11results about How to "Suitable for detection" patented technology

This invention discloses a stable transfected cell line for glucocorticoid detection based on the Gal4-DBD-GRLBD fusion protein of marine medaka and its construction method, belonging to the field of environmental toxicology detection technology. The construction method includes: designing specific primers to amplify the GR ligand-binding domain (LBD) of marine medaka, ligating it to the pBIND vector to form the coding sequence of the Gal4-DBD-GRLBD fusion protein, subcloning it into the Dualpromoter vector to construct the pBIND-omeGR expression plasmid; co-transfecting HeLa cells with the pBIND-omeGR plasmid, UAS reporter plasmid, and transposase plasmid at a mass ratio of 1.66:1:0.34, and obtaining the stable cell line HeLa-pBIND-omeGR expressing the fusion protein after selection with puromycin. The cell line exhibits ECMO activity against dexamethasone. 50 The value was 0.841 nM, and the induction activation factor was 2.34 times. This invention solves the technical problems of existing mammalian GR detection systems, which are difficult to accurately predict fish toxicity effects and have complex construction of full-length receptor systems. It has the advantages of simple construction, strong versatility, and good stability, and can be used for marine ecotoxicological assessment, environmental pollutant monitoring, and chemical safety evaluation.

The utility model discloses a movable bearing acoustic detection device. The detection device comprises a box body, a data processing structure and a collector, trundles are arranged at the bottom of the box body, and a detachable box cover is arranged on the side face or the top of the box body. The data processing structure comprises an amplifier, an acquisition board and an industrial personal computer, and is integrated and fixed in the box body; the collector is detachably arranged in the box body, is separated from the box body and is taken out during detection, and the collector is electrically connected with the amplifier, the collection plate and the industrial personal computer in sequence. The utility model has the advantages of safety, durability, convenience in operation, flexibility and portability, breaks through the limitation of the original fixing equipment, and is suitable for various environments and occasions.

The invention provides a construction method and application of an SERS (Surface Enhanced Raman Scattering) biosensor for detecting vomitoxin. The SERS biosensor is used for rapidly detecting the vomitoxin (DON) in real time. Ag is sputtered on the surface of the filter paper through a magnetron sputtering method, so that the surface of the filter paper has a Raman signal enhancement effect. An aptamer (Apt) of vomitoxin is selected as a recognition sensing element to specifically capture DON, Apt is connected to filter paper through an Ag-S bond, Au-4-ATP-cDNA is further modified and connected to the filter paper through complementation of Apt and cDNA, and 4-ATP serves as a signal molecule to provide a Raman signal. When the DON exists, the Apt is combined with the DON and is separated from the cDNA, the Raman signal of the 4-ATP is weakened after the Au-4-ATP-cDNA falls off, and the DON is quantitatively analyzed by detecting the Raman signal change of the 4-ATP. The sensor has high sensitivity, specificity and stability, provides a new idea for on-site rapid detection and real-time monitoring, and has application value and application prospect.

The application discloses a plasma metabolic marker composition and a screening method and application thereof, and relates to the technical field of biomedicine. The plasma metabolic marker composition comprises glycyl-L-glutamic acid, phosphatidylcholine 38:7, pyrrole-2-formic acid and phosphatidylethanolamine 36:2p. The metabolic marker composition is significantly different in different cancer types, can distinguish different cancer types of cancer patients, and realizes the detection of pan-cancer. The metabolic marker composition is non-invasive and minimally invasive when used for the detection of pan-cancer, is more suitable for the screening and detection of a large number of ordinary people, has high sensitivity and specificity, is more suitable for the early screening of cancer, and is helpful for the early screening, early diagnosis and early treatment of cancer, and is helpful for improving the survival rate and cure rate of cancer patients.

The application discloses a kind of potassium ion sensing test paper, potassium ion concentration detection device, system and detection method.Calibration-free potassium ion sensing test paper includes: sample chamber, when it is contacted with sample, can be automatically absorbed into sample by capillary force to complete sample collection and filling;Detection electrode is used to carry out electrochemical detection to the sample collected in thin layer chamber.The calibration-free potassium ion sensing test paper of thin layer coulomb method of the application can calculate the potassium ion concentration in sample according to the charge quantity change caused by potassium ion transfer combined with the volume of thin layer sample, and can be used for non-invasive detection of potassium ion concentration in body fluid, the detection result is not disturbed by temperature change, high accuracy, and test paper can be reused, without pre-measurement calibration curve.

