Parathyroid hormone determination kit and preparation method

A parathyroid hormone and kit technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of strong interference and high intensity, and achieve the effects of high sensitivity, fast response, and clear clinical guidance

Inactive Publication Date: 2017-05-31
WEIHAI NEOPROBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the usual fluorescence measurement, because the test sample contains a variety of fluorescent components, the background fluorescence (scattered light caused by colloidal particles and solvent molecules in the sample and non-specific fluorescence emitted by proteins and other compounds in the serum) has a large intensity and interference. Strong, become the bottleneck of large-scale promotion of fluorescence analysis

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  • Parathyroid hormone determination kit and preparation method
  • Parathyroid hormone determination kit and preparation method
  • Parathyroid hormone determination kit and preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Each component of the test paper card in the parathyroid hormone (PTH) assay kit (fluorescence immunochromatography) can be made by the following measures:

[0044] 1. Preparation of sample pad 2:

[0045] Soak the glass fiber membrane in the treatment solution containing 1.0% Triton X-100, 2.5% BSA, 0.15M Tris buffer, pH7.5, soak at 4°C for 4 hours, then place it in an oven and dry it at 37°C 2 hours.

[0046] 2. Preparation of binding pad 3 for absorbing fluorescent microsphere-labeled antibody:

[0047] Soak the glass fiber membrane in 150mM Tris-HCL treatment solution (containing 1.0% Triton X-100, 2.5% BSA, pH7.4), soak for 2 hours at 4°C, then take it out of the oven at 37°C and dry it for 4 hours, and set aside. The glass fiber membrane was placed on the Bio-DotXYZ3050 three-dimensional spraying platform, and the rare earth Eu 3+ The conjugated complex of anti-parathyroid hormone monoclonal antibody labeled with fluorescent microspheres is sprayed onto the gla...

Embodiment 2

[0056] Embodiment 2: accuracy test

[0057] Select the above test paper card and fluorescence immunochromatography analyzer (model: NEO-007),

[0058] Setting of the parameters of the fluorescence immunoassay analyzer: After setting the process parameters of the test paper card on the fluorescence immunoassay analyzer, take the above assembled test paper card and use 10, 100, 200, 500, 600, 800, 1000pg / mL respectively The parathyroid hormone calibrator is measured with a test paper card to obtain the fluorescence intensity value of each calibrator, and the result is input into the parameters of the analyzer to complete the parameter setting of the analyzer.

[0059] Main testing materials: clinical samples were obtained from relevant hospitals, a total of 300 latex-enhanced immunoturbidimetric value samples, including 100 serum samples, 100 plasma samples, 100 whole blood samples, and the distribution range of parathyroid hormone content was 10.0- Between 1000pg / mL.

[0060]...

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Abstract

The invention relates to the technical field of fluorescence immunochromatography in medical immunology and particularly relates to a parathyroid hormone determination kit and a preparation method. A test strip is arranged. The kit is characterized in that the test strip is sequentially provided with a PVC plate, a sample pad, a conjugate pad, a nitrocellulose membrane and an absorbent pad from bottom to top; a rare-earth Eu<3+> fluorescent microsphere-labelled thyroid parathyroid hormone monoclonal antibody is adsorbed to the conjugate pad; the diameters of rare-earth fluorescent microspheres are 200nm; the rare-earth fluorescent microspheres contain rare-earth lanthanide Eu<3+>, are stable in a ground state, and emit fluorescence with the wavelengths of 615nm under the action of an excitation light source of 337nm; and the monoclonal antibody is a purified and mixed monoclonal antibody, and is from monoclonal antibody cell lines of 2-6 different thyroid stimulating hormone epitopes. The kit has the advantages of being simple and convenient to operate, fast in reaction, high in sensitivity and high in specificity.

Description

[0001] Technical field: [0002] The invention relates to the technical field of fluorescence immunochromatography in medical immunology, including protein cross-linking technology, membrane chromatography technology, marker immunoassay technology and the like. Specifically, it is a parathyroid hormone assay kit capable of quickly and accurately performing quantitative analysis of parathyroid hormone in samples such as serum, plasma, and whole blood, and a production method. [0003] Background technique: [0004] Parathyroid hormone (PTH) is one of the important hormones synthesized in the parathyroid gland and secreted into the blood to maintain calcium balance in the body. It directly acts on the bones and kidneys to promote bone calcium mobilization and kidney reabsorption of calcium , by promoting 1α hydroxylase to convert 25-OH-D3 into active 1,25-(OH)2D3, which indirectly plays the role of enhancing intestinal calcium absorption. PTH not only decomposes bone, but also s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/558G01N33/577
CPCG01N33/558G01N33/533G01N33/577
Inventor 孙宁宁李红江陈萍萍
Owner WEIHAI NEOPROBIO
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