39 results about "PHERETIMA ASPERGILLUM" patented technology

The invention discloses a method for identifying a DNA bar code molecule of an earthworm and a COI sequence of the DNA bar code molecule of the earthworm. The method comprises the steps of amplifying the gene of a sample COI through polymerase chain reaction (PCR), verifying the PCR product by agarose gel, then sending to a biological sequencing company for sequencing, according to the sequencing result, sequence splicing through manual correcting, comparing with a public sequence, then confirming the tissue to be tested as the source of pheretima aspergillum or pheretima vulgaris if the homology of the spliced sequence and the gene sequence SEQ ID NO.1-NO.4 is over 99%. By adopting the reliable DNA molecular identification technology, the method can rapidly and accurately identify the earthworm varieties specified by the 'pharmacopoeia of China' and non-medicinal material earthworm varieties without conducting morphological identification on the earthworm varieties, can further distinguish the pheretima aspergillum varieties from pheretima vulgaris varieties, improves the accuracy and reliability, and guarantees the safety of the earthworm as the medicinal material.

The invention discloses a method for identifying dried Pheretima aspergillum based on a PCR-RFLP technology. Universal primers of a COI gene are used to carry out PCR amplification to obtain mitochondrial cytochrome oxidase (COI) gene fragments of all kinds of earthworms, the sequences of the fragments are sequenced and compared, the complete sequences of the COI fragments of the different kinds of earthworms undergo restriction enzyme map analysis by DNAMAN software to finally screen a restriction enzyme RsaI, the restriction enzyme RsaI is used to carry out enzyme digestion on the above obtained PCR product, the enzyme digestion result of the PCR product is detected by 2% agarose gel, and the enzyme digestion maps are used to identify the dried Pheretima aspergillum and other varieties.The method is simple and reliable to operate, and provides a new technical way for the identification of dried Pheretima aspergillum products.

The invention provides a traditional Chinese medicine formula with an obvious curative effect on cerebral ischemic stroke such as cerebral thrombosis, cerebral infarction and the like. The traditional Chinese medicine formula comprises the following traditional Chinese medicines in parts by mass: 50-150g of raw radix astragali, 3-6g of scorpio, 1-3g of centipede, 6-9g of periostracum cicada, 10-25g of pheretima aspergillum, 9-20g of Chinese polyphaga, 6-10g of leech, 5-10g of gadfly, 5-10g of agkistrodon, 10-30g of rhizoma gastrodiae, 10-15g of radix polygalae, 9-15g of fructus schisandrae chinensis, 9-15g of spina date seed, 10-16g of fructus alpiniae oxyphyllae and other adjuvant drugs. The traditional Chinese medicine formula provided by the invention is suitable for treating cerebral ischemic stroke.

The invention discloses a traditional Chinese medicine composition and application thereof in the preparation of a drug for treating chronic obstructive pulmonary disease. The traditional Chinese medicine composition is prepared from the following raw materials in part by weight: 1 part of herba epimedii, 1-5 parts of radix codonopsitis, 1-5 parts of radix astragali, 1-5 parts of radix salviae miltiorrhizae, 1-5 parts of radix angelicae sinensis, 1-5 parts of radix rehmanniae preparata, 1-5 parts of radix ophiopogonis, 1-5 parts of pheretima aspergillum, 1-5 parts of radix platycodi and 1-5 parts of radix glycyrrhizae. According to the invention, the traditional Chinese medicine composition can effectively prevent pulmonary angiogenesis and remodeling and effectively reduce airway remodeling; the long-term clinical experience proves that the traditional Chinese medicine composition has good effects of preventing cough, eliminating phlegm, resisting inflammation, preventing emphysema and pulmonary heart disease, improving physical fitness and quality of life, and has no throat discomfort, hoarseness, oropharyngeal candidiasis, rash, contact dermatitis and other side effects.

