Composition based on lactobacillus plantarum and preparation method of composition

Abstract

The invention relates to the field of foods, and discloses a composition based on lactobacillus plantarum and a preparation method of the composition. The composition comprises lactobacillus plantarumfreeze-dried powder, white kidney bean extract, inulin, erythritol and vitamin C. The composition is capable of reducing body weight, reducing fat accumulation in the body, reducing the organ ratio and the body fat ratio, reducing the leptin level and reducing blood fat, and can be used for preventing and treatment obesity. The lactobacillus plantarum 1701 used for preparing the lactobacillus plantarum freeze-dried powder has good tolerance and adhesiveness, can significantly reduce body weight, reduce fat accumulation in the body, reduce the organ ratio and the body fat ratio and reduce theleptin level and blood fat, and can be used for preventing and treatment obesity; and in addition, after being inactivated, the lactobacillus plantarum 1701 also has the function of losing weight, sothat the stability and the shelf life of a product are longer.

Description

technical field

[0001] The invention relates to the field of food, in particular to a composition based on Lactobacillus plantarum and a preparation method thereof. Background technique

[0002] Obesity is mainly due to the disorder of the body's energy metabolism balance, resulting in energy intake exceeding energy consumption, resulting in excessive accumulation of body fat, resulting in metabolic diseases. Obesity is not only affected by multiple factors such as genetics, environment, metabolism, and physiology, but is also associated with diseases such as hyperlipidemia, hypertension, diabetes, insulin resistance, hyperuricemia, non-alcoholic fatty liver, and coronary atherosclerotic heart disease. Closely related. At present, obesity and its complications have become a major public health problem worldwide, which not only affects the appearance of individuals, but also poses serious hazards to human health.

[0003] At present, a large number of studies have shown tha...

Examples

preparation example Construction

[0080] The preparation method of described plantarum lactobacillus freeze-dried powder comprises the following steps:

[0081] 1) Preparation of medium: the medium is an improved MRS medium, and its formula is glucose 20-30g, beef extract 10-13g, tryptone 5-7g, soybean peptone 5-7g, yeast powder 5-6g, acetic acid Sodium 3-5g, diammonium hydrogen citrate 1-2g, dipotassium hydrogen phosphate 2-3g, magnesium sulfate 0.4-0.6g, cysteine ​​hydrochloride 0.4-0.7g, Tween-80 1-2mL, Manganese sulfate hydrate 0.2-0.25g, water 1000mL; adjust its pH to 6.5±0.2;

[0082] 2) Preparation of bacterial strain protective agent: the formula of the bacterial strain protective agent is skim milk 80g / L, trehalose 100g / L, glycerin 20g / L;

[0083] 3) Inoculate Lactobacillus plantarum and / or its mutants in the fermentation substrate with 5%-10% inoculation amount to carry out fermentation culture, the fermentation temperature is 34-38°C, the fermentation time is 13-18h, and the pH of the fermentation pr...

Embodiment 1

[0097] The bacterial strain provided by the present invention is identified as belonging to Lactobacillus plantarum (Lactobacillus plantarum), named 1701, and was preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee on October 23, 2019, and the microorganism preservation number is CGMCC No. .18728.

[0098] The bacterial strain provided by the present invention is isolated by the inventor from yogurt powder sample collected in the village of Shigatse City, Tibet Autonomous Region of my country.

[0099] The biological properties of bacterial strain Lactobacillus plantarum 1701 of the present invention are as follows:

[0100] Morphological characteristics: In MRS agar medium, the growth form is milky white, opaque, round, smooth and moist surface, neat edges, and centrally raised colonies. Gram staining was typically positive, and the cells were observed under a microscope to be long rod-shaped, without...

Embodiment 2

[0108] Lactobacillus plantarum 1701 of the present invention, contrast commercial bacterial strain Lactobacillus rhamnosus GG (LGG) and Lactobacillus casei Daita strain (LcS) after second-generation activation, get the bacterium liquid at the end of logarithmic growth, centrifuge at 4000rpm for 10min, discard the supernatant, To obtain the bacteria sludge, perform the following operations respectively: ①Add the same volume of MRS solution with pH 2.5, blow and mix well, incubate at 37°C, and measure the number of bacteria after incubation for 0h, 1h, 2h, and 4h by the dilution coating counting method ② Add the same volume of MRS solution containing 0.3% bile salts, pipette and mix well, incubate at 37°C, and measure the number of bacteria after incubation for 0h, 4h, and 8h by the dilution coating counting method. Strain survival rate calculation formula:

[0109] Strain survival rate (%)=N1 / N0×100%.

