37results about How to "Good acid and bile salt resistance" patented technology

The invention discloses lactobacillus plantarum N3117. The lactobacillus plantarum N3117 is acid-resistant and bile-salt-resistant probiotic separated from a fermented dairy product in Inner Mongolia; the strain of the lactobacillus plantarum N3117 is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.10133; the screened lactobacillus plantarum N3117 is sequentially subjected to tests including sample enrichment, separation, purification and the like, and the lactobacillus plantarum N3117 is screened out finally. The screened lactobacillus plantarum N3117 can be used for preparing yoghurt, sour milk beverages and probiotic powder preparations containing the lactobacillus plantarum N3117. The method is applicable to screening of the lactobacillus plantarum N3117 and the lactobacillus plantarum N3117 is applicable to preparation of functional dairy products and probiotic powder containing the lactobacillus plantarum N3117.

The invention discloses lactobacillus with high antioxidant activity. The lactobacillus named as lactobacillus plantarum BLCC2-0015 is preserved in China Center for Type Culture Collection on March 18, 2015 with the preservation number of CCTCC NO.M2015126. The lactobacillus with high antioxidant activity has the advantages that the lactobacillus is screened from numerous lactobacillus, the lactobacillus is high in antioxidant activity and good in acid and bile salt tolerance, the lactobacillus powder made by the lactobacillus can be produced into body antioxidant food or health care products which can be directly eaten or mixed with water for drinking, use convenience is achieved, and body immunity can be enhanced.

The invention discloses a lactobacillus kefiri KL22 as well as a screening method and application thereof. The strain is collected in China General Microbiological Culture Collection Center (CGMCC) on September 16, 2014 and has a collection number of CGMCC No. 9669. Screened kefiri grains are sequentially subjected to separation, purification, a sugar fermentation characteristic experiment, a molecular biology identification, acid and bile salt tolerance characteristic experiment, an intestinal cell adhesion characteristic experiment, a harmful bacteria inhibition experiment and a strain fermentation characteristic experiment to finally screen lactobacillus kefiri KL22. The screened KL22 can be used for preparing yogurt, lactic bacteria drinks and bacterial powder preparations containing lactobacillus kefir KL22. The method is suitable for screening the lactobacillus kefiri KL22 which is used for preparing functional dairy products and probiotics powder containing lactobacillus kefiri KL22.

The invention relates to the field of microorganisms and discloses lactobacillus plantarum with a weight reducing function and application thereof. The lactobacillus plantarum 1701 strain is separatedfrom a yogurt powder sample collected from rural areas of Shigatse in the Tibet Autonomous Region. The strain is preserved in the China General Microbiological Culture Collection Center on October 23, 2019, and the microorganism has the preservation number of CGMCC NO.18728. The lactobacillus plantarum 1701 has excellent tolerance and adhesion, can remarkably reduce the weight of a rat, reduce fat accumulation in a body, reduce the viscera ratio and body fat ratio, reduce the leptin level and reduce blood lipid and can be used in medicines, health-protecting products and health foods for preventing and treating obesity. Furthermore, the strain still has a weight reducing function after inactivation, thus having rich product mode, wide application range, high product stability and long preservation period.

The invention relates to the field of probiotics, in particular to a preparation method of probiotic granules being resistance to acid and bile salt. The preparation method comprises the following steps of S1, uniformly mixing probiotic bacterial sludge with a protective agent, and performing slow stirring to obtain an emulsion; S2, adding prepared colloid liquid to the obtained emulsion, uniformly mixing and stirring the obtained emulsion with the colloid liquid, and performing slow stirring to obtain mixing liquid; S3, slowly injecting the obtained mixing liquid into a calcium chloride solution, performing a crosslinking reaction, after the mixing liquid is completely injected, performing high-speed shearing and stirring, and then performing standing to obtain mixed bacteria starch; andS4, performing freeze drying on the obtained mixed bacteria starch to obtain solids of which the water content is lower than 5%, and grinding and granulating the obtained solids by a grinding and granulating machine to obtain 20-50-mesh probiotic granules, wherein the protective agent comprises trehalose, glycerine and modified starch, and the colloid liquid comprises sodium alginate. The probiotic granules obtained through the preparation method disclosed by the invention have favorable acid resistance and bile salt resistance, and when the probiotic granules achieve intestinal tracts throughstomachs, the activity can be maintained to a great extent.

