120 results about "Structural analog" patented technology

The invention discloses corynebacterium glutamicum for producing L-isoleucine and application of the corynebacterium glutamicum. The strain is obtained by performing ultraviolet mutagenesis and structural analogue resistance screening on corynebacterium glutamicum ATCC13869. According to the method, the L-isoleucine can be produced through direct fermentation of glucose, the yield of the L-isoleucine can reach 51.69 g / L, the conversion rate can reach 28.5%, the production efficiency is improved, meanwhile, the main raw material glucose used for fermentation is low in price and easy to obtain, the method conforms to the green food safety production concept, and industrial production of the L-isoleucine is facilitated.

The invention discloses a nucleic acid aptamer OUC-Fx11-5T of fucoxanthin and application of the nucleic acid aptamer OUC-Fx11-5T, and belongs to the technical field of nucleic acid aptamers. The nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine has a nucleotide sequence as shown in SEQ ID NO. 19. The nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine has a nucleotide sequence as shown in SEQ The invention further discloses the application of the nucleic acid aptamer OUC-Fx11-5T of the fucoxanthin in identification or detection of the fucoxanthin. According to the nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine, the affinity and dissociation constant of the nucleic acid aptamer OUC-Fx11-5T and the fucoxanthine is determined to be 68.2 nM through an isothermal titer thermal method, the nucleic acid aptamer OUC-Fx11-5T has no obvious affinity to structural analogues of the fucoxanthine such as violaxanthin, neoxanthin, zeaxanthin and beta-carotene, has high affinity and good specificity to the fucoxanthine, and can be used for preparing the fucoxanthine aptamer OUC-Fx11-5T of the fucoxanthine. The method can be used for identification or detection of fucoxanthine.

The invention discloses an anti-trifloxystrobin monoclonal antibody and an application thereof. A light chain variable region of the anti-trifloxystrobin monoclonal antibody has an amino acid sequence as shown in SEQ ID No.1, and a heavy chain variable region of the anti-trifloxystrobin monoclonal antibody has an amino acid sequence as shown in SEQ ID No.2. The monoclonal antibody has good affinity to trifloxystrobin, the half inhibitory concentration (IC50) of the monoclonal antibody is 4.55 ng / mL, the detection limit (IC10) of the monoclonal antibody is 1.34 ng / mL, the linear range (IC20-IC80) of the monoclonal antibody is 2.11-9.83 ng / mL, the cross reaction rate of the monoclonal antibody to structural analogues such as enestroburin, picoxystrobin and metoxystrobin is smaller than 1%, and the monoclonal antibody is high in specificity and has the advantages of being good in thermal stability, high in organic solvent and acid-base tolerance and high in sensitivity. The method is suitable for detecting trifloxystrobin residues in different environments.

The invention relates to a hybridoma cell strain capable of secreting a mefos monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. According to the invention, the hybridoma cell strain capable of secreting the fenofos monoclonal antibody is obtained through screening, the fenofos monoclonal antibody has high specificity and high sensitivity to the fenofos, IC50 to the fenofos is 0.231 ng / mL, and the fenofos monoclonal antibody has no cross reaction to structural analogues of the fenofos, such as triazophos, diazinon, phorate, acetyl phorate, methamidophos and the like; therefore, low-concentration isoprophos can be accurately detected.

The invention relates to a hybridoma cell strain capable of secreting dextromethorphan monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. The monoclonal antibody secreted by the hybridoma cell strain disclosed by the invention has good sensitivity and specificity on dextromethorphan, wherein the IC50 value on the dextromethorphan is 0.162 ng / mL. The monoclonal antibody provided by the invention has no cross reaction on structural analogues of dextromethorphan, such as fentanyl, thilazine, papaverine, morphine and medetomidine, and has good specificity, so that the dextromethorphan with low concentration can be accurately detected.

