41results about How to "Have immune response properties" patented technology

The invention belongs to the field of biotechnology and relates to an antigenic mimic epitope of gentamycin, of which the amino acid sequence is WHWQWWLQTDAT. The antigenic mimic epitope of gentamycin can replace an expensive and strong-toxicity gentamycin standard substance and serve as a competitive antigen or a solid coat antigen to be applied to immunological detection of gentamycin. The antigenic mimic epitope of gentamycin has immune reaction characteristics similar to that of natural gentamycin molecules, has a very good effect, reduces harm of gentamycin to the human health, saves the cost and has a very high application value.

The invention belongs to the biological technical field, and in particular relates to preparation and application of a zearalenone anti-idiotype nano antibody, which has an amino acid sequence SEQ ID No.1, also relates to the nucleotides encoding the amino acid. The nano antibody of the invention can replace the expensive toxic Zen standard, and can be used in immunological detection of Zen as a competitive antigen or solid coating antigen. the nano antibody has similar immunological properties to natural Zen molecule, and good effect. Compared to a traditional antigen mimicking epitope based on polypeptide and a traditional anti-idiotype antibody based on IgG, the nano antibody has the advantages of more stable structure, acid resistance, alkali resistance, high temperature resistance and high detection sensitivity, so that the immune detection stability has been greatly improved, and the tolerance on the environment has also been improved.

The invention provides an enrofloxacin-mimic antigen epitope peptide, and preparation methods and an application thereof. The ENR-mimic antigen epitope peptide having a sequence represented by GKPYITWis panned from a phage random display heptapeptide library through a phage display peptide library technology with an anti-ENR monoclonal antibody as a target. The invention respectively provides a method for preparing the antigen-mimic epitope by a phage amplification technology and a method for preparing the antigen-mimic epitope by a gene recombination technology. A concrete detection method is designed based on enzyme linked immunosorbent assay, colloidal gold immunochromatography and fluorescence microsphere immunochromatography by using the immunological binding property of the antigen-mimic epitope. The antigen-mimic epitope has similar immunoreactivity characteristics to natural ENR molecules, can be used in the immunological detection of ENR to replace an ENR standard substance having strong drug resistance and high price, and the detection result is accurate and highly-sensitive.

The invention discloses a CD105 nanometer antibody for a CD105 polypeptide molecular epitope, further discloses a gene sequence for encoding the CD105 nanometer antibody and an expression carrier and a host cell which can express the CD105 nanometer antibody, and discloses application of the CD105 nanometer antibody. According to the CD105 nanometer antibody, the gene sequence, the host cell and the like, the CD105 nanometer antibody can be efficiently expressed inside escherichia coli, and the CD105 nanometer antibody is good in CD105 immunoreaction specificity, high in affinity and capable of being applied to preparation of CD105 detection reagents or antitumor drugs or the like.

The invention belongs to the biological technical field, and in particular to preparation and application of nano antibody capable of specifically combining with anti-zearalenone antibody and with an amino acid sequence of SEQ ID No.1; and the invention also relates to the nucleotides encoding the amino acid. The nano antibody of the invention can replace the expensive toxic Zen standard, and can be used in immunological detection of Zen as a competitive antigen or solid coating antigen. The nano antibody has similar immunological properties to natural Zen molecule, and good effect. Compared to a traditional antigen mimicking epitope based on polypeptide and a traditional anti-idiotype antibody based on IgG, the nano antibody has the advantages of more stable structure, acid resistance, alkali resistance, high temperature resistance and high detection sensitivity, so that the immune detection stability has been greatly improved, and the tolerance on the environment has also been improved.

The invention belongs to the field of biotechnology, and specifically relates to the preparation and application of a nanobody that can specifically bind to an anti-deoxynivalenol antibody. Its amino acid sequence is SEQ ID NO.: 1, and it also relates to a nucleotide encoding the amino acid. The nanobody of the present invention can replace the expensive and highly toxic DON standard product, and can be used as a competitive antigen or solid-phase coated antigen in the immunological detection of DON. The nanobody has similar immune response characteristics to natural DON molecules, and the effect is good. . Compared with traditional polypeptide-based antigen mimic epitopes and IgG-based traditional anti-idiotypic antibodies, nanobodies have more stable structures, acid, alkali and high temperature resistance, and high detection sensitivity, so their immunological detection stability has been greatly improved. At the same time, the tolerance to the environment has also been improved.

The invention belongs to the field of biotechnologies and relates to an antigenic mimic epitope of bisphenol A. The amino acid sequence of the antigenic mimic epitope is YPPHNWDYSGAA. The antigenic mimic epitope of bisphenol A, disclosed by the invention, can replace a bisphenol A standard which is expensive in price and strong in toxicity, is applied to the immunological detection of bisphenol A as a competitive antigen or solid-phase coating antigen, has the immunoreaction characteristics similar to those of natural bisphenol A molecules and has a very good effect, so that the injury to the health of a human body caused by bisphenol A is reduced, the cost is reduced, and the application value is very high.

