814 results about "Doderlein's bacillus" patented technology

The invention relates to a water improver with multiple active microbes and a preparation method thereof, which adopts two or a plurality of following microbial strains, comprising a bacillus licheniformis, a bacillus subtilis, a bacillus megaterium, a lactobacillus plantarum, a saccharomyces cerevisiae, a candida lipolytica, bacillus pumilus, bacillus cereus, a rhodopsedomnas palustris, an alcaligenes faecalis and an arthrobacter globiformis. After the femination of the microbial strains, the preparation of the liquid is that the single microzyme zymocyte liquid is mixed and stirred in proportion; or the mixed bacteria solution is concentrated; or the concentrated single microzyme zymocyte liquid is mixed and stirred according to the proportion of the single microzyme zymocyte liquid, and is prepared into a mix bacteria solution. The mix bacteria solution is mixed homogeniously with an added assistant agent. The total amount of the live bacteria within the warrenty period is 106 to 1010 per milliliter. The preparation of the solid inoculant is that the zymocyte liquid is dried after the absoption of the adsorbent on the zymocyte liquid, is prepared into a solid bacterial powder and mixed evenly with the added assistant agent. The total amount of the live bacteria within the warreny period ranges between 106 and 1011 per gram. The invention has multi-function and stable effect and is suitable for treating various water bodies.

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The invention provides a lactobacillus helveticus microcapsule as well as the preparation and application thereof, pertaining to the embedding technology of lactobacillus helveticus and aiming at microencapsulating the lactobacillus helveticus so as to solve the problems of low embedding efficiency and embedding yield in single-layer or double-layer embedment. The technical proposal adopted by the invention is that isolated soy protein, microporous starch and sodium alginate are respectively taken as the first, the second and the third layers of wall materials for microencapsulation; the three-layer embedment is carried out to the lactobacillus helveticus for the first time, wherein, in the process of preparing bacterial suspension, an orthogonal optimization protective agent is adopted to improve the livability after freeze drying. The microcapsule of the invention has strong heat resistance and acid resistance, solves the inactivation problem caused by gastric acid, digestive enzyme and antibiotic, etc. to active bacillus, is capable of releasing when reaching large intestine, and balances intestinal flora. The reusability of fermented milk is good. The lactobacillus helveticus used by the invention enriches the variety of probiotic bacteria microcapsule and is a good dietary supplement.

The invention discloses a microecological preparation, a preparation method and applications thereof, and relates to the field of aqua culture. The microbiological preparation comprises at least one component selected from bacillus subtilis and bacillus pumillus, and at least one component selected from lactobacillus plantarum and lactobacillus acidophilus; wherein the bacillus subtilis comprises Bacillus subtilis 1A05680, the bacillus pumillus comprises Bacillus pumilus 1A08151, the lactobacillus plantarum comprises Lactobacillus plantarum 1A07806, and the lactobacillus acidophilus comprises Lactobacillus acidophilus PL7. The microecological preparation can be applied to preparation of preparations, which are used for promoting growth of prawn or grouper, improving immunity and disease resistance of prawn or grouper, preventing and treating diseases, increasing the survival rate, and purifying the culture water.

The invention relates to a feeding addition agent for microorganism viable bacteria and a preparing method thereof. The addition agent consists of 1.5-2.5 parts of bacillus coagulans viable bacteria powder, 0.5-2.0 parts of lactobacillus acidophilus viable bacteria powder, 1.0-2.5 parts of lactobacillus plantarum viable bacteria powder, 0.5-2.5 parts of bacillus natto viable bacteria powder, 0.5-1.5 parts of candida utili viable bacteria powder and 2-4.5 parts of aspergillus niger viable bacteria powder. Each gram of the content of viable bacteria is as follows: bacillus coagulans is not less than 1.0*10cfu / g; lactobacillus acidophilus is not less than 1.0*10cfu / g; lactobacillus plantarum is not less than 1.0-10cfu / g; bacillus natto is not less than 1.0*10cfu / g; aspergillus niger is not less than 1.0*10cfu / g; and candida utili is not less than 1.0*10cfu / g. The invention has the advantages that the composition is reasonable, the preparation method is scientific, the usage scale is wide, the operation is simple and easy to master, and the cost is low. In addition, the invention can be used as the feeding stuff agent for various poultries, thus increasing the use ratio of the feeding stuff addition agent and strengthening the anti-disease ability of the poultries.

