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Lactobacillus kefiri KL22 as well as screening method and application thereof

A technology of Lactobacillus kefir and a screening method, which is applied in the field of bioengineering, can solve the problems of cumbersome operation steps, easy to cause pollution between batches, and large differences, and achieve the characteristics of good intestinal regulation, acid resistance and bile salt resistance , Good effect of inhibiting the invasion of pathogenic bacteria

Inactive Publication Date: 2015-04-29
JUNLEBAO DAIRY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, reports at home and abroad show that the fermented milk fermented with kefir grains has the effect of inhibiting harmful bacteria, but the special taste of fermented milk prepared by using kefir grains is unacceptable to consumers in my country, and directly adopts kefir grains to prepare fermented milk. Large-scale industrial production of Phil grains, the operation steps are cumbersome, easy to cause pollution and large differences between batches

Method used

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  • Lactobacillus kefiri KL22 as well as screening method and application thereof
  • Lactobacillus kefiri KL22 as well as screening method and application thereof
  • Lactobacillus kefiri KL22 as well as screening method and application thereof

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Effect test

Embodiment １

[0040] Example 1 Lactobacillus kefir KL22 and its screening method

[0041] Lactobacillus kefir KL22, this strain has been preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee on September 16, 2014, and the preservation number is CGMCC No.9669.

[0042] Its 16SrDNA sequence results are as follows:

[0043] TTACCTCACCGGCTTTGGGTGTTACAAACTCTCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCAACTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGAACGGCTTTAAGAGATTAGCTTGACCTCGCGGTTTCGCGACTCGTTGTACCGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTTGCTAGAGTGCCCAACTGAATGCTGGCAACTAACAATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCATTCTGTCCCCGAAGGGAACGCCTAATCTCTTAGGTTGGCAGAAGATGTCAAGACCTGGTAAGGTTCTTCGCGTAGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTGAAGGGCGGAAACCC...

Embodiment 2

[0108] Embodiment 2 The preparation of the lactic acid bacteria beverage containing kefir lactic acid bacteria KL22

[0109] Mix 50 parts of pure milk, 30 parts of water, and 5 parts of sucrose evenly and sterilize at 110°C for 15 minutes to prepare the culture medium;

[0110] will be 10 6 cfu / ml Lactobacillus kefir KL22, 10 6 cfu / ml of Lactobacillus bulgaricus and 10 6 Streptococcus thermophilus of cfu / ml was inserted into the culture medium and mixed evenly, fermented at 37°C until the pH reached 4.2-4.6, the fermentation was terminated, and the filling was cooled. Keep refrigerated.

Embodiment 3

[0111] Embodiment 3 Preparation of probiotic bacteria powder containing kefir lactic acid bacteria KL22

[0112] Ingredients: Lactobacillus kefir KL22 3%, Lactobacillus plantarum 3%, Lactobacillus acidophilus 1%,

[0113] Bifidobacterium lactis 1%, Lactobacillus casei 2%, fructooligosaccharides (prebiotics) 80%, strawberry extract 10%;

[0114] The preparation process of the probiotic powder is as follows: Weigh the powder, fructooligosaccharide, and strawberry extract according to the formula ratio, mix them, and pack them on a vacuum packing machine, 2g / bag (wherein the total number of active lactic acid bacteria is ≥4×10 10 cfu / bag), labeled for storage, and stored in refrigeration.

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Abstract

The invention discloses a lactobacillus kefiri KL22 as well as a screening method and application thereof. The strain is collected in China General Microbiological Culture Collection Center (CGMCC) on September 16, 2014 and has a collection number of CGMCC No. 9669. Screened kefiri grains are sequentially subjected to separation, purification, a sugar fermentation characteristic experiment, a molecular biology identification, acid and bile salt tolerance characteristic experiment, an intestinal cell adhesion characteristic experiment, a harmful bacteria inhibition experiment and a strain fermentation characteristic experiment to finally screen lactobacillus kefiri KL22. The screened KL22 can be used for preparing yogurt, lactic bacteria drinks and bacterial powder preparations containing lactobacillus kefir KL22. The method is suitable for screening the lactobacillus kefiri KL22 which is used for preparing functional dairy products and probiotics powder containing lactobacillus kefiri KL22.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a lactobacillus and a screening method thereof, in particular to a lactobacillus kefir KL22, a screening method and an application thereof. Background technique [0002] At present, the only probiotics that can be used in food in my country are Bifidobacterium, Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacillus casei subspecies cheese, and Streptococcus thermophilus. Kefir fermented milk has many probiotic functions. Kefir grains contain a variety of physiological beneficial bacteria, which adhere and colonize in the intestinal tract and compete with pathogenic bacteria for adhesion sites and nutrients. The metabolized products of lactic acid and acetic acid can reduce intestinal pH value; the antibacterial substances hydrogen peroxide, bacteriocin, etc. produced can effectively inhibit the colonization of pathogenic microorganisms in the intestinal tract. Therefore, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/127C12R1/225
CPCA23C9/127C12N1/20C12N1/205C12R2001/225
Inventor 朱宏王华王世杰冯丽莉薛玉玲
Owner JUNLEBAO DAIRY GRP CO LTD
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