21 results about "PHERETIMA ASPERGILLUM" patented technology

The invention relates to a pheretima bombycis farm, and belongs to the technical field of earthworm breeding. The problem that earthworm suffocation death is caused by cross contamination of pathogenic microorganisms and unsmooth drainage due to mixing of functional areas of a traditional farm is solved. The layout of the farm is specifically as follows: a raw material pretreatment and storage area provided with a fermentation tank is arranged in the upwind direction of a site entrance; a core breeding production area is arranged in the center of the field, the interior of the core breeding production area is divided into an earthworm breeding area, a breeding area and a fattening area according to breeding stages, and mechanical channels are arranged in the areas and are of a ridge type structure; arranging an annular drainage ditch along the boundary of the field, and communicating the furrow with the annular drainage ditch by gradient; a finished product treatment and storage area containing an airing field is arranged in the downwind direction of the field exit; an isolation belt is arranged on the periphery, a management and auxiliary area containing a tool room is arranged at an entrance, and a feces treatment area containing a sedimentation tank is arranged in the downwind direction; the hardened main road is connected with all the functional areas, and the branch is communicated with the breeding district. According to the scheme, pollution source isolation and efficient drainage are achieved, and the method is mainly used for improving the earthworm survival rate and breeding benefits.

This invention relates to the field of traditional Chinese medicine technology, and more particularly to the application of a traditional Chinese medicine composition in the preparation of drugs for the prevention and treatment of lung injury. The traditional Chinese medicine composition includes Astragalus membranaceus, Eupolyphaga sinensis, Morus alba root bark, Fritillaria cirrhosa, Perilla frutescens seed, Lepidium apetalum seed, Prunus armeniaca seed, Pheretima aspergillum, Ephedra sinica, and Clematis chinensis. Experiments show that this traditional Chinese medicine composition can significantly reduce the level of the inflammatory factor TNF-α, decrease the lung index, and alleviate pathological damage to lung tissue, and can be used for the prevention and treatment of lung injury.

A blood-clearing and lipid-lowering pill is composed of the following ingredients in the indicated weight ratios: Astragalus membranaceus 54-74, Salvia miltiorrhiza 54-74, Paeonia lactiflora 54-74, Carthamus tinctorius 33-53, Pheretima aspergillum 54-74, Hirudo medicinalis 33-53, Crataegus pinnatifida 54-74, Polygonum multiflorum 54-74, Cassia tora 54-74, Gastrodia elata 33-53, Uncaria rhynchophylla 54-74, Eucommia ulmoides 33-53, Achyranthes bidentata 54-74, Os draconis 54-74, Ostrea gigas 54-74, Prunella vulgaris 54-74, and Alisma plantago-aquatica 54-74. This invention can reduce blood viscosity to normal levels in a short period, allowing some patients with vascular stenosis exceeding 75% who require stents to avoid stent grafting, and enabling stroke patients to gradually recover their function.

This invention proposes a traditional Chinese medicine compound for treating ankylosing spondylitis of the kidney-deficiency and damp-heat type accompanied by gastrointestinal dysfunction, along with its preparation method, belonging to the field of traditional Chinese medicine technology. The compound consists of Smilax glabra, Bletilla striata, Paris polyphylla, Geranium wilfordii, Dioscorea hypoglauca, Patrinia scabiosaefolia, Dipsacus asper, Drynaria fortunei, Pyrola rotundifolia, Clematis chinensis, Pheretima aspergillum, Lycopodium clavatum, Corydalis yanhusuo, Paeonia lactiflora, Atractylodes macrocephala (fried), Coix lacryma-jobi (raw), Aucklandia lappa, and Glycyrrhiza uralensis. This invention combines these herbs to achieve a synergistic effect, collectively clearing heat and dampness, strengthening the spleen and kidneys, and unblocking the meridians. The overall formula addresses both strengthening the body's foundation and eliminating pathogenic factors, ensuring unobstructed meridian flow and relieving arthralgia. This compound has the functions of clearing heat and dampness, strengthening the spleen and kidneys, and unblocking the meridians. It offers a short treatment course, rapid efficacy, low recurrence rate after cure, few side effects, and is inexpensive and convenient to use for treating ankylosing spondylitis with gastrointestinal dysfunction.