The invention relates to the field of microorganisms, and discloses lactobacillus fermentum capable of reducing blood uric acid. The strain is named 2644 strain, and is preserved at the China GeneralMicrobiological Culture Collection Center on 19th, November, 2018, and a microbial preservation number is CGMCC NO.16754; and the preservation authority address is NO.3 1# Courtyard, Beichen West Road, Chaoyang District, Beijing, and the microorganism is classified and named Lactobacillus fermentum. The strain can effectively reduce the content of the blood uric acid; and not only can intake of exogenous purines be reduced, but also discharge of internal uric acid from an intestinal tract can be promoted so that the blood uric acid level of a body can be more effectively controlled.

The invention discloses probiotic powder with an anti-oxidation function and a preparation method thereof. The probiotic powder with the anti-oxidation function is prepared from lactobacillus kefir KL22 and lactobacillus plantarum N3117; and oligosaccharides, marine polysaccharides and maltodextrin are utilized as compound components so as to carry out processing and preparation. The prepared probiotic powder has very good hydroxyl free radical (HO.) scavenging capability, superoxide anion free radical (O2-.) scavenging capability and 1,1-diphenyl-picryl hydrazinyl free radical scavenging capability.

The invention discloses a lactobacillus plantarum novel strain with the function of diarrhea prevention and intestinal tract protection. The strain has the restraining effect on common pathogenic entero becterium growth and intestinal tract planting, the strain has the good tolerance for gastric acid and chelate, can smoothly reach the intestinal tract and can be attached to intestinal tract epithelial cells, and the intestinal tract protection function is brought into play. The strain has the restraining effect on generation of pathogenic entero becteria, and the strain can better achieve theintestinal tract protection and diarrhea prevention functions due to the characteristics.

The invention provides a preparation method of a traditional Chinese medicine microecological preparation for preventing and treating livestock diarrhea. The preparation method comprises the step of inoculating a seed solution into a culture medium for fermentation; wherein the culture medium contains a compound traditional Chinese medicine extracting solution and a matrix culture medium, and theseed solution contains traditional Chinese medicine fermentation strains. The invention further provides the traditional Chinese medicine microecological preparation for preventing and treating livestock diarrhea and bacillus coagulans CCTCC NO: M2019722. The traditional Chinese medicine microecological preparation is mainly used for preventing and treating livestock diarrhea, has the exact curative effect, greatly reduces the use amount of traditional Chinese medicines through microbial fermentation, saves the management cost of farmers, and has good prevention and treatment effects.

The invention relates to the field of foods, and discloses a composition based on lactobacillus plantarum and a preparation method of the composition. The composition comprises lactobacillus plantarumfreeze-dried powder, white kidney bean extract, inulin, erythritol and vitamin C. The composition is capable of reducing body weight, reducing fat accumulation in the body, reducing the organ ratio and the body fat ratio, reducing the leptin level and reducing blood fat, and can be used for preventing and treatment obesity. The lactobacillus plantarum 1701 used for preparing the lactobacillus plantarum freeze-dried powder has good tolerance and adhesiveness, can significantly reduce body weight, reduce fat accumulation in the body, reduce the organ ratio and the body fat ratio and reduce theleptin level and blood fat, and can be used for preventing and treatment obesity; and in addition, after being inactivated, the lactobacillus plantarum 1701 also has the function of losing weight, sothat the stability and the shelf life of a product are longer.

