Bifidobacterium longum and application thereof

A technology of Bifidobacterium longum and bacteria slime, applied in the direction of Bifidobacterium longum, application, bacteria, etc., can solve the problem of low survival rate of Bifidobacterium longum bacteria powder, achieve good acid and bile salt resistance, inhibit invasion, strains Active ideal effect

Active Publication Date: 2019-03-19
谭瑛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The second technical problem to be solved by the present invention is the problem that the survival rate of the Bifidobacterium longum bacterial powder obtained by the freeze-dried bacterial powder process in the prior art is low, and then provides a frozen Bifidobacterium longum with a high survival rate of bacterial strains. Preparation method of dry powder

Method used

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  • Bifidobacterium longum and application thereof
  • Bifidobacterium longum and application thereof
  • Bifidobacterium longum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The isolation and screening of embodiment 1 Bifidobacterium longum

[0044] More than 30 types of Bifidobacteria were isolated from the feces of healthy newborn and weaned infants, and a Bifidobacteria microflora matching that of adults was found in non-allergic children (or more than normal children were found Higher and more types of bifidobacteria) These bifidobacteria adhere to the mucus in children's intestines and have a protective effect on children's bodies from toxic substances. Therefore, we can increase the content of bifidobacteria in children's food to Increases the resistance of children to diseases and allergic reactions to allergic substances.

[0045] The Bifidobacterium longum whose preservation number is CGMCC No.16126 of the present invention is isolated from the feces of newborns and infants, and is obtained by the following method:

[0046] Put 1 gram of human intestinal feces into a sterilized bottle containing 30 glass balls and add physiologica...

Embodiment 2

[0057] The preparation of embodiment 2 Bifidobacterium longum freeze-dried powder

[0058] The Bifidobacterium longum of this embodiment is a bacterial strain whose preservation number is CGMCC No.16126, and it is prepared as Bifidobacterium longum freeze-dried powder by the following method:

[0059] (1) Take the Bifidobacterium longum and culture it in coagulated milk with an initial pH value of 7.5 at a temperature of 37° C. for 36 to 48 hours. The inoculum amount of % is inoculated on coagulated milk as a medium, and cultured in a shaking flask culture; the coagulated milk can be commercially available coagulated milk, or can be prepared by itself using milk. For the realization of the purpose of the present invention, the culture medium used is not unique, and those skilled in the art can also replace the coagulated milk with other conventional bifidobacteria culture medium.

[0060] (2) Get the fermented liquid obtained by fermentation in step (1), place it in a centrif...

Embodiment 3

[0062] The preparation of embodiment 3 Bifidobacterium longum freeze-dried powder

[0063] The Bifidobacterium longum of this embodiment is a bacterial strain whose preservation number is CGMCC No.16126, and it is prepared as Bifidobacterium longum freeze-dried powder by the following method:

[0064] Add a protective agent to the Bifidobacterium longum, mix well, place it at -30°C for 1 hour, then place it at -20°C for sublimation for 10 hours, then place it at -10°C sublimation under low temperature for 20 hours, then secondary sublimation at 30°C for 5 hours, and place under vacuum for 1 hour to obtain the freeze-dried powder of Bifidobacterium longum.

[0065] In this embodiment, the protective agent is skimmed milk powder. As an alternative implementation of this embodiment, the protective agent can also be replaced by skimmed milk powder, maltose, sucrose, soluble starch, mannitol, sodium glutamate, L - one or more of cysteine, gelatin.

[0066] Effect test example

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Abstract

The invention provides bifidobacterium longum, which is submitted to CGMCC for storage in July 17, 2018, with the preservation number of CGMCC No.16126. The bifidobacterium longum provided by the invention is strong in pathogenic bacteria inhibiting capability and high in survival rate after being prepared into freeze-dried powder.

Description

technical field [0001] The invention belongs to the technical field of human microecology, and in particular relates to a bifidobacterium longum and its application. Background technique [0002] Various microorganisms (bacteria) often attach to the human body from different environments, and can settle in a certain part and continue to grow and reproduce. This phenomenon is usually called "bacterial colonization". Colonized microorganisms must rely on the continuous supply of nutrients from the human body to grow and reproduce, and then to have an impact on the human body. [0003] Generally speaking, the human body has the following immune mechanisms: [0004] a. Structural barrier [0005] The "structural barrier" for young children to resist foreign pathogenic microorganism infection includes body surface barrier and internal barrier. The body surface barrier is composed of the skin covering the body surface and the mucous membrane lining the cavity communicating with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23L33/135A23C9/12A61K35/745A61P31/04A61P1/12C12R1/01
CPCA61P1/12A61P31/04A23C9/1203A23L33/135C12N1/04C12N1/20A23V2002/00A61K2035/115C12R2001/01C12N1/205A23V2400/533A23V2200/324A23V2200/3204
Inventor 谭瑛
Owner 谭瑛
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