24-site micro-array screen printing electrochemical sensing device and application thereof

A technology of screen printing and sensing device, applied in electrochemical variables of materials, measuring devices, scientific instruments, etc., can solve the problems of pollution, cross-channel, low sensor sensitivity, etc., achieve accurate quantitative detection, and overcome long detection time. Effect

Pending Publication Date: 2018-03-06
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no electrochemical immunosensor for the simultaneous detection of enrofloxacin and melamine, and the existing sensors used for actual detection are not very sensitive. During the electrode preparation and detection process in channel electrochemical detection, it is easy to produce Pollution and cross-talk

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • 24-site micro-array screen printing electrochemical sensing device and application thereof
  • 24-site micro-array screen printing electrochemical sensing device and application thereof
  • 24-site micro-array screen printing electrochemical sensing device and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Design and fabrication of 24-site microarray screen-printed electrochemical sensing device

[0033] Such as figure 1 As shown, a 24-site microarray screen-printed electrochemical sensing device is composed of four parts: a sliding layer 1, an insulating layer 2, a base layer 3 and a support layer 4, and the base layer and the sliding layer form a double-layer sliding structure. The conductive printed circuit is collected at the printed circuit interface 8 at the end of the base layer and is used to connect the electrochemical workstation after being combined with the connector. The supporting layer 4 is used to fix the whole device and control the sliding range. The insulating layer 2 is used to define the reaction area.

[0034]Its base layer 3 is printed with 24 working electrodes and integrated circuits, and circular hollow structures 12 and rectangular hollow structures 11 are arranged crosswise on the sliding layer. By sliding up and down, the sliding layer a...

Embodiment 2

[0048] Determination of Enrofloxacin and Melamine Content in Actual Samples:

[0049] Using the 24-site microarray screen printing electrochemical sensing device of the present invention and the working electrode modified by graphene materials, the Enroza in actual samples (whole milk, skimmed milk powder, infant milk powder) was treated by immunosuppressive method The star and melamine were analyzed and determined, and the standard addition method was used to carry out the standard addition recovery experiment. The differential pulse method in the CHI electrochemical workstation system was selected for detection, and the scanning range of the differential pulse method was -0.3-0.5v. Measure in parallel three times, read the current response value and calculate the average value, bring it into the standard curve formula, and calculate the corresponding concentration. The recoveries obtained are 90.18%-100.76% and 91.34%-108.81%, respectively, indicating that the use of the 24...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a 24-site microarray screen printing electrochemical sensing device, which comprises a sliding layer arranged in sequence from top to bottom, an insulating layer used to limit the reaction area, a base layer and a support layer used to fix the whole device , the base layer and the insulating layer are fixed on the support layer, the sliding layer and the support layer are slidingly connected, and the conductive printed circuit is collected at the end of the base layer and combined with the joint to connect the electrochemical workstation; and discloses application of this device. The advantage of the present invention is that it combines the traditional immune technology with the sensor technology, and provides a new fast, convenient and sensitive detection method for the trace detection of small molecular substances enrofloxacin and melamine, and can simultaneously realize multi-channel, Detection of multiple samples. It overcomes the technical problems of the original detection method, such as only targeting a single detection object, long detection time, expensive instruments, complicated operation, low sensitivity, poor specificity, and small detection range.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a 24-site microarray screen-printed electrochemical sensing device which is especially used for the detection of enrofloxacin and melamine. Background technique [0002] Enrofloxacin is a broad-spectrum antibacterial drug, which is often used in the treatment of bacterial infectious diseases. The residue of enrofloxacin in animal products such as dairy products will lead to human ingestion. Excessive intake can cause nerve damage, digestive system damage and allergic reactions. [0003] Melamine itself has no nutrition and is an illegal additive, which is usually added to dairy products by unscrupulous traders to create the illusion of high protein. Excessive intake may lead to human urinary tract cancer, bladder cancer and kidney failure. [0004] The electrochemical immunosensor combines electrochemical detection technology and immunoassay technology t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/48G01N27/3277G01N27/3278
Inventor 王俊平顾颖王硕潘明飞刘冰
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap