Application of fucoidin and hydrolyzed oligosaccharide thereof in preparing probiotic protection agent and method
A technology of fucoidan and probiotics, which is applied in the direction of being fixed on/in an organic carrier to achieve the effects of improving survival rate, improving biological efficacy and good stress resistance
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[0043] Example 1. The effect of embedding oligosaccharide 1 on the growth of Bacillus subtilis
[0044] Probiotic treatment: Centrifuge the Bacillus subtilis fermentation broth at 4°C and 4000 × g for 10 min, wash twice with PBS, and suspend the pelleted cells in the same PBS (pH 7.0), then store at 4°C. spare.
[0045] Unembedded group: 2 ml of concentrated Bacillus subtilis solution and 10 ml of NaCl solution with pH=7 were rotated for 30 min to form a monolayer embedded group (simple mixing).
[0046] Fucoidan embedding preparation: 2mL concentrated Bacillus subtilis solution (20~70*10 7 CFU) was mixed with 10 mL of fucoidan with a concentration of 20 g / L, and rotated for 30 min to prepare a monolayer embedding group (simple mixing).
[0047] Preparation of oligosaccharide 1 embedding: Mix 2 mL of concentrated Bacillus subtilis solution with 10 mL, 20 g / L of oligosaccharide 1, and rotate for 30 min to prepare a monolayer embedding group (simple mixing).
[0048]The unemb...
Example Embodiment
[0053] Embodiment 2, the effect of embedding oligosaccharide 2 on the growth of Bacillus subtilis
[0054] Probiotic treatment: Bacillus subtilis fermentation broth was centrifuged at 4°C, 4000×g for 10 min, washed twice with PBS, and the precipitated cells were evenly suspended in the same PBS (pH 7.0), and then stored at 4°C for later use.
[0055] Unembedded group: 2ml of concentrated Bacillus subtilis solution and 10ml of pH7NaCl solution were rotated for 30min to form monolayer embedded group (simple mixing).
[0056] Preparation of Fucoidan Embedding: Mix 2ml of concentrated Bacillus subtilis solution with 10mL of 40g / L fucoidan, and rotate for 30min to prepare a monolayer embedding group (simple mixing).
[0057] Preparation of oligosaccharide 2 embedding: Mix 2ml of concentrated Bacillus subtilis solution with 10mL of 40g / L oligosaccharide 1, and rotate for 30min to prepare a monolayer embedding group (simple mixing).
[0058] The unembedded group, the fucoidan-embedd...
Example Embodiment
[0063] Example 3. Effect of monolayer-embedded oligosaccharide 1 on the growth of L. marxianus
[0064] Probiotic treatment: L. marxianus fermentation broth was centrifuged at 4°C, 4000 × g for 10 min, washed twice with PBS, and the precipitated cells were evenly suspended in the same PBS (pH 7.0), and stored at 4°C for later use.
[0065] Unembedded group: 2 ml of concentrated L. marxianus solution and 10 ml of pH7NaCl solution were rotated for 30 min to form a monolayer embedded group (simple mixing).
[0066] Preparation of Fucoidan Embedding: Mix 2ml of concentrated L. marxianus with 10mL of 40g / L fucoidan, and rotate for 30min to prepare a monolayer embedding group (simple mixing).
[0067] Preparation of oligosaccharide 1 embedding: Mix 2 ml of concentrated L. marxianus bacterial solution with 10 mL of 40 g / L oligosaccharide 1, and rotate for 30 min to prepare a monolayer embedding group (simple mixing).
[0068] The unembedded group, the fucoidan-embedded group and the...
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