Kit for magnetic particle chemiluminescence immunodetection of tyrosine phosphatase autoantibody, preparation method and detection method

Abstract

The invention provides a kit for magnetic particle chemiluminescence immunodetection of a tyrosine phosphatase autoantibody, a preparation method and a detection method. The kit comprises: a magnetic particle-anti-biotin antibody solution, a tyrosine phosphatase antigen solution labeled by biotin and an anti-tyrosine phosphatase antibody solution labeled by enzyme. According to the kit for magnetic particle chemiluminescence immunodetection of a tyrosine phosphatase autoantibody, the preparation method and the detection method provided by the invention, chemiluminescence immunoassay and a magnetic particle technology are combined, so that the detection sensitivity and accuracy are greatly improved; the kit has the characteristics of high sensitivity, good specificity, high stability, strong applicability, low experiment cost and the like, and meanwhile, the antibody is coated by taking magnetic particles as a solid-phase carrier, so that the antigen-antibody contact area and the luminous area are increased, and the reaction sensitivity is improved; the reaction is carried out in a liquid phase, and magnetic particles have a stirring effect by utilizing a rotating magnetic field, so that the reaction time is greatly shortened.

Description

technical field [0001] The disclosure relates to the technical field of immunoassay and detection, in particular to a kit for chemiluminescent immunodetection of tyrosine phosphatase autoantibody magnetic particles, a preparation method and a detection method. Background technique [0002] Diabetes mellitus is a chronic and complex metabolic disease characterized by hyperglycemia and glucose metabolism disorder. In 2007, there were about 246 million people with diabetes in the 20-79 age group in the world, accounting for 5.9% of the global population. China is the hardest-hit area for diabetes. Not only does the number of people with diabetes account for nearly 1 / 6 of the world, but it also grows rapidly. Diabetes has brought a heavy economic burden to society and families, and it is a kind of physical and mental devastation to individual patients. Since the etiology and pathogenesis of diabetes have not been fully elucidated, it has brought many difficulties to the preven...

AI Technical Summary

Problems solved by technology

[0005] The current tyrosine phosphatase antibody detection technology has the following disadvantages: high detection cost, low detection sensitivity, narrow detection linear range, many factors affecting the measured value, radioactive contamination, complicated operation, etc.

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