The invention relates to a hybridoma cell strain VAND8, an anti-vanillin monoclonal antibody generated by the hybridoma cell strain VAND8 and application, and belongs to the field of immunochemistry. The hybridoma cell strain VAND8 provided by the invention can be used for preparing a high-titer vanillin-resistant monoclonal antibody, and the titer measured by an enzyme-linked immunosorbent assay (ELISA) method can reach 3.2 * 10 < 5 >; the vanillin-resistant monoclonal antibody provided by the invention is high in sensitivity and good in specificity, the 50% inhibition concentration IC50 on vanillin is 22.1 mu g / mL, and the cross reaction rate with vanillin structural analogues is low or even no cross reaction exists; the method can be used for measuring the content of vanillin.

The invention provides a hybridoma cell strain secreting a napropamide monoclonal antibody and application of the hybridoma cell strain, and relates to the technical field of immunodetection. The monoclonal antibody secreted by the hybridoma cell strain disclosed by the invention has good sensitivity to the napropamide, and the IC50 of the monoclonal antibody disclosed by the invention to the napropamide is 1.13 ng / mL, so that the monoclonal antibody disclosed by the invention can be used for detecting trace napropamide. In addition, the monoclonal antibody disclosed by the invention has no cross reaction on structural analogues of the napropamide, such as alachlor, acetochlor, propachlor, propanil, diquat and diuron, so that the monoclonal antibody disclosed by the invention can be used for accurately detecting the low-concentration napropamide.

The invention belongs to the technical field of biological drug analysis, and particularly relates to a method and a system for quantitatively detecting antibody drugs by using a dimethyl-labeled modified peptide fragment as an internal standard. Sequentially carrying out dimethyl labeling on the enzymolysis peptide fragments of the antibody drugs to obtain a plurality of dimethyl-labeled modified peptide fragments which are used as internal labels; the method comprises the following steps: enriching antibody drugs in a biological sample, and carrying out enzyme digestion to obtain a plurality of unlabeled modified peptide fragments; and mixing the unlabeled modified peptide fragment with an internal standard, enriching the modified peptide fragment, and then carrying out quantitative detection on the modified peptide fragment by adopting liquid chromatography-tandem mass spectrometry. A one-to-one structural analogue internal standard is provided for the modified peptide fragment, and the matrix effect is eliminated. Meanwhile, the method is combined with a high-pH reversed-phase grading equal peptide fragment enrichment method, so that the detection sensitivity of the modified peptide fragments in the matrix is improved. The technology provides a sensitive and reliable quantitative analysis method for the modified peptide fragment, and can be used for quantitative analysis of antibody drugs in biological samples.

The present invention discloses a method for highly selectively extracting vanillin using an ionic liquid / salt / sulfonylated calix[4]arene aqueous two-phase system. n The extract containing vanillin is added to the aqueous two-phase system of mim]Br / Na3C6H5O7 / SC[4], mixed, centrifuged to separate the two phases completely, and the upper phase is collected to obtain the extracted vanillin. n mim]Br / Na3C6H5O7 aqueous two-phase system increases the polarity difference between the two phases, promotes the formation of the two-phase system, improves the distribution coefficient of vanillin in the two-phase system, and can highly selectively extract vanillin from a variety of structural analogs. The method is simple and easy.

The invention relates to the technical field of medicines, and particularly discloses an application of 4-chloro-2, 5-dimethoxy-N-[(2-methoxyphenyl) methyl] phenylethylamine (25C-NBOMe) in preparation of a medicine for inhibiting addiction, and a preparation method of the 4-chloro-2, 5-dimethoxy-N-[(2-methoxyphenyl) methyl] phenylethylamine (25C-NBOMe). As a selective 5-HT2A receptor agonist, the compound can regulate a neural circuit from the prefrontal cortex to the marginal system and indirectly influence dopamine release, so that active drug foraging, relapse and preference behaviors of addicted individuals are inhibited. The invention further covers pharmaceutical salt forms and structural analogues of the compound and application of the compound in pharmaceutical compositions, and administration can be achieved through multiple dosage forms such as sustained-release implants and nasal spray. Compared with the existing drug for treating addiction, the invention has the advantages of independence, definite target spot, obvious curative effect, high safety and the like, is suitable for intervention of various addiction substances, and provides a new strategy for prevention and treatment of addiction behaviors.

