23 results about "Succus entericus" patented technology

The invention belongs to the technical field of microbial fermentation, and discloses lactobacillus mucilaginosus and application thereof in preparation of a uric acid reducing preparation. The survival rate of the fermented lactobacillus mucus in simulated gastric juice with the pH value of 2.0 for 3 hours is greater than 92%, the survival rate in simulated intestinal juice environment containing 0.5% of cholate is greater than 86%, and the viability of the fermented lactobacillus mucus is obviously higher than that of a conventional strain; and the tea has the effects of reducing uric acid, resisting oxidation and inhibiting pathogenic bacteria. The xanthine oxidase inhibition rate of the natural medicinal and edible compound potion obtained by mixing and co-fermenting the strain and existing bacterial powder is greater than 77% (45% or more higher than that of an unfermented group), and the oxidation resistance after fermentation can be improved by 50% at the same time. The beverage is suitable for being drunk as a daily functional food for a long time, and provides a natural and healthy collaborative diet relieving scheme for patients with hyperuricemia and gout.

The invention belongs to the technical field of preparation of probiotics, and provides a bacterial strain with anti-allergic and immunity-improving effects and application of the bacterial strain. According to the method, a three-layer physical self-assembly structure is adopted, and polysaccharide-polyphenol layer-by-layer self-assembly coating treatment, lipid insertion layer treatment and calcium ion crosslinking protection coating treatment are sequentially carried out. And mannan oligosaccharide MOS and / or galactooligosaccharide GOS are / is mixed in the chitosan layer in a ratio of 0.05-0.15% w / v, and the surface sugar ligand density reaches 4-8 [mu] g per 10 CFU. The coating is constructed under the condition that the pH value is 5.4-5.8; the thickness of the lipid layer is 20-80 nm when the pH value is 5.6-6.2; the cross-linked layer is treated at the pH of 6.0-6.5, so that the gastric acid environment is stable for 2 hours, and the gastric acid is controllably released in intestinal juice for 2-4 hours. The prepared strain has the total thickness of 220-900 nm, the outermost layer coverage rate is larger than or equal to 85%, the gastric juice survival rate is remarkably improved, the strain can be prepared into an oral preparation to be used for preventing and relieving allergic diseases, the viable count of each dose is 1 * 10 <-1 > * 10 <-1 > CFU, and the process is mild and suitable for industrial production.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LM2 and application of the lactobacillus plantarum strain LM2. The strain has relatively high GABA (gamma-aminobutyric acid) production capacity and nitrite degradation capacity, has a bacteriostatic effect on intestinal pathogens such as staphylococcus aureus, salmonella enteritidis, listeria monocytogenes and campylobacter jejuni, has a good bacteriostatic function, also has good acid resistance and cholate resistance, and can be used for preparing a bacterial strain for treating intestinal diseases. The strain can well survive in artificial intestinal juice and artificial gastric juice; in order to increase the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LM2 is obtained by researching the accelerant, and the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 99% or above, the yield of GABA is increased to 3.0 g / L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

The invention relates to the technical field of microorganisms, in particular to lactobacillus plantarum, selenium-rich lactobacillus plantarum and application thereof. The lactobacillus plantarum with the preservation number of GDMCC No: 64006 is provided, selenium is enriched through the lactobacillus plantarum, and inorganic selenium is converted into biological nano-selenium which can be preserved in vivo for a longer time. The invention provides a technical scheme that lactobacillus plantarum is subjected to selenium-enriched culture to prepare the selenium-enriched lactobacillus plantarum, the elimination capability of the prepared selenium-enriched lactobacillus plantarum to free radicals DPPH and ABTS < + > and the endurance capability of the prepared selenium-enriched lactobacillus plantarum to simulated gastric juice can be remarkably improved, and the tolerance of the prepared selenium-enriched lactobacillus plantarum to cholate and simulated artificial intestinal juice cannot be reduced; meanwhile, the AIEC associated colitis can be relieved from the aspects of accelerating weight recovery, reducing colon tissue pathological damage, reducing AIEC colonization, promoting intestinal barrier repair, reducing the proinflammatory factor level, improving the selenium level, improving intestinal flora disorder and the like.

