58 results about "Thyroid-stimulating hormone" patented technology

The invention relates to an aided diagnostic reagent of thyroid diseases and discloses a detection micro particle of thyroid stimulating hormone, which is a luminous micro particle coated by an antithyrotropic hormone antibody. The invention also discloses preparation and application of the thyroid stimulating hormone. In addition, the invention further discloses a vitro diagnostic kit of the thyroid stimulating hormone in a determined sample. Meanwhile, the invention discloses a using method of the kit. The kit can be combined with other serums and clinical information for aided diagnosis of the thyroid diseases and the monitoring of treatment effect of related patients.

The invention relates to the preparation of 131I-thyroid stimulating hormone (TSH) and an application thereof, belonging to the field of radionuclide therapeutic drugs. The compound 131I-thyroid stimulating hormone is a radioiodine marker for thyroid stimulating hormone with pathoclisis in vivo acting on thyrocyte. A preparation method of the 131I-thyroid stimulating hormone comprises the following steps of: carrying out iodine 131 marking on the thyroid stimulating hormone by utilizing a chloramine-T method, a peroxide oxidation method or an iodogen method, and introducing radionuclide iodine 131 for treatment into tyrosine residues in thyroid stimulating hormone molecules. The marking rate of the prepared 131I-TSH is 85.9 percent, the radiochemical purity after purification achieves more than 90 percent, and the prepared 131I-TSH can be stored stably for more than one week at room temperature; the 131I-TSH is mainly metabolized through the liver and excreted through the kidneys; the percentage of ID/g of the 131I-TSH in the thyroid gland is not obviously reduced in four hours; and the 131I-TSH can be concentrated in thyroid gland issues sealed by an iodine solution. Concerning low/undifferentiated thyroid tumours incapable of iodine uptake, the 131I-TSH can increase the radioiodine uptake of thyroid gland cancer cells, and the radioiodine 131 can be chosen for treatment.

Provided are methods for the detection and diagnosis of Hypothyroidism. The methods are based on the discovery that altered levels of selected analytes in sample fluid, typically blood samples, of patients are supportive of a diagnosis of Hypothyroidism. At least twenty-four new biomarkers for hypothyroidism are thus disclosed (singly or in any combination), Thyroid Stimulating Hormone, Interleukin-12p40, Tumor Necrosis Factor Alpha, Tissue Factor, Interleukin-15, Insulin, Immunoglobulin E, Growth Stimulating Hormone, Calcitonin, Prostate-Specific Antigen, Interleukin-4, Granulocyte Macrophage Colony Stimulating Factor, Matrix Metalloproteinase 9, Lymphotactin, Fatty Acid Binding Protein, Alpha Fetoprotein, Alpha-2 Macroglobulin, Serum Glutamic Oxaloacetic Transaminase, Matrix Metalloproteinase 3, Cancer Antigen 125, Mumps Antibody, Double Stranded DNA Antibody, Proliferating Cell Nuclear Antigen Antibody, Smith Antibody, or Herpes Simplex Virus 1 Glycoprotein D Antibody. Altogether the concentrations of one or more of these analytes, as well as Thyroid Stimulating Hormone, or any combination thereof, provide a sensitive and selective picture of the patient's condition, namely, whether the patient is suffering from Hypothyroidism. Kits containing reagents to assist in the analysis of fluid samples are also described.

The invention relates to the technical field of fluorescence immunochromatography in medical immunology and particularly relates to a parathyroid hormone determination kit and a preparation method. A test strip is arranged. The kit is characterized in that the test strip is sequentially provided with a PVC plate, a sample pad, a conjugate pad, a nitrocellulose membrane and an absorbent pad from bottom to top; a rare-earth Eu<3+> fluorescent microsphere-labelled thyroid parathyroid hormone monoclonal antibody is adsorbed to the conjugate pad; the diameters of rare-earth fluorescent microspheres are 200nm; the rare-earth fluorescent microspheres contain rare-earth lanthanide Eu<3+>, are stable in a ground state, and emit fluorescence with the wavelengths of 615nm under the action of an excitation light source of 337nm; and the monoclonal antibody is a purified and mixed monoclonal antibody, and is from monoclonal antibody cell lines of 2-6 different thyroid stimulating hormone epitopes. The kit has the advantages of being simple and convenient to operate, fast in reaction, high in sensitivity and high in specificity.

