Lactobacillus helveticus capable of inhibiting helicobacter pylori and application of the lactobacillus helveticus

A technology of Helicobacter pylori and Lactobacillus, applied in the field of microbiology, can solve the problems of unsatisfactory treatment effect, reduced treatment efficiency, increased drug resistance of Helicobacter pylori, etc., and achieve the effect of reducing IL-8 level

Inactive Publication Date: 2020-07-31
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the frequent use of antibiotics in the above-mentioned treatment methods, the drug resistance of Helicobacter pylori is likely to increase, and in the process of using the above-mentioned treatment methods to treat patients, patients often have serious adverse reactions (such as abdominal pain, nausea, diarrhea, etc.), Leading to a reduction in the effective rate of treatment, the treatment effect is often not as expected

Method used

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  • Lactobacillus helveticus capable of inhibiting helicobacter pylori and application of the lactobacillus helveticus
  • Lactobacillus helveticus capable of inhibiting helicobacter pylori and application of the lactobacillus helveticus
  • Lactobacillus helveticus capable of inhibiting helicobacter pylori and application of the lactobacillus helveticus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1: Screening and identification of Lactobacillus helveticus

[0068] 1. Screening

[0069] Take fresh feces from healthy people in Kunshan City, Jiangsu Province as the sample. After the sample is pretreated, it is stored in a -80°C refrigerator in about 30% glycerol. Perform gradient dilution with 0.9% normal saline, select the appropriate gradient dilution and spread it on the MRS solid medium, incubate at 37°C for 48 hours, pick typical colonies to streak and purify on the MRS plate, pick a single colony and transfer it to liquid MRS The liquid medium was enriched and stored in 30% glycerol to obtain the strain CCFM1121 (the original serial number of the strain was M2-09-R02-S146).

[0070] 2. Identification

[0071] The genome of CCFM1121 was extracted, and the 16S rDNA of CCFM1121 was amplified and sequenced (completed by Shanghai Sangon Bioengineering Co., Ltd.). After sequencing analysis, the 16S rDNA sequence of the bacterial strain was shown in SEQ...

Embodiment 2

[0072] Embodiment 2: the cultivation of Lactobacillus helveticus

[0073] After inserting Lactobacillus helveticus CCFM1121 into the MRS solid medium and culturing at 37°C for 48 hours, the colonies were observed and observed under a microscope. It was found that the colonies were milky white semicircular protrusions with smooth and moist surfaces and edges tidy.

[0074] Lactobacillus helveticus CCFM1121 was inserted into MRS liquid medium and cultured at 37°C for 48 hours. During the cultivation process, the pH of the culture solution was measured by a pH meter at intervals. It was found that Lactobacillus helveticus CCFM1121 produced acid during the cultivation process.

[0075] Insert Lactobacillus helveticus CCFM1121 into the MRS liquid medium and culture at 10-50°C for 48 hours. During the cultivation process, measure the OD of the culture solution with a microplate reader at intervals 600 , it was found that Lactobacillus helveticus CCFM1121 grew best at 30-37°C, and r...

Embodiment 3

[0076] Example 3: Inhibition of Lactobacillus helveticus to Helicobacter pylori

[0077] Using the MRS liquid medium as a negative control, streak Lactobacillus helveticus CCFM1121 on the MRS solid medium, culture at 37°C for 48 hours, and obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, and incubate at 37°C Cultivate for 18 hours to activate, and activate two generations continuously to obtain the activation solution; inoculate the activation solution in the MRS liquid medium at an inoculum size of 2% (v / v), and cultivate it for 18 hours at 37°C to obtain the bacterial solution; After being centrifuged at 8000g for 10min, pass through a 0.22 μm sterile filter membrane to obtain the supernatant; use the Oxford cup method to measure the effect of the supernatant on inhibiting the growth of Helicobacter pylori, and the results are shown in Table 1 (the Oxford cup method can refer to the literature for details: Zhang Tingting, Zhai Qixiao, J...

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Abstract

The invention discloses lactobacillus helveticus capable of inhibiting helicobacter pylori and application of the lactobacillus helveticus, and belongs to the technical field of microorganisms and medicines. The invention provides a strain of lactobacillus helveticus CCFM1121, and the lactobacillus helveticus CCFM1121 can be used for inhibiting helicobacter pylori, specifically, the size of an inhibition zone of the lactobacillus helveticus CCFM1121 supernate on the helicobacter pylori can reach 10.00 mm, visually, the Lactobacillus helveticus CCFM1121 has a huge application prospect in the aspects of inhibiting Helicobacter pylori (not aiming at diagnosis and treatment of diseases) and preparing Helicobacter pylori inhibitors.

Description

technical field [0001] The invention relates to a strain of Lactobacillus helveticus capable of inhibiting Helicobacter pylori and an application thereof, belonging to the technical field of microorganisms and medicine. Background technique [0002] Helicobacter pylori (Hp) is a microaerophilic Gram-negative bacterium that colonizes the gastric mucosa and duodenum. Helicobacter pylori is helical or S-shaped, most of the Helicobacter pylori in the growth phase are rod-shaped or helical, and the Helicobacter pylori in the aging or dead state is spherical. [0003] Helicobacter pylori is one of the most important epidemic pathogens, and its detection rate in the global population has exceeded 50%. The infection rate of Helicobacter pylori is closely related to the health status, and children are generally more susceptible to infection. Helicobacter pylori can survive in the human body for a long time, and its incidence increases with age, and is related to socioeconomic statu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P31/04A61P1/04C12R1/225
CPCA23L33/135A61K35/747A61P31/04A61P1/04A23V2002/00C12R2001/225C12N1/205A23V2400/147A23V2200/32
Inventor 翟齐啸陈卫张美怡于雷雷田丰伟刘小鸣赵建新张灏
Owner JIANGNAN UNIV
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