The invention relates to a composite microorganism livestock and poultry feed additive and manufacturing method thereof, belonging to the feed technology field. The invention discloses a composite microorganism livestock and poultry feed additive, which is characterized in comprising composite bacterium powder in 1.0-15% of total mass protion; wherein, the composite bacterium powder is compatible of any two or more of the lactobacillus, yeast, pediococcus acidilactici and fecal streptococcus. the strains in the microorganism feed additive of the invention can symbiose to each other, which is favorable to performance their biological activities, and can keep enough shelf life and live bacteria quantity. The invention also achieves the advantages of controlled production process, lower cost and stable product quality.

The invention discloses complex microbial inoculant which comprises the following active ingredients: bacillus subtilis, bacillus megatherium, trichoderma koningii, aspergillus oryzae, trichoderma viride, geotrichum candidum, aspergillus niger, rhizopus oligosporus, lactobacillus plantarum CICC20768 and azotobacter chroococcum. Correspondingly, the invention further discloses a method for preparing an organic fertilizer from the complex microbial inoculant. The method comprises the following steps: a. uniformly mixing the complex microbial inoculant with a plant fiber raw material to obtain a fermented raw material; b, composting and fermenting the fermentation raw material; c, drying the composted fermentation raw material to obtain the organic fertilizer. microbes of the complex microbial inoculant have no antagonism, and can effectively cooperate in the production process of the organic fertilizer, and the chemical components of the production raw materials can be sufficiently utilized to finally produce the high-quality organic fertilizer. The produced organic fertilizer reaches the parameter indexes of the standard (NY884-2012) of China, can improve the planting property of soil, is beneficial to optimization of soil microbial flora, and can provide comprehensive nutrients to a plant root system.

Variant of the bacterium Lactobacillus fermentum and its use in treating gastrointestinal disorders, disorders of the mucosal surfaces or disturbances in the immune system or status of a subject.

The invention provides a new Lactobacillus plantarum bacterial strain YJG which was preserved in the China General Microbiological Culture Collection Center (CGNCC) with the collection number of CGMCC No. 2994 on march, 30th, 2009. The Lactobacillus plantarum bacterial strain YJG can produce broad-spectrum bacteriostatic activity bacteriocin with high yield, and can express bacteriostasis for staphylococcus aureus, salmonella and E.coli of gram-negative bacterium, L. plantarum, L.brevis, Streptococcus and Bacillus of gram-positive bacterium, and the like. A virus attacking tests with high dosage proves that the Lactobacillus plantarum bacterial strain YJG is safe to animal bodies. The Lactobacillus plantarum bacterial strain YJG and a zymophyte liquid thereof can be used for preparing additives of green animal feeds, and the bacteriocin of the Lactobacillus plantarum bacterial strain YJG can be used for preparing green biological veterinary drugs, environmental-friendly biopreservatives, biocontrol drugs and the like.

The invention relates to the preparation of a Lactobacillus casei Zhang strain and an application of the strain at the aspect of antioxidant products. The Lactobacillus casei Zhang strain is an acid- and bile acid-resistant probiotics strain separated from Lactobacillus casei Zhang of fermented mare milk which is the traditional dairy product of the inner Mongolia nationality; and the strain has been stored in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, and the Collection No. is: CGMCC No.1697. Besides the beneficial function to body health possessed by common lactobacillus, the Lactobacillus casei Zhang of the invention can also be used for the production of anti-oxidation functional products, such as: the production of various dairy products with antioxidant activity; and the Lactobacillus casei Zhang can be present in specific products by mixing with other materials, and can also be produced in the form of live bacteria preparation.

