A strain of Lactobacillus plantarum with antibacterial properties and its application in the prevention of diarrhea

A technology of Lactobacillus plantarum and characteristics, applied in the biological field, can solve the problems of serious strain homogeneity and lack of functional subdivision products, etc., and achieve good adhesion characteristics, reduce adhesion rate, and excellent antibacterial effect

Active Publication Date: 2022-01-18
HANGZHOU WAHAHA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the domestic probiotics market has developed rapidly, but the product form is still dominated by fermented dairy products such as yogurt and lactic acid bacteria beverages, and the function claim is still mainly to regulate the function of the gastrointestinal tract and balance the intestinal flora, lacking further functional segmentation product
At the same time, the strains used in the domestic probiotics market basically rely on the monopoly supply of foreign companies such as DuPont and Chr.

Method used

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  • A strain of Lactobacillus plantarum with antibacterial properties and its application in the prevention of diarrhea
  • A strain of Lactobacillus plantarum with antibacterial properties and its application in the prevention of diarrhea
  • A strain of Lactobacillus plantarum with antibacterial properties and its application in the prevention of diarrhea

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Antibacterial properties of Lactobacillus plantarum 589

[0032]The indicator bacteria were Micrococcus luteus, Escherchia coli CICC 23689, Staphylococcus aureus CICC 10301, Salmonella typhimurium CICC 10437, Shigella flexneri CICC 21534 ) four common intestinal infection pathogens. Using NB medium, under the condition of 37 ℃ culture, after three generations of continuous activation, it was used for antibacterial experiment. The antibacterial properties of the bacterial strains of the present invention were measured by a double-layer plate punching diffusion method. Pour the sterilized 1.5% agar into a sterile petri dish. After solidification, cool down the sterilized solid medium (containing 1.1% agar) to a certain temperature, mix it with the indicator bacteria and pour it into Placed on top of the agar layer in an Oxford cup. The indicator bacteria should be adjusted to a reasonable concentration according to the growth conditions. After the upper layer is solid...

Embodiment 2

[0041] Tolerance Characteristics of Lactobacillus plantarum 589

[0042] After culturing the strains of the present invention and commercially available commercial strains to the end of logarithmic growth, the bacterial suspension was obtained, and the bacterial suspension was divided into two groups, and the acid resistance and bile salt resistance were measured respectively. The operation is as follows. (1) Take a certain amount of bacterial liquid, centrifuge at 4000g / 10min and discard the supernatant. Add the same volume of MRS solution with pH=2.5, pipette and mix well, incubate at 37°C, and measure the changes in the number of bacteria at 0 point and after incubation for 1h, 2h, and 3h by the dilution smear counting method. (2) Take a certain amount of bacterial liquid, 4000g, centrifuge for 10min and discard the supernatant. Add the same volume of MRS solution containing 0.3% bile salts, pipette and mix well, incubate at 37°C, and measure the changes in the number of b...

Embodiment 3

[0046] Intestinal adhesion ability of Lactobacillus plantarum 589

[0047] strain Adhesion rate (number of bacteria / number of cells) Lactobacillus plantarum 589 1.96±0.15 control bacteria 1.75±0.30

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Abstract

The invention provides a new strain of Lactobacillus plantarum with the function of preventing diarrhea and protecting the intestinal tract. The bacterial strain can inhibit the growth of common intestinal pathogenic bacteria and intestinal colonization, and the bacterial strain has the function of gastric acid and bile salts. With good tolerance, it can reach the intestinal tract smoothly and adhere to the intestinal epithelial cells to exert its intestinal protection function. In addition to inhibiting the production of intestinal pathogenic bacteria, the strain of the present invention can better exert the function of guarding the intestinal tract and preventing diarrhea through the above characteristics.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a new strain of Lactobacillus plantarum and the application of the strain in preventing diarrhea and protecting intestinal tract. Background technique [0002] Diarrhea is a common disease in people's daily life, showing seasonal and geographical differences. Summer and autumn are seasons with a high incidence of diarrhea. In addition to factors such as fatigue and cold, bacterial infection is an important cause of diarrhea in summer and autumn. In the hot and humid environment in summer and autumn, pathogenic microorganisms multiply quickly, and food is easy to deteriorate. If you do not pay attention to food hygiene, eating food contaminated by Escherichia coli, Shigella, Salmonella, Campylobacter, Vibrio, etc. cause diarrhea symptoms of varying degrees. On the other hand, with the improvement of people's living standards, traveling even abroad has gradually become a part of peop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/742A61P1/12A61P1/00A23L29/00A23L33/135A23L2/38A23L2/52A23K10/12A23K10/18C12R1/25
CPCA61P1/00A61P1/12A23K10/12A23K10/18A23L2/382A23L2/52A23L29/065A23L33/135C12N1/20A23V2002/00A61K2035/115C12R2001/25C12N1/205A23V2400/169A23V2200/3204
Inventor 陈作国朱珺李言郡孙盛陈苏
Owner HANGZHOU WAHAHA TECH
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