Lactobacillus helveticus microcapsule, preparation and use thereof

A Lactobacillus and microcapsule technology, applied in the field of Lactobacillus helveticus microcapsules and their preparation, can solve problems such as poor resistance to deficit, poor resistance to bacteriophage infection, post-acidification of products, etc. The effect of efficiency

Inactive Publication Date: 2008-12-17
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0008] In order to overcome the problems of Lactobacillus helveticus's poor resistance to high temperature, acid and stress, and in the production and fermentation of Lactobacillus helveticus, the bacterium caused by free bacterial cells has poor ability to resist phage in

Method used

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  • Lactobacillus helveticus microcapsule, preparation and use thereof
  • Lactobacillus helveticus microcapsule, preparation and use thereof
  • Lactobacillus helveticus microcapsule, preparation and use thereof

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Embodiment 1

[0065] Preparation method of Lactobacillus helveticus microcapsules.

[0066] First prepare the bacteria sludge, which is about to be activated by skim milk Lactobacillus helveticus strains (Lactobacillus helveticus strains are purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC6024), by 4% inoculum size, inoculated Put into optimized 2000mL MRS proliferation medium, (MRS1600mL, tomato juice 150mL, wort juice 220mL, whey powder 30mL) utilize 20% sodium carbonate solution every 3h to adjust the pH value of fermented liquid to be 6.2, carry out proliferation culture 15h to thalline , to the early stage of the stable phase, then centrifuged at 8000rpm / min for 20min to collect the bacteria.

[0067] Add 200mL of thalline freeze-drying protective agent (10% skimmed milk 190mL, trehalose 3g, glycerin 1g, sorbitol 4g, maltodextrin 2g) in the bacterium slime that above-mentioned makes to prepare bacterium suspension, make t...

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Abstract

The invention provides a lactobacillus helveticus microcapsule as well as the preparation and application thereof, pertaining to the embedding technology of lactobacillus helveticus and aiming at microencapsulating the lactobacillus helveticus so as to solve the problems of low embedding efficiency and embedding yield in single-layer or double-layer embedment. The technical proposal adopted by the invention is that isolated soy protein, microporous starch and sodium alginate are respectively taken as the first, the second and the third layers of wall materials for microencapsulation; the three-layer embedment is carried out to the lactobacillus helveticus for the first time, wherein, in the process of preparing bacterial suspension, an orthogonal optimization protective agent is adopted to improve the livability after freeze drying. The microcapsule of the invention has strong heat resistance and acid resistance, solves the inactivation problem caused by gastric acid, digestive enzyme and antibiotic, etc. to active bacillus, is capable of releasing when reaching large intestine, and balances intestinal flora. The reusability of fermented milk is good. The lactobacillus helveticus used by the invention enriches the variety of probiotic bacteria microcapsule and is a good dietary supplement.

Description

field of invention [0001] The invention provides Lactobacillus helveticus microcapsules and its preparation and application, belongs to the field of biotechnology, and relates to Lactobacillus helveticus microcapsules and its preparation method and application. Background technique [0002] Probiotics are microbial supplements that exert beneficial effects on the host by improving the balance of intestinal microorganisms. They have a variety of physiological effects on the body that cannot be compared with other normal physiological flora. For example: improving intestinal function, resisting intestinal pathogenic bacteria, promoting lactose digestion, anti-cancer, anti-allergy, regulating immune system, lowering blood fat, lowering blood pressure, resisting urinary system infection, etc. [0003] Lactobacillus helveticus belongs to probiotics, which can be isolated from the human intestinal tract, yogurt, cheese-making primer or cheese. Lactobacillus helveticus has strong p...

Claims

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Application Information

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IPC IPC(8): C12N11/04A23C9/123A23L1/29A23L2/02A61P1/04A61P37/04A61P9/12A61P3/06C12R1/225A23L33/00
Inventor 王艳萍许女
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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