The invention discloses a mycotoxin degrading adsorbent, which contains bacillus subtilis, lactobacillus plantarum, lactobacillus acidophilus, leuconostoc lactis, lactobacillus delbrueckii, lactobacillus fermenti, lactobacillus salivarius, vitamin C, vitamin E, radix astragali, zinc methionine, potassium chloride, cassia bark, clove, oriental wormwood, folium artemisiae argyi, sweet wormwood, wild chrysanthemum flower, semen plantaginis, yeast cell wall and phytase. The mycotoxin degrading adsorbent disclosed by the invention has multiple effects including generation inhabitation, degradation and adsorption on various common mycotoxins, and can additionally boost immunities of pigs and promote excretion of toxic substances; the mycotoxin degrading adsorbent has excellent comprehensive performance and plays a remarkable role on productivities of piglets, and the mycotoxin degrading adsorbent can reduce disease rate, improve daily gain, increase feed digestibility and reduce feed conversion ratio. Moreover, the mycotoxin degrading adsorbent is low in dosage but effective and free from adsorption on drugs and other nutrient substances, and the adsorbent can be biologically degraded after being secreted and avoid pollutants; and the mycotoxin degrading adsorbent has good application value in breeding industry.

The invention provides a composite active bacterial preparation which is used for aquatic products and has the water improving and fertilizing functions and a preparation method thereof by applying a modern bioengineering technology mainly for the problem that in existing aquaculture, the water quality of a pond is poor. The composite microecological preparation is prepared by mixing bacillus subtilis, bacillus megatherium, bacillus amyloliquefaciens, bacillus licheniformis, enterococcus faecium, lactobacillus brevis, lactobacillus plantarum, lactococcus lactis and saccharomyces cerevisiae through liquid-submerged fermentation. In the liquid preparation, microorganisms are in a dormant state and are stable and easy to preserve, after the preparation is put into water, the dormant strains can quickly resuscitate, harmful substances such as nitrite nitrogen and ammonia nitrogen in the water are quickly decomposed, good algae growth is facilitated, harmful algae reproduction is inhibited, and therefore the purpose of improving the water quality of the culture water is achieved.

The invention discloses a microbial deodorizing microbial inoculum for public toilets, livestock and poultry farms and refuse collection points. The microbial deodorizing microbial inoculum contains pichia jadinii with the preservation number of CGMCCNO.5982, issatchenkia orientalis with the preservation number of CGMCCNO.6496, lactobacillus sp. with the preservation number of CGMCCNO.6495, streptococcus thermophilus with the preservation number of CGMCCNO.5985, bacillus licheniformis with the preservation number of CICCNO.20085 and streptomyces microflavus var. lactosus with the preservation number of CGMCCNO.4891. The invention also discloses a preparation method of the microbial deodorizing microbial inoculum in both liquid and solid states, and application of the microbial deodorizing microbial inoculum.

The invention provides a high-efficiency compound microbial inoculum for fermenting beds, particularly relates to a compound microbial inoculum for the fermenting beds, and a preparation method and application thereof. The compound microbial inoculum is composed of five beneficial bacterium strains (Bacillus subtilis CGMCC1.769 and CGMCC1.210, lactobacillus plantarum CICC6073, Candida tropicalis CGMCC2.587 and Trichoderma koningii CGMCC3.4290) and metabolites thereof (amylase, protease, lipase, cellulase, organic acid, bacteriophage and the like). The compound microbial inoculum is prepared by using a submerged liquid mixed fermentation technique. The method comprises the following steps: carrying out liquid submerged fermentation on the lactobacillus plantarum at 35 DEG C for 12 hours, respectively inoculating Candida tropicalis and Trichoderma koningii, carrying out mixed fermentation at 30 DEG C for 12 hours, inoculating Bacillus subtilis, carrying out liquid submerged mixed fermentation for 24 hours, respectively controlling the fermented viable count at 1-2*10<9> cfu / mL, 5-8*10<8> cfu / mL, 3-5*10<8> cfu / mL, 3-5*10<9> cfu / mL and 2-5*10<9> cfu / mL, finishing fermentation, packaging and sealing to obtain the high-efficiency compound microbial inoculum for the fermenting beds. The invention also provides application of the high-efficiency compound microbial inoculum-a preparation method of padding material. The compound microbial inoculum has the advantages of high viable count, high biological activity and low impurity bacterium pollution; the compound microbial inoculum can obviously reduce the odor of pigsties and reduce the living possibility of mosquitoes and flies, thereby achieving the ecological culture goals of no pollution and zero discharge; and the process for manufacturing the ferment bed is simple, thereby saving the padding material, human power and the like.

