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Lactobacillus plantarum with high antioxidant activity and application of lactobacillus plantarum

A Lactobacillus plantarum, oxidative activity technology, applied in the field of Lactobacillus plantarum, can solve the problems of antioxidant safety, health threat, excessive free radical production, etc., achieves good acid resistance and bile salt resistance, easy to use, and enhances body immunity. force effect

Active Publication Date: 2015-11-04
山东凤凰生物科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although under normal circumstances, the body has a free radical scavenging system, if the body is affected by certain factors and the free radical scavenging system malfunctions, resulting in excessive free radicals in the body, it will act on molecules such as proteins and nucleic acids in the body, causing cell damage. injury, which poses a great threat to the health of the organism
The antioxidant capacity of the body can be enhanced by supplementing antioxidant substances in the diet, but many chemically synthesized antioxidants have safety problems

Method used

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  • Lactobacillus plantarum with high antioxidant activity and application of lactobacillus plantarum
  • Lactobacillus plantarum with high antioxidant activity and application of lactobacillus plantarum
  • Lactobacillus plantarum with high antioxidant activity and application of lactobacillus plantarum

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Experimental program
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Effect test

Embodiment 1

[0032] 1. Isolation and purification of lactic acid bacteria

[0033] Take an appropriate amount of kimchi and put it into 80mL MRS medium for enrichment culture at 37°C for 20h. 3 Stretch on the MRS agar medium, after static culture at 37°C for 24 hours, pick a single colony with an obvious transparent circle on the medium, and pick out the Gram stain positive and contact enzyme reaction through Gram staining and contact enzyme reaction. Negative strains were kept on slant.

[0034] 2. Screening of lactic acid bacteria with antioxidant activity

[0035] 2.1 Culture of lactic acid bacteria and preparation of fermentation broth

[0036] Lactic acid bacteria were cultured in MRS liquid medium at 37°C for 24h, centrifuged at 6000r / min at 4°C for 10min, and the supernatant was collected as the fermentation broth.

[0037] 2.2 Lactic acid bacteria in different concentrations of H 2 o 2 Tolerance determination in solution

[0038] Add 60mL of MRS liquid medium into a 100mL ste...

Embodiment 2

[0117] 1. Experimental strains

[0118] Lactobacillus plantarum BLCC2-0015 was selected.

[0119] 2. Medium

[0120] The formula of the seed liquid medium is: glucose 20g / L, peptone 10g / L, beef extract 10g / L and yeast extract 5g / L, adjust the pH to 6.0 (5.5-6.5 is enough), and sterilize at 115°C for 20 minutes; The solid slant medium is the agar powder added with 1.5% (all within the range of 1.5-2.0%) to the seed liquid medium.

[0121] The liquid fermentation medium formula is: glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, sodium acetate 5g / L, ammonium citrate 2g / L, dipotassium hydrogen phosphate 5g / L, Magnesium sulfate 0.5g / L, manganese sulfate 0.2g / L and Tween-801mL / L, when used, adjust the pH to 6.0 (5.5-6.5 range is enough).

[0122] 3. Experimental steps

[0123] (1) Inoculate the lyophilized powder strain on a solid slant medium, and culture it at 37°C for 24h to activate (30-37°C, 20-30h);

[0124] (2) Take the cultured solid slant medium,...

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Abstract

The invention discloses lactobacillus with high antioxidant activity. The lactobacillus named as lactobacillus plantarum BLCC2-0015 is preserved in China Center for Type Culture Collection on March 18, 2015 with the preservation number of CCTCC NO.M2015126. The lactobacillus with high antioxidant activity has the advantages that the lactobacillus is screened from numerous lactobacillus, the lactobacillus is high in antioxidant activity and good in acid and bile salt tolerance, the lactobacillus powder made by the lactobacillus can be produced into body antioxidant food or health care products which can be directly eaten or mixed with water for drinking, use convenience is achieved, and body immunity can be enhanced.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a plant lactobacillus with high antioxidant activity and its application. Background technique [0002] Biological oxidation is an important physiological process of the body's metabolism, but various reactive oxygen species (ROS) such as free radicals will be produced during this process. Although under normal circumstances, the body has a free radical scavenging system, if the body is affected by certain factors, the free radical scavenging system malfunctions and the excessive production of free radicals in the body will act on molecules such as proteins and nucleic acids in the body, causing cell damage. Injury poses a great threat to the health of the body. The body's antioxidant capacity can be enhanced by supplementing antioxidant substances in the diet, but many chemically synthesized antioxidants have safety problems. Lactic acid bacteria, as the resident flor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L1/29A61K35/747A61P39/06C12R1/25A23L33/00
Inventor 单宝龙王静徐海燕辛国芹刘中青汪孟娟董佩佩
Owner 山东凤凰生物科技股份有限公司
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