The invention discloses a thyroid-stimulating hormone detection test paper, which comprises a plastic substrate, a sample pad and a water-absorbing pad are arranged on the plastic substrate, a protective film is arranged on the sample-loading pad and the water-absorbing pad, and the bottom between the sample-loading pad and the water-absorbing pad is A nitrocellulose membrane is provided, and the connection end of the sample pad is provided with solid-phase colloidal gold, and one end of the solid-phase colloidal gold is overlapped with one end of the nitrocellulose membrane, and the nitrocellulose membrane is sequentially coated with detection Line and quality control line; the manufacturing method steps include: preparation of nitrocellulose membrane; marking and solid phase of polyester fiber strip; assembly and cutting. The present invention applies the principle of double-antibody sandwich method and immunochromatography to qualitatively detect human thyroid-stimulating hormone (TSH) in urine. The detection steps are relatively simple, and the results can be observed within 10 minutes. General users can perform the detection by themselves. The present invention judges whether there is a risk of hypothyroidism by comparing the color depth of the detection line with the matched color card.

The invention discloses a micro-fluidic chemiluminescence detecting system for magnetic particles for detecting thyroid functions. The system comprises a bottom plate and an upper chip, and the upperchip comprises a sample adding area in the center of the upper chip and five micro-fluidic reaction detecting channels communicated with the sample adding area, wherein the five micro-fluidic reactiondetecting channels include a total triiodothyronine micro-fluidic reaction detecting channel, a total thyroxine micro-fluidic reaction detecting channel, a free triiodothyronine micro-fluidic reaction detecting channel, a free thyroxine micro-fluidic reaction detecting channel and a thyroid stimulating hormone micro-fluidic reaction detecting channel. In application of the system, the bottom plate is arranged under the upper chip, and magnets which are permanent magnets or electromagnets are arranged at positions, corresponding to magnetic particle coating areas of the micro-fluidic reactiondetecting channels, of the bottom plate. By ingenious combination of the micro-fluidic technology and the magnetic particle chemiluminescence technology, quickness, high sensitivity and accuracy in quantitative detection of target substances are realized.

A method and kit for monitoring autoantibodies to thyroid stimulating hormone (TSH) receptor in a sample of body fluid, which employs the steps of:

• • (i) incubating TSH receptor with a sample of body fluid;
  • (ii) reacting the incubated sample of body fluid with at least one binding agent which is capable of binding to the TSH receptor in competitive reaction with TSH receptor autoantibodies (TRAb), or in a case where TSH receptor is complexed to labelled antibody, reacting the sample of body fluid with at least one binding agent which can bind to TRAb in such a way as not substantially to interfere with binding of the TRAb to the TSH receptor; and
  • (iii) detecting bound TRAb in the reacted incubated sample of body fluid.

An object of the present invention is to provide a method of high sensitive immunoassay using immunoagglutination reaction by antigen-antibody reaction for quantification of thyroid stimulating hormone (TSH). The present invention provides a method of assaying a thyroid stimulating hormone (TSH) comprising assaying agglutination which is generated by contacting TSH with a carrier to which an anti-TSH antibody has been bound, wherein a plurality of types of anti-TSH antibodies that recognize different epitopes of TSH are independently supported on separate carriers, and each carrier on which a TSH antibody has been supported is brought into contact with a TSH-containing analyte with time intervals.

