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Lactobacillus helveticus strain with depression improving effect and application of lactobacillus helveticus strain

A technology of Lactobacillus and strains, applied in the field of Lactobacillus helveticus, to achieve the effect of improving the level of BDNF, good barrier protection ability, and good adhesion characteristics

Active Publication Date: 2021-09-24
HANGZHOU WAHAHA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of drugs is prone to side effects, causing physical discomfort such as sweating, vomiting, tremors, and bipolar disorder

Method used

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  • Lactobacillus helveticus strain with depression improving effect and application of lactobacillus helveticus strain
  • Lactobacillus helveticus strain with depression improving effect and application of lactobacillus helveticus strain
  • Lactobacillus helveticus strain with depression improving effect and application of lactobacillus helveticus strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] The making of embodiment 1 Lactobacillus helveticus WHH1889 freeze-dried bacteria powder

[0099] The strain Lactobacillus helveticus WHH1889 of the present invention is inoculated in 10 ml of liquid MRS medium with an inoculum amount of 1%, cultivated in a constant temperature incubator at 37° C. for 8 hours (one-generation seed solution), and thus passed to two generations. Inoculate 1% of the second-generation seeds into a 10L fermenter containing liquid MRS medium, incubate at 37°C for 8 hours, collect the bacterial liquid, centrifuge at 8000rpm for 10 minutes to collect the bacterial cells, wash once with 0.9% normal saline, and add four times the bacterial sludge A certain amount of protective agent containing skimmed milk powder, glucose and glycerin was resuspended, vacuum freeze-dried, and bacterial powder was vacuum-packed. The number of viable bacteria in the prepared bacteria powder can reach 1×10 11 CFU / g, which can be used in the preparation and productio...

Embodiment 2

[0100] Example 2 Production of Lactobacillus helveticus WHH1889 fermented milk Accurately weigh 100 g of skim milk, 900 g of purified water at 45-50°C, dissolve in warm water at 50°C, shear for 20 minutes, hydrate at 50°C for 30 minutes, homogenize, and sterilize at 95°C for 5 minutes. After cooling down, insert the Lactobacillus helveticus WHH1889 starter in Application Example 1 according to the inoculation amount of 1%, ferment at 37° C. for 10 hours, and post-ripen at 4° C. for 12 hours. Observe the state of curdling, break the emulsion with an egg beater, test the drawing length, pH, acidity, viscosity and the number of viable bacteria, and set three repetitions. The results are shown in Table 6.

[0101] Table 6 Characteristics of Lactobacillus helveticus WHH1889 fermented milk

[0102] pH Acidity (°T) Viscosity (cp) Viable count (CFU / mL) 4.40±0.02 96±0.75 3289±9.21 1.20E+09

[0103] The fermented milk curd of Lactobacillus helveticus WHH1889 ...

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Abstract

The invention discloses lactobacillus helveticus with a depression improving function. The lactobacillus helveticus is named as WHH1889, is separated from milk residues collected from country of Shigatse city in Tibet Autonomous Region in China, is preserved in China General Microbiological Culture Collection Center on February 25, 2021, and has a preservation number of CGMCC NO.21832. The microbial classification is named as Lactobacillus helveticus. The lactobacillus helveticus strain has good tolerance, adhesiveness and intestinal barrier protection performance, can effectively relieve depression / anxiety-like behaviors of depression mice and callback the level of depressive nerve factors, is excellent in fermentation characteristic, can be applied to fermented milk and can also be used for drugs, health care products or healthy food for preventing and treating depression.

Description

technical field [0001] The invention relates to the technical field of depression improvement, in particular to a strain of Lactobacillus helveticus capable of improving depression and application thereof. Background technique [0002] Depression is a class of mood disorders characterized by depression and low mood. Depressed patients are often accompanied by other cognitive, physical and behavioral symptoms such as inattention, insomnia, unresponsiveness, decreased behavioral activities, and fatigue on the basis of low mood. According to the World Health Organization (WHO), it is estimated that more than 350 million people worldwide are suffering from depression, and the global average incidence rate is about 4.4%. In terms of gender, the average incidence rate of females is 5.1%, which is higher than 3.6% of males; in terms of age, the prevalence of depression in men aged 55-74 exceeds 5.5%, and the prevalence of depression in women aged 55-74 exceeds 7.5%, of which wome...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P25/24A61P25/22A23L33/00A23C9/123A23C9/152A23C19/00C12R1/225
CPCC12N1/20A61K35/747A61P25/24A61P25/22A23L33/00A23C9/1234A23C9/152A23C19/00A23V2002/00A23V2400/147A23V2200/30A23V2200/322
Inventor 高侃柯雪琴陈彩玲俞赟霞陈苏余腾斐李言郡朱珺
Owner HANGZHOU WAHAHA TECH
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