The invention relates to a composite lactobacillus preparation capable of resisting helicobacter pylori, reducing blood sugar, conditioning intestines and stomach and enhancing immunity, a preparation method and application thereof. Lactobacillus plantarum MiuCure-01, lactobacillus acidophilus MiuLac-01, lactobacillus casei MiaoTech-01, lactobacillus rhamnosus MiaoTech-11 and streptococcus thermophilus MiaoPro-01 are adopted as raw materials. the invention is prepared by a novel process combining mixed bacteria inoculation, liquid culture, solid-liquid separation, solid culture and the like, the compound lactobacillus with higher activity is obtained, antibacterial substances such as bacteriocin and organic acid generated in the solid culture process are reserved, and the invention has the functions of resisting helicobacter pylori and coordinating intestines and stomach; and various metabolites capable of reducing blood sugar and enhancing immunity can be produced. The solid culture is low in moisture content and easy to dry, stress death of the compound lactobacillus in the drying period can be reduced, and the activity of the compound lactobacillus in the product can be greatly improved by combining proper granulation and coating procedures. The produced composite lactobacillus probiotic preparation has the effects of resisting helicobacter pylori, reducing blood sugar, coordinating intestines and stomach, improving immunity and the like.