Lactobacillus capable of adsorbing or degrading enterotoxin and application thereof

A technology of enterotoxin and lactic acid bacteria, applied in the field of lactic acid bacteria, can solve the problems of long effective time, dependence, side effects, etc., achieve good acid resistance and bile salt resistance, and enhance the effect of immunity

Active Publication Date: 2015-10-21
山东凤凰生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, detoxification and beauty care products can be divided into four categories according to their characteristics and main functional ingredients: the first category uses rhubarb and other laxatives as the main functional ingredients, which have side effects such as abdominal pain and diarrhea. Long-term use can lead to intestinal dysfunction, such as detoxification and beauty capsule
The second category uses cassia seed, yam and aloe as the main functiona

Method used

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  • Lactobacillus capable of adsorbing or degrading enterotoxin and application thereof
  • Lactobacillus capable of adsorbing or degrading enterotoxin and application thereof
  • Lactobacillus capable of adsorbing or degrading enterotoxin and application thereof

Examples

Experimental program
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Example Embodiment

[0025] Example 1 Screening of lactic acid bacteria with the function of removing enterotoxin

[0026] 1.1 The cultivation of lactic acid bacteria and the preparation of fermentation broth

[0027] Lactic acid bacteria were cultured in MRS liquid medium at 37°C for 24h, 6000r / min, and centrifuged at 4°C for 10min. The supernatant was collected as the fermentation broth.

[0028] 1.2 Determination of the tolerance of lactic acid bacteria in different toxins and different concentrations

[0029] Draw 2mL of mother liquor of indole, phenol, and p-cresol and 100mL of MRS medium to prepare medium with toxin concentration of 0.1, 0.1, and 0.5mg / mL respectively, and inoculate lactic acid bacteria 24h fermentation broth (2%) for 12h, 24h, The live bacteria were sampled for 32 hours to count, and the toxin-free culture broth was used as a control to calculate the survival rate of lactic acid bacteria. Mother liquor preparation: phenol (the temperature of 60°C turns into liquid from crystalline...

Example Embodiment

[0032] Example 2 Acid resistance test

[0033] Use HCl to adjust the pH value of physiological saline to 1.5, 2.0, 3.0, 115 ℃, sterilize for 30 min, aseptically access the fermentation broth for 24 h according to 1% inoculation amount, and at 37 ℃ for 1 h, 2 h, Sampling at 3h to determine the number of viable bacteria, and saline as a control. Survival rate (%) = the number of viable bacteria in the bacterial solution to be tested / the number of viable bacteria in the salt water×100 Example 3 Bile salt tolerance test

Example Embodiment

[0049] Table 3 Test results of tolerance of lactic acid bacteria to different concentrations of phenol

[0034] The bacterial solution was inoculated into MRS medium with bile salt concentration of 0.1%, 0.2%, 0.3%, (w / v) at 1% (v / v). MRS medium without bile salt was used as the control. After culturing for 0h, 2h, 4h, and 6h at ℃, samples were taken to count the plate colonies to calculate the survival rate of Lactobacillus.

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Abstract

The invention discloses a lactobacillus capable of adsorbing or degrading enterotoxin and an application thereof. The preservation number of the lactobacillus is CCTCC No: M2015128. The lactobacillus can adsorb or degrade enterotoxin and has the good effects of resisting acid, cholate, artificial gastric juice and artificial intestinal juice. The lactobacillus is specifically characterized in that the enterotoxin can be adsorbed or degraded, and good enterotoxin removal capacity is achieved; growth of common pathogenic bacteria can be restrained; the lactobacillus can resist acid and cholate and can survive in the digestive tract. In specific application, a method includes the step that freeze-dried bacterial powder is dissolved in water at normal temperature to be taken orally or is directly added according to a certain proportion to make functional food or healthcare products. The lactobacillus is screened from multiple lactobacilli, has the high enterotoxin removal capacity and good performance, and can be used for human body healthcare.

Description

technical field [0001] The invention relates to a lactic acid bacterium with the function of clearing intestinal toxins and its application, which can be applied to functional food and health food, and belongs to the field of biotechnology. Background technique [0002] A toxin is a substance that can interfere with normal physiological activities and disrupt body functions. Pollution from the external environment, unhealthy diet and other factors lead to the production of toxins in the body. Toxins in the human body include free radicals, stool, cholesterol, uric acid, lactic acid, water poisoning and congestion, etc., and endogenous toxins are produced during the metabolic process, such as toxins produced by sugar, fat, and protein metabolic disorders, and toxins produced by the accumulation of stool in the intestine. [0003] The toxins stored in the human body not only disrupt the normal digestion and absorption of the human body, but also circulate to various organs of...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/29A61K35/747A61P39/02A61P37/04C12R1/25
Inventor 单宝龙王静任宝涛张颜廷杨婷婷庄金丽
Owner 山东凤凰生物科技股份有限公司
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