The invention discloses an investigation and analysis method for abnormal thyroid function of healthy people in water-sourced high-iodine areas. 3218 healthy people were taken for physical examination, and fasting venous blood was collected to separate serum, and serum thyroid-stimulating hormone (TSH) was measured with an immunochemiluminescent instrument. , total triiodothyronine (TT3), total thyroxine (TT4), free triiodothyronine (FT3), and free thyroxine (FT4), and statistically analyzed the test results. The detection rate of thyroid disease is affected by many factors such as gender, age, race, and region. In areas with high iodine in water sources, the detection rate of abnormal thyroid function in healthy people increases year by year, mainly subclinical hypothyroidism, and is related to gender and Age-related, especially the higher incidence of subclinical hypothyroidism. Through the method of the invention, the thyroid function in an area can be screened and analyzed conveniently, the monitoring of thyroid diseases is strengthened, the early diagnosis of thyroid diseases is beneficial, and the health of the people is ensured.

The invention relates to low wash Thyroid Stimulating Hormone (TSH) immunoassays using an ELISA sandwich assay having limited or no wash step between the antigen capture, detection antibody addition and substrate introduction steps. This invention exhibits low cross reactivity with biologically similar interfering cross reacting species, such as Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Chorionic Gonadotropin (CG).

The invention discloses an intelligent detection method and system for thyroid stimulating hormone. The method comprises the following steps of acquiring identity recognition information of a detectedobject and a corresponding thyroid stimulating hormone detection result; analyzing the detection result to obtain an analysis report; and sending the analysis report to a terminal of the detected object and a medical care terminal. According to the intelligent detection method and system, voice information and identifier information containing identity information of the detected object are read;the identity information contained in a voice and the identity information contained in an identifier are compared; if the identity information contained in the voice is consistent with the identityinformation contained in the identifier, the thyroid stimulating hormone is detected; the detection result is analyzed; a detection value is compared with a preset threshold value to judge whether thedetection result is normal or not; and the detection result and abnormal alarm information are pushed to the terminal of the detected object and the medical care terminal to remind attention of a patient and medical care personnel, so that the consistency between the identity information of the detected object and a sample is ensured, the working efficiency is improved, and the convenience of theto-be-detected object is improved.

The invention discloses a thyroid stimulating hormone TSH kit based on a microfluidic chip and preparation and detection methods. The preparation method of the thyroid stimulating hormone TSH kit comprises the following steps: (1) coating the microfluidic chip; (2) labeling fluorescent microspheres; (3) assembling the microfluidic chip; (4) preparing a scaling product; (5) obtaining the thyroid stimulating hormone TSH kit based on the microfluidic chip. The prepared thyroid stimulating hormone TSH kit adopts a double-antibody sandwich method to carry out measurement, selects a time-resolutionfluorescent substance with high sensitivity as a labeling substance, carries out antibody labeling on the fluorescent microspheres and utilizes immunoreaction of antibody pairs to carry out analysis and detection, and the property of the prepared reagent can reach the level of an equivalent chemiluminescence reagent. After immunoreaction, cleaning solution is used for completely removing redundantcomponents, detection reading is carried out on the fluorescent microspheres combined by the immunoreaction, so that the problem that developing solution is introduced into the chip to cause incomplete reaction or untimely reading after developing is avoided.

The invention discloses an ovulation detection test strip. The ovulation detection test strip comprises a PVC bottom plate, a sample pad, a gold label pad, a nitrocellulose film and a water absorption pad, wherein the nitrocellulose film is provided with a detection line T1, a detection line T2 and a quality control line, the detection line T1 is coated with a mouse anti-human beta LH monoclonal antibody 1A4, the detection line T2 is coated with a cotinine antigen, and the quality control line is coated with a goat anti-chicken IgY polyclonal antibody; and a colloidal gold mouse anti-human beta LH monoclonal antibody 6B8 conjugate, a colloidal gold chicken IgY antibody conjugate and a colloidal gold cotinine monoclonal antibody conjugate are cured on the gold label pad. The test strip is high in detection sensitivity and good in specificity, abnormal interpretation results caused by crossing of LH with follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH) can be avoided, detection results are more accurate, and monitoring of the smoking metabolite cotinine in urine is combined. The invention further discloses an ovulation detection method, the steps are simple, and the test result is accurate.