The invention relates to an application of lactobacillus plantarum in preparation of food, health food, a health product or a medicine with a function of reducing blood fat or assisting fat-reducing. The collection number of the lactobacillus plantarum is CGMCC No.8198. the lactobacillus plantarum was collected in China General Microbiological Culture Collection Center (CGMCC) on Sep. 17, 2013. The address is 1 Beichen West Road, Chaoyang District, Beijing. The strain achieves the effect of reducing blood fat by reducing the level of serum cholesterol, triglyceride and low-density lipoprotein cholesterin. L.plantarum CGMCC No.8198 and its fermented products also play a role in controlling and reducing weight, and have an effect of assisting fat-reducing. The invention provides a biological therapy for effectively reducing serum cholesterol and assisting fat-reducing. The biological therapy has long-term eating safety and has a considerable application prospect.

A feed additive composition comprising a direct fed microbial (DFM), in combination with a xylanase (e.g. endo-1,4-β-d-xylanase) and a β-glucanase (and optionally a further fibre degrading enzyme), wherein the DFM is selected from the group consisting of an enzyme producing strain; a C5 sugar-fermenting strain; a short-chain fatty acid-producing strain; a fibrolytic, endogenous microflora-promoting strain; or combinations thereof. The DFM may be selected from the group consisting of: Bacillus subtilis AGTP BS3BP5, Bacillus subtilis AGTP BS442, B. subtilis AGTP BS521, B. subtilis AGTP BS918, Bacillus subtilis AGTP BS1013, B. subtilis AGTP BS1069, B. subtilis AGTP 944, B. pumilus AGTP BS 1068 or B. pumilus KX11-1, Enterococcus faecium ID7, Propionibacterium acidipropionici P169, Lactobacillus rhamnosus CNCM-1-3698, Lactobacillus farciminis CNCM-1-3699, a strain having all the characteristics thereof, any derivative or variant thereof, and combinations thereof and the further fibre degrading enzyme may be selected from the group consisting of a cellobiohydrolase (E.C. 3.2.1.176 and E.C. 3.2.1.91), a β-glucosidase (E.C. 3.2.1.21), a β-xylosidase (E.C. 3.2.1.37), a feruloyl esterase (E.C. 3.1.1.73), an α-arabinofuranosidase (E.C. 3.2.1.55), a pectinase (e.g. an endopolygalacturonase (E.C. 3.2.1.15), an exopolygalacturonase (E.C. 3.2.1.67) or a pectate lyase (E.C. 4.2.2.2)), or combinations thereof.

The present invention discloses lactobacillus rhamnosus, named lactobacillus rhamnosus HCS01-003. The strain is preserved on August 14, 2018 in the "China General Microbiological Culture Collection Center", and the preservation number is CGMCC No.16257. The invention also discloses an application of the strain used as an additive lactobacillus rhamnosus lyophilized powder which has strong anti-digestive tract anti-environment ability and good bacteriostatic property in preparing foods, health foods or pharmaceutical compositions. The invention further discloses an application of the strain used as a food additive lactobacillus rhamnosus lyophilized powder which has strong anti-digestive tract anti-environment ability and good bacteriostatic property in preparing infant foods.

The invention belongs to the field of microbes, and concretely relates to a lactic acid bacterium with wide-spectrum bacteriostatic activity and application thereof. The lactic acid bacterium with wide-spectrum bacteriostatic activity is named lactobacillus salivarius GZPH2, is preserved at China Center for Type Culture Collection (CCTCC) since 27th, November, 2014, and has the preservation number of CCTCC M 2014598. The provided lactic acid bacterium lactobacillus salivarius GZPH2 has good bacteriostatic effect on multiple pathogenic bacteria of human and aquaculture bionts, and possesses the characteristics of not generating histamine, no hemolysis, acid resistance, cholate resistance and the like.

The invention relates to a composite starter for sourdough steamed buns. The composite starter is a composition of fermentation strains including saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus brevis, wherein the bacterial number ratio of the saccharomyces cerevisiae to the lactobacillus plantarum to the lactobacillus brevis is (1 to 10): (1 to 5): 1. According to the composite starter for the sourdough steamed buns, which is disclosed by the invention, a strain is separated from the traditional sourdough, and a natural bacterium mixing system of the traditional starter is simulated, thus the traditional unique and abundant flavour can be given to the steamed buns, and the defect of insufficient flavour of the existing starter is made up; and the flavour and taste of the steamed buns can be improved, and the functions of increasing the nutritional value of the steamed buns and prolonging the shelf life of products are further realized due to the existence of lactic acid bacteria. The composite starter disclosed by the invention is simple in production procedures, the microbial inoculum is high in fermentation activity, and the steamed buns are stable in quality.

