Application of fucoidin and hydrolyzed oligosaccharide thereof in preparing probiotic protection agent and method

A technology of fucoidan and probiotics, which is applied in the direction of being fixed on/in an organic carrier to achieve the effects of improving survival rate, improving biological efficacy and good stress resistance

Active Publication Date: 2019-08-09
SOUTHWEST UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether fucoidan and its hydrolyzed oligosaccharide-encap

Method used

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  • Application of fucoidin and hydrolyzed oligosaccharide thereof in preparing probiotic protection agent and method
  • Application of fucoidin and hydrolyzed oligosaccharide thereof in preparing probiotic protection agent and method
  • Application of fucoidin and hydrolyzed oligosaccharide thereof in preparing probiotic protection agent and method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0043] Example 1. The effect of embedding oligosaccharide 1 on the growth of Bacillus subtilis

[0044] Probiotic treatment: Centrifuge the Bacillus subtilis fermentation broth at 4°C and 4000 × g for 10 min, wash twice with PBS, and suspend the pelleted cells in the same PBS (pH 7.0), then store at 4°C. spare.

[0045] Unembedded group: 2 ml of concentrated Bacillus subtilis solution and 10 ml of NaCl solution with pH=7 were rotated for 30 min to form a monolayer embedded group (simple mixing).

[0046] Fucoidan embedding preparation: 2mL concentrated Bacillus subtilis solution (20~70*10 7 CFU) was mixed with 10 mL of fucoidan with a concentration of 20 g / L, and rotated for 30 min to prepare a monolayer embedding group (simple mixing).

[0047] Preparation of oligosaccharide 1 embedding: Mix 2 mL of concentrated Bacillus subtilis solution with 10 mL, 20 g / L of oligosaccharide 1, and rotate for 30 min to prepare a monolayer embedding group (simple mixing).

[0048]The unemb...

Example Embodiment

[0053] Embodiment 2, the effect of embedding oligosaccharide 2 on the growth of Bacillus subtilis

[0054] Probiotic treatment: Bacillus subtilis fermentation broth was centrifuged at 4°C, 4000×g for 10 min, washed twice with PBS, and the precipitated cells were evenly suspended in the same PBS (pH 7.0), and then stored at 4°C for later use.

[0055] Unembedded group: 2ml of concentrated Bacillus subtilis solution and 10ml of pH7NaCl solution were rotated for 30min to form monolayer embedded group (simple mixing).

[0056] Preparation of Fucoidan Embedding: Mix 2ml of concentrated Bacillus subtilis solution with 10mL of 40g / L fucoidan, and rotate for 30min to prepare a monolayer embedding group (simple mixing).

[0057] Preparation of oligosaccharide 2 embedding: Mix 2ml of concentrated Bacillus subtilis solution with 10mL of 40g / L oligosaccharide 1, and rotate for 30min to prepare a monolayer embedding group (simple mixing).

[0058] The unembedded group, the fucoidan-embedd...

Example Embodiment

[0063] Example 3. Effect of monolayer-embedded oligosaccharide 1 on the growth of L. marxianus

[0064] Probiotic treatment: L. marxianus fermentation broth was centrifuged at 4°C, 4000 × g for 10 min, washed twice with PBS, and the precipitated cells were evenly suspended in the same PBS (pH 7.0), and stored at 4°C for later use.

[0065] Unembedded group: 2 ml of concentrated L. marxianus solution and 10 ml of pH7NaCl solution were rotated for 30 min to form a monolayer embedded group (simple mixing).

[0066] Preparation of Fucoidan Embedding: Mix 2ml of concentrated L. marxianus with 10mL of 40g / L fucoidan, and rotate for 30min to prepare a monolayer embedding group (simple mixing).

[0067] Preparation of oligosaccharide 1 embedding: Mix 2 ml of concentrated L. marxianus bacterial solution with 10 mL of 40 g / L oligosaccharide 1, and rotate for 30 min to prepare a monolayer embedding group (simple mixing).

[0068] The unembedded group, the fucoidan-embedded group and the...

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Abstract

The invention discloses application of fucoidin and hydrolyzed oligosaccharide thereof in preparing a probiotic protection agent and a method. According to the application and the method, the fucoidinand the hydrolyzed oligosaccharide thereof embed probiotics for the first time, the survival conditions in gastric acid and small intestinal fluid are simulated, it is shown through a result that thesurvival time of the probiotics embedded by the fucoidin and the hydrolyzed oligosaccharide is prolonged, the embedding effect of the hydrolyzed oligosaccharide is better than that of the fucoidin, and a foundation is laid for prolonging the high activity of the probiotics in the gastrointestinal environment.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a method for embedding and protecting probiotics by using fucoidan produced by fermentation and its hydrolyzed oligosaccharide. Background technique [0002] Fucoidan is a polysaccharide containing a rare sugar fucose structure, and its main sources include plant extraction and microbial fermentation. The plant extraction method is mainly extracted from brown algae and some marine invertebrates. It has a unique water-soluble polysaccharide combined with sulfate groups, also known as fucoidan sulfate fucoidan; microbial fermentation method is mainly through some Enterobacter species Preparation by fermentation. Existing studies have shown that fucoidan has two-way regulation of immunity, scavenging free radicals, anti-aging, anti-coagulation and anti-thrombosis, anti-tumor and HIV virus, eliminating gastrointestinal system disorders, anti-allergy, enhancing liver function, and reducing...

Claims

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Application Information

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IPC IPC(8): C12N11/10
CPCC12N11/10
Inventor 邹祥谌颉刘超李姗姗夏海钦王振宇
Owner SOUTHWEST UNIVERSITY
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