The present invention relates to the field of microbial technology, and provides a Pediococcus acidilactici YYS-J2 that can degrade acrylamide and produce high levels of phenyllactic acid, and its application. Pediococcus acidilactici The preservation number of YYS-J2 is CGMCC No. 28183. This *Pediococcus lactis* YYS-J2 exhibits excellent acrylamide removal capacity, high efficiency in L-phenylalanine utilization and metabolism, high-quality phenyllactic acid production capacity, and excellent *Streptococcus mutans* antibacterial activity. It also produces superoxide dismutase (SOD) and shows good tolerance in simulated gastric and intestinal fluids. This *Pediococcus lactis* YYS-J2 can provide a new probiotic source for the development of functional products such as acrylamide removal products, antibacterial products, and SOD-producing products, and has significant application value.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LS2 and application thereof.The lactobacillus plantarum strain LS2 is separated from cheese, has the high GABA production capacity and the nitrite degradation capacity, has the antibacterial effect on enteric pathogenic bacteria including escherichia coli, salmonella enteritidis and campylobacter jejuni, and has the advantages that the lactobacillus plantarum strain LS2 is high in GABA production capacity and nitrite degradation capacity; the strain has good antibacterial function, good acid resistance and cholate resistance, and can well survive in artificial intestinal juice and artificial gastric juice; in order to improve the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LS2 is obtained by researching the accelerant, the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 98% or above, the yield of GABA is improved to 3.3 g / L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

The present invention belongs to the technical field of microorganisms, and particularly relates to a Lactobacillus plantarum NXU0011 and application thereof. The Lactobacillus plantarum NXU0011 is preserved in the China General Microbiological Culture Collection Center, with the preservation number of CGMCC NO: 26970. The bacterium has a good inhibition effect on Escherichia coli and Staphylococcus aureus. The Lactobacillus plantarum NXU0011 also has good antioxidant performance, and can be used for preparing hypoglycaemic drugs. At the same time, the Lactobacillus plantarum NXU0011 has a high survival rate in saliva, gastric juice or intestinal juice, and has the potential to become a good probiotic effect regulator.

The invention provides a preparation method of a probiotic microcapsule with high stress resistance, and belongs to the field of foods, pharmaceutical preparations or feeds. The microcapsule prepared by the preparation method has excellent technical effects of excellent high temperature resistance and digestive juice resistance, capability of protecting a core material from being inactivated in a spray drying process, high embedding rate, difficulty in disintegration in simulated gastric acid, good slow-release effect in simulated intestinal juice and the like; and the stability of the probiotics which have heat sensitivity and are not resistant to digestive juice is improved.

According to the high-temperature-resistant and gastric-acid-resistant probiotic soft sweets and the preparation method thereof, a three-layer composite embedding technology (an inner-layer sodium alginate-chitosan gel, a middle-layer whey protein-pectin composite film and an outer-layer HPMCP acid-resistant coating) and a step-by-step temperature control technology are adopted for synergistic effect, so that the high-temperature-resistant and gastric-acid-resistant probiotic soft sweets are prepared; the technical problem that probiotics are easy to inactivate in high-temperature processing and gastric acid environments is solved. Experimental data show that the viable count of the prepared probiotic soft sweets is still kept to be greater than or equal to 7.5 log CFU / g after the probiotic soft sweets are treated at the high temperature of 80 DEG C for 10 minutes, the survival rate of the probiotic soft sweets treated by simulated gastric juice (pH 2.0) for 2 hours is greater than or equal to 95%, the release rate of the probiotic soft sweets in simulated intestinal juice for 30 minutes reaches 92.5%, and the viable retention rate exceeds 87% after the probiotic soft sweets are stored at room temperature for 6 months. By optimizing the probiotic microcapsule structure and the matrix formula, the stability of the probiotics is remarkably improved, and a new technical scheme is provided for the development of the functional soft sweets.

The invention relates to the field of probiotics, in particular to lactobacillus enteri LLM10, a probiotic complexing agent for promoting initial start of replacement gilts and oestrus of primiparous sows and application of the probiotic complexing agent. According to the present invention, the preservation number of the Lactobacillus enteri LLM10 is CGMCC No.26850, and the Lactobacillus enteri LLM10, the cinnamon powder and the Gorgon fruit powder are prepared into the complexing agent; the complexing agent can better enable the lactobacillus enteri LLM0 to be colonized in animal bodies, and has higher tolerance to artificial cholate, artificial gastric acid and artificial intestinal juice; particularly, hormone synthesis and secretion of the replacement gilts and the primiparous sows can be better improved, so that follicular development of the replacement gilts is improved, early starting of the replacement gilts is promoted, timely oestrus of the primiparous sows after weaning is promoted, the oestrus rate is increased, the pregnancy rate is increased, and the number of live piglets is increased.

