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24-site micro-array screen printing electrochemical sensing device and application thereof

A technology of screen printing and sensing device, applied in electrochemical variables of materials, measuring devices, scientific instruments, etc., can solve the problems of pollution, cross-channel, low sensor sensitivity, etc., achieve accurate quantitative detection, and overcome long detection time. Effect

Pending Publication Date: 2018-03-06
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there is no electrochemical immunosensor for the simultaneous detection of enrofloxacin and melamine, and the existing sensors used for actual detection are not very sensitive. During the electrode preparation and detection process in channel electrochemical detection, it is easy to produce Pollution and cross-talk

Method used

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  • 24-site micro-array screen printing electrochemical sensing device and application thereof
  • 24-site micro-array screen printing electrochemical sensing device and application thereof
  • 24-site micro-array screen printing electrochemical sensing device and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] (1) Design and fabrication of 24-site microarray screen-printed electrochemical sensing device

[0033] Such as figure 1 As shown, a 24-site microarray screen-printed electrochemical sensing device is composed of four parts: a sliding layer 1, an insulating layer 2, a base layer 3 and a support layer 4, and the base layer and the sliding layer form a double-layer sliding structure. The conductive printed circuit is collected at the printed circuit interface 8 at the end of the base layer and is used to connect the electrochemical workstation after being combined with the connector. The supporting layer 4 is used to fix the whole device and control the sliding range. The insulating layer 2 is used to define the reaction area.

[0034]Its base layer 3 is printed with 24 working electrodes and integrated circuits, and circular hollow structures 12 and rectangular hollow structures 11 are arranged crosswise on the sliding layer. By sliding up and down, the sliding layer a...

Embodiment 2

[0048] Determination of Enrofloxacin and Melamine Content in Actual Samples:

[0049] Using the 24-site microarray screen printing electrochemical sensing device of the present invention and the working electrode modified by graphene materials, the Enroza in actual samples (whole milk, skimmed milk powder, infant milk powder) was treated by immunosuppressive method The star and melamine were analyzed and determined, and the standard addition method was used to carry out the standard addition recovery experiment. The differential pulse method in the CHI electrochemical workstation system was selected for detection, and the scanning range of the differential pulse method was -0.3-0.5v. Measure in parallel three times, read the current response value and calculate the average value, bring it into the standard curve formula, and calculate the corresponding concentration. The recoveries obtained are 90.18%-100.76% and 91.34%-108.81%, respectively, indicating that the use of the 24...

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Abstract

The invention discloses a 24-site micro-array screen printing electrochemical sensing device. The 24-site micro-array screen printing electrochemical sensing device comprises a sliding layer, an insulating layer for limiting a reaction area, a basic layer and a supporting layer for fixing the whole device which are sequentially arranged from top to bottom, wherein the basic layer and the insulating layer are fixed to the supporting layer; the sliding layer is in sliding connection with the supporting layer; a conductive printed circuit board is converged to the tail end of the basic layer andis connected with a joint, and is used for connecting an electrochemical working station. The invention also discloses application of the device. The 24-site micro-array screen printing electrochemical sensing device and the application of the device have the advantages that through combination of a conventional immunization technique and a sensor technique, a new quick, convenient and sensitive detection method is provided for trace detection of enrofloxacin and tripolycyanamide small-molecule substances; meanwhile, multi-channel and detection of multiple samples can be realized; moreover, the technical problems that the detection time is long, an instrument is expensive, an operation is complicated, the sensitivity is low, the speciality is poor, the detection range is narrow and the like when an original detection method only aims to a single detection material are solved.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a 24-site microarray screen-printed electrochemical sensing device which is especially used for the detection of enrofloxacin and melamine. Background technique [0002] Enrofloxacin is a broad-spectrum antibacterial drug, which is often used in the treatment of bacterial infectious diseases. The residue of enrofloxacin in animal products such as dairy products will lead to human ingestion. Excessive intake can cause nerve damage, digestive system damage and allergic reactions. [0003] Melamine itself has no nutrition and is an illegal additive, which is usually added to dairy products by unscrupulous traders to create the illusion of high protein. Excessive intake may lead to human urinary tract cancer, bladder cancer and kidney failure. [0004] The electrochemical immunosensor combines electrochemical detection technology and immunoassay technology t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 王俊平顾颖王硕潘明飞刘冰
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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