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Microbial compound bacterial agent and preparation method and application thereof

A technology of compound bacterial agents and microorganisms, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low number of viable bacteria, weak ability to inhibit pathogenic bacteria, and affect utility

Inactive Publication Date: 2019-04-19
谭瑛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Probiotics are beneficial to the human body and have the effect of regulating intestinal health, but the existing probiotics generally have the following defects: 1. The ability to inhibit pathogenic bacteria is weak
2. The freeze-drying process of freeze-dried bacteria powder is directly related to the survival rate of the freeze-dried bacteria powder. After it is made into freeze-dried bacteria powder, the number of live bacteria is low, which affects its effectiveness

Method used

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  • Microbial compound bacterial agent and preparation method and application thereof
  • Microbial compound bacterial agent and preparation method and application thereof
  • Microbial compound bacterial agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The isolation and screening of embodiment 1 bacterial strain

[0041] The microbial complex bacteria agent of the present embodiment includes Lactobacillus brevis, Bifidobacterium longum, Saccharomyces bayanus and Streptococcus faecalis. The Lactobacillus brevis was submitted to the General Microorganism Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC) for preservation on July 17, 2018, and its preservation number was CGMCC NO. 16125; the Bifidobacterium longum was deposited on July 17, 2018 It was submitted to the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures on July 17, 2018 for preservation, and its preservation number was CGMCCNO. 16126; CGMCC) is preserved, and its preservation number is CGMCC NO.16127; The said faecal streptococcus was submitted to the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee on July 17, 2018...

Embodiment 2

[0068] The preparation of embodiment 2 microbial composite bacterial agents

[0069] The microbial composite bacterial agent of the present embodiment comprises Lactobacillus brevis, Bifidobacterium longum, Saccharomyces bayanus and Streptococcus faecalis described in embodiment 1.

[0070] Its preparation method is as follows:

[0071] A, prepare Lactobacillus brevis freeze-dried powder;

[0072] (1) Get the described Lactobacillus brevis and inoculate it into a 50L fermenter equipped with 35L of LYT fermentation medium according to an inoculation amount of 1%. Under the condition of Mpa, ferment 18h, obtain primary fermentation liquid in logarithmic growth phase; Then, get described primary fermentation liquid, inoculate in the fermenter that LYT fermentation medium is housed according to the inoculum size of 8%, at temperature Under the conditions of 37°C, rotating speed of 150rpm, and tank pressure of 0.05Mpa, anaerobic culture was carried out for 20 hours to obtain a se...

Embodiment 3

[0093] The preparation of embodiment 3 microbial composite bacterial agents

[0094] The microbial composite bacterial agent of the present embodiment includes Lactobacillus brevis, Bifidobacterium longum, Saccharomyces bayanus and Streptococcus faecalis described in embodiment 1. Its preparation method is as follows:

[0095] The preparation method of described microbial composite bacterial agent specifically comprises the steps:

[0096] (1) Take the Lactobacillus brevis, the Bifidobacterium longum, the Saccharomyces shellfish and the Streptococcus faecalis and ferment and culture them respectively to obtain the fermentation liquid of the Lactobacillus brevis and the Bifidobacterium longum. Fermented liquid, the fermented liquid of Saccharomyces bayi, the fermented liquid of Streptococcus faecalis;

[0097] (2) Take four kinds of fermented liquids obtained by fermentation in step (1), respectively place them at a rotating speed of 8000-12000 r / min, centrifuge for 10-30min,...

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Abstract

The invention provides a microbial compound bacterial agent. The microbial compound bacterial agent comprises lactobacillus brevis, bifidobacterium longum, saccharomyces bayanus and streptococcus faecium. The microbial compound bacterial agent has the advantages that the ability to inhibit pathogenic bacteria is strong, and the survival rate is high after the microbial compound bacterial agent isprepared into freeze-dried powder; four strains of the microbial compound bacterial agent have no mutual antagonistic effects and can be mutually-beneficial and symbiotic, the bifidobacterium longum,lactic acid bacillus and the streptococcus faecium all have strong antagonistic effects on the pathogenic bacteria, and the microbial compound bacterial agent formed by the four strains has good functions of regulating the gastrointestinal health and improving immunity.

Description

technical field [0001] The invention belongs to the technical field of human microecology, and in particular relates to a microbial compound bacterial agent and its preparation method and application. Background technique [0002] Various microorganisms (bacteria) often attach to the human body from different environments, and can settle in certain parts and continuously grow and reproduce. This phenomenon is usually called "bacterial colonization". Colonized microorganisms must rely on the continuous supply of nutrients from the human body to grow and reproduce, and then to have an impact on the human body. [0003] Generally speaking, the human body has the following immune mechanisms: [0004] a. Structural barrier: The "structural barrier" for young children to resist foreign pathogenic microorganism infection includes body surface barrier and internal barrier. The body surface barrier is composed of the skin covering the body surface and the mucous membrane lining the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A23L33/135A23C9/127A61K36/064A61P1/00A61P37/04A61P1/12A61K35/747A61K35/745A61K35/744C12R1/24C12R1/01C12R1/46
CPCA61P1/00A61P1/12A61P37/04A23C9/127A23L33/135A61K35/744A61K35/745A61K35/747A61K36/064C12N1/16C12N1/20A23V2002/00C12R2001/24C12N1/205C12R2001/46C12R2001/01A23V2400/121A23V2400/533A23V2200/32A23V2200/30A61K2300/00
Inventor 谭瑛
Owner 谭瑛
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