High-activity lactobacillus plantarum N3117 as well as screening method and application thereof

A high-activity technology of Lactobacillus plantarum, applied in the field of microbial engineering, can solve problems such as regulating immune function and losing weight without Lactobacillus plantarum

Active Publication Date: 2016-03-23
JUNLEBAO DAIRY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the regulation of immune function by Lactobacillus plantarum to achieve weight loss

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 A kind of Lactobacillus plantarum N3117 and screening method thereof

[0050] A kind of Lactobacillus plantarum (Lactobacillus plantarum) N3117, which is an acid-resistant and bile-resistant probiotic isolated from fermented milk products in Inner Mongolia; Deposit number: CGMCCNO.10133.

[0051] The screening method of Lactobacillus plantarum N3117 is carried out according to the following steps:

[0052] (1) Source of fermented yogurt

[0053] The selected fermented yoghurt comes from the traditional fermented milk products in Inner Mongolia;

[0054] (2) Enrichment of samples

[0055] Take 2 mL of the sample, add it to 100 mL of MRS liquid medium, and incubate at 35°C for 72 hours to obtain a culture solution;

[0056] (3) Isolation and purification of strains

[0057] a. Take 1 mL of the culture medium and dilute it 100,000 times with 0.9% weight-to-volume sterile normal saline, and serially dilute 10 times respectively. -1 , 10 -2 , 10 -3 , 10 ...

Embodiment 2

[0099] Embodiment 2 The preparation of the lactic acid bacteria drink containing plantaractobacillus N3117

[0100] Mix 48g of pure milk, 32g of water, and 5g of sucrose, and sterilize at 110°C for 15 minutes to prepare a culture medium;

[0101] will be 106 Lactobacillus plantarum N3117, 10 cfu / ml 6 cfu / ml of Lactobacillus bulgaricus and 10 6 Streptococcus thermophilus of cfu / ml is inserted into the medium and mixed evenly, fermented at 37°C until the pH reaches 4.4-4.6, the fermentation is terminated, cooled and filled, and refrigerated. The product is within the 30d shelf life. There will be no significant change, and the number of bacteria will remain at 10 6 cfu / ml did not change significantly.

Embodiment 3

[0102] Embodiment 3 Preparation of probiotic bacteria powder containing Lactobacillus plantarum N3117

[0103] Composition of raw materials: The product content per gram is: Lactobacillus casei 1×10 10 cfu / g, Lactobacillus plantarum N31171×10 10 cfu / g, Lactobacillus acidophilus 1×10 10 cfu / g, Bifidobacterium lactis 1×10 10 cfu / g, white sugar 0.5g, fructooligosaccharide (prebiotic) 0.3g, strawberry powder 0.1g, maltodextrin 0.12g;

[0104] The preparation process of the probiotic bacteria powder is as follows: Weigh the bacteria powder, white sugar, fructooligosaccharide, strawberry extract, and maltodextrin according to the formula ratio, mix them, and pack them on a vacuum packing machine, 2g / bag (including active lactic acid bacteria Total ≥ 4×10 10 cfu / bag), labeling and storage, refrigerated storage, during the shelf life of the product, the sensory and flavor of the product will not change significantly, and the number of bacteria will remain at 10 10 The cfu / bag did...

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PUM

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Abstract

The invention discloses lactobacillus plantarum N3117. The lactobacillus plantarum N3117 is acid-resistant and bile-salt-resistant probiotic separated from a fermented dairy product in Inner Mongolia; the strain of the lactobacillus plantarum N3117 is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.10133; the screened lactobacillus plantarum N3117 is sequentially subjected to tests including sample enrichment, separation, purification and the like, and the lactobacillus plantarum N3117 is screened out finally. The screened lactobacillus plantarum N3117 can be used for preparing yoghurt, sour milk beverages and probiotic powder preparations containing the lactobacillus plantarum N3117. The method is applicable to screening of the lactobacillus plantarum N3117 and the lactobacillus plantarum N3117 is applicable to preparation of functional dairy products and probiotic powder containing the lactobacillus plantarum N3117.

Description

technical field [0001] The invention belongs to the field of microbial engineering, and relates to a plant lactobacillus, specifically a highly active plant lactobacillus (Lactobacillus plantarum) N3117, a screening method and application thereof. Background technique [0002] Lactobacillus is one of the main beneficial bacteria in the normal intestinal flora of animals. It is an important health-promoting factor for animals. It can regulate the balance of intestinal flora, improve immunity, relieve lactose intolerance and inhibit the formation of tumor cells. , Promote digestion and absorption of nutrients, lower cholesterol levels, etc. However, the premise of its physiological function is to be able to tolerate the acidic and high bile salt environment of the upper digestive tract, and to have good adhesion properties. [0003] Lactobacillus plantarum is a probiotic group in the human gastrointestinal tract. Its metabolism can produce organic acids, bacteriocins, hydroge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A23C9/12A23L2/00A23L33/00C12R1/25
CPCA23C9/12A23L2/00C12N1/02A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/30
Inventor 魏立华朱宏王世杰冯丽莉王华张栋薛玉玲马蕊丛懿洁
Owner JUNLEBAO DAIRY GRP CO LTD
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