The invention discloses a compound feed low in carbon for adult anguilla rostrata. The compound feed comprises the following components in parts by weight: 15-35 parts of hermetia illucens powder, 10-15 parts of clamworm powder, 10-15 parts of powder of a mixture of one or more of pheretima aspergillum(E.Perrier),pheretima guillelmi (Michaelsen),pheretima vulgaris Clen and pheretima pectinifera Michaelsen, 4-8 parts of powder of a mixture of one or more of achatina fulica, helix aspersa and cathaica, 4-8 parts of imported white fish powder, 3-5 parts of fly larva powder, 3-5 parts of bombyx mori linnaeus powder, 3-5 parts of yellow mealworm powder, 3-5 parts of vital wheat gluten, 3-5 parts of whey powder, 2-4 parts of sweet potato flour, 2-4 parts of corn flour, 2-4 parts of strong flour,2-4 parts of soybean flour, 1-2 parts of entermorpha dried marine algae powder, 1 part of yeast powder, 1 part of soybean lecithin, 1 part of multiple vitamins, 1 part of multiple minerals, 0.2-0.4 part of methionine, 0.2-0.4 part of glutamic acid and 0.1-0.2 part of choline. Worm protein is used for replacing fish meal protein, so that the cost is reduced, the mouth feel of the adult anguilla rostrata can also be improved, the taste bud of the adult anguilla rostrata can be stimulated, and the growth rate of the adult anguilla rostrata is increased; and the worm protein also has the effect of improving immunity, and besides, the substances of marine algae polysaccharide and the like are added, so that the corresponding disease resistance and the inverse resistance are achieved, and further the disease resistance of the adult anguilla rostrata is strengthened, and the survival rate of the adult anguilla rostrata is increased.

This invention relates to a Chinese medicine capsule for treating the pain resulted from lumber vertebrae disease and cervical spondylosis, ischium sciatica, apoplexia, hemiplegic paralysis, myophagism and got, etc., which is prepared from Ephedra sinical stapf, pheretima aspergillum perri, pyrola notundifolia L, Radix Ledebourielolae and Resina Commiphorae etc. through the process of respectively disintegrating, mixing and screening (120 mesh), fully uniformly mixing and drying at 50 degree C for 1-3 hours to obtain a brown powder to be made into capsule. Said medicine has the functions of dispelling wind, promoting blood-circulation, relieving pain and activating meridians and collaterals and has the advantages of obvious effect, long active effect, easy-to-absorb and free from any toxic and side effect.

The invention discloses a multifunctional non-woven fabric production process, which comprises the following steps: adding prepared angelica sinensis powder, incised notopterygium powder, rhizoma Ligustici powder, radix aconiti preparata powder, black radix aconiti lateralis praeparata slice powder, rhizoma chuanxiong powder, radix paeoniae rubra powder, flos carthami powder, pheretima aspergillum powder, resina draconis powder, calamendiol powder, juncus pith powder, herba asari powder, cassia twig powder, radix salviae miltiorrhizae powder, saposhnicovia divaricata powder, semen raphani powder, radix clematidis powder, olibanum powder, myrrh powder and borneol powder into a spinning solution of viscose, and uniformly stirring by a stirrer so as to obtain a mixed spinning solution; and carrying out wet spinning on the mixed spinning solution so as to obtain multifunctional viscose. When in use, compositions of the medicines above are volatilized by heat of the human body and then inhaled from the nostrils, the medicines are coordinated and compatible, and mutually gain, so that the effects of clearing and activating the channels and collaterals, dispelling wind and settling pains are achieved.

The invention discloses a group of earthworm characteristic polypeptides and application thereof in species identification of earthworm medicinal materials. The five characteristic polypeptides have good specificity and stability, can be stably detected in earthworm samples of corresponding species, and cannot be detected in non-corresponding species of earthworms including pheretima guillelmi, pheretima californicum, pheretima aspergillum, pheretima fargesii and other species. The five characteristic polypeptides have excellent representativeness, can distinguish most of earthworm medicinal material species in the market, have the certified product identification rate of 96%, and have good application prospects.