The invention relates to a method for specifically detecting tetrahydrocannabinol and hydride thereof by utilizing bridged beta-cyclodextrin, and belongs to the technical field of analysis and detection of supramolecular materials. The method comprises the following steps: firstly, combining 1, 6-hexamethylenediamine bridged bis-beta-cyclodextrin with an indicator to form a supramolecular probe; in a buffer system with a pH value of 9-11, tetrahydrocannabinol or a hydride thereof specifically competes to be combined with a cavity and displaces an indicator by utilizing a synergistic inclusion effect of a bridging subject and dissociation difference of guest molecules, so that the system is subjected to fluorescence quenching or color change, and a structural analogue cannabidiol does not generate obvious interference. The method solves the problems that the sensitivity is low and cannabidiol cannot be effectively distinguished in the prior art through cooperation of specific main body construction and environment regulation, has the advantages of being rapid in detection, high in specificity, high in accuracy and the like, and is suitable for environment monitoring and field screening.

The present invention provides diazo compounds having anti-trypanosomal activity, with less or no side-effects. The diazo compounds are structural analogue of quinapyramine and possess characteristics necessary for the targeted pharmacologic action of anti trypanosomosis, with reduced cytotoxicity. The compounds were found to have less cytotoxicity, and identical or better anti- parasitemia effect as compared to conventional quinapyramine sulfate.

This invention belongs to the field of immunology and discloses a hybridoma cell line and the preparation and application of its secreted monoclonal antibody that uniformly recognizes tylosin / tilmicosin. The monoclonal antibody hybridoma cell line is named DES-AOAA-14D5, with accession number CGMCC NO: 45303. This invention utilizes decarboxymethyl tylosin and oxycarboxymethyl hydroxylamine to synthesize a hapten, conjugates a carrier protein to prepare a complete antigen, immunizes mice, and, through cell fusion and screening techniques, prepares a hybridoma cell line that can secrete a monoclonal antibody that uniformly recognizes tylosin / tilmicosin. IC50 50 The effective concentrations were 1.59 and 1.72 ng / mL, respectively, with a cross-reactivity rate as high as 92.44%. An immunoassay method for the simultaneous and accurate detection of tylosin / tilmicosin in milk was established using this antibody. The cut-off value for both tylosin and tilmicosin was 16 ng / mL, and no cross-reactivity was observed with other structural analogs. The detection method of this invention shows promising application prospects.

The invention discloses a method for automatically identifying new pollutants and structural analogues thereof in environment complex samples, which comprises the following steps of: firstly, analyzing co-occurrence of abundance of substances among samples by using a Pearson's correlation coefficient, and introducing program blank deduction and IQR-Fisher Z transformation abnormal value processing to improve reliability; then, a molecular network is constructed based on high correlation and spectrogram similarity, and cracking products in the source are filtered. Key points are as follows: an iterative annotation strategy is adopted: a candidate structure is obtained from a PubChem database according to m / z of a substance to be annotated, preliminary screening is performed by calculating a Tanimoto coefficient of a Morga fingerprint, or advanced annotation is performed in combination with predicted MS / MS spectrogram fragment matching of a Top-k candidate, and the substance which is successfully annotated is taken as a new round of seed compound, so that automatic recursive expansion of an annotation range is realized. According to the method, the dependence of a traditional method on a standard spectrogram is effectively overcome, and efficient and automatic identification of pollutants with novel structures and analogues thereof is realized.

The application belongs to the technical field of chemical analysis and detection, and discloses a zinc oxide quantum dot fluorescent sensor and application of the zinc oxide quantum dot fluorescent sensor in detection of kaempferol. The zinc oxide quantum dot fluorescent sensor is prepared by stirring zinc salt solution and lye uniformly, then adding amino silane and water to carry out sol-gel reaction to prepare a ZnO-QDs fluorescent probe solution; then, a substrate is immersed in the ZnO-QDs fluorescent probe solution to carry out incubation, and drying is carried out to obtain the zinc oxide quantum dot fluorescent sensor. S The zinc oxide quantum dot fluorescent sensor is applied to detection of kaempferol, can produce specific response with kaempferol, makes the fluorescence intensity of the ZnO-QD fluorescent probe gradually increase with the increase of the concentration of kaempferol, and can effectively exclude the interference of structural analogs such as quercetin and luteolin, so that the kaempferol in the sample can be accurately quantified; and the detection cost is low, the operation is convenient, the detection efficiency is high, the detection is green and safe, and the zinc oxide quantum dot fluorescent sensor is suitable for popularization and application.