The invention provides a traditional Chinese medicine composition for treating chronic obstructive pulmonary disease. The traditional Chinese medicine composition is characterized by being prepared from the following components in parts by weight: 8-14 parts of radix astragali preparata, 3-7 parts of herba cistanche, 3-7 parts of fructus alpiniae oxyphyllae, 3-7 parts of rhizoma polygonati, 3-7 parts of radix dipsaci, 4-8 parts of rhizoma atractylodis macrocephalae fried with bran, 4-8 parts of radix salviae miltiorrhizae, 1-5 parts of semen brassicae, 2-6 parts of fructus perillae, 1-5 parts of semen lepidii, 1-5 parts of fructus trichosanthis, 1-5 parts of scorpio, 3-7 parts of earthworm, 1-5 parts of periostracum cicada, 1-5 parts of herba asari, 2-6 parts of radix saposhnikoviae and 2-6 parts of schizonepeta spike. The traditional Chinese medicine composition has the effects of regulating and tonifying lung and kidney, relieving cough and asthma, reducing phlegm and removing blood stasis, and is suitable for chronic obstructive pulmonary disease, chronic pulmonary heart disease, wheeze, chest distress and shortness of breath, aggravated motility, spontaneous perspiration and aversion to wind, cough, expectoration and other symptoms.

The present application belongs to the technical field of biological medicine, and particularly relates to an earthworm anti-platelet oligopeptide and application thereof. The present application is an anti-platelet oligopeptide discovered by combining a Pheretima aspergillum (E. Perrier) transcriptome database with active-oriented separation. The oligopeptide can not only inhibit the activation and aggregation of platelets, but also effectively inhibit the adhesion and expansion of platelets, and can effectively inhibit various functions of platelets at an in-vitro level. In summary, the oligopeptide of the present application can significantly inhibit platelets, and is suitable for anti-thrombus drug research and development.

This invention relates to the field of traditional Chinese medicine (TCM) technology, and particularly to a TCM composition, preparation, preparation method, and application for use in the peri-discharge period of acute exacerbations of COPD. The TCM composition comprises the following raw materials: 10-20 parts of *Codonopsis pilosula*, 10-25 parts of *Adenophora stricta*, 15-30 parts of *Clam shell*, 8-15 parts of *Fritillaria thunbergii*, 8-12 parts of *Platycodon grandiflorus*, 8-15 parts of *Paeonia lactiflora*, 8-12 parts of *Aster tataricus*, 8-12 parts of *Lepidium apetalum*, and 8-12 parts of *Pheretima aspergillum*. The TCM preparation derived therefrom can be in the following dosage forms: decoction, paste, tablets, capsules, granules, pills, powders, drop pills, mixtures, or oral liquids. It can be used to prepare drugs that relieve cough, sputum, and shortness of breath, and is suitable for the peri-discharge period of acute exacerbations of COPD.

This invention relates to the application of a traditional Chinese medicine composition in a drug for ulcerative colitis, characterized in that the traditional Chinese medicine composition comprises: 1-100 parts of Magnolia officinalis, 1-100 parts of Areca catechu (roasted), 1-100 parts of Amomum villosum (roasted), 1-100 parts of Ephedra sinica, 1-100 parts of Prunus armeniaca (bitter), 1-100 parts of Notopterygium incisum, 1-100 parts of Zingiber officinale (fresh), 1-100 parts of Pogostemon cablin, 1-100 parts of Eupatorium fortunei, 1-100 parts of Atractylodes lancea, 1-160 parts of Poria cocos, 1-120 parts of Atractylodes macrocephala, 1-100 parts of Gypsum fibrosum, 1-100 parts of Crataegus pinnatifida (roasted), 1-100 parts of Massa fermentata (roasted), 1-100 parts of Hordeum vulgare (roasted), 1-100 parts of Pheretima aspergillum, 1-100 parts of Cynanchum paniculatum, 1-100 parts of Dryopteris crassirhizoma, and 1-100 parts of Lepidium apetalum.