[0110] N1 is the number of viable bacteria after the strain was incubated,...

technical field

[0001] The invention relates to the field of food, in particular to a composition based on Lactobacillus plantarum and a preparation method thereof. Background technique

[0002] Obesity is mainly due to the disorder of the body's energy metabolism balance, resulting in energy intake exceeding energy consumption, resulting in excessive accumulation of body fat, resulting in metabolic diseases. Obesity is not only affected by multiple factors such as genetics, environment, metabolism, and physiology, but is also associated with diseases such as hyperlipidemia, hypertension, diabetes, insulin resistance, hyperuricemia, non-alcoholic fatty liver, and coronary atherosclerotic heart disease. Closely related. At present, obesity and its complications have become a major public health problem worldwide, which not only affects the appearance of individuals, but also poses serious hazards to human health.

[0003] At present, a large number of studies have shown tha...

Examples

preparation example Construction

[0080] The preparation method of described plantarum lactobacillus freeze-dried powder comprises the following steps:

[0081] 1) Preparation of medium: the medium is an improved MRS medium, and its formula is glucose 20-30g, beef extract 10-13g, tryptone 5-7g, soybean peptone 5-7g, yeast powder 5-6g, acetic acid Sodium 3-5g, diammonium hydrogen citrate 1-2g, dipotassium hydrogen phosphate 2-3g, magnesium sulfate 0.4-0.6g, cysteine ​​hydrochloride 0.4-0.7g, Tween-80 1-2mL, Manganese sulfate hydrate 0.2-0.25g, water 1000mL; adjust its pH to 6.5±0.2;

[0082] 2) Preparation of bacterial strain protective agent: the formula of the bacterial strain protective agent is skim milk 80g / L, trehalose 100g / L, glycerin 20g / L;

[0083] 3) Inoculate Lactobacillus plantarum and / or its mutants in the fermentation substrate with 5%-10% inoculation amount to carry out fermentation culture, the fermentation temperature is 34-38°C, the fermentation time is 13-18h, and the pH of the fermentation pr...

Embodiment 1

[0097] The bacterial strain provided by the present invention is identified as belonging to Lactobacillus plantarum (Lactobacillus plantarum), named 1701, and was preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee on October 23, 2019, and the microorganism preservation number is CGMCC No. .18728.

[0098] The bacterial strain provided by the present invention is isolated by the inventor from yogurt powder sample collected in the village of Shigatse City, Tibet Autonomous Region of my country.

[0099] The biological properties of bacterial strain Lactobacillus plantarum 1701 of the present invention are as follows:

[0100] Morphological characteristics: In MRS agar medium, the growth form is milky white, opaque, round, smooth and moist surface, neat edges, and centrally raised colonies. Gram staining was typically positive, and the cells were observed under a microscope to be long rod-shaped, without...

Embodiment 2

[0108] Lactobacillus plantarum 1701 of the present invention, contrast commercial bacterial strain Lactobacillus rhamnosus GG (LGG) and Lactobacillus casei Daita strain (LcS) after second-generation activation, get the bacterium liquid at the end of logarithmic growth, centrifuge at 4000rpm for 10min, discard the supernatant, To obtain the bacteria sludge, perform the following operations respectively: ①Add the same volume of MRS solution with pH 2.5, blow and mix well, incubate at 37°C, and measure the number of bacteria after incubation for 0h, 1h, 2h, and 4h by the dilution coating counting method ② Add the same volume of MRS solution containing 0.3% bile salts, pipette and mix well, incubate at 37°C, and measure the number of bacteria after incubation for 0h, 4h, and 8h by the dilution coating counting method. Strain survival rate calculation formula:

[0109] Strain survival rate (%)=N1 / N0×100%.

[0110] N1 is the number of viable bacteria after the strain was incubated,...

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