The invention discloses a portable pipeline blockage point rapid positioning instrument and method based on pressure transient analysis, the positioning instrument comprises a positioning instrument host, a butt joint of a three-way structure, a high-speed electromagnetic valve and a high-frequency pressure sensor, the high-speed electromagnetic valve and the high-frequency pressure sensor are integrated in the butt joint; and signal and power supply connection with the host is realized through the quick connector. The positioning method comprises the following steps: connecting equipment and recovering a signal; the host controls the high-speed solenoid valve to instantly open and close to generate pressure pulse; the high-frequency pressure sensor collects incident and blocking point reflection pulse signals; the host preprocesses the data through wavelet transform, cross-correlation analysis and other algorithms and calculates a pulse time difference delta t; and calculating and displaying the distance of the blocking point according to a formula S = (wave velocity a * delta t) / 2, wherein the wave velocity can be calibrated through a self-adaptive algorithm. The positioning precision reaches the meter level, equipment is portable, operation is easy and convenient, point distribution and excavation in advance are not needed, the method is suitable for multi-material pipelines, and the detection cost and the technical threshold are reduced.

This invention belongs to the field of pharmaceutical technology and relates to drug identification and detection methods, specifically to probe primers and their applications in the identification of *Pheretima aspergillum* and PCR detection methods. The probe primers consist of the sequences shown in SEQ ID NO. 1-8 and SEQ ID NO. 9-17; these probe primers are used for the specific and quantitative detection of *Pheretima aspergillum* using quantitative real-time PCR methods (including real-time quantitative PCR and digital PCR). The detection method provided by this invention can effectively detect *Pheretima aspergillum*, including newly discovered subgroups, exhibiting strong specificity and high sensitivity. It can be used for the qualitative or quantitative detection of *Pheretima aspergillum* in various forms of traditional Chinese medicine, including raw materials, processed slices, granules, decoctions, and compound preparations, demonstrating strong innovation and good application value.

The application provides a blanking hole clogging detection system based on infrared thermal imaging. The blanking hole is adjacent to the end of a material conveying belt. The thermal imaging device of the infrared image acquisition unit comprises a long-wave infrared detector arranged at the blanking hole through a mounting bracket. The long-wave infrared detector can obtain a continuous infrared image sequence in a high-concentration dust environment. The field angle of the long-wave infrared detector is adjusted to cover the entire blanking hole area, so that an abnormal heat area formed when the blanking hole is clogged enters the imaging range completely. When the detection system is working, the intelligent computing unit analyzes the infrared thermal image sequence, analyzes the spatial distribution characteristics and time evolution characteristics of the temperature field, and determines whether the blanking hole is clogged by using a multi-feature fusion diagnosis model. The application can accurately and continuously collect and continuously monitor the infrared light data at the blanking hole in a high-dust-concentration environment and under the condition that the infrared light characteristics of different materials of the conveying belt are seriously different, and determine whether the blanking hole is clogged abnormally.

The utility model discloses a flood control detection early warning device which comprises a base and a top plate, a measuring assembly is installed on the base, the measuring assembly comprises a measuring cylinder and scale marks, a floating assembly is arranged on the inner side of the measuring cylinder, the floating assembly comprises a floating block and a roller, an observation assembly is installed on the inner side of the measuring cylinder, and the observation assembly is connected with the top plate. The observation assembly comprises a camera and an observation port, the measuring cylinder is provided with an early warning assembly, the early warning assembly comprises a movable plate and a button, and the top plate is provided with a lifting assembly. According to the flood prevention detection early warning device, waves on the water surface cannot be conducted to the inner side of the measuring cylinder, and the stability of vertical movement of the floating block is guaranteed; the water level detection accuracy is guaranteed, then the reliability of early warning work can be guaranteed, the early warning value of the water level depth can be conveniently and rapidly adjusted according to the condition of an embankment, the adaptability of early warning and monitoring work of a water body is improved, the safety of early warning and detection of the water body is improved, and the method is suitable for detection and early warning of the water level of the water body.

The invention discloses a structural design and processing technology of a high aspect ratio micro gas chromatographic column based on a hot reflux technology, and belongs to the technical field of glass processing. The silicon-glass high-aspect-ratio micro gas chromatographic column cannot be obtained by anodic bonding; and the problems of difficulty in preparation of a high-aspect-ratio structure, poor surface quality and stress concentration in existing glass micromachining are solved. According to the invention, efficient preparation of the high aspect ratio microstructure of the glass is realized, no obvious defect exists on the surface, and stress and cracks are effectively inhibited; according to the structural design of the miniature gas chromatographic column, the snakelike layout and the optimized depth-to-width ratio design are adopted, so that the separation efficiency is improved, a foundation is laid for high theoretical plate number and excellent separation degree, and the rapid separation requirement is met; the processing technology is stable, the silicon-glass bonding airtightness is good, and the method is suitable for on-site detection and on-line monitoring scenes; and batch preparation can be realized by the wafer-level process, the cost is reduced, and industrial application is facilitated.