39 results about "Succus entericus" patented technology

The invention provides bacillus subtilis (Bacillussubtilis) shou003, an anti-vibrio protein and a preparation method and applications of the bacillus subtilis shou003 and the anti-vibrio protein. The preparation method of the anti-vibrio protein comprises the following steps: screening out bacillus subtilis shou003 (preserved in China Center for Type Culture Collection with a number of CCTCC No.: M2013571 on November 13, 2013) from intestinal tract of a healthy large yellow croaker; and then extracting the anti-vibrio protein from the fermentation broth of bacillus subtilis shou003, wherein the amino acid sequence of the anti-vibrio protein is shown as SEQ ID No.: 1. The bacillus subtilis shou003 and the anti-vibrio protein show good effects on inhibiting aquatic pathogenic bacteria, in particular pathogenic vibrio; in addition, the bacillus subtilis shou003 shows outstanding tolerance to temperature, NaCl, gastric juice, intestinal juice and cholate and can be widely applied to the prevention of germs during aquaculture; on that basis, the optimal fermentation culture method of the bacillus subtilis shou003, and a preparation method of the anti-vibrio protein are provided.

The invention relates to a probiotics microcapsule and a preparation method thereof. The preparation method comprises the steps of probiotic strain activation, fungus milk mixed solution preparation, microcapsule preparation, cooling drying and the like. The embedding rate of the probiotics microcapsule finished product prepared by the method disclosed by the invention is over 76 percent, and the number of living bacteria is larger than 10<8>CFU/g; the particles are uniform, and the average particle size is about 100 microns; the number of probiotics living bacteria is only reduced by 0.22 (Log10CFU/g) after heat preservation in artificially simulated gastric fluid with the temperature of 37 DEG C for 2.5 hours; probiotics in the microcapsule is basically released totally after heat preservation in artificially simulated succus entericus with the temperature of 37 DEG C for 90min, and the number of living bacteria can be up to 8.810+/-0.548 (Log10CFU/g); after freeze drying, the survival rate of probiotics is up to 64.733+/-5.221 percent; and after the microcapsule is stored within the temperature range of 4-37 DEG C for 28 days, the number of probiotics living bacteria is larger than 10<7>CFU/g.

The invention discloses a production method of enteric-coated kitasamycin for feed, which comprises the following process steps: step one, the preparation of drug-loaded pellets; step two, inner layer sustained-release coating of the drug-loaded pellets, which prolongs the release and acting time of kitasamycin; and steps three, outer layer enteric coating of the drug-loaded pellets, which ensures the release in succus entericus and small or no release in gastric juice. The method has the advantages that the kitasamycin is subjected to pellets pelletizing and 99 percent of the prepared granulums can pass through 24 meshes, so the dust is greatly reduced and the fluidity is increased; the coating of the inner layer sustained-release agent (HPMC) prolongs the release and acting time of the kitasamycin, reduces medication times and reduces the medication cost; and a layer of enteric substance, namely acrylic resin-III is sprayed and coated on the outer layer of particles. The substance protects the kitasamycin which is a weakly alkaline antibiotic from being damaged by gastroc acid in stomach, quickly disintegrates after entering enteric canal and releases the kitasamycin; and then the kitasamycin is absorbed by gastrointestinal mucosa into blood drug to play a role of restraining the reproduction of pathogenic microorganism and preventing diarrhea. Insoluble in the stomach, kitasamycin coating formulations have no pessimal stimulation on the stomach, and cannot result in regurgitation and vomiting. In addition, the sustained-release formulation, namely the kitasamycin prolongs the acting time so the medication times is reduced, the medication cost of farmers is reduced, and the economic benefit is improved.

The present invention discloses microcapsule for aquiculture and features that the microcapsule consists of Luchong insect powder 50-80 wt%, omega fatty acid 1-5 wt%, fish meal 8-25 wt%, premixed material 3-5 wt%, and glutin and arabic gum as enveloping material 10-25 wt%. The microcapsule contains balanced nutrients and no antibiotic and chitin, and is less water soluble and easy to be digested and absorbed in intestinal. It is one kind of green nutritive material for aquiculture.

