad is provided with solid-phase colloidal gold, and one end of the solid-phase colloidal gold is overlapped with one end of the nitrocellulose membrane, and the nitrocellulose membrane is sequentially coated with detection Line and quality control line; the manufacturing method steps include: preparation of nitrocellulose membrane; marking and solid phase of polyester fiber strip; assembly and cutting. The present invention applies the principle of double-antibody sandwich method and immunochromatography to qualitatively detect human thyroid-stimulating hormone (TSH) in urine. The detection steps are relatively simple, and the results can be observed within 10 minutes. General users can perform the detection by themselves. The present invention judges whether there is a risk of hypothyroidism by comparing the color depth of the detection line with the matched color card.

The invention relates to a preparation method of iron-modified cow dung-based biochar for adsorbing and removing perchlorates in water, and belongs to the field of applications of water treatment technologies. Perchlorates are toxic inorganic anions. The perchlorates in water can inhibit the ability of the human thyroid to absorb iodine and interfere the normal physiological functions of the thyroid, and are novel noteworthy pollutants in the water environment. Cow dung-based biochar prepared by pyrolysis in a nitrogen atmosphere is used as a matrix, and metal iron salts are coprecipitated onthe surface of the matrix to form iron-based biochar containg a large amount of hydroxyl groups. The material can efficiently remove the perchlorates in water by ion exchange in a solution, and solid-liquid separation can be quickly carried out by reduced pressure filtration or filtration. The raw material in the method is cow dung, and has the advantages of wide range of sources, low cost, and facilitation of environmental protection, and the regeneration process is simple, so the purpose of treating wastes with wastes is achieved.

Disclosed are DNA sequences encoding novel human thyroid peroxidase proteins and polypeptides capable of being secreted from cells, vectors comprising the sequences, and cells transformed with the vectors.

The invention discloses a vector and a preparation for knocking down a human CBS gene as well as a preparation method and application thereof, and relates to the technical field of biological pharmacy. The preparation method of the vector comprises the following steps: designing a primer for PCR amplification of a target gene according to a human CBS gene sequence, and the specific sequence is as shown in SEQ ID NO.1; restriction sites BamH I and Hind III in the GV102 vector are digested to form a linearized GV102 vector, and the sequence of the GV102 vector is hU6-MCS-CMV-GFP-SV40-Neomycin; and connecting the products obtained in the previous two steps to construct a GV102-CBS vector. The vector prepared by the invention can obviously inhibit the expression of human CBS genes, achieve the purposes of inhibiting the growth of human thyroid cancer cells and reducing the migration rate of the cancer cells, and can be used for preparing a preparation for treating the human thyroid cancer.

The invention discloses application of succinylation-related preparations for the regulation of mitochondrion OCR. According to the application of the succinylation-related preparations for the regulation of the mitochondrion OCR, mitochondrion oxygen consumption (p<average>is less than 0.05) is significantly reduced after treatment of human thyroid epithelial cells with palmitic acid, while the change of the mitochondrion oxygen consumption(p<average>is less than 0.05) is on the contrary after treatment of the human thyroid epithelial cells with palmitic acid and a desuccinylation inhibitor.The result of further detection of succinylation IDH2 (isocitrate dehydrogenase 2) shows that the result is achieved by regulating the succinylation of IDH2, that is, the succinylation of the IDH2 isreduced, mitochondrion OCR is reduced, and the desuccinylation inhibitor is involved in increasing or restoring succinylation of the IDH2, and the mitochondrion OCR is enabled to be increased or restored. The application of the succinylation-related preparations for the regulation of the mitochondria OCR provides a novel drug target for the treatment of congenital hypothyroidism.

The invention provides a traditional Chinese medicine composition for treating thyroid adenoma due to phlegm-damp stagnation and a preparation method thereof, and belongs to the field of traditional Chinese medicines. The composition is prepared from pinellia ternata, semen brassicae, kamuning, pericarpium citri reticulatae, codonopsis pilosula, rhizoma atractylodis macrocephalae, divaricate knotweed herb, Chinese lobelia, artemisia anomala and licorice root. The composition has the effects of invigorating spleen, replenishing qi, regulating flow of qi and middle jiao, drying dampness, removing phlegm, promoting blood circulation to remove stasis and resolving hard lump, can be used for effectively reducing the size of adenoma, has a remarkable curative effect against the thyroid adenoma due to phlegm-damp stagnation and limited influence on functions of human thyroid, and has small side effect.

The invention discloses an extraction-free detection kit and detection method for oncogene mutation in human thyroid glands, belonging to the technical field of molecular biology. According to the kit, a specific nucleic acid releasing agent and PCR reaction liquid matched with the specific nucleic acid releasing agent are adopted, a multiplex PCR capture technology and an NGS sequencing technology are cooperatively used, and the kit is used for qualitatively detecting various variations of BRAF, TERT, RET, TP53, NRAS, HRAS, KRAS and PIK3CA genes in a fresh tissue sample with uncertainty according to results of thyroid nodule needle biopsy cytology; and through detection of multiple genes and multiple sites and consideration of clinical pathological analysis results, assistance is providedfor auxiliary diagnosis and treatment of thyroid cancer.

The present invention relates to a composition for the diagnosis and treatment of recurrent thyroid cancer comprising recombinant human thyroid stimulating hormone (rhTSH) and a method for producing the recombinant human thyroid hormone. The method for producing recombinant human thyroid hormone according to the present invention can effectively produce rhTSH despite being cultured through fed-batch culture, and has a high purification yield and purity. Therefore, the recombinant thyroid stimulating hormone produced by the method can be usefully used for the diagnosis or treatment of recurrent thyroid cancer.