The invention relates to a medicine for treating cerebral thrombosis, cerebral thrombosis precursor and cerebral thrombosis sequelae. Comprising the following raw materials in parts by weight: 15-30 parts of astragalus membranaceus, 10-20 parts of ligusticum wallichii, 10-20 parts of radix paeoniae rubra, 10-20 parts of gastrodia elata, 2-5 parts of scorpion, 10-20 parts of pseudo-ginseng, 10-20 parts of rhizoma corydalis, 10-20 parts of radix clematidis, 10-20 parts of earthworm, 10-20 parts of honeysuckle stem, 10-20 parts of ginkgo leaf, 1-3 parts of centipede, 10-20 parts of cassia twig, 2-5 parts of leech and 10-20 parts of herba siegesbeckiae. The traditional Chinese medicine is orally taken and externally applied, and has the effects of dispersing blood stasis, relieving spasm, activating blood, relieving pain, tonifying qi and nourishing blood.

According to the formula, blood circulation promoting and stasis removing medicinal materials such as pseudo-ginseng, the root of red-rooted salvia, raw hawthorn peel and the like are used for improving brain blood circulation, eliminating meridian blockage and creating good conditions for nerve cell repair; the functions of viscera are regulated by virtue of spleen-invigorating and qi-replenishing medicinal materials such as codonopsis pilosula, roasted rhizoma atractylodis macrocephalae and fresh Chinese yam and yin-enriching and kidney-tonifying medicinal materials such as radix rehmanniae preparata and fructus lycii, and the healthy qi of the body is enhanced; multi-dimensional improvement is realized through combination of medicinal materials with different effects, such as radix scrophulariae and dried radish slices, herba asari and lumbricus for dispelling wind and dredging collaterals, rhizoma anemones altaicae and polygala tenuifolia for inducing resuscitation and inducing resuscitation; the composition pays attention to overall and local combination, has the basic functions of digestion-promoting spleen and stomach regulation of endothelium corneum gigeriae galli and malt, kidney-tonifying and nerve-calming liver and kidney nourishing of schisandra chinensis and pulp of dogwood fruit and the like while improving brain symptoms, and lays a foundation for long-term rehabilitation of wakened vegetative people; the raw materials are wide in source and relatively simple and convenient to prepare, most of the medicinal materials in the formula are common traditional Chinese medicinal materials and are easy to obtain, no special processing technology is adopted, and clinical application and popularization are facilitated.

This invention belongs to the field of pharmaceutical technology and relates to drug identification and detection methods, specifically to probe primers and their applications in the identification of *Pheretima aspergillum* and PCR detection methods. The probe primers consist of the sequences shown in SEQ ID NO. 1-8 and SEQ ID NO. 9-17; these probe primers are used for the specific and quantitative detection of *Pheretima aspergillum* using quantitative real-time PCR methods (including real-time quantitative PCR and digital PCR). The detection method provided by this invention can effectively detect *Pheretima aspergillum*, including newly discovered subgroups, exhibiting strong specificity and high sensitivity. It can be used for the qualitative or quantitative detection of *Pheretima aspergillum* in various forms of traditional Chinese medicine, including raw materials, processed slices, granules, decoctions, and compound preparations, demonstrating strong innovation and good application value.