The invention discloses a docetaxel solid lipid nanoparticle lyophilized preparation and a preparation method. The quaternary ammonium salt chitosan-modified docetaxel solid lipid nanoparticle lyophilized preparation comprises the following components in parts by weight: 1 part of docetaxel, 20-50 parts of lipid materials, 10-25 parts of emulsifier, 3-7 parts of quaternary ammonium salt chitosan and 150-200 parts of lyophilization protecting agent. The preparation method comprises the following steps: 1) preparation of an oil phase; 2) preparation of diffusion liquid; 3) preparation of nanoparticle suspension; 4) modification of nanotparticles; and 5) lyophilized drying. The quaternary ammonium salt chitosan-modified docetaxel solid lipid nanoparticle has pH universality, the dissolution speed of the medicament can be effectively increased, the problem that the traditional chitosan modified docetaxel solid lipid nanoparticles are instable in succus entericus can be improved, the oral bioavailability can be improved, and the clinical application of the docetaxel solid lipid nanoparticle lyophilized preparation becomes possible.

The invention provides a preparation method of novel salmonella bacteriophage microencapsulated microspheres. The method comprises the following steps of mixing a cationic etherified starch solution and a bacteriophage suspension, and uniformly stirring to obtain a first mixed solution; uniformly stirring and mixing the first mixed solution, a sodium alginate solution, a xanthan gum solution and a nano TiO2 solution to obtain a second mixed solution; and dropwise adding the second mixed solution into a calcium chloride solution to form calcium alginate gel, filtering and collecting bacteriophage microspheres, putting the bacteriophage microspheres into a cationic chitosan oligosaccharide solution, coating for 30 minutes, and filtering and collecting the bacteriophage microencapsulated microspheres. In actual use, after the bacteriophage microspheres enter gastric juice, the bacteriophage surface coated material can effectively resist erosion of gastric acid and prolong the survival time of bacteriophage, and after the bacteriophage microspheres enter intestinal juice, the bacteriophage surface coated material can react with the intestinal juice and is cracked to release the bacteriophage in the material so as to achieve the sterilization effect. The method has the advantages that microencapsulation preparation is carried out at room temperature, the process is simple, the application range is wide, and the cost is low; and the raw materials are wide in source, cheap and easy to obtain, and the bacteriophage in the product is high in activity and encapsulation efficiency and can be directly applied to feed addition.

The invention relates to a lactococcus lactis subsp.hordniae having the high antioxidant activity. The lactococcus lactis subsp.hordniae is lactococcus lactis subsp.hordniae JYZ4. According to the invention, the lactobacillus is preserved in China General Microbiological Culture Collection Center at a preservation site being the Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1,West Beichen Road, Chaoyang District, Beijing and has a preservation number of CGMCC No.17892 on a preservation date of June 3, 2019. The lactobacillus is separated from soy sauce residues and the in-vitro probiotic characteristic research shows that the lactobacillus is resistant to acid, artificial gastric juice and intestinal juice and has high hydrophobicity and antioxidant activity. The lactococcus lactis subsp.hordniae not only can be applied to fermentation of soy sauce residues to make the fodder with high palatability but also can be combined with streptococcus thermophilus, lactobacillus bulgaricus to ferment yoghourt and the like and has broad application prospects and good commercial value.

Antibiotics have long been used at sub-therapeutic levels to control incidences of post-weaning diarrhea and to improve growth performance in pigs. However, the current trend world-wide is to eliminate the use of in-feed antibiotics due to increased public concerns over the spread of antibiotic resistance in bacterial pathogens, which poses a threat to public health. Alternatives to in-feed antibiotics are needed. Thymol essential oil exhibits strong in vitro antibacterial activity; however; direct inclusion of essential oils to pig feeds has limited efficacy due to their high volatility, low stability during feed processing, interactions with other feed components and poor availability in lower gut. To solve these problems, we developed lipid matrix beads using thymol and a fatty acid with incorporation of 2% polysaccharides via a melt-granulation technique. Laurie acid was identified as a suitable carrier for thymol. In vitro release of thymol from lipid matrix granules was determined using simulated salivary fluid (SSF), simulated gastric fluid (SGF) and simulated intestinal fluid (SIF), respectively. The lipid matrix granules with 2% polysaccharides exhibited a slow release rate (%) of essential oil and fatty acid in SSF (21.2±2.3; 36±1.1), SGF (73.7±6.9; 54.8±1.7) and SIF (99.1±1.2; 99.1±0.6), respectively. However, the lipid matrix granules without polysaccharides had quick release (%) of essential oil and fatty acid from the SSF (79.9±11.8; 84.9±9.4), SGF (92.5±3.5; 75.8±5.9) and SIF (93.3±9.4; 93.3±4.6), respectively.

