The invention belongs to the technical field of preparation of probiotics, and provides a
bacterial strain with anti-allergic and
immunity-improving effects and application of the
bacterial strain. According to the method, a three-layer physical self-
assembly structure is adopted, and
polysaccharide-
polyphenol layer-by-layer self-
assembly coating treatment, lipid
insertion layer treatment and
calcium ion crosslinking protection
coating treatment are sequentially carried out. And
mannan oligosaccharide MOS and / or
galactooligosaccharide GOS are / is mixed in the
chitosan layer in a ratio of 0.05-0.15% w / v, and the surface
sugar ligand density reaches 4-8 [mu] g per 10 CFU. The
coating is constructed under the condition that the pH value is 5.4-5.8; the thickness of the lipid layer is 20-80 nm when the pH value is 5.6-6.2; the cross-linked layer is treated at the pH of 6.0-6.5, so that the
gastric acid environment is stable for 2 hours, and the
gastric acid is controllably released in
intestinal juice for 2-4 hours. The prepared strain has the
total thickness of 220-900 nm, the outermost layer coverage rate is larger than or equal to 85%, the gastric juice
survival rate is remarkably improved, the strain can be prepared into an oral preparation to be used for preventing and relieving allergic diseases, the
viable count of each
dose is 1 * 10 <-1 > * 10 <-1 > CFU, and the process is mild and suitable for industrial production.