This invention belongs to the field of molecular biology and relates to animal genetics and breeding. It specifically provides genes, primers, applications, and methods for identifying the developmental stages of the clitellum in *Pheretima aspergillum*. Addressing the shortcomings of existing artificial breeding methods for *Pheretima aspergillum*, which rely heavily on visual observation of the clitellum and suffer from significant physiological lag, this invention is the first to discover and screen specific molecular marker genes closely related to the initiation and maturity stages of clitellum differentiation in *Pheretima aspergillum*, and provides highly specific amplification primer sets and detection methods. This invention achieves very early molecular early warning and objective quantitative identification of clitellum development in *Pheretima aspergillum*, providing molecular standards for the quantitative control of nutritional intervention and mating / harvesting timing in artificial breeding. This technology helps to break through the bottleneck of large-scale *Pheretima aspergillum* farming, reduce dependence on wild resources, and is of great significance for protecting ecological red lines and the safety of the traditional Chinese medicine industry.

This invention discloses a combined acupuncture and medication method for treating cervicogenic vertigo, belonging to the field of traditional Chinese medicine clinical treatment technology. The method comprises two parts: oral administration of a specific herbal composition and standardized acupuncture knife therapy. The herbal composition consists of Pinellia ternata, Gastrodia elata, Uncaria rhynchophylla, Poria cocos, Citrus reticulata peel, stir-fried Atractylodes macrocephala, Zingiber officinale, Haliotis diversicolor, Pueraria lobata, Cinnamomum cassia, Corydalis yanhusuo, Ziziphus jujuba, Lycopodium clavatum, Spatholobus suberectus, Pheretima aspergillum, Chaenomeles speciosa, and Paeonia lactiflora in specific dosages, which are then sterilized, preserved, dried, pulverized, and sieved to form capsules for oral administration. The acupuncture knife therapy targets Baihui (GV20), the midpoint of the neck, the left point of the neck, the right point of the neck, and the articular process points. This invention has the effects of resolving phlegm and relieving wind, strengthening the spleen and removing dampness, calming liver yang, relieving exterior cold, and relaxing tendons and vessels, far exceeding the effects of Western medicine treatment alone. It is most effective for the phlegm-dampness obstruction type. It has the advantages of being safe with no side effects, treating both the symptoms and the root cause, having a low recurrence rate, a high degree of standardization, and a wide range of applications. It improves the pathological state of cervicogenic vertigo from the root, embodying the concept of preventive medicine in traditional Chinese medicine, and has significant clinical application value.

The invention discloses a preparation method and application of an alpine horseradish water extract for relieving cough and resisting inflammation, and relates to the technical field of preparation of cough-relieving and anti-inflammatory medicines. The traditional Chinese medicine composition comprises the following components in parts by weight: 60-80 parts of alpine horseradish, 25-35 parts of perilla leaves, 18-28 parts of bulbus fritillariae cirrhosae, 12-22 parts of rhizoma anemarrhenae, 10-20 parts of white mulberry root-bark, 8-15 parts of lumbricus, 6-12 parts of cynanchum glaucescens and 5-10 parts of blackberry lily. According to the alpine horseradish water extract disclosed by the invention, alpine horseradish is taken as a monarch drug, and rich flavonoid components in the alpine horseradish can effectively inhibit release of inflammatory factors and relieve cough reflex; ministerial drugs such as perilla leaves and bulbus fritillariae cirrhosae are supplemented, volatile oil components of the perilla leaves can enhance the relaxation effect of airway smooth muscles, and alkaloid of the bulbus fritillariae cirrhosae can cooperatively relieve a cough and dilute sputum; the components such as rhizoma anemarrhenae and the root bark of white mulberry are used as assistants, saponin substances of the rhizoma anemarrhenae can enhance the anti-inflammatory activity, and flavonoid components of the root bark of white mulberry can relieve airway mucosa edema; the earthworm, the cynanchum glaucescens and the blackberry lily are used, plasmin of the earthworm can improve airway microcirculation, and saponin of the cynanchum glaucescens can enhance the cough relieving effect.

This invention relates to the field of traditional Chinese medicine (TCM) technology, and particularly to the application of TCM compositions in the preparation of drugs for the prevention and treatment of asthma. The TCM composition includes Astragalus membranaceus, Eupolyphaga sinensis, Morus alba root bark, Fritillaria cirrhosa, Perilla frutescens seed, Lepidium apetalum seed, Prunus armeniaca seed, Pheretima aspergillum, Ephedra sinica, and Clematis chinensis. Experiments show that the TCM composition provided by this invention can effectively improve airway hyperresponsiveness, reduce lung resistance, and enhance dynamic lung compliance in asthma model animals. Simultaneously, it can significantly reduce the content of pro-inflammatory factors IL-4 and IL-13 in the bronchoalveolar lavage fluid of asthma models, alleviate airway inflammation, and relieve asthma pathological symptoms. It can stably exert its effect in preventing and treating asthma, and its efficacy is stable and its safety is good. It can be further developed into a drug for the prevention and treatment of asthma and has good clinical application prospects.

The invention relates to the field of traditional Chinese medicine preparations, in particular to a medicine for promoting perianal healing and a preparation method thereof. Comprising the following components in parts by weight: 5-15 parts of radix arnebiae seu lithospermi, 1-5 parts of rhizoma bletillae, 5-10 parts of radix angelicae sinensis, 5-10 parts of radix rehmanniae, 5-10 parts of cortex phellodendri, 1-5 parts of radix et rhizoma rhei, 15-20 parts of radix astragali, 5-10 parts of lumbricus and 5-10 parts of radix angelicae, and the components are prepared into 80-200-mesh medicine powder. The preparation method comprises the following steps: mixing the decocted first concentrated solution, second concentrated solution and fine powder, grinding, and sieving. The effects of cooling blood, detoxifying, promoting tissue regeneration and astringing are achieved. The ointment has the effects of inhibiting inflammation, accelerating formation of granulation tissues of crissum wound surfaces, reducing dressing change times, promoting wound surface healing, relieving scar formation and preventing relapse. The device is suitable for wound healing and rehabilitation in a perianal high-humidity high-friction-force complex pathological microenvironment in which multiple microorganisms are colonized.

This invention belongs to the field of traditional Chinese medicine and relates to a traditional Chinese medicine composition for treating early diabetic nephropathy, its preparation method, and its application. The composition consists of: 15-45g of raw Astragalus membranaceus, 10-15g of Pseudostellaria heterophylla, 9-15g of Salvia miltiorrhiza, 9-15g of Paeonia lactiflora, 10-15g of Achyranthes bidentata, 3-6g of Pheretima aspergillum, 10-15g of Centella asiatica, 9-12g of Hedyotis diffusa, 15-30g of Euryale ferox, 9-12g of Broussonetia papyrifera, 9-12g of Mahonia japonica, 9-12g of Euonymus alatus, 10-15g of Poria cocos, 9-12g of Citrus reticulata peel, and 15-30g of Zea mays silk (wrapped and decocted). The beneficial effects of this invention are: strengthening the spleen and kidneys, replenishing qi and nourishing yin, promoting blood circulation and unblocking collaterals, and resolving blood stasis and eliminating turbidity. This medicine is used for early diabetic nephropathy caused by deficiency of both qi and yin, insufficiency of spleen and kidney, and internal obstruction of blood stasis and turbid toxins. The following symptoms may be observed: cloudy or foamy urine, soreness and weakness of the lower back and knees, fatigue and weakness, dry mouth and throat, mild edema of the limbs (especially the lower limbs), pale complexion, or numbness of the limbs, pale or dark red tongue, or with petechiae and ecchymosis, thin white or slightly greasy tongue coating, and thready or deep and thready pulse.