The invention discloses a nutrient substrate for pheretima aspergillum as well as a preparation method and a culture method of the pheretima aspergillum. The nutrient substrate for the pheretima aspergillum comprises a fermented product obtained by fermentation of plant extract residues, the C/N of the fermented product is 20:(1-30):1, the organic matter content is 30%-40%, the electrical conductivity is 1754-1906S*cm<-1>, and the humidity is 30%-55%. According to the invention, a single or mixed waste residues obtained after active ingredients are extracted in a plant extraction factory are used as raw materials, and beneficial microorganisms are utilized for aerobic micro-fermentation treatment, so that the culture substrate with a suitable C/N value, enriched organic matter and suitableelectrical conductivity is obtained; and the pheretima aspergillum is cultured indoors, the culture environments are strictly controlled, the culture process is standardized, and therefore the pheretima aspergillum has robust and strong growth, high reproductive capacity, and good vitality.

The invention discloses a medicine for treating rheumatoid arthritis. The medicine is characterized by comprising raw materials in parts by weight as follows: 10-12 parts of frankincense, 10-12 parts of prepared myrrh, 10-12 parts of prepared common monkshood mother roots, 10-12 parts of earthworms, 10-12 parts of ground beetles, 10-12 parts of peach seeds, 1-2 parts of centipedes, 10-12 parts of orientvine stems, 10-12 parts of coix seeds, 10-12 parts of fresh rehmannia roots, 10-12 parts of chinaroot greenbier rhizomes, 10-12 parts of pheretima aspergillum, 10-12 parts of milkvetch roots, 10-12 parts of cassia twigs, 10-12 parts of kadsura pepper stems, 10-12 parts of Chinese clematis rootsand 10-12 parts of liquorice roots. The medicine has the advantages that the raw materials are easy to obtain, the cost is low, the treatment effect is significant and the like.

The invention discloses a plaster for treating pain caused by a cancer. The plaster is prepared from the following raw herbal materials: Herba Hedyotidis Diffusae 130 parts, Spina Gleditsiae 60 parts, Rhizoma Sparganii 70 parts, Rhizoma Curcumae 70 parts, Rhizoma Chuanxiong 70 parts, Herba Solani Lyrati 130 parts, Herba Scutellariae Barbatae 120 parts, Pheretima aspergillum 70 parts, Zaocys 70 parts, Rhizoma Paridis 90 parts, Semen Strychni 50 parts, Radix Aconiti 50 parts, Radix Kansui 50 parts, Olibanum 50 parts, Sanguis Draxonis 40 parts, Radix Notoginseng 40 parts, Flos Caryophylli 40 parts, Sal Ammoniacus 40 parts and Scolopendra 40 parts. The plaster can effectively relieve pain caused by the cancer. During treatment, the plaster is applied to a focus of infection and acupuncture points such as the Shenque acupoint, Feishu acupoint, Huagai acupoint, Tanzhong acupoint and Dashu acupoint, and is changed every seven days. For a patient suffering from a terminal cancer, pain of the patient can be relieved after 8-24 hours if the patient uses the plaster. The plaster not only can change mental outlook of the patient, but also brings various conveniences and spiritual comfort for relatives of the patient.

