The invention provides an anti-alcohol probiotic fruit and vegetable freeze-dried powder and a preparation method thereof. The anti-alcohol probiotic fruit and vegetable freeze-dried powder is formed in a way that lactobacillus reuteri, lactobacillus plantarum, lactobacillus bulgaricus, lactobacillus casei, lactobacillus helveticus, lactobacillus rhamnosus and streptococcus thermophiles are mixed at a ratio, wherein the lactobacillus reuteri, the lactobacillus plantarum, the lactobacillus bulgaricus, the lactobacillus casei, the lactobacillus helveticus, the lactobacillus rhamnosus and the streptococcus thermophiles take Daucus carota var. sativa Hoffm., Lycopersicon esculentum, Brassica oleracea L. and soybean milk as raw materials, and the viable count of the lactobacillus reuteri, the lactobacillus plantarum, the lactobacillus bulgaricus, the lactobacillus casei, the lactobacillus helveticus, the lactobacillus rhamnosus and the streptococcus thermophiles is independently 10<7>-10<9> cfu/g. The anti-alcohol probiotic fruit and vegetable freeze-dried powder is obtained in a way that the pure natural Daucus carota var. sativa Hoffm., Lycopersicon esculentum, Brassica oleracea L. and soybean milk is fermented through probiotics, and therefore, the anti-alcohol probiotic fruit and vegetable freeze-dried powder does not contain any chemical synthesis substance, is a pure natural compound probiotic fruit and vegetable freeze-dried powder capable of efficiently degrading alcohol, and has the functions of boosting immunity, regulating intestinal flora balance, preventing constipation and diarrhea and the like.