Preparation method of probiotic granules being resistant to acid and bile salt

Abstract

The invention relates to the field of probiotics, in particular to a preparation method of probiotic granules being resistance to acid and bile salt. The preparation method comprises the following steps of S1, uniformly mixing probiotic bacterial sludge with a protective agent, and performing slow stirring to obtain an emulsion; S2, adding prepared colloid liquid to the obtained emulsion, uniformly mixing and stirring the obtained emulsion with the colloid liquid, and performing slow stirring to obtain mixing liquid; S3, slowly injecting the obtained mixing liquid into a calcium chloride solution, performing a crosslinking reaction, after the mixing liquid is completely injected, performing high-speed shearing and stirring, and then performing standing to obtain mixed bacteria starch; andS4, performing freeze drying on the obtained mixed bacteria starch to obtain solids of which the water content is lower than 5%, and grinding and granulating the obtained solids by a grinding and granulating machine to obtain 20-50-mesh probiotic granules, wherein the protective agent comprises trehalose, glycerine and modified starch, and the colloid liquid comprises sodium alginate. The probiotic granules obtained through the preparation method disclosed by the invention have favorable acid resistance and bile salt resistance, and when the probiotic granules achieve intestinal tracts throughstomachs, the activity can be maintained to a great extent.

Description

Technical field [0001] The invention relates to the field of probiotics, in particular to a preparation method of acid- and bile-resistant probiotic particles. Background technique [0002] Probiotics are a type of bacteria that can promote human health. Common probiotics include Lactobacillus, Bifidobacterium and Streptococcus. For example, lactobacilli are mainly colonized in the human small intestine, and bifidobacteria are mainly colonized in the human large intestine. If a compound strain is used, it can have a comprehensive regulatory effect on the human body. The organic acids, polysaccharides, bacteriocins and special enzymes produced by the metabolism of these probiotics can regulate the balance of gastrointestinal flora, improve immunity, delay aging, improve constipation, diarrhea and allergic symptoms. [0003] The rise of modern human living standards, environmental pollution and drug abuse have caused many negative effects on the human body's microecological balance ...

AI Technical Summary

Problems solved by technology

[0004] At present, probiotic preparations mostly exist in the form of powders, tablets, hard capsules, granules, etc. in the field of food and health products. Only by controlling the packaging materials and environmental conditions can the requirements for the stability of the number of viable bacteria in the product be further met, and In the process of oral administration, it is easily interfered by the stress of digestive tract, gastric juice, bile salt, etc., and it is easy to lose its activity before entering the small intestine, and lose its proper probiotic effect, resulting in the actual release of the effective dose in the host intestinal tract failing to meet the requirements, so it is necessary to Conduct research in the direction of product dosage forms and preparation processes, and explore the form of probiotic products that can resist the stress of host gastric juice and bile salts and perform intestinal controlled release

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