Thyroid function detection protein chip and kit thereof

A thyroid function and protein chip technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of single detection capability, no integration, high detection cost, reduce detection cost, save time and resources, improve The effect of detection efficiency

Inactive Publication Date: 2011-06-15
上海裕隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the shortcomings of single detection ability, high detection cost

Method used

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  • Thyroid function detection protein chip and kit thereof
  • Thyroid function detection protein chip and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Preparation of a thyroid function detection protein chip

[0022] The main steps are: (1) prepare the matrix nitrocellulose membrane; (2) design the chip, use FT3 antibody and FT4 antibody as the coated antibody in the reaction wells of odd rows, and use T3 antibody and T4 antibody in the reaction wells of even rows. Antibodies and TSH antibodies were used as coated antibodies; (3) The sampling needle sucked out the antibody from the 384-well plate and sprayed it on the nitrocellulose membrane; (4) Assemble the nitrocellulose membrane, and then sealed, dried, packaged, and stored.

[0023] The preparation method of the above-mentioned protein chip for detecting thyroid function specifically includes the following steps:

[0024] 1. Determination of antigens and antibodies related to thyroid function test:

[0025] The antigen used to prepare the thyroid function detection protein chip detection index was purchased from Fitzgerald;

[0026] FT3, FT4 analogs ...

Embodiment 2

[0032] Embodiment 2: the preparation of kit

[0033] A protein chip kit for thyroid function detection, which consists of a protein chip for thyroid function detection, standard products, quality control products, sample diluents, enzyme-labeled working solutions, concentrated washing solutions, and detection solutions; each part is bottled, with a paper template It is fixed in the packing box, and the placement method is not limited, and it is used together with the test. Here's how the parts are prepared:

[0034] 1. The thyroid function detection protein chip is a 6×8 well plate, with nitrocellulose membrane as the base, and the assembly method is shown in ZL032298900.

[0035] 2. The standard product provides 12 tubes, each tube is 0.2ml.

[0036] With 3% BSA and 0.05% preservatives (Proclin TM 300) of phosphate buffer (pH 7.4) configuration, wherein the final concentrations of T3, T4, FT3, FT4 and TSH standards are shown in the table below:

[0037]

[0038] 3. Pr...

Embodiment 3

[0052] Example 3: Application and detection of protein chips and kits

[0053] The application of the protein chip for detecting thyroid function of the present invention mainly uses the competition method and the double-antibody sandwich method to detect relevant indicators in human blood. The method is to add human serum to the surface of the chip, and then add the enzyme-labeled working solution to react directly; 1 minute after adding the detection solution, scan and collect the signal, and the signal intensity of the sample point is linearly related to the concentration of the antigen in the serum.

[0054] In the application of the above-mentioned protein chip kit for detecting thyroid function, the specific methods of antigen-antibody reaction and detection are as follows:

[0055] (1) Use 3% BSA solution to block the chip of sprayed antibody, positive control and negative control, and block the non-specific sites on the surface of the substrate for 2 hours at room temp...

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Abstract

The invention discloses a thyroid function detection protein chip and a kit thereof. The kit comprises the thyroid function detection protein chip, wherein the chip is coated with a free triiodothyronine (FT3) antibody, a free thyroxine (FT4) antibody, a triiodothyronine (T3) antibody, a thyroxine (T4) antibody and a thyroid stimulating hormone (TSH) antibody. The kit can quantitatively detect the content of FT3, FT4, T3, T4 and TSH in patient serum, and is used for the auxiliary diagnosis of thyroid dysfunction diseases. Compared with the conventional enzyme linked immunosorbent assay (ELISA) technology, the chemiluminescence immunoassay keeps high specificity of the ELISA technology, stability and reliability of a detection result, and convenience of operation, and can improve detection sensitivity; and various indexes can be detected simultaneously, so time and cost are greatly saved.

Description

technical field [0001] The invention belongs to the technical field of in vitro clinical testing and chemiluminescence immunoassay, and in particular relates to a protein chip for thyroid function detection and a kit thereof. Background technique [0002] The thyroid gland is the largest endocrine gland in the human body and can secrete hormones such as thyroxine (T4) and triiodothyronine (T3). These hormones are regulated by thyrotropin (TSH) secreted by the pituitary gland, and play a very important role in the metabolism of protein, fat, sugar, vitamins, water and salt and the growth and development of the human body. Thyroid dysfunction is the most common endocrine disease, and it is still easily underdiagnosed. According to the census of 46,000 people in 11 cities in the United States, it is found that about 11% of the people have various thyroid dysfunctions that have not been diagnosed. Thyroid dysfunction can be divided into hyperthyroidism (referred to as hyperthy...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/544G01N21/76
Inventor 穆海东汪宁梅穆宇豪王通
Owner 上海裕隆生物科技有限公司
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