The invention discloses a CD105 nano antibody aiming at CD105 polypeptide molecular epitope, further discloses a coding gene sequence of the CD105 nano antibody, a CD105 nano antibody expression carrier and a host cell and also discloses application of the CD105 nano antibody. By the CD105 nano antibody, the gene sequence, the host cell and the like, efficient expression of the CD105 nano antibody in escherichia coli can be realized, high specificity in immunoreactions with CD105 and high affinity can be realized, and application to preparation of CD105 detection agents, antitumor medicines or the like is realized.

The invention belongs to the biotechnology field and relates to an antigen mimic epitope of aflatoxin B1. The amino acid sequence of the antigen mimic epitope is WPLYYYPWSDGA. The antigen mimic epitope of the aflatoxin B1 can substitute standard aflatoxin B1 with high price and strong toxicity, and is applied to the immunological detection of the aflatoxin B1 as a competition antigen or a solid phase envelope antigen; the antigen mimic epitope has the immunoreaction characteristic similar to the natural aflatoxin B1 molecule, has good effect, can reduce the harm of the aflatoxin B1 to human health, saves the cost and has high application value.

The invention discloses a CD105 nano antibody aiming at CD105 polypeptide molecular epitope, further discloses a coding gene sequence of the CD105 nano antibody, a CD105 nano antibody expression carrier and a host cell and also discloses application of the CD105 nano antibody. By the CD105 nano antibody, the gene sequence, the host cell and the like, efficient expression of the CD105 nano antibody in escherichia coli can be realized, high specificity in immunoreactions with CD105 and high affinity can be realized, and application to preparation of CD105 detection agents, antitumor medicines or the like is realized.

The invention belongs to the field of biotechnology and relates to an antigen mimic epitope of norfloxacin, the amino acid sequence of which is DHAYWPKFWGKA. Norfloxacin antigen mimetic epitope of the present invention can replace expensive and highly toxic norfloxacin standard substance, and be used as competition antigen or solid-phase coating antigen to the immunological detection of norfloxacin, this antigen mimetic table The bit has similar immune response properties to the natural norfloxacin molecule and works very well. The harm of norfloxacin to human health is reduced, the cost is saved, and the invention has high application value.

The invention belongs to the biological technical field, and in particular to preparation and application of nano antibody capable of specifically combining with anti-zearalenone antibody and with an amino acid sequence of SEQ ID No.1; and the invention also relates to the nucleotides encoding the amino acid. The nano antibody of the invention can replace the expensive toxic Zen standard, and can be used in immunological detection of Zen as a competitive antigen or solid coating antigen. The nano antibody has similar immunological properties to natural Zen molecule, and good effect. Compared to a traditional antigen mimicking epitope based on polypeptide and a traditional anti-idiotype antibody based on IgG, the nano antibody has the advantages of more stable structure, acid resistance, alkali resistance, high temperature resistance and high detection sensitivity, so that the immune detection stability has been greatly improved, and the tolerance on the environment has also been improved.

The invention belongs to the field of biotechnologies and relates to an antigenic mimic epitope of bisphenol A. The amino acid sequence of the antigenic mimic epitope is YPWIPDRYGSPI. The antigenic mimic epitope of bisphenol A, disclosed by the invention, can replace a bisphenol A standard which is expensive in price and strong in toxicity, is applied to the immunological detection of bisphenol A as a competitive antigen or solid-phase coating antigen, has the immunoreaction characteristics similar to those of natural bisphenol A molecules and has a very good effect, so that the injury to the health of a human body caused by bisphenol A is reduced, the cost is reduced, and the application value is very high.

The invention belongs to the field of biotechnologies and relates to an antigenic mimic epitope of bisphenol A. The amino acid sequence of the antigenic mimic epitope is YPPHNWDYSGAA. The antigenic mimic epitope of bisphenol A, disclosed by the invention, can replace a bisphenol A standard which is expensive in price and strong in toxicity, is applied to the immunological detection of bisphenol A as a competitive antigen or solid-phase coating antigen, has the immunoreaction characteristics similar to those of natural bisphenol A molecules and has a very good effect, so that the injury to the health of a human body caused by bisphenol A is reduced, the cost is reduced, and the application value is very high.