The tolterodine sustained-release prescription composition comprises a core layer, an isolating layer, a sustained-release layer and an enteric-coated layer, the core layer is a sucrose pellet core, and the surface of the core layer is coated with tolterodine tartrate and hydroxypropyl methylcellulose; the isolation layer is made of hydroxypropyl methylcellulose, and the thickness of the isolation layer is 10-20 microns; the sustained-release layer is prepared from an ethyl cellulose aqueous dispersion and triethyl citrate, and silicon dioxide nanoparticles are added; and the enteric-coated layer is acrylic resin and is dissolved when the pH value is greater than or equal to 5.5. The accumulated dissolution rate of the composition in simulated gastric juice with a pH value of 1.2 within 2 hours is less than or equal to 10%, the accumulated dissolution rate in simulated intestinal juice with a pH value of 6.8 within 7 hours is more than or equal to 85%, and the accumulated dissolution rate in a buffer solution with a pH value of 4.5 within 3 hours is reduced by more than 20% compared with a reference preparation; through collaborative design of multiple layers of coatings, collaborative reinforcement of the plasticizer and the nanoparticles and introduction of the pH response type enteric-coated layer, the acid resistance, the release stability and the targeting property of the medicine are remarkably improved, the problems of burst release of gastric juice and unstable release of intestinal tracts of a traditional preparation are solved, and the preparation has outstanding clinical application value.

The invention discloses a biofilm state lactobacillus culture method for improving the survival rate of lactobacillus, and belongs to the technical field of strain culture. The culture method comprises the following steps: preparing a liquid culture medium; the liquid culture medium contains peptone, beef extract powder, yeast extract powder, glucose, dipotassium phosphate, sodium acetate, magnesium sulfate and manganese sulfate; inoculating lactobacillus into the liquid culture medium, and activating bacterial strains at constant temperature under an anaerobic condition to obtain bacterial suspension; inoculating the bacterial suspension into a culture plate containing the liquid culture medium, and carrying out gradient heating incubation in an anaerobic environment; after incubation is finished, floating bacteria are removed, and biofilm state lactobacillus is obtained through collection. The low-cost raw materials and simple and convenient preparation steps are adopted, the remarkable film forming effect is achieved, the biofilm yield is high, the survival rate of the finally obtained biofilm state lactobacillus in the gastric acid intestinal juice environment can be remarkably increased, and the biofilm state lactobacillus has high practical value.

The invention provides lactobacillus plantarum with high beta-glucosidase activity and application of the lactobacillus plantarum, and belongs to the technical field of microbial fermentation. The lactobacillus plantarum with the high beta-glucosidase activity, provided by the invention, comprises lactobacillus plantarum (Lactobacillus plantarum) LZU-J-LZ1-2 or Lactobacillus plantarum (Lactobacillus plantarum) LZU-J-QA5-2, and the lactobacillus plantarum with the high beta-glucosidase activity, the beta-glucosidase activity, the beta-glucosidase activity, the beta-glucosidase activity and the beta-glucosidase activity, and the beta-glucosidase activity and the beta-glucosidase activity of the beta-glucosidase activity and the beta-glucosidase activity of the beta-glucosidase activity are both high. The lactobacillus plantarum with high beta-glucosidase activity also has acid resistance, salt resistance and gastric juice and intestinal juice tolerance, has strong gastrointestinal tract survival potential, provides precious strain resources for developing efficient and safe multifunctional microbial preparations, and has important industrial application value.

The method for evaluating the vibrio parahaemolyticus VBNC state in the digestive tract in vitro comprises the following steps that simulated gastric juice and vibrio parahaemolyticus bacterial liquid are added into a centrifugal tube, test groups 1, 2 and 3 without glucose are set to be 2.5, 3.5 and 4.5 in pH value and test groups 4, 5 and 6 with the pH value being 2.5, 3.5 and 4.5 and 4 g of glucose are set to be 2.5, 3.5 and 4.5 respectively, after constant-temperature shaking bath culture is carried out for 120 min, simulated intestinal juice is added, then culture continues to be carried out for 120 min, and the vibrio parahaemolyticus VBNC state in the digestive tract is obtained. The simulated digestive juice is extracted at 30 min, 60 min, 120 min and 240 min, a plate counting method, PMAxx-qPCR, flow cytometry, ATP detection, SEM observation and cell membrane permeability detection are conducted on the simulated digestive juice, and the growth and metabolism conditions of the vibrio parahaemolyticus in the digestive tract and the condition that the vibrio parahaemolyticus enters the VBNC state are evaluated; therefore, rapid and systematic evaluation of the vibrio parahaemolyticus in the digestive tract is realized.