The invention discloses a Naosaitong pill and a preparation method thereof. The preparation method is characterized by comprising the following steps: taking 21.5 g of piper kadsura, 43 g of xanthium sibiricum fruit, 10.75 g of litsea cubeba fruit, 35 g of apocynum venetum leaf, 10.75 g of nelumbo nucifera seed, 17.15 g of astragalus membranaceus root, 21.75 g of achyranthes bidentata root, 30 g of trichosanthes kirilowii root, 16 g of paeonia suffruticosa bark, 21.5 g of prunus persica seed, 14.25 g of sodium sulfate powder, 10.75 g of ligusticum chuanriong root, 14.25 g of lepidium apetalum seed, 18.75 g of pheretima aspergillum (fried), 64.5 g of rehmannia glutinosa root, 10.75 g of hirudo nipponica (boiled), 7 g of cinnamomum cassia bark and 28.55 g of poria cocos for crushing into coarse powder, putting the coarse powder into a circulating ultrasonic extractor, adding water, germanium powder and Tween 60, conducting ultrasonic extraction, conducting jet flow spray drying, preparing nanometer extract powder, and preparing the pill with refine honey so as to obtain the Naosaitong pill. The Naosaitong pill is used for treating cerebral thrombosis, cerebral heamorrhage sequelae, paralytic limbs, deadlimb and aphasis, has an obviously better curative effect than the commercially available Naosaitong pill, and achieves good effects.

The embodiment of the invention provides a primer pair, a method for identifying pheretima asperillum by using the primer pair, and a kit for identifying the pheretima asperillum. By adopting the primer pair and the method provided by the embodiment of the invention, the DNA fragment in the pheretima asperillum can be specifically amplified, so that the pheretima asperillum can be accurately identified.

The invention discloses a traditional Chinese medicinal composition for treating chronic colitis and a preparation method thereof. The traditional Chinese medicinal composition is prepared from the following components in parts by weight: 8 to 10 parts of radix astragali, 6 to 8 parts of salviae miltiorrhizae, 5 to 7 parts of radix scrophulariae, 3 to 5 parts of radix notoginseng, 8 to 10 parts of radix rehmanniae preparata, 6 to 8 parts of parched white peony roots, 2 to 4 parts of buthus martensi karsch, 6 to 8 parts of lonicerae japonicae flos, 5 to 7 parts of roasted rhizoma atractylodis macrocephalae, 6 to 8 parts of radix cyathulae, 5 to 7 parts of fructus chebulae pulp, 4 to 6 parts of dried ginger, 5 to 7 parts of pheretima aspergillum, 6 to 8 parts of rhizoma chuanxiong, 4 to 6 parts of Chinese polyphaga, 3 to 5 parts of cortex magnoliae officinalis, 5 to 7 parts of rhizome pinelliae preparata, 6 to 8 parts of poria cocos, 5 to 7 parts of radix paeoniae rubra, 5 to 6 parts of horttuyia cordala thunb polysaccharide, 9 to 10 parts of lactose, 15 to 18 parts of microcrystalline cellulose, and 6 to 8 parts of CaHPO4. The traditional Chinese medicinal composition for treating the chronic colitis disclosed by the invention has bi-directional adjustment multi-level effect on a human body, integrates the functions of balancing yin-yang of the human body, regulating qi and blood, tonifying qi, strengthening the foundation, smoothing stasis blocking, purifying blood, detoxifying, dispelling phlegm, transforming turbidity and the like, has a clear treatment effect on treating the chronic colitis, and has a stable and lasting effect.

The invention discloses a phlegm-reducing and asthma-relieving tea. The phlegm-reducing and asthma-relieving tea is prepared from 2 to 5% of Flos Farfarae, 8 to 16% of Rhododendron dahuricum, 5 to 8% of Pheretima aspergillum, 5 to 8% of tatarian aster root and 5 to 8% of tea leaves. A preparation method for the phlegm-reducing and asthma-relieving tea comprises the following steps: careful selection of raw materials in proportion; uniform mixing; cleaning; crushing; drying; filling a non-woven fabric inner bag with the raw materials; filling an aluminum foil outer bag with the inner bag; and hot pressing and sealing.