The application discloses a nucleic acid aptamer OUC-Fx11-5T of fucoxanthin and application thereof, and belongs to the technical field of nucleic acid aptamer. The nucleic acid aptamer OUC-Fx11-5T of fucoxanthin has a nucleotide sequence as shown in SEQ ID NO. 19. The nucleic acid aptamer OUC-Fx11-5T of fucoxanthin is applied to the recognition or detection of fucoxanthin. The nucleic acid aptamer OUC-Fx11-5T of fucoxanthin has an affinity dissociation constant of 68.2 nM with fucoxanthin by isothermal titration calorimetry, has no obvious affinity to structural analogs of fucoxanthin such as violaxanthin, neoxanthin, zeaxanthin and beta-carotene, has high affinity and good specificity to fucoxanthin, and can be used for the recognition or detection of fucoxanthin.

The invention relates to a hybridoma cell strain secreting a mepiquat chloride monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. The hybridoma cell strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 17, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.46517. The monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity to mepiquat chloride, the IC50 value of the mepiquat chloride is 4.981 ng / mL, structural analogues and functional analogues of the mepiquat chloride, such as chlormequat chloride, paclobutrazol and uniconazole, have no cross reaction, the cross reaction rate is smaller than 1%, and the monoclonal antibody secreted by the hybridoma cell strain can be used for preparing the mepiquat chloride monoclonal antibody. Therefore, low-concentration mepiquat chloride can be accurately detected.

The application discloses an anti-cyprodinil monoclonal antibody and application thereof. The light chain variable region of the anti-cyprodinil monoclonal antibody has an amino acid sequence shown in SEQ ID No. 1, and the heavy chain variable region has an amino acid sequence shown in SEQ ID No. 2. The monoclonal antibody has good affinity to cyprodinil, the half-inhibitory concentration (IC 50 ) is 4.55 ng / mL, the detection limit (IC 10 ) is 1.34 ng / mL, the linear range (IC 20 ~ IC 80 ) is 2.11~9.83 ng / mL, the cross-reaction rate of structural analogs such as fenamidone, bifenox and pheonox is less than 1%, the monoclonal antibody has the characteristics of good thermal stability, strong resistance to organic solvents and acid and alkali, high sensitivity, and is suitable for detecting cyprodinil residues in different environments.

The invention belongs to the technical field of organic synthesis, and particularly relates to a preparation method and application of a 2-phenyl-2H-chromene compound. According to the method, benzaldehyde and o-hydroxyacetophenone are taken as initial raw materials, Claisen-Schmidt condensation is carried out under an alkaline condition to prepare 2-hydroxychalcone, NaBH4 is utilized to reduce carbonyl to prepare an intermediate 2-(1-hydroxy-3-phenyl allyl) phenol, and then in an alkaline environment created by organic alkali Et3N, a 2-phenyl-2H-chromene skeleton is constructed by utilizing the complexing effect of BTC for the first time. According to the synthesis route, the synthesis efficiency of the chromene is remarkably improved, a practical method is provided for rapidly constructing a 2-phenyl-2H-chromene skeleton, and an important foundation is laid for efficient synthesis and structure-function relationship research of the chromene and structural analogues thereof.

The invention provides a conformation-locked amphibian skin peptide derivative or a pharmaceutically acceptable salt thereof. The conformation-locked amphibian skin peptide derivative or the pharmaceutically acceptable salt thereof has a structural formula shown in the specification or a structural analogue thereof, x is derived from one amino acid in TLa, the sequence of the TLa is LLRHVVKILEKYL, and (X) 3 is composed of any continuous sequences in three TLa. The invention also provides a preparation method of the tumor microenvironment response type TLa derivative-STING agonist MSA-2 conjugate and an application of the tumor microenvironment response type TLa derivative-STING agonist MSA-2 conjugate in tumor immunotherapy. In-vivo experiments show that the conjugate can effectively inhibit primary tumor growth and distal pulmonary metastasis in a melanoma model, and tumors of part of mice can completely fade away. The conjugate disclosed by the invention is expected to be developed into a novel efficient and low-toxicity tumor immunotherapy drug.