The invention belongs to the technical field of poultry and livestock culture feed additives, and provides a bacillus subtilis preparation capable of being applicable to intestinal tract fixed implantation and improving digestion rate and immunity. The bacillus subtilis preparation can be fixedly implemented into the animal intestinal tract to improve the microbial flora balance in the host intestinal tract and to achieve the antibacterial and disease prevention effects, can also generate various amylase, cellulase and protease in the animal intestinal tract to improve the animal feed utilization rate. The bacillus subtilis mud and an adsorption agent are mixed according to the mass ratio of 1:9; spray drying is performed to obtain bacterial powder; the adsorption agent uses the total weight of the adsorption agent as the calculation reference, and is prepared by proportionally mixing soluble starch, jerusalem artichoke powder, saccharose and galactose. The feed utilization rate can beimproved; the antibacterial effects are achieved on the intestinal tract pathogenic bacteria; high acid resistance and bile salt tolerance capability are realized; the stable fixed implementation isrealized in the intestinal tract; a novel breeding technology is provided for poultry and livestock industry. The referential breeding technology is provided for other plant varieties difficult to perform normal sexual propagation.

The present invention relates to lactobacillus plantarum. The name of the lactobacillus plantarum is lactobacillus plantarum 021, the classification name of the lactobacillus plantarum is lactobacillus plantarum, the preservation number of the lactobacillus plantarum is CGMCC No.22169, the preservation date of the lactobacillus plantarum is April 12, 2021, and the preservation unit of the lactobacillus plantarum is China General Microbiological Culture Collection Center, Institute of Biology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing. The lactobacillus plantarum disclosed by the invention has better acid-resistant, cholate-resistant, acid-producing and hydrogen peroxide-producing capabilities. The lactobacillus plantarum has a good adhesion effect on cervical epithelial cells, and meanwhile has a good capability of inhibiting candida albicans and staphylococcus aureus and high antibiotic anti-sensitivity. In addition, the lactobacillus plantarum has an inhibiting effect on candida albicans infectious vaginosis.

The invention relates to the field of foods, and discloses probiotic tablets based on lactobacillus plantarum and a preparation method of the probiotic tablets. Lactobacillus plantarum freeze-dried powder, tea leaf theanine, konjaku flour, direct compression auxiliary materials, vitamin C and magnesium stearate are compounded, the direct compression auxiliary materials are adopted, and a direct compression technique is adopted, so that process flows for granulation and the like can be omitted, and the energy consumption can be reduced, the productivity can be increased, and the properties of the tablets can also be improved notably. The probiotic tablets can reduce weight, reduce in vivo fat accumulation, reduce ratio of internal organs of the body and body fat ratio, reduce leptin level and reduce blood lipid, and can be used for preventing and treating obesity. In addition, lactobacillus plantarum 1701 contained in the tablets also has favorable tolerance and adhesion property. The bacterial strains after being inactivated also have the function of reducing weight, so that products containing the bacterial strains are good in stability and long in quality guarantee period.

The invention discloses lactobacillus helveticus with a depression improving function. The lactobacillus helveticus is named as WHH1889, is separated from milk residues collected from country of Shigatse city in Tibet Autonomous Region in China, is preserved in China General Microbiological Culture Collection Center on February 25, 2021, and has a preservation number of CGMCC NO.21832. The microbial classification is named as Lactobacillus helveticus. The lactobacillus helveticus strain has good tolerance, adhesiveness and intestinal barrier protection performance, can effectively relieve depression / anxiety-like behaviors of depression mice and callback the level of depressive nerve factors, is excellent in fermentation characteristic, can be applied to fermented milk and can also be used for drugs, health care products or healthy food for preventing and treating depression.