The invention belongs to the field of animal feed additives and especially relates to an animal feed additive prepared by the utilization of amino acid in Pheretima aspergillum and a preparation method thereof. The animal feed additive is characterized by comprising the following ingredients (by weight): 10-60 parts of a Pheretima aspergillum amino acid solution containing 90% of water, 5-30 parts of earthworm powder containing 65% of protein, 5-35 parts of wheat bran containing 14% of protein, 10-15 parts of calcium hydrogen phosphate containing 16% of phosphorus and 21% of calcium, 5-8 parts of copper sulfate heptahydrate containing 25% of copper, 5-15 parts of magnesium sulfate heptahydrate containing 9.7% of magnesium, 3-12 parts of zinc sulfate heptahydrate containing 22% of zinc and 3-10 parts of manganese sulfate containing 31% of manganese. The animal feed additive has comprehensive nutrients. When farmers blend materials, they only need to add the additive, and total dosage of the feed additive reaches 10-30% of the dosage of a feed. Thus, feed burdening intensity of farmers is reduced, and nutrient supply for culture products is guaranteed.

The invention discloses a medicine composition for treating pancreatitis and an application thereof. The composition is prepared from, by weight, 16-23 parts of pheretima aspergillum, 18-25 parts of rhizoma anemarrhenae, 18-25 parts of holothuria leucospilota, 18-25 parts of cortex albiziae, 11-20 parts of snakegourd fruits, 11-20 parts of bay leaves, 4-8 parts of compounds of glauber-salt and liquorice, 22-30 parts of oysters and 9-18 parts of Chinese lives. The selected medicinal materials are proper in compatibility, the remarkable curative effect is achieved for treating pancreatitis, the treatment course is short, effect taking is quick, and no relapse is caused after pancreatitis is cured. The medicine composition is safe, free of toxic and side effects, easy to take and low in cost, and can achieve the purpose of treating both symptoms and root causes.

The invention belongs to the field of traditional Chinese medicinal preparation and provides a medicine for treating epilepsy. The medicine for treating the epilepsy comprises poria with hostwood, tabasheer, polygala tenuifolia, cinnabar, acorus tatarinowii, selfheal, curcuma aromatica, pheretima aspergillum, dragon bone, white paeony root and salviae miltiorrhizae. The medicine for treating the epilepsy has the beneficial effects of quick effect, low toxic or side effect and ideal epilepsy treatment effect.

The invention discloses a traditional Chinese medicine for treating rheumatism. The traditional Chinese medicine is prepared from, by weight, 15 grams of pheretima aspergillum, 20 grams of the whole herb of arenaria kansuensis, 20 grams of snow lotus herb, 10 grams of notopterygium root, 10 grams of the rhizome of chuanxiong, 15 grams of clematis root, 30 grams of ginseng, 5 grams of crocus sativus, 20 grams of herba lycopodii, 20 grams of caulis sinomenii, 15 grams of rhizoma smilacis glabrae, 10 grams of radix angelicae, 8 grams of radix paeoniae rubra, 12 grams of fructus gardeniae, 20 grams of licorice root, 10 grams of fructus lycii, 15 grams of perilla stem, 10 grams of radix paeoniae alba, 10 grams of rhizoma cynanchi stauntonii, 10 grams of cassia twig, 15 grams of rhizoma pinelliae, 20 grams of cortex magnoliae officinalis, 10 grams of poria cocos, 10 grams of herba periliae, 7 grams of blackberrykiky rhizome, 8 grams of herba rabdosiae, 10 grams of pearl powder, 10 grams of flos caryophylli, 20 grams of eggplant roots, 15 grams of carapax et plastrum testudinis and 20 grams of notoginseng radix et rhizoma powder. The traditional Chinese medicine for treating the rheumatism has the advantages that the traditional Chinese medicine is scientifically obtained by combining with a large amount of concrete practice on the basis of the theory of traditional Chinese medicine,the traditional Chinese medicine is safe and reliable, can shorten the treatment time, has a significant treatment effect on various symptoms of the rheumatism, and is short in treatment cycle, and alarge number of true cases show that after short-term treatment, a return visit is paid, the rheumatism is treated, and the body does not hurt any more, moves freely, and has no uncomfortableness.