The invention relates to a complex microorganism additive as well as a preparation method and application thereof, belonging to the biological material technical field of application of a microecological modulator in cultivating domestic animals. The preparation method thereof comprises the following steps: taking 30-60% of Saccharomyces cervisiae, 5-30% of Candida utilis, 5-30% of Rhodopseudom onas Palustris, 5-30% of Lactobacillus plantarum, 5-40% of Leatobacillus acidophilus and 5-25% of Bacillus subtilis; and sequentially carrying out primary bacteria strain culture, secondary fermentation and tertiary fermentation on the bacteria strains so that the bacterial number in fermentation liquor reaches 8-10*108 / mL, thus obtaining the complex microorganism additive 808. Long-term on-site experiments prove that the product has reasonable formula, stable efficacy, disease prevention and resistance, improved quality and capability of promoting growth of animals and improving yield of animal products, survival rate and feed conversion rate.

The invention relates to the field of microorganisms and discloses lactobacillus plantarum with a weight reducing function and application thereof. The lactobacillus plantarum 1701 strain is separatedfrom a yogurt powder sample collected from rural areas of Shigatse in the Tibet Autonomous Region. The strain is preserved in the China General Microbiological Culture Collection Center on October 23, 2019, and the microorganism has the preservation number of CGMCC NO.18728. The lactobacillus plantarum 1701 has excellent tolerance and adhesion, can remarkably reduce the weight of a rat, reduce fat accumulation in a body, reduce the viscera ratio and body fat ratio, reduce the leptin level and reduce blood lipid and can be used in medicines, health-protecting products and health foods for preventing and treating obesity. Furthermore, the strain still has a weight reducing function after inactivation, thus having rich product mode, wide application range, high product stability and long preservation period.

The invention discloses a lactobacillus pentosus strain and ferment produced thereby, wherein the docket number of the R1(Lactobacillus pentosus R1) strain in the Chinese Microbiological Culture Preservation Administration Commission Common Microbiological Center is CGMCC No.0917. The pentose lactobacillus R1 strain of the invention can endure 4.0% salt solution and nitrite solution whose concentration is 80-100mg / L. The leaven prepared from the bacterial can be applied into the fermentation production of meat food.

The invention relates to a lyophilization process of lactobacillus plantarum, and belongs to a microbial agent preparation process. In the lyophilization process of lactobacillus plantarum, the process parameters are that the pH of a lyophilization protective agent is 7.0-7.5, the balancing time of bacterium mire and the protective agent is 20-40minutes, pre-lyophilization is carried out for 2.0-2.5h under the condition of minus 80 DEG C, a phosphate buffer is adopted to prepare the protective agent, lyophilization livability can be up to over 90%, and the viable count can be up to over 4.0*1010cfu / g. Compared with the general method, the method disclosed by the invention has the advantages that the livability of the lactobacillus plantarum is improved remarkably.

The invention provides a fermented straw compound microbial agent and a preparation method and application thereof. The microbial agent is prepared from enterococcus faecium WEI-6, bacillus subtilis WEI-29, bacillus licheniformis WEI-30, lactobacillus plantarum WEI-64 and brewer's yeast WEI-92; the total amount of bacillus in compound bacterial liquid is 1.5*109 CFU / mL, the total amount of lactic acid bacteria is 2.5*109 CFU / mL, and the total amount of yeast is 2.07*108 CFU / mL; the compound bacterial liquid is inoculated into large-scale compost with straw and livestock excrement as the main raw materials, a certain amount of agricultural waste is added at the same time, and the excellent organic fertilizer can be obtained through fermentation maturity. The bacterial strain of the compound microbial agent is obtained from medium-temperature dominant bacterial community in a natural fermentation process of the straw compost, the bacterial strain can effectively improve an organic fertilizer fermentation production process with the straw and the livestock excrement as the main raw materials after being screened and acclimatized for a long time, and the production efficiency of the organic fertilizer and the innocent treatment effect of the straw and the excrement are improved; thus, the fermented straw compound microbial agent is suitable for livestock and poultry farms with large-scale straw resources nearby to perform innocent treatment and resource utilization of the straw and the cultivation excrement.