The invention relates to a peroxydase antibody plate type fluorescence enzyme lmmunoassay and the application thereof. The method is characterized in that human thyroid peroxydase (hTPO) protein is taken as antigens to cover a perforated plate, and PBST containing 1-5 percent BSA is used for sealing the perforated plate; the perforated plate is then taken as a carrier and is added with an anti-hTPO first antibody or a standard, an enzyme-labeled second antibody, fluorogenic substrate and stop solution in sequence; and finally, the fluorogenic strength is detected on a fluorogenic/chemical luminescent analyzer. The method can be used to clinically distinguish autoimmunity thyropathy from non-autoimmunity thyropathy and diagnosis lymphocytic thyroiditis. The invention has the advantages that the perforated plastic plate is taken as a solidoid carrier to carry out the fluorescence enzyme lmmunoassay, the plate material has easy sources, low cost and easy popularization; the method is special, sensitive and stable; the detection value is calibrated according to national standard with favorable accuracy and the method is applicable to clinically requirement and popularization.

The invention belongs to the technical field of biomedicine, and in particular relates to immune regulation polypeptide and application thereof. By modifying melittin, original instable amino acid and proline are replaced into positively charged lysine, thus enhancing stability; meanwhile, an active central part at N terminal is retained; antineoplastic polypeptide TT-1 with relatively high activity is obtained through a solid-phase chemical synthesis method. Result of a pharmacodynamic experiment proves that the TT-1 can inhibit growth of human thyroid carcinoma TT and tumor of mice with tumor, and the antineoplastic polypeptide is relatively low in toxicity, showing that the TT-1 can serve as a medicine for antineoplastic therapy.

The invention provides a human thyroid stimulating hormone receptor antibody quantitative detection kit. The human thyroid stimulating hormone receptor antibody quantitative detection kit comprises a solid phase carrier coated with an anti-TSHR monoclonal antibody A; an antigen reagent, wherein the antigen reagent is an immune complex of an anti-TSHR monoclonal antibody B and a TSHR; a human stimulated thyroid stimulating hormone receptor antibody marked by a marker is contained; the anti-TSHR monoclonal antibody A and the anti-TSHR monoclonal antibody B do not generate competitive binding. The kit provided by the invention adopts a competition method reaction principle to detect the concentration of the thyroid stimulating hormone receptor antibody in serum or plasma, the kit is relatively stable, the interference of biotin or fluorescein sodium can be avoided, the preparation process is simpler and more stable, the repeatability is good, and the cost is greatly reduced while the product performance is improved.

The invention relates to the technical field of biology, in particular to a kit for quantitatively detecting thyroid peroxidase and a non-diagnostic purpose detection method. The kit comprises a microwell plate, a buffer solution, a confining liquid, a sample diluent, an enzyme diluent, a thyroid peroxidase standard substance, a thyroid peroxidase antibody, a biological enzyme labeled thyroid peroxidase antibody and a luminous substrate, the sample diluent comprises a thyroid peroxidase antibody, PBST, newborn calf serum, a preservative and water. According to the invention, a double-antibodysandwich two-step method is established through an ELISA (Enzyme Linked Immuno Sorbent Assay) method, so that the content of human thyroid peroxidase can be conveniently, timely and accurately detected when the human thyroid peroxidase is researched, developed, recombined and naturally extracted, and the detection range is wide.

The invention discloses an application of pyrimidine derivatives to treatment of thyroid cancer. Thyroid cancer is an endocrine malignant tumor with high incidence. Cancer incidence and mortality datareleased by Chinese center for cancer control and prevention in 2018 show that about 170,000 new cases of thyroid cancer and about 8,000 deaths of thyroid cancer occurred in China in 2014. At present, drugs for effective treatment of thyroid cancer are few and cannot meet clinical needs. It is found that partial pyrimidine derivatives have significant inhibitory effect on human thyroid cancer B-CPAP cells and can be used for preparing drugs for treating the thyroid cancer. Parent nuclei and substituents of the pyrimidine derivatives significantly affect effect of the derivatives on thyroid cancer.

The invention relates to the technical field of biology, in particular to a human thyroid stimulating hormone receptor fusion protein and a preparation method and application thereof. The fusion protein is composed of a HER2 protein and a TSHR protein, and the HER2 protein and the TSHR protein are connected through a connecting peptide. The HER2 and the low-expression TSHR are subjected to fusionexpression, the yield and stability of the TSHR can be improved under the condition that the structure and function of the TSHR are not affected, the TSHR protein can be secreted into supernate, and purification is facilitated.

The invention discloses application of a pyrimidine derivative to treatment on thyroid cancer. The thyroid cancer is a high-incidence internal secretion malignant tumor; the data of incidence rate anddeath rate of cancers issued by National Cancer Prevention and Control Center of China in 2018 shows that about 170,000 thyroid cancer cases are newly increased in 2014, the number of people dying from the thyroid cancer is about 8,000; at present, few drugs for effectively treating the thyroid cancer are provided; the clinical needs cannot be met. The application of the pyrimidine derivative totreatment on thyroid cancer shows that part of the pyrimidine derivative has an obvious effect on inhibiting human thyroid cancer B-CPAP cells; the pyrimidine derivative can be used for preparing thedrugs for treating thyroid cancer; parent nucleus and substituent group of the pyrimidine derivative have an obvious influence on the drug effect on the thyroid cancer.