Lactobacillus reuteri SXDT-32 and application thereof

A technology of SXDT-32 and Lactobacillus reuteri, which is applied in the field of probiotics, can solve problems such as the incidence of piglet diarrhea and increased mortality, so as to reduce animal diarrhea and mortality, reduce diarrhea rate, and improve intestinal immunity Effect

Active Publication Date: 2022-08-02
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ban on antibiotics led to a sharp increas

Method used

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  • Lactobacillus reuteri SXDT-32 and application thereof
  • Lactobacillus reuteri SXDT-32 and application thereof
  • Lactobacillus reuteri SXDT-32 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Lactobacillus reuteri ( Lactobacillus reuteri ) isolation and 16S rRNA identification

[0039] Isolation and identification of Lactobacillus reuteri: Lactobacillus reuteri ( Lactobacillus reuteri ) was isolated from horse body pig manure in the northern four seasons pasture in the ancient town of Yanggao County, Datong City, Shanxi Province.

[0040] 1. Feces collection: Collect 1 g of feces from 20 fattening horse body pigs, add 20 ml of sterile saline containing 20% ​​glycerol, vortex and mix, and distribute them into sterile cryopreservation tubes, each tube of 1 ml. Store in dry ice after processing, bring back to the laboratory and store at -80°C until use.

[0041] 2. Bacterial isolation: Take 1 ml of horse body pig feces, carry out 10-fold gradient dilution with sterile saline, draw 0.1 mL of the appropriate dilution and spread it evenly on MRS agar, and cultivate it at 37 ° C for 48 hours. The obtained colonies were inoculated into new MRS solid med...

Embodiment 2

[0044] Example 2 Lactobacillus reuteri ( Lactobacillus reuteri ) feature detection

[0045] 1. Antibacterial ability test

[0046] The pathogenic indicator bacteria were selected from two common pathogenic bacteria: Escherichia coli ( Escherichia coli ) BW25113 and Salmonella typhimurium ( Salmonella typhimurium ) ATCC14028.

[0047] The antibacterial activity of the strains was determined by the Oxford cup method. Escherichia coli BW25113 and Salmonella typhimurium ( Salmonella typhimurium ) ATCC14028 were respectively inoculated in LB broth medium, placed in a 37°C incubator for 12-18h. Escherichia coli ( Escherichia coli ) BW25113 and Salmonella typhimurium ( Salmonella typhimurium ) The concentration of ATCC14028 strain was adjusted to 1×10 with sterile saline 8 Take 200 μl of CFU / mL each, spread them evenly on LB agar medium, put the sterilized Oxford cup evenly on the LB medium, and add 200 μL of activated bacteria to the Oxford cup and the concentration of ...

Embodiment 3

[0072] Example 3 Effect of Lactobacillus reuteri SXDT-32 on intestinal health of DSS model mice

[0073] Through the above experiments, it was determined that Lactobacillus reuteri ( Lactobacillus reuteri ) SXDT-32 strain has the best anti-stress and probiotic properties, and is preserved in the General Microbiology Center of the China Microorganism Culture Collection Management Committee, with the preservation number of CGMCC No. 24727. Therefore, Lactobacillus reuteri ( Lactobacillus reuteri ) SXDT-32 strain was used for post-go experiments.

[0074] 1. Experimental method

[0075] 1. 36 20-day-old ICR male mice were selected and raised in the Animal Center of the Institute of Animal Science, Chinese Academy of Agricultural Sciences. During the experiment, the temperature of the mouse room was controlled at 21±2°C, the light / dark time was 12 hours, and food and water were freely taken.

[0076] 2. The mouse colon inflammation injury model was established with dextran sod...

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Abstract

The invention relates to the field of probiotics, in particular to lactobacillus reuteri SXDT-32 and application thereof. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection The strain has a good inhibition effect on escherichia coli and salmonella; the strain can be colonized in an animal body, and has strong tolerance to artificial cholate, artificial gastric acid and artificial intestinal juice; the feed additive can be firmly adhered and colonized in animal intestinal tracts, inhibits growth of pathogenic microorganisms in the intestinal tracts, improves animal intestinal tract immunity, relieves animal colitis, reduces animal diarrhea and death rate, improves animal intestinal tract health, and also can improve animal growth performance.

Description

technical field [0001] The invention relates to the field of probiotics, in particular to Lactobacillus reuteri SXDT-32 and applications thereof. Background technique [0002] The stress caused by early weaning is mainly manifested as piglet diarrhea, resulting in reduced piglet growth performance and increased mortality. Although antibiotics have significantly improved the efficacy of diarrheal treatment over the past few decades, the development and spread of drug-resistant strains, often resulting in antibiotic residues in food, has become a serious problem. As a result, many countries are phasing out the use of antibiotics in animal production. However, the prohibition of antibiotics led to a sharp increase in the incidence of diarrhea and mortality in piglets. Therefore, there is an urgent need to develop green, safe, efficient and non-toxic treatment methods for diarrhea. [0003] At present, research on probiotics has become a hot spot. Probiotics have been found ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A61K35/747A61P31/04A61P1/00A61P29/00A61P37/04A61P1/12C12R1/225
CPCC12N1/205A23K10/18A61K35/747A61P31/04A61P1/00A61P29/00A61P37/04A61P1/12C12R2001/225A23V2400/173Y02A50/30
Inventor 陈亮骆城增张宏福
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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