Ovulation detection test strip and detection method

A technology for detecting test strips and detection lines, which is applied in the field of ovulation detection test strips and detection, which can solve the problems of abnormal interpretation of results, poor stability, high cost, etc., and achieve the effects of accurate detection results, guaranteed accuracy, and good specificity
CN112730854APending Publication Date: 2021-04-30杭州新脉生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
杭州新脉生物科技有限公司
Publication Date
2021-04-30

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Abstract

The invention discloses an ovulation detection test strip. The ovulation detection test strip comprises a PVC bottom plate, a sample pad, a gold label pad, a nitrocellulose film and a water absorption pad, wherein the nitrocellulose film is provided with a detection line T1, a detection line T2 and a quality control line, the detection line T1 is coated with a mouse anti-human beta LH monoclonal antibody 1A4, the detection line T2 is coated with a cotinine antigen, and the quality control line is coated with a goat anti-chicken IgY polyclonal antibody; and a colloidal gold mouse anti-human beta LH monoclonal antibody 6B8 conjugate, a colloidal gold chicken IgY antibody conjugate and a colloidal gold cotinine monoclonal antibody conjugate are cured on the gold label pad. The test strip is high in detection sensitivity and good in specificity, abnormal interpretation results caused by crossing of LH with follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH) can be avoided, detection results are more accurate, and monitoring of the smoking metabolite cotinine in urine is combined. The invention further discloses an ovulation detection method, the steps are simple, and the test result is accurate.
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Description

technical field

[0001] The invention relates to an ovulation detection test paper, in particular to an ovulation detection test paper strip and a detection method. Background technique

[0002] Luteinizing hormone (LH) is a substance secreted by basophils of the pituitary gland. In females (LH) works in concert with follicle-stimulating hormone (FSH) to maintain the ovarian menstrual cycle, leading to ovulation and corpus luteum formation. The production of LH is controlled by the hypothalamic gonadotropin-releasing hormone, and is also regulated by positive and negative feedback from the ovary. The release peak of LH in the menstrual cycle is closely related to ovarian ovulation. Once the LH peak appears, it indicates ovarian ovulation in 24-36 hours. Therefore, the peak serum LH can be monitored during the menstrual cycle to determine the best time for conception. The test results are expressed in milliinternational units / milliliter (mIu / ml).

[0003] LH is a glycoprote...

Claims

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