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Lactobacillus plantarum and application of lactobacillus plantarum in preparation of medicines for treating infectious vaginosis

A technology of Lactobacillus plantarum and vaginal pathogenic bacteria, which is applied in the field of microorganisms, can solve the problems of poor antibacterial effect, no patent publications have been found, gastrointestinal intolerance, etc., and achieve good antibacterial ability and good acid production Effect of hydrogen peroxide capacity, good acid resistance and bile salt tolerance

Active Publication Date: 2021-11-26
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although a variety of Lactobacillus plantarum with antioxidant activity have been screened out, most of them have disadvantages such as poor antibacterial effect or gastrointestinal intolerance.
[0006] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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  • Lactobacillus plantarum and application of lactobacillus plantarum in preparation of medicines for treating infectious vaginosis
  • Lactobacillus plantarum and application of lactobacillus plantarum in preparation of medicines for treating infectious vaginosis
  • Lactobacillus plantarum and application of lactobacillus plantarum in preparation of medicines for treating infectious vaginosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Screening and identification of strains

[0047] (1) Isolation and purification of bacterial strains

[0048] The Lactobacillus plantarum 021 was screened in mung bean physalis produced in Henan area. The mung bean milk solution was diluted with sterile PBS buffer solution and spread on the MRS plate medium, cultured at 37°C for 48 hours under anaerobic conditions, and well-separated single colonies were picked and streaked. A single colony was picked by streaking multiple times, and the purified strain was stained with Gram staining, and the morphology and distribution of the bacteria were observed under a microscope. This strain is a Gram-positive round coccus, see figure 1 .

[0049] The bacterial solution was diluted 10 times with sterile saline to 10 -6 , take 10 -4 、10 -5 、10 -6100 μl of the three gradient dilutions were spread on the MRS broth solid plate, cultured at 37°C for 48 hours, and colonies of different shapes were selected for strea...

Embodiment 2

[0054] Embodiment 2: Lactobacillus plantarum 021 acid tolerance

[0055] (1) After activating the lactic acid bacteria for 2-3 times, use 1.0X10 9 The concentration of CFU / mL was respectively inoculated in MRS broth medium with pH 2, 3 and 4 according to 1% inoculum volume and cultured for 4 hours.

[0056] (2) After the culture is over, use the normal MRS broth medium as a control, mix the bacterial solution thoroughly, add it to a 96-well plate that has been sterilized in advance, 100 μL per well, set 3-6 duplicate wells, and use multiple The functional microplate reader measures the absorbance at 600nm to detect the inhibition rate of the culture medium with different pH values ​​on lactic acid bacteria.

[0057] Formula: survival rate (%)=(treatment group / control group) X 100%

[0058] The result is as figure 2 Shown: Through experiments, it was found that the survival rate of Lactobacillus plantarum 021 in the MRS broth medium with pH 2 and pH 3 is relatively high, wh...

Embodiment 3

[0059] Embodiment 3: Lactobacillus plantarum 021 tolerance to bile salts

[0060] (1) After activating the lactic acid bacteria for 2-3 times, use 1.0X10 9 The concentration of CFU / mL was respectively inoculated in MRS broth medium containing 0.1%, 0.2%, and 0.3% porcine bile salt according to the inoculum size of 1%, and cultured for 4 hours.

[0061] (2) After the culture is over, use the normal MRS medium as a control, mix the bacterial solution thoroughly, add it to a 96-well plate that has been sterilized in advance, 100 μL per well, set 3-6 duplicate wells, and use a multifunctional enzyme The absorbance at 600nm was measured by a standard instrument to detect the inhibition rate of different contents of bile salts on lactic acid bacteria.

[0062] Formula: survival rate (%)=(treatment group / control group) X 100%

[0063] The result is as image 3 Shown: Through experiments, it is found that Lactobacillus plantarum 021 can grow in MRS broth medium containing different...

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Abstract

The present invention relates to lactobacillus plantarum. The name of the lactobacillus plantarum is lactobacillus plantarum 021, the classification name of the lactobacillus plantarum is lactobacillus plantarum, the preservation number of the lactobacillus plantarum is CGMCC No.22169, the preservation date of the lactobacillus plantarum is April 12, 2021, and the preservation unit of the lactobacillus plantarum is China General Microbiological Culture Collection Center, Institute of Biology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing. The lactobacillus plantarum disclosed by the invention has better acid-resistant, cholate-resistant, acid-producing and hydrogen peroxide-producing capabilities. The lactobacillus plantarum has a good adhesion effect on cervical epithelial cells, and meanwhile has a good capability of inhibiting candida albicans and staphylococcus aureus and high antibiotic anti-sensitivity. In addition, the lactobacillus plantarum has an inhibiting effect on candida albicans infectious vaginosis.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to plant lactobacillus and its application, in particular to plant lactobacillus and its application in the preparation of infectious vaginosis treatment medicine. Background technique [0002] Lactobacillus is currently recognized as the most important probiotic member in the normal vaginal flora, especially the ability to produce H 2 o 2 Lactobacillus, by synthesizing H 2 o 2 , acting directly or in combination with other peroxides and halides to inhibit the growth of other bacteria. As the dominant flora of the vagina, they can decompose the glycogen in the epithelium of the vaginal mucosa to produce a certain amount of lactic acid, so that the vagina can maintain an acidic environment, which is beneficial to the self-purification of the vagina. At the same time, the lactobacilli in the vaginal microecology can improve the immune function of the body, activate macrophages, mai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P31/10A61P15/02C12R1/25
CPCA61K35/747A61P31/04A61P31/10A61P15/02Y02A50/30
Inventor 何红鹏罗学刚杨雅萍王傲扈士月赵阳张同存
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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