Enrofloxacin-mimic antigen epitope peptide, and preparation method and application thereof
A technology of antigenic epitopes and mimetic epitopes, which is applied in the direction of analyzing materials through chemical reactions, observing the influence of chemical indicators for material analysis, and material inspection products, etc., which can solve the immunological properties of antigenic epitope peptides Adaptability matching, difficult to obtain detection results, unclear preparation methods and other problems, to achieve high sensitivity, reduce harm to human health and ecological environment, and highlight the effect of application value
Active Publication Date: 2018-08-10
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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Abstract
The invention provides an enrofloxacin-mimic antigen epitope peptide, and preparation methods and an application thereof. The ENR-mimic antigen epitope peptide having a sequence represented by GKPYITWis panned from a phage random display heptapeptide library through a phage display peptide library technology with an anti-ENR monoclonal antibody as a target. The invention respectively provides a method for preparing the antigen-mimic epitope by a phage amplification technology and a method for preparing the antigen-mimic epitope by a gene recombination technology. A concrete detection method is designed based on enzyme linked immunosorbent assay, colloidal gold immunochromatography and fluorescence microsphere immunochromatography by using the immunological binding property of the antigen-mimic epitope. The antigen-mimic epitope has similar immunoreactivity characteristics to natural ENR molecules, can be used in the immunological detection of ENR to replace an ENR standard substance having strong drug resistance and high price, and the detection result is accurate and highly-sensitive.
Application Domain
Material analysis by observing effect on chemical indicatorBiological testing
Technology Topic
MimotopeImmunosorbent method +12
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