Lactobacillus plantarum n3117, its screening method and application
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A technology of Lactobacillus plantarum and microbial strains, applied in the field of microbial engineering, can solve problems such as regulating immune function and losing weight without Lactobacillus plantarum
Active Publication Date: 2019-04-16
JUNLEBAO DAIRY GRP CO LTD
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However, there is no report on the regulation of immune function by Lactobacillus plantarum to achieve weight loss
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Embodiment 1
[0049] Embodiment 1 A kind of Lactobacillus plantarum N3117 and screening method thereof
[0050] A kind of Lactobacillus plantarum (Lactobacillus plantarum) N3117, which is an acid-resistant and bile-resistant probiotic isolated from fermented milk products in Inner Mongolia; , deposit number: CGMCC NO.10133.
[0051] The screening method of Lactobacillus plantarum N3117 is carried out according to the following steps:
[0052] (1) Source of fermented yogurt
[0053] The selected fermented yoghurt comes from the traditional fermented milk products of Inner Mongolia;
[0054] (2) Enrichment of samples
[0055] Take 2 mL of the sample, add it to 100 mL of MRS liquid medium, and incubate at 35°C for 72 hours to obtain a culture solution;
[0056] (3) Isolation and purification of strains
[0057] a. Take 1 mL of the culture solution and dilute it 100000 times with 0.9% sterile normal saline by weight to volume, and then serially dilute 10 times -1 , 10 -2 , 10 -3 , 10 -...
Embodiment 2
[0104] Embodiment 2 The preparation of the lactic acid bacteria drink containing plantaractobacillus N3117
[0105] Mix 48g of pure milk, 32g of water, and 5g of sucrose, and sterilize at 110°C for 15 minutes to prepare a culture medium; 6 Lactobacillus plantarum N3117, 10 cfu / ml 6 cfu / ml of Lactobacillus bulgaricus and 10 6 Streptococcus thermophilus of cfu / ml is inserted into the medium and mixed evenly, fermented at 37°C until the pH reaches 4.4-4.6, the fermentation is terminated, cooled and filled, and refrigerated. The product is within the 30d shelf life. There will be no significant change, and the number of bacteria will remain at 10 6 cfu / ml did not change significantly.
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Abstract
The invention discloses Lactobacillus plantarum N3117, which is an acid-resistant and bile-salt-resistant probiotic isolated from fermented milk products in Inner Mongolia; , Preservation number: CGMCC NO.10133; The screened Lactobacillus plantarum N3117 was successively subjected to experiments such as sample enrichment, separation, and purification, and finally the Lactobacillus plantarum N3117 was screened out. The screened Lactobacillus plantarum N3117 can be used to prepare yoghurt, lactic acid bacteria drinks and bacterial powder preparations containing Lactobacillus plantarum N3117. The invention is suitable for the screening of plantaractobacillus N3117 and the preparation of functional milk products containing plantaractobacillus N3117 and probiotic bacteria powder.
Description
technical field [0001] The invention belongs to the field of microbial engineering, and relates to a plant lactobacillus, specifically a plant lactobacillus (Lactobacillus plantarum) N3117, a screening method and application thereof. Background technique [0002] Lactobacillus is one of the main beneficial bacteria in the normal intestinal flora of animals. It is an important health-promoting factor for animals. It can regulate the balance of intestinal flora, improve immunity, relieve lactose intolerance and inhibit the formation of tumor cells. , Promote digestion and absorption of nutrients, lower cholesterol levels, etc. However, the premise of its physiological function is to be able to tolerate the acidic and high bile salt environment of the upper digestive tract, and to have good adhesion properties. [0003] Lactobacillus plantarum is a probiotic group in the human gastrointestinal tract. Its metabolism can produce organic acids, bacteriocins, hydrogen peroxide, di...
Claims
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