The invention belongs to the technical field of beta-carotene treatment, and discloses a purification method of beta-carotene fermentation broth, which comprises the following steps: S1, pretreatment: adding a composite antioxidant with the mass percentage of 0.01%-0.1% into the fermentation broth, and then removing thalli and large-particle impurities through centrifugation or microfiltration; and S2, carrying out supercritical CO2 extraction. By integrating supercritical CO2 extraction, molecular imprinting adsorption, multi-stage membrane separation, programmed cooling crystallization and other technologies, the method has the following remarkable advantages that on one hand, efficient separation and purification are achieved, the selective separation efficiency of beta-carotene is remarkably improved through the synergistic effect of the supercritical CO2 extraction and molecular imprinting technologies, and the separation efficiency of beta-carotene is greatly improved; the impurities of structural analogues such as carotenoid in the fermentation liquor are effectively removed; on the other hand, the impurities with different molecular weights are accurately removed through the graded purification effect of the multi-stage membrane separation system, and the product purity is greatly improved.

The invention discloses a method for synchronously detecting quaternary ammonium salts with different polarities and structural analogues thereof in high-organic-matter soil / sediment. The method comprises the following steps: performing freeze drying and low-temperature homogeneous grinding on a soil or sediment matrix sample, adding an internal standard substance and an extracting agent, performing vortex mixing, performing ultrasonic extraction, and performing low-temperature and high-speed centrifugation to obtain an extracting solution; heating in a water bath, carrying out nitrogen blowing concentration on an extracting solution until the extracting solution is nearly dry to obtain a concentrated solution, carrying out purification, leaching and impurity removal on the concentrated solution in a methanol-activated solid-phase extraction column, concentrating an eluent, adding a recovery rate indicator, and fixing the volume to obtain a sample to be detected; and detecting the sample to be detected by adopting an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. According to the method, quaternary ammonium salts with different polarities and structural analogues thereof can be accurately quantified, and compared with a traditional method, the pollutant detection class group is wider, the recovery rate of substances to be detected is high, and the stability is higher.

The application discloses a hapten of abamectin B1a and / or its structural analog, an artificial antigen and application thereof. The application connects a spacer to abamectin after hydrolysis to obtain a hapten of abamectin B1a and / or its structural analog, and then connects a carrier protein to prepare an artificial antigen, and further prepare a monoclonal antibody. A chemiluminescence immunoassay method is established according to specific recognition of the antibody and the antigen, and abamectin residues in a sample are detected. The method can be used for trace detection of abamectin residues, and can avoid a complicated pretreatment step. The detection limit is 0.03 ng / mL, the IC 50 (50% inhibitory concentration) is 0.1 ng / mL, and the linear range IC 20 ~IC 80 is 0.04-0.20 ng / mL.

Provided is use of kynurenic acid in resisting hyperuricemia and a structural analog thereof in preparing an XOD inhibitor, wherein kynurenic acid and the structural analog have good inhibitory activity on xanthine oxidase (XOD). Kynurenic acid has no significant influence on the growth and body weight of mice, and the oral administration of kynurenic acid at a low dose, medium dose, or high dose can significantly reduce the serum levels of creatinine and blood urea nitrogen in mice and effectively inhibit the activity of XOD in the liver of mice, thus reducing the level of uric acid in the livers in a concentration-dependent manner. Kynurenic acid, whether administered at a low dose, medium, or high dose, can effectively reduce the enzymatic activity of adenosine deaminase (ADA) in the serum of mice with hyperuricemia and limit the generation of uric acid precursors. Therefore, kynurenic acid can be used as a medicament for alleviating or treating hyperuricemia.

The invention discloses a method for detecting the plasma concentration of polymyxin E. L-PME1 is used as an internal standard, a liquid chromatography-tandem mass spectrometry detection method is adopted for detection, the extraction efficiency and the mass spectrometry ionization efficiency are far superior to those of a common structural analogue internal standard, and a high internal standard synchronization rate can be kept in different matrixes. In addition, the L-PME1 does not exist in a natural polymyxa fermentation product, so that cross influence cannot be generated.

