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Lactobacillus plantarum with bacteriostat characteristic and application thereof in diarrhea prevention

A Lactobacillus plantarum, characteristic technology, applied in the biological field, can solve the problems of serious strain homogeneity, lack of functional subdivision products, etc., and achieve the effects of good adhesion characteristics, reduced adhesion rate, and excellent bacteriostatic effect.

Active Publication Date: 2019-04-23
HANGZHOU WAHAHA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the domestic probiotics market has developed rapidly, but the product form is still dominated by fermented dairy products such as yogurt and lactic acid bacteria beverages, and the function claim is still mainly to regulate the function of the gastrointestinal tract and balance the intestinal flora, lacking further functional segmentation product
At the same time, the strains used in the domestic probiotics market basically rely on the monopoly supply of foreign companies such as DuPont and Chr.

Method used

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  • Lactobacillus plantarum with bacteriostat characteristic and application thereof in diarrhea prevention
  • Lactobacillus plantarum with bacteriostat characteristic and application thereof in diarrhea prevention
  • Lactobacillus plantarum with bacteriostat characteristic and application thereof in diarrhea prevention

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Antibacterial properties of Lactobacillus plantarum 589

[0032]The indicator bacteria were Micrococcus luteus, Escherchia coli CICC 23689, Staphylococcus aureus CICC 10301, Salmonella typhimurium CICC 10437, Shigella flexneri CICC 21534 ) four common intestinal infection pathogens. Using NB medium, under the condition of 37 ℃ culture, after three generations of continuous activation, it was used for antibacterial experiment. The antibacterial properties of the bacterial strains of the present invention were measured by a double-layer plate punching diffusion method. Pour the sterilized 1.5% agar into a sterile petri dish. After solidification, cool down the sterilized solid medium (containing 1.1% agar) to a certain temperature, mix it with the indicator bacteria and pour it into Placed on top of the agar layer in an Oxford cup. The indicator bacteria should be adjusted to a reasonable concentration according to the growth conditions. After the upper layer is solid...

Embodiment 2

[0041] Tolerance Characteristics of Lactobacillus plantarum 589

[0042] After culturing the strains of the present invention and commercially available commercial strains to the end of logarithmic growth, the bacterial suspension was obtained, and the bacterial suspension was divided into two groups, and the acid resistance and bile salt resistance were measured respectively. The operation is as follows. (1) Take a certain amount of bacterial liquid, centrifuge at 4000g / 10min and discard the supernatant. Add the same volume of MRS solution with pH=2.5, pipette and mix well, incubate at 37°C, and measure the changes in the number of bacteria at 0 point and after incubation for 1h, 2h, and 3h by the dilution smear counting method. (2) Take a certain amount of bacterial liquid, 4000g, centrifuge for 10min and discard the supernatant. Add the same volume of MRS solution containing 0.3% bile salts, pipette and mix well, incubate at 37°C, and measure the changes in the number of b...

Embodiment 3

[0046] Intestinal adhesion ability of Lactobacillus plantarum 589

[0047] strain

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PUM

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Abstract

The invention discloses a lactobacillus plantarum novel strain with the function of diarrhea prevention and intestinal tract protection. The strain has the restraining effect on common pathogenic entero becterium growth and intestinal tract planting, the strain has the good tolerance for gastric acid and chelate, can smoothly reach the intestinal tract and can be attached to intestinal tract epithelial cells, and the intestinal tract protection function is brought into play. The strain has the restraining effect on generation of pathogenic entero becteria, and the strain can better achieve theintestinal tract protection and diarrhea prevention functions due to the characteristics.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a new strain of Lactobacillus plantarum and the application of the strain in preventing diarrhea and protecting intestinal tract. Background technique [0002] Diarrhea is a common disease in people's daily life, showing seasonal and geographical differences. Summer and autumn are seasons with a high incidence of diarrhea. In addition to factors such as fatigue and cold, bacterial infection is an important cause of diarrhea in summer and autumn. In the hot and humid environment in summer and autumn, pathogenic microorganisms multiply quickly, and food is easy to deteriorate. If you do not pay attention to food hygiene, eating food contaminated by Escherichia coli, Shigella, Salmonella, Campylobacter, Vibrio, etc. cause diarrhea symptoms of varying degrees. On the other hand, with the improvement of people's living standards, traveling even abroad has gradually become a part of peop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A61P1/12A61P1/00A23L29/00A23L33/135A23L2/38A23L2/52A23K10/12A23K10/18C12R1/25
CPCA61P1/00A61P1/12A23K10/12A23K10/18A23L2/382A23L2/52A23L29/065A23L33/135C12N1/20A23V2002/00A61K2035/115C12R2001/25C12N1/205A23V2400/169A23V2200/3204
Inventor 陈作国朱珺李言郡孙盛陈苏
Owner HANGZHOU WAHAHA TECH
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