The invention belongs to the technical field of traditional Chinese medicine compound preparation and particularly relates to a preparing method of radix scutellariae-gypsum oral preparation capable of relieving sore throat. The method includes the steps of firstly, extracting volatile oil of gypsum, fructus forsythiae, mint and perilla leaf in a microwave manner; secondly, adding buffalo horn grinding fluid into artificial gastric juice extract and artificial intestinal juice extract; thirdly, jointly decocting fructus forsythiae, mint, perilla leaf and gypsum residues with radix scutellariae, rhizoma anemarrhenae, radix rehmanniae, red peony root, spina gleditsiae, dandelion and liquorice; fourthly, welling mixing the volatile oil clathrate compound and the extract obtained in the first step and the third step, adding the buffalo horn extract in the second step, adding 0.1% of agar and 0.2% of sorbic acid, well mixing, immediately filtering, cooling, adding the volatile oil clathrate compound obtained in the first step, 1% of apple essence and 1.5% of sodium cyclamate, adding distilled water to reach 1000ml, split charging into glass ampoules of 10ml, and sterilizing. The method is simple and easy to operate and stable in experiment results.

The invention relates to the technical field of functional microorganisms, and particularly provides a bacillus coagulans preparation and a preparation method thereof. The bacillus coagulans preparation is prepared by carrying out spray drying on a mixed solution of bacillus coagulans and a coating material, is high in viable count, good in tolerance to artificial gastric acid and intestinal juice and very high in heat resistance, and can be widely applied to the fields of foods, health-care products and the like.

The invention relates to the field of probiotics, in particular to lactobacillus reuteri SXDT-32 and application thereof. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection The strain has a good inhibition effect on escherichia coli and salmonella; the strain can be colonized in an animal body, and has strong tolerance to artificial cholate, artificial gastric acid and artificial intestinal juice; the feed additive can be firmly adhered and colonized in animal intestinal tracts, inhibits growth of pathogenic microorganisms in the intestinal tracts, improves animal intestinal tract immunity, relieves animal colitis, reduces animal diarrhea and death rate, improves animal intestinal tract health, and also can improve animal growth performance.

The invention discloses a fish feed additive containing traditional Chinese medicine ingredients, and relates to the field of fish culture, and the fish feed additive comprises the following components in parts by weight: 10-16 parts of tartaric acid, 8-10 parts of betaine, 7-9 parts of a phagostimulant, 7-10 parts of Toxicodendron vernicifluum leaves, 12-15 parts of zanthoxylum ailanthus, 5-10 parts of licorice, 2.5-4 parts of purslane, 1.5-2 parts of medicated leaven, 3-5 parts of polygonum multiflorum, 2-4 parts of gynostemma pentaphyllum and 20-30 parts of bile acid. The tartaric acid, the phagostimulant, the medicated leaven and the purslane are added into the additive disclosed by the invention, so that the feeding frequency of fishes in sultry weather can be increased, the effect of increasing appetite can be achieved, secretion of gastric juice and intestinal juice is promoted, nutrient absorption of organisms is promoted, nutrient substances are promoted to enter the organisms more quickly, and the food intake of the fishes is increased; the fish feed additive solves the problem that fishes are unwilling to ingest or have a slow ingestion speed in sultry weather, uses Chinese herbal medicines for compatibility, is derived from natural components of plants, is green and environment-friendly, has no toxic effects, and has high social and economic benefits.