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Thyroid organoid culture medium and thyroid organoid culture and passage method

A technology of thyroid and organoids, applied in the field of biomedicine, can solve the problems of inability to effectively culture thyroid organoids, achieve good thyroid organoid culture, promote the formation of thyroid organoids, and improve the effect of cloning formation rate

Pending Publication Date: 2022-04-12
川北医学院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For this reason, the present invention provides a kind of thyroid organoid culture medium, thyroid organoid culture and passaging method, to solve the problem that prior art can't effectively cultivate thyroid organoid

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  • Thyroid organoid culture medium and thyroid organoid culture and passage method
  • Thyroid organoid culture medium and thyroid organoid culture and passage method
  • Thyroid organoid culture medium and thyroid organoid culture and passage method

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Embodiment 1

[0054] This example provides a thyroid organoid culture medium, including Advanced DMEM / F12 medium and the following components: B-27, N-acetylcysteine, nicotinamide, R-spondin1 recombinant protein, Noggin, Wnt- 3a, epidermal growth factor, A83-01, thyrotropin, hydrocortisone, insulin, fibroblast growth factor-10, Y27632, FGF7, and SB202190.

[0055] In the thyroid organoid culture medium of this embodiment, the concentration of B-27 is A (1x), the concentration of N-acetylcysteine ​​is 2.5mM, and the concentration of nicotinamide is 10mM, so The concentration of the R-spondin1 recombinant protein is 200ng / mL, the concentration of the Noggin is 100ng / mL, the concentration of the Wnt-3a is 30ng / mL, and the concentration of the epidermal growth factor is 50ng / mL, so The concentration of the A83-01 is 1mM, the concentration of the thyrotropin is 15mIU / mL, the concentration of the fibroblast growth factor-10 is 100ng / mL, the concentration of the Y27632 is 10μM, and the hydrocortis...

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Abstract

The invention discloses a thyroid organoid culture medium and a thyroid organoid culture and passage method. The thyroid organ culture medium comprises an Advanced DMEM (Dulbecco's Modified Eagle Medium) / F12 culture medium and the following components: B-27, N-acetylcysteine, nicotinamide, R-spondin1 recombinant protein, head protein, Wnt-3a, an epidermal growth factor, A83-01, a thyroid stimulating hormone, hydrocortisone, insulin, a fibroblast growth factor-10 and Y27632. The thyroid stimulating hormone, the hydrocortisone and the insulin are innovatively added into the thyroid organ culture medium, the thyroid organ formation can be remarkably promoted by adding the thyroid stimulating hormone, the cell proliferation and growth effect can be effectively promoted by adding the hydrocortisone, the clone formation rate can be increased, and the culture medium has the advantages that the cost is reduced; by adding insulin, the dryness of cells can be maintained, the passage efficiency is improved, and the thyroid organ culture medium can be used for culturing thyroid organs very well.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a culture medium for thyroid organoids, a method for culturing and passage of thyroid organoids. Background technique [0002] The thyroid gland is a very important gland in vertebrates and belongs to the endocrine organ. Located below the thyroid cartilage in the neck of mammals, on both sides of the trachea. Thyroid cancer is the most common thyroid malignancy, accounting for about 1% of systemic malignancies, including four pathological types: papillary carcinoma, follicular carcinoma, undifferentiated carcinoma, and medullary carcinoma. Papillary carcinoma with low malignancy and good prognosis is the most common. Except for medullary carcinoma, most thyroid carcinomas originate from follicular epithelial cells. The treatment of thyroid cancer mainly depends on the patient's age, pathological type of tumor, degree of lesion and general condition, etc., with surgery as t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 胥正敏周春阳温虎玲何冠军王玉泉陈健赵永生雍玺尤俊卞娇娇黄蓉夏天琴郭文林鑫静
Owner 川北医学院
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