The invention discloses a chloramphenicol hapten, antigen, antibody and colloidal gold detection device as well as a preparation method and application thereof. According to the chloramphenicol hapten prepared by using a total synthesis method, all potential recognition sites of chloramphenicol are reserved to the maximum extent, particularly, the most critical dichloro structure of chloramphenicol is reserved for the first time, after a spacer arm is introduced, the distribution of electron cloud of the hapten is almost consistent with that of overall electron cloud of chloramphenicol, and the immunogenicity of the chloramphenicol antigen is improved; the chloramphenicol antigen and the monoclonal antibody prepared by the invention are high in specificity when being used for ELISA detection, the IC50 value is 8.21 pg / mL, the linear detection range is 1.71-39.45 pg / mL, and the cross reaction rates of the chloramphenicol antigen and the monoclonal antibody to common structural analogues are lower than 0.01%; and the sensitivity to chloramphenicol in a sample is 0.1 g / kg.

Structural analogs of 2-methyl-2-alkoxy-propane are disclosed, and more particularly structural analogs of l,l,l,3,3,3-hexafluoro-2-trifluoromethyl-2-alkoxy-propane finding usefulness in integrated circuit manufacturing processes, are disclosed. Mixtures configured to etch a thin film of an integrated circuit material comprising structural analogs of l,l,l,3,3,3-hexafluoro-2-trifluoromethyl-2-alkoxy-propane are disclosed too. This application additionally describes methods to etch the thin film of the integrated circuit material utilizing the structural analogs of 1,1,1,3,3,3- hexafluoro-2-trifluoromethyl-2-alkoxy-propane described above, and utilization of these compounds in integrated circuit patterning processes too.

The invention relates to fluorescent metal organic gel Zr-TCBPE-MOG as well as a preparation method and application of the fluorescent metal organic gel Zr-TCBPE-MOG. The preparation method of the organic gel Zr-TCBPE-MOG comprises the following steps: dissolving zirconium chloride, 1, 1, 2, 2-tetra (4-carboxybenzene) ethylene and acetic acid in N, N-dimethylformamide, and then heating and cooling the mixture to obtain light yellow gel, namely the Zr-TCBPE-MOG. According to the invention, the synthesized fluorescent metal organic gel Zr-TCBPE-MOG is used as a fluorescent probe, piceatannol is successfully and specifically recognized from five structural analogues of piceatannol, pterostilbene, pinocembrin, Danjie rheum emodin and resveratrol, and according to the remarkable fluorescence quenching effect of piceatannol on the MOG material, the fluorescence intensity of the MOG material can be effectively improved. The piceatannol fluorescence detection method which is simple, convenient, rapid, ultra-sensitive and high in selectivity is successfully established.

The application provides a hybridoma cell strain secreting monoclone antibody of diquat and application thereof, and relates to the technical field of immune detection. The monoclone antibody secreted by the hybridoma cell strain has good sensitivity to diquat, the IC 50 1.13 ng / mL, so that the monoclone antibody can detect trace diquat. In addition, the monoclone antibody has no cross reaction to structural analogs of diquat, such as alachlor, acetochlor, butachlor, propanil, diquat and diquat, so that the monoclone antibody can accurately detect low concentration diquat.

The invention discloses a cefalonium hapten, an antigen, an antibody as well as a preparation method and application thereof, and relates to the cefalonium hapten, the antigen, the antibody as well as the preparation method thereof and the application in detecting cefalonium residues. The cefalonium hapten and the artificial antigen as shown in the formula (I) are prepared by using a total synthesis method, and a specific antibody for detecting cefalonium is further prepared. The cross reaction rate of the antibody prepared from the hapten / antigen to common structural analogues is lower than 0.01%, the accuracy is high, the interference of other structural analogue drugs can be effectively eliminated, and a basic raw material is provided for subsequent further development of an immune rapid detection technology and a product aiming at cefalonium. # imgabs0 # is represented by formula (I).