The invention provides a microecological preparation for improving animal immunity and a preparation method thereof, and belongs to the field of preparation of polysaccharides and derivatives thereof. The preparation method comprises the following steps: preparing the prebiotic extracting solution, preparing the composite carrier, preparing the composite outer wall and preparing the composite preparation. The loading capacity of the prepared composite carrier is 8.9-9.3%, and the loading capacity of the composite carrier is high; the prebiotic synanthrin component in the prepared microecological preparation is slowly released in the artificial intestinal juice; the synanthrin content of the microecological preparation prepared by the method is 94.5% after the microecological preparation is refrigerated at 5 DEG C for 10 months, the synanthrin content of the microecological preparation is 89.7% after the microecological preparation is stored at 25 DEG C for 10 months, the synanthrin content of the microecological preparation is 80.7% after the microecological preparation is stored at 37 DEG C for 10 months, and the storage capacity of the microecological preparation is high.

This invention relates to a strain of *Lactobacillus plantarum* with uric acid-lowering function (…). Lactobacillus plantarum YLW67 and its applications: This strain was isolated from red sour soup, a traditional fermented food from Guizhou, and is deposited at CGMCC, accession number No. 37373. This strain exhibits excellent tolerance to the gastrointestinal environment, with a survival rate exceeding 40% after treatment in simulated gastric fluid at pH 3.0 and simulated intestinal fluid at pH 8.0. In vitro experiments show that it can completely degrade inosine and guanosine within 9 hours. Animal experiments have confirmed that it can effectively reduce serum uric acid levels in hyperuricemia model mice. This invention also provides a probiotic composition containing this strain and its preparation method, including steps such as large-scale culture, cell collection, freeze-drying, and formulation. This strain and composition can be used to prepare uric acid-lowering functional foods, health foods, or dietary supplements, and have promising applications in improving hyperuricemia and related metabolic diseases.

The present application belongs to the field of microorganisms, and particularly relates to a strain of Enterococcus faecium, a bacterial preparation prepared from the strain, a preparation method of the bacterial preparation and application of the bacterial preparation. The specific technical scheme is as follows: a strain of Enterococcus faecium was preserved in the Guangdong Microbial Culture Collection Center on December 12, 2022, the address of the preservation center is No. 59 Building, 5th Floor, Institute of Guangzhou Martyrs Road, Guangzhou, and the preservation number is GDMCC NO: 62359. The present application provides a new strain of Enterococcus faecium, which is the first discovered Enterococcus faecium with desulfurization and denitrification functions and can be fed. The bacterial preparation prepared by using the Enterococcus faecium has strong resistance to gastric acid and intestinal fluid, can enter the digestive tract through the food channel and survive in large quantities and rapidly colonize in the intestinal tract. After the Enterococcus faecium or the bacterial preparation is ingested by live pigs, the intestinal health degree of the live pigs can be adjusted, the ratio of ammonium nitrogen to total nitrogen, the ratio of sulfur ions to total sulfur and the total content of volatile fatty acids in the intestinal tract are reduced, and the purpose of reducing the emission of foul-smelling gas is achieved from the source.

The invention discloses a lactobacillus acidophilus proliferation agent, a lactobacillus acidophilus preparation and application of the lactobacillus acidophilus proliferation agent. Belongs to the technical field of probiotics. The lactobacillus acidophilus proliferation agent is dendrobium officinale ultra-fine powder, preferably, Yunnan dendrobium officinale ultra-fine powder, and the particle size of the lactobacillus acidophilus proliferation agent is smaller than or equal to 10 microns. According to the proliferation agent, the growth rate and the thallus number of the lactobacillus acidophilus in the culture process can be increased, the resistance of the lactobacillus acidophilus to cholate, gastric juice and intestinal juice is enhanced, and the survival rate of the lactobacillus acidophilus in gastrointestinal tracts is increased. The invention also provides a thallus preparation, which comprises lactobacillus acidophilus viable thallus and dendrobium officinale ultra-fine powder. The invention also relates to application of the product in preparation of food or drugs for improving intestinal microecology, and a method for promoting growth of lactobacillus acidophilus and improving gastrointestinal tract tolerance of lactobacillus acidophilus.

