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High-density solid fermentation method for agricultural bacillus

A technology of bacillus and solid fermentation, which is applied in the field of high-density solid fermentation of agricultural bacillus, can solve the problems of immature solutions for solid fermentation methods and product marketization, and achieves high-value utilization and high content of viable bacteria. , the effect of easy assembly

Inactive Publication Date: 2016-05-11
JINAN HANGCHEN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although various manufacturers are actively exploring the solid fermentation technology of Bacillus in recent years; but so far, the traditional solid fermentation method has not There are mature solutions and products on the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A high-density solid fermentation method of an agricultural bacillus bacterial agent, comprising the steps of:

[0047] (1) Sterilization of fermented materials: the method is damp heat sterilization at a temperature of 121°C for 4 hours;

[0048] The composition of above-mentioned fermented material comprises:

[0049] 4 parts of quick-acting carbon sources: 3 parts of cornmeal, 1 part of peanut meal;

[0050] 8 parts of long-term carbon sources: specifically mushroom residue 3, furfural residue 2, chicken manure 2, rice husk 1;

[0051] 3 parts of nitrogen source: specifically corn steep liquor 1, yeast powder 1;

[0052] Dipotassium hydrogen phosphate 0.25%

[0053] Potassium dihydrogen phosphate 0.25% Magnesium sulfate 0.1%

[0054] Manganese sulfate 0.008% water 50%

[0055] Adjust the pH of the material to 7.0;

[0056] After the sterilization is completed, take out the material and cool down to about 40°C.

[0057](2) UV sterilize the self-made solid ferme...

Embodiment 2

[0065] A method for in-situ solid fermentation of an agricultural bacillus bacterial agent, comprising the steps of:

[0066] (1) Sterilization of fermented materials: the method is damp heat sterilization at 121°C for 3.5 hours;

[0067] The composition of above-mentioned fermented material comprises:

[0068] The available carbon source is 3.5 parts soybean meal

[0069] The long-term carbon source is 8 parts, specifically furfural slag and cotton firewood with a weight ratio of 2:1;

[0070] 4 parts of nitrogen source, specifically corn steep liquor: potassium nitrate with a weight ratio of 3:1;

[0071] Dipotassium hydrogen phosphate 0.5%

[0072] Potassium dihydrogen phosphate 0.5% Magnesium sulfate 0.08%

[0073] Manganese sulfate 0.009% water 55%

[0074] Adjust the pH of the material to 6.8-7.2;

[0075] After the sterilization is completed, the materials are taken out and the temperature is lowered to 37°C.

[0076] (2) UV sterilize the self-made solid fermenta...

Embodiment 3

[0082] A method for in-situ solid fermentation of an agricultural bacillus bacterial agent, comprising the steps of:

[0083] (1) Sterilization of fermented materials: the method is damp heat sterilization at 121°C for 2 hours;

[0084] The composition of above-mentioned fermented material comprises:

[0085] 2 parts of quick-acting carbon source, specifically 1.5 parts of glucose and 0.5 parts of glucose;

[0086] 1.5 parts of long-term carbon source, specifically 0.5 parts of mushroom dregs, 1 part of cotton shell;

[0087] 1 part of nitrogen source, specifically 0.5 part of peptone, 0.5 part of peanut cake powder;

[0088] Dipotassium hydrogen phosphate 0.1%

[0089] Potassium dihydrogen phosphate 0.1% Magnesium sulfate 0.05%

[0090] Manganese sulfate 0.006% water 40%

[0091] Adjust the pH value of the material to 6.8;

[0092] After the sterilization is completed, the materials are taken out and the temperature is lowered to 30°C.

[0093] (2) UV sterilize the sel...

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Abstract

The invention belongs to the technical field of microorganism bacterium agent fermentation, and particularly relates to a high-density solid fermentation method for agricultural bacillus. The fermentation method comprises the following steps that 1, sterilization treatment is performed on fermentation materials; 2, a fermentation device is sterilized; 3, the fermentation materials in the step 1 are put into the fermentation device in the step 2, and bacillus is inoculated into the fermentation materials; 4, aerated fermentation is performed on the fermentation materials; 5, the fermentation materials are dried. According to the method, the raw materials of a culture medium are simple, choosing range is wide, and the cost is low; the floor space is small, the requirement on equipment is low, special machines and tools are not needed, assembly is convenient, energy consumption is low, and therefore the production cost is much lower than that of liquid fermentation and only accounts for 30%-50% of that of liquid fermentation; compared with pile fermentation and traditional shallow tray fermentation, the fermentation method has the advantages that the requirements for temperature, humidity and ventilatory capacity in the bacillus growth process are taken into account, and the problems such as metabolic heat removal and mass transfer in the bacillus solid fermentation process are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of microbial agent fermentation, and in particular relates to a high-density solid fermentation method of agricultural bacillus. Background technique [0002] Bacillus are a class of aerobic and facultatively anaerobic, Gram-positive rod-shaped bacteria that produce stress-resistant endospores. The fungus is widely distributed in nature, easy to isolate and cultivate, harmless to humans and animals, and friendly to the environment. Bacillus grows fast, has simple nutrition, and can produce heat-resistant and stress-resistant spores. The spores have strong stress resistance and are conducive to preservation. The damage caused by , ultraviolet rays, etc., this biological feature makes it an ideal agricultural microorganism. After the bacillus bacteria agent is applied to the farmland, it can fully decompose the insoluble macromolecular components in the soil and convert them into small molecular water-solu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C12M1/16C12M1/12C12R1/07
CPCC12P1/04C12M21/16C12M37/00
Inventor 赵林李昆仑刘金钊高海燕
Owner JINAN HANGCHEN BIOTECHNOLOGY CO LTD
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