The invention discloses a lactobacillus capable of adsorbing or degrading enterotoxin and an application thereof. The preservation number of the lactobacillus is CCTCC No: M2015128. The lactobacillus can adsorb or degrade enterotoxin and has the good effects of resisting acid, cholate, artificial gastric juice and artificial intestinal juice. The lactobacillus is specifically characterized in that the enterotoxin can be adsorbed or degraded, and good enterotoxin removal capacity is achieved; growth of common pathogenic bacteria can be restrained; the lactobacillus can resist acid and cholate and can survive in the digestive tract. In specific application, a method includes the step that freeze-dried bacterial powder is dissolved in water at normal temperature to be taken orally or is directly added according to a certain proportion to make functional food or healthcare products. The lactobacillus is screened from multiple lactobacilli, has the high enterotoxin removal capacity and good performance, and can be used for human body healthcare.

The invention provides a microbial compound bacterial agent. The microbial compound bacterial agent comprises lactobacillus brevis, bifidobacterium longum, saccharomyces bayanus and streptococcus faecium. The microbial compound bacterial agent has the advantages that the ability to inhibit pathogenic bacteria is strong, and the survival rate is high after the microbial compound bacterial agent isprepared into freeze-dried powder; four strains of the microbial compound bacterial agent have no mutual antagonistic effects and can be mutually-beneficial and symbiotic, the bifidobacterium longum,lactic acid bacillus and the streptococcus faecium all have strong antagonistic effects on the pathogenic bacteria, and the microbial compound bacterial agent formed by the four strains has good functions of regulating the gastrointestinal health and improving immunity.

The invention provides bifidobacterium longum, which is submitted to CGMCC for storage in July 17, 2018, with the preservation number of CGMCC No.16126. The bifidobacterium longum provided by the invention is strong in pathogenic bacteria inhibiting capability and high in survival rate after being prepared into freeze-dried powder.

The invention provides lactobacillus casei with an effect of reducing blood pressure and application of the lactobacillus casei, and belongs to the technical field of functional bacteria. The invention provides lactobacillus casei SLK30, which is preserved in the Guangdong Microbial Culture Collection Center, the address is 59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou City, the preservation date is March 9, 2022, and the preservation number is GDMCC No: 62280. The Lactobacillus casei SLK30 provided by the invention has ideal ACE inhibition rate and GABA production capability, and is a probiotic strain for lowering blood pressure. Fermentation characteristic research shows that the lactobacillus casei SLK30 has relatively good acid production capacity, acid resistance and bile salt resistance, has a relatively strong inhibition effect on indicator bacteria staphylococcus aureus, escherichia coli and salmonella typhimurium, and can be used as a potential antihypertensive probiotic to be applied to fermented food.

The present invention relates to the field of microorganisms, and discloses a strain of Lactobacillus rhamnosus that can reduce blood uric acid, which is named: 1155, and was preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee on January 4, 2016. The microbial preservation number is CGMCC NO.11955; the address of the preservation unit is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the microbial classification is named Lactobacillus rhamnosus . The bacterial strain of the invention can effectively reduce the content of blood uric acid. In addition to reducing the intake of exogenous purine substances, it can also promote the excretion of uric acid in the body from the intestines, and more effectively control the body's blood uric acid level.

The invention provides a preparation method of a probiotic composition for preventing type II diabetes. The preparation method comprises the following steps: A, inoculating lactobacillus plantarum N3117 into an MRS liquid culture medium, and carrying out anaerobic enrichment culture at 35-40 DEG C for 44-52 hours to obtain a fermentation bacterial solution; B, centrifugally separating the fermented bacterial solution, and collecting precipitates to obtain bacterial sludge; C, adding a freeze-drying protective additive solution into the bacterial sludge, performing vacuum freeze-drying after emulsification, and performing crushing and sieving to obtain lactobacillus plantarum N3117 bacterial powder; and D, mixing the lactobacillus plantarum N3117 bacterial powder with pumpkin powder, Chinese yam powder, inulin and citric acid powder to obtain the probiotic composition. The probiotic composition adopted by the invention has the basic characteristics and biological functions of lactobacillus plantarum N3117, and compared with lactobacillus plantarum N3117 bacterial powder, the probiotic composition has the advantages of reasonable formula ratio, full play of the functionality of each component, high viable count, strong stability, easy preservation and the like.