The invention discloses gel microspheres co-loaded with iron and vitamin D as well as a preparation method and application of the gel microspheres, and belongs to the technical field of nutrient enrichment compounds. The preparation method disclosed by the invention comprises the following steps: swelling a whey protein isolate fiber solution and an artemisia desertorum polysaccharide solution, and mixing to obtain a mixed solution; then adding algal oil into the mixed solution, and emulsifying the obtained mixture to obtain O / W type composite emulsion; adding the O / W type composite emulsion into a FeSO4 solution A, mixing, and carrying out static oxidation to obtain composite emulsion gel; and extruding the composite emulsion gel into a FeSO4 solution B, and carrying out cross-linking curing to obtain the gel microspheres co-loaded with iron and vitamin D. According to the method disclosed by the invention, the obtained microspheres can keep a stable structure in a simulated gastric juice environment and realize gradient release of iron ions and vitamin D in simulated intestinal juice, and the oral bioavailability of the two nutrients is remarkably improved.

The invention relates to the technical field of microorganisms, and particularly provides lactobacillus plantarum as well as a derivative, a composition and application thereof. The lactobacillus plantarum disclosed by the invention is preserved in the China Center for Type Culture Collection, the preservation number of the lactobacillus plantarum is CCTCC NO: M20252083, the lactobacillus plantarum is named as Lactobacillus plantarum (Lactobacillus plantarum)-4800m, and the lactobacillus plantarum has the advantages of strong gastric juice and intestinal juice tolerance, strong oxidation resistance, good intestinal adhesion and colonization potential and wide application prospect.

The invention discloses a method for preparing a composition for treating ulcerative colitis, and relates to a method for preparing a composition for treating ulcerative colitis. The present invention is to solve the problem that the existing method for treating ulcerative colitis is difficult to reach the lesion site intact. Method: one, carbonization of a mixture of sanguisorba officinalis and black plum; two, extraction and purification of the carbonized product; three, preparation of palm charcoal-purslane; four, preparation of gallnut and pomegranate peel fermented extract; five, preparation of gel micropills; six, preparation of gallnut and pomegranate peel fermented product colon-localized micropills; mixing the gel micropills with gallnut and pomegranate peel fermented product colon-localized micropills, to obtain. In an in vitro release test, the double colon micropills prepared in the present application can basically not release in an environment of simulated gastric acid for 2h, release less in an environment of simulated small intestinal fluid for 3h, only about 6%, and can release more than 90% in an environment of simulated colon fluid for 7h. The present invention belongs to the field of preparation of drugs for treating ulcerative colitis.

This invention belongs to the field of microbial technology, specifically relating to a strain of *Pediococcus acidilactici* SUN01 with acetylcholinesterase inhibitory activity and its applications. This *Pediococcus acidilactici* SUN01 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 29704. The *Pediococcus acidilactici* SUN01 provided by this invention exhibits strong acid and bile salt resistance, good tolerance in simulated gastric and intestinal fluids, and can successfully reach the human intestine. *Pediococcus acidilactici* SUN01, in its bacterial suspension and inactivated cells, possesses strong acetylcholinesterase inhibitory activity, produces γ-aminobutyric acid (GABA), and has antioxidant and other probiotic functions. Its application in probiotic products may help improve Alzheimer's disease, enhance learning and memory abilities, regulate mood, and improve sleep.

The invention relates to a plant lactobacillus strain LES-JC-L-07, which is characterized in that the preservation number of the strain is CGMCC No.35733. The invention provides a selenium-rich plant lactobacillus which is separated from Dali special food dairy fan and is successfully preserved, the provided plant lactobacillus has a selenium-rich rate up to 85-91%, the selenium-rich rate is increased to 93-100% after selenium-rich condition optimization, and the selenium-rich rate is increased to 93-100% after selenium-rich condition optimization. The plant lactobacillus provided by the invention has strong high-concentration sodium selenite resistance, strong acid resistance, strong cholate resistance and relatively good tolerance to gastric juice and intestinal juice, can efficiently convert and utilize sodium selenite, can be used for producing and preparing organic selenium, and can be used for preparing organic selenium. Such as probiotic solid beverage preparation, fermented food production, milk processing and the like.