The invention discloses a plant microecological preparation for enhancing the immunity of an animal and a preparation method of the plant microecological preparation, and belongs to the field of feed additives. The plant microecological preparation is prepared by the following steps: mixing crushed materials or extracted materials of radix pseudostellariae, liquorice, rhizoma atractylodis macrocephalae, honeysuckle, astragalus membranaceus, eucommia ulmoides, great burdock achene, dandelion and herba epimedii, adding a culture solution to prepare a culture medium, adding a total bacterium liquid consisting of two or more than two of lactobacillus plantarum, enterococcus faecalis, enterococcus faecium, enterococcus faecium, pediococcus acidilactici, pediococcus pentosaceus, rhodopseudomonas palustris, bacillus subtilis and lactobacillus fermenti, fermenting and drying. The plant microecological preparation disclosed by the invention has the advantages that the immunity of the animal can be obviously improved, the production performance can be improved and the diarrhea rate can be reduced.

The invention relates to a multi-strain microorganism pig feed additive and a production method thereof. According to the additive, bacillus subtilis, lactobacillus delbrueckii, saccharomyces cerevisiae, candida and photosynthetic bacteria are inoculated into a fermentation culture medium according to a ratio of 2:1:1:1:2, and are prepared into a finished product through solid fermentation. The microorganism preparation is reasonably matched, various microorganisms in the preparation can bring respective effects into play and depend on one another, the overall advantage of the microorganism preparation can be brought into play, the growth development and the improvement of the anti-disease capability of a host are facilitated, and the feed additive is safe to use and can improve the immunity of animals, prevent and resist diseases, prompt animal growth, improve the feed utilization rate and improve the feed quality.

The invention discloses a lactobacillus fermentum strain suitable for table vinegar brewing, wherein the classification name of the lactobacillus fermentum strain is Lactobacillus fermentum HS3-7, the Lactobacillus fermentum HS3-7 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation place is the Institute of Microbiology of Chinese Academy of Sciences located in 3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing, the accession number is CGMCC NO.12226, and the preservation data is March 18, 2016. The invention further provides ready-to-use bacteria powder of the lactobacillus fermentum strain and applications of the bacteria powder. After the bacteria powder is used, the number of lactobacillus in table vinegar or other vinegar products can be obviously improved, the content of non-volatile acid is increased, and the flavor and quality of the product are improved.

The invention discloses a method of producing a protein feed by a liquid-solid two-step fermentation method. The method comprises the following steps: performing liquid culture on corynebacterium glutamicum, candida utilis, bacillus subtilis, lactobacillus plantarum and aspergillus oryzae so as to obtain corynebacterium glutamicum fermentation liquor, candida utilis fermentation liquor, bacillus subtilis fermentation liquor, lactobacillus plantarum fermentation liquor and aspergillus oryzae fermentation liquor; mixing the corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the bacillus subtilis fermentation liquor, the lactobacillus plantarum fermentation liquor and the aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of soybean meal, cluster bean meal, cottonseed meal and the like into an explosion vat for performing steam explosion; after steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an enzyme preparation so as to obtain mixed materials; conveying the mixed materials into a special modularized solid-state biological raw material continuous fermentation device for enzymolysis and fermentation, and drying the fermented materials so as to obtain the protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the enzyme preparation are mixed, then the mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic temperature control, automatic dampness control and automatic ventilation control are met, the bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.

The invention provides a probiotic composition and a preparation method and applications thereof, and belongs to the technical field of health-care foods. The probiotic composition comprises probiotics and prebiotics. The probiotics comprise lactobacillus acidophilus NCFM, lactobacillus rhamnosus HN001, lactic acid bacteria composite powder GI, lactic acid bacteria composite powder OH, bifidobacterium animalis B94, bifidobacterium bifidum R0071, lactobacillus casei R0215, live bacillus coagulans GBI30,6086 and lactobacillus rhamnosus GG. The prebiotics include fructo-oligosaccharides, resistant dextrins and lactitol. The mass ratio of the probiotics to the prebiotics is (300-350):(1300-1400). The total viable bacteria concentration of the probiotics in the probiotics composition is more than or equal to 9.0x10<9> CFU / g. The probiotic composition provided by the invention can obviously improve sleep quality, and significantly relieve anxiety and depression.