The invention relates to the technical field of microbial culture and application, and discloses a high-density culture method of
lactobacillus helveticus, and preparation and application of high-activity bacterial
powder, aiming at the problems of
low activity and long culture period of
lactic acid bacteria in the prior art. The high-density culture method comprises the following steps of (1) inoculating a
lactobacillus helveticus strain into a seed culture medium for activation, performing culturing to obtain a seed solution; and (2) inoculating the seed solution into a
lactobacillus helveticus high-density
fermentation culture medium, and carrying out
fermentation culture at 35-43 DEG C under an anaerobic stirring condition to obtain the viable
bacteria fermentation liquor after the fermentation is finished. The fermentation liquor obtained by the method is applied to preparation of high-activity
lactobacillus helveticus powder. A high-density whey proliferation culture medium is provided, and
lactobacillus helveticus WHH2580 is subjected to high-density culture, so that a large number of thalli can be obtained. By adopting an optimized freeze-
drying protective agent, the
survival rate of thalli is greatly increased, the high-activity
lactobacillus helveticus powder is prepared, and the preservation period of the freeze-dried powder is prolonged.