The invention belongs to the field of biotechnology and relates to 7-peptide mimic antigen epitope of norfloxacin, and the amino acid sequence of the mimic epitope is CNTARWPFC. The 7-peptide mimic antigen epitope of norfloxacin can be used for replacing a norfloxacin standard with high cost and toxicity, serves as a competition antigen or a solid-phase coating antigen to be applied to immunological detection of norfloxacin, has immune response characteristics similar to natural norfloxacin molecules, and has a very good effect. Harm of norfloxacin to human bodies is reduced, the cost is saved, and a very high application value is provided.

The invention belongs to the biological technical field and relates to an antigenic mimic epitope C of norfloxacin. An amino acid sequence of the antigenic mimic epitope C of norfloxacin is DHAYWPKFWGKA. The antigenic mimic epitope C of norfloxacin can replace standard norfloxacin with high price and strong toxicity, is applied to immunological detection of norfloxacin as competitive antigen or solid phase envelope antigen and has immunoreaction characteristic similar to that of natural norfloxacin and good effect; harm of norfloxacin to human health is reduced, and cost is saved, so that the antigenic mimic epitope C of norfloxacin has high application value.

The invention discloses a CD105 nano antibody aiming at CD105 polypeptide molecular epitope, further discloses a coding gene sequence of the CD105 nano antibody, a CD105 nano antibody expression carrier and a host cell and also discloses application of the CD105 nano antibody. By the CD105 nano antibody, the gene sequence, the host cell and the like, efficient expression of the CD105 nano antibody in escherichia coli can be realized, high specificity in immunoreactions with CD105 and high affinity can be realized, and application to preparation of CD105 detection agents, antitumor medicines or the like is realized.

The invention provides an enrofloxacin-mimic antigen epitope peptide, and preparation methods and an application thereof. The ENR-mimic antigen epitope peptide having a sequence represented by GKPYITWis panned from a phage random display heptapeptide library through a phage display peptide library technology with an anti-ENR monoclonal antibody as a target. The invention respectively provides a method for preparing the antigen-mimic epitope by a phage amplification technology and a method for preparing the antigen-mimic epitope by a gene recombination technology. A concrete detection method is designed based on enzyme linked immunosorbent assay, colloidal gold immunochromatography and fluorescence microsphere immunochromatography by using the immunological binding property of the antigen-mimic epitope. The antigen-mimic epitope has similar immunoreactivity characteristics to natural ENR molecules, can be used in the immunological detection of ENR to replace an ENR standard substance having strong drug resistance and high price, and the detection result is accurate and highly-sensitive.

The invention belongs to the field of biotechnologies and relates to an antigenic mimic epitope of bisphenol A. The amino acid sequence of the antigenic mimic epitope is YPAYTYNNLMHN. The antigenic mimic epitope of bisphenol A, disclosed by the invention, can replace a bisphenol A standard which is expensive in price and strong in toxicity, is applied to the immunological detection of bisphenol A as a competitive antigen or solid-phase coating antigen, has the immunoreaction characteristics similar to those of natural bisphenol A molecules and has a very good effect, so that the injury to the health of a human body caused by bisphenol A is reduced, the cost is reduced, and the application value is very high.

The invention belongs to the field of biotechnology and relates to an antigenic mimic epitope of aflatoxin B1. The amino acid sequence of the antigenic mimic epitope is CDPRKHIHC or VPYSPHYFERMI. The antigenic mimic epitope of aflatoxin B1 can replace an expensive AFB1 standard substance with strong toxicity and be applied to an immunological detection of AFB1 as a competitive antigen or a solid phase envelope antigen. The antigenic mimic epitope has immunoreaction characteristics similar to natural AFB1 molecules, and has good effects. Harm to human health caused by AFB1 is reduced, the cost is saved, and the antigenic mimic epitope of AFB1 has great application value.

The invention discloses a CD105 nano antibody aiming at CD105 polypeptide molecular epitope, further discloses a coding gene sequence of the CD105 nano antibody, a CD105 nano antibody expression carrier and a host cell and also discloses application of the CD105 nano antibody. By the CD105 nano antibody, the gene sequence, the host cell and the like, efficient expression of the CD105 nano antibody in escherichia coli can be realized, high specificity in immunoreactions with CD105 and high affinity can be realized, and application to preparation of CD105 detection agents, antitumor medicines or the like is realized.

The invention belongs to the field of biotechnology and relates to an antigenic mimic epitope of aflatoxin B1. The amino acid sequence of the antigenic mimic epitope is CDPRKHIHC. The antigenic mimic epitope of aflatoxin B1 can replace an expensive AFB1 standard substance with strong toxicity and be applied to an immunological detection of AFB1 as a competitive antigen or a solid phase envelope antigen. The antigenic mimic epitope has immunoreaction characteristics similar to natural AFB1 molecules, and has good effects. Harm to human health caused by AFB1 is reduced, the cost is saved, and the antigenic mimic epitope of AFB1 has great application value.