Micro-fluidic chemiluminescence detecting system for magnetic particles for detecting thyroid functions

Abstract

The invention discloses a micro-fluidic chemiluminescence detecting system for magnetic particles for detecting thyroid functions. The system comprises a bottom plate and an upper chip, and the upperchip comprises a sample adding area in the center of the upper chip and five micro-fluidic reaction detecting channels communicated with the sample adding area, wherein the five micro-fluidic reactiondetecting channels include a total triiodothyronine micro-fluidic reaction detecting channel, a total thyroxine micro-fluidic reaction detecting channel, a free triiodothyronine micro-fluidic reaction detecting channel, a free thyroxine micro-fluidic reaction detecting channel and a thyroid stimulating hormone micro-fluidic reaction detecting channel. In application of the system, the bottom plate is arranged under the upper chip, and magnets which are permanent magnets or electromagnets are arranged at positions, corresponding to magnetic particle coating areas of the micro-fluidic reactiondetecting channels, of the bottom plate. By ingenious combination of the micro-fluidic technology and the magnetic particle chemiluminescence technology, quickness, high sensitivity and accuracy in quantitative detection of target substances are realized.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a microfluidic chemiluminescent detection system of magnetic particles used for detecting thyroid function. Background technique [0002] In recent years, the field of bioanalysis technology has developed rapidly, and many important research directions have emerged. Microfluidic chip analysis technology is one of the most active ones, and has received extensive attention in both scientific research and application fields. As a new type of analysis and detection platform, microfluidic chips have the advantages of high throughput, integration, portability, easy operation, and low cost, and have been widely used in many fields. [0003] The current chemiluminescence detection is suitable for serum, and the sample needs to be centrifuged to obtain serum during the detection process, which is complex, tedious and time-consuming. Lack of sufficient elution to eliminate interference cau...

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Problems solved by technology

[0005] Total triiodothyronine (TT 3 ), total thyroxine (TT 4 ), free triiodothyronine (FT 3 ), free thyroxine (FT 4 ), Thyroid-stimulating hormone (TSH) belong to the gonad detection series, patients often need to detect a series of items in thyroid function, but in reality, all of them are individually packaged for a single test item, according to the current method, laboratory doctors need to test item by item Performing laboratory tests requires heavy workload, repetitive and cumbersome testing process, and takes up a lot of human and material resources

In addition, the substrate reagents and cleaning solutions used in the same series of testing items are the same. If the series of kits are repeatedly equipped with the same substrate reagents and cleaning solutions, it will cause waste of reagents and reagent bottles. Invisibly increased production costs

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