The invention belongs to the technical field of traditional Chinese medicines and provides a traditional Chinese medicine composition for treating acute and chronic cough and a preparation method thereof. The composition comprises thunberg fritillary bulb, trichosanthes kirilowii maxim, white mulberry root-bark, honeysuckle, fructus forsythiae, scutellaria baicalensis, radix isatidis, rhizoma pinelliae preparata, semen armeniacae amarae, fructus arctii, poria cocos, pericarpium citri reticulatae, lumbricus, peach kernel, platycodon grandiflorum, magnolia flower, lily and liquorice, is mainly used for treating the phlegm-heat obstructing lung syndrome, and has the common symptoms of cough, yellow and thick phlegm cough, fever, asthma, red, swollen and painful throat or nasal obstruction, dry mouth and the like. The traditional Chinese medicine composition disclosed by the invention has a very good curative effect on acute bronchitis, acute attack of chronic bronchitis, cough allergic asthma and cough after cold, particularly can quickly relieve the symptoms such as catarrhal symptom, nasal obstruction, saliva, excessive sputum and cough, and has a wide market application prospect.

The invention belongs to the technical field of biological medicine, and particularly relates to earthworm anticoagulant oligopeptide and application thereof. The invention relates to an anticoagulant oligopeptide, which is found by combining the activity-directed separation of pheretima asper (E. Perriver) with the screening of an AI (artificial intelligence) prediction model. The amino acid sequence of the oligopeptide is LMWFSDRNK; the oligopeptide regulates thrombin in an allosteric manner, enhances the combination of thrombin and antithrombin, and can effectively inhibit blood coagulation and thrombus formation at in-vivo and in-vitro levels; the oligopeptide has a remarkable anticoagulant effect and is suitable for research and development of antithrombotic drugs.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition and a preparation and application thereof. The traditional Chinese medicine composition is composed of a first component, a second component and a third component according to the mass ratio of 1: (1-2): (0.2-0.8), wherein the first component contains oldenlandia diffusa, sculellaria barbata and gynostemma pentaphylla, is a monarch drug and directly attacks damp toxin and blood stasis; the second component contains radix astragali, rhizoma atractylodis macrocephalae, semen coicis, polyporus umbellatus, poria cocos and taxus chinensis, is a ministerial drug and an adjuvant drug, and is used for invigorating spleen to eliminate dampness and inducing diuresis to And the third component comprises curcuma zedoary, pseudo-ginseng and lumbricus which are used as conductant drugs and can promote blood circulation to remove meridian obstruction and harmonize the drugs. Experiments and clinical verifications show that the traditional Chinese medicine composition provided by the invention can significantly improve proteinuria, regulate lipid metabolism, protect kidney structure and delay fibrosis, is suitable for patients with different conditions, is scientific in compatibility and convenient to prepare, and can be used for preparing medicines for treating membranous nephropathy with syndrome of damp toxin and blood stasis.

The invention discloses a composition for treating post-infection cough as well as a preparation, a preparation method and application thereof, and relates to the technical field of traditional Chinese medicines. The composition is prepared from the following components in parts by weight: 8 to 12 parts of rhizoma pinellinae praeparata, 8 to 12 parts of radix scutellariae, 4 to 8 parts of rhizoma coptidis, 8 to 13 parts of rhizoma zingiberis, 2 to 5 parts of herba asari, 8 to 10 parts of liquorice root, 2 to 6 parts of fructus ziziphi jujubae, 8 to 10 parts of fructus perillae, 9 to 12 parts of semen armeniacae amarae, 18 to 24 parts of rhizoma dioscoreae nipponicae, 18 to 25 parts of earthworm, 8 to 15 parts of radix asteris, 9 to 12 parts of flos farfarae, 8 to 10 parts of cortex magnoliae officinalis, 18 to 20 parts of poria cocos, 30 to 40 parts of radix astragali seu hedysari, 12 to 16 parts of radix adenophorae and 8 to 10 parts of fructus According to specific syndromes of patients (such as pharyngalgia with trollflower, dry throat with radix ophiopogonis, constipation with trichosanthes kirilowii maxim, pharynx itching with cicada slough, and excessive phlegm with thunberg fritillary bulb), the traditional Chinese medicine composition can fundamentally promote recovery and reduce relapse, can realize individual treatment of one person with one prescription